Blaschke, Sabine

[["dc.bibliographiccitation.firstpage","1280"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PROTEOMICS - CLINICAL APPLICATIONS"],["dc.bibliographiccitation.lastpage","1284"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Baechle, Daniel"],["dc.contributor.author","Sparbier, Katrin"],["dc.contributor.author","Dihazi, Hassan"],["dc.contributor.author","Blaschke, Sabine"],["dc.contributor.author","Mueller, Gerhard-Anton"],["dc.contributor.author","Kostrzewa, Markus"],["dc.contributor.author","Flad, Thomas"],["dc.date.accessioned","2018-11-07T10:58:23Z"],["dc.date.available","2018-11-07T10:58:23Z"],["dc.date.issued","2007"],["dc.description.abstract","In routine clinical diagnostics, peptide biomarkers are most commonly quantified using immunological techniques but these methods often lack sensitivity and/or specificity. Hence, quantitative mass spectrometry detection is desirable as an alternative diagnostic tool. To date, quantitative mass spectrometry is mostly based on ESI-MS coupled to LC, requiring highly sophisticated instrumentation and knowledge and is time consuming and expensive. In contrast, MALDI-TOF-MS is a very simple, sensitive and rapid method for the detection of peptide biomarkers. However, the infeasibility of absolute quantification has been a tremendous handicap to the use of MS in stable clinical diagnostics. Here, we describe the development of a technical platform based on ClinProt particles and heavy-isotope internal peptide standards for the fast and reliable preparation of samples. This combines the advantages of MALDI-TOF as a read-out system with absolute quantitation of peptide biomarkers. As a proof-of-concept, this platform was successfully employed for the absolute determination of the concentration of the highly abundant serum peptide des-Ala-Fibrinopeptide A in 45 serum samples from healthy donors. Such technology essentially contributes to the development of a stable MALDI-TOF-MS-based clinical assay."],["dc.identifier.doi","10.1002/prca.200700307"],["dc.identifier.isi","000250476600006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50468"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","1862-8346"],["dc.title","Towards stable diagnostic setups in clinical proteomics: Absolute quantitation of peptide biomarkers using MALDI-TOF-MS"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1558"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nephrology Dialysis Transplantation"],["dc.bibliographiccitation.lastpage","1566"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Markovic-Lipkovski, Jasmina"],["dc.contributor.author","Mueller, Claudia A."],["dc.contributor.author","Klein, Gerd"],["dc.contributor.author","Flad, Thomas"],["dc.contributor.author","Klatt, Tatjana"],["dc.contributor.author","Blaschke, Sabine"],["dc.contributor.author","Wessels, Johannes Theodor"],["dc.contributor.author","Mueller, Georg Anton"],["dc.date.accessioned","2018-11-07T11:02:05Z"],["dc.date.available","2018-11-07T11:02:05Z"],["dc.date.issued","2007"],["dc.description.abstract","Background. At early stages of kidney development, the neural cell adhesion molecule (NCAM) is highly expressed on cells of the metanephrogenic mesenchyme. During maturation of the fetal kidney, NCAM gradually disappears. So far, it has been widely accepted that NCAM in the adult kidney is only expressed by nerves, and not by other cell types. Methods. NCAM expression was analysed in human adult healthy and diseased kidneys by immunohistochemistry and western blot analysis. NCAM+ renal interstitial cells were further characterized by double immunofluorescent staining using antibodies against neurofilaments, a smooth muscle actin, vimentin, alpha 5 beta 1 integrin, CD68, CD11c, HLA-DR and the potential progenitor cell markers CD34, CD117, CD133, CD24, nestin and cadherin-11. Results. In adult human kidneys, NCAM expression is restricted to rare interstitial cells with dendritic morphology, which are neurofilament-negative and predominantly localized on the corticomedullary junction. They are also negative for fibroblast cell markers, but co-express the haematopoietic stem cell markers CD34 and CD133. The number of NCAM+ interstitial cells increased in the initial phases of interstitial fibrosis. Western blot analysis of renal tissues with incipient interstitial fibrosis tissues showed the expression of the 140 kDa NCAM isoform. Conclusions. These data indicate that a rare subpopulation of NCAM+ interstitial cells could represent renal progenitors, and that NCAM+ interstitial cells can participate in the initial phase of interstitial fibrosis."],["dc.identifier.doi","10.1093/ndt/gfm006"],["dc.identifier.isi","000247474200012"],["dc.identifier.pmid","17337466"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51294"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.conference","19th European Congress of Pathology"],["dc.relation.eventlocation","Ljubljana, SLOVENIA"],["dc.relation.issn","0931-0509"],["dc.title","Neural cell adhesion molecule expression on renal interstitial cells"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]