Nagel, Holger

[["dc.bibliographiccitation.firstpage","35"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","CANCER GENETICS AND CYTOGENETICS"],["dc.bibliographiccitation.lastpage","47"],["dc.bibliographiccitation.volume","176"],["dc.contributor.author","Schulten, Hans Juergen"],["dc.contributor.author","Perske, Christina"],["dc.contributor.author","Thelen, Paul"],["dc.contributor.author","Polten, Andreas"],["dc.contributor.author","Borst, Christoph"],["dc.contributor.author","Gunawan, Bastian"],["dc.contributor.author","Nagel, Holger"],["dc.date.accessioned","2018-11-07T11:00:53Z"],["dc.date.available","2018-11-07T11:00:53Z"],["dc.date.issued","2007"],["dc.description.abstract","We describe two newly established malignant mesothelioma (MM) cell lines derived from a pleural effusion of a male. One cell line, designated as MM-Z03E, reveals an epithelioid cobblestone morphology, while the second one, designated as MM-Z03S and subcloned after in vivo selection, exhibits a sarcomatoid storiform growth pattern. Both cell lines showed the immunologic profile characteristic for MM (i.e., expression of cytokeratin, CK18, calretinin, and vimentin in both phenotypes). Cytogenetics, multicolor fluorescence in situ hybridization, comparative genomic hybridization, and oligonucleotide array CGH were performed on both cell lines. Aberrations shared by both cell lines included chromosomal losses of 1q34 similar to qter, 4, 9p, 10p, 13, 14, 16q, 18, and 22, as well as a complex structural aberration involving chromosome 17. Aberrations exclusive to MM-Z03E included gains of 3q11q27 and 5p, while gain of 9q and losses of 3q27qter, 11q, and 18 in MM-Z03S were exclusive to MM-Z03E. Both cell lines were able to develop solid transplant tumors in nude mice within 16 weeks, and immunophenotyping of tumor xenografts revealed an overall retained expression profile of the markers used. Remarkably, one xenograft from MM-Z03E revealed overexpression of p53 and widely invasive growth. In conclusion, both cell lines are useful in vivo and in vitro model systems to study the underlying genetic mechanisms of biphasic differentiation in MM, which can be of certain value considering the increasing relevance of assessing MM tumor biology for the clinical management of this disease. (c) 2007 Elsevier Inc. All rights reserved."],["dc.identifier.doi","10.1016/j.cancergencyto.2007.03.005"],["dc.identifier.isi","000247710100004"],["dc.identifier.pmid","17574962"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51027"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","0165-4608"],["dc.title","Establishment and characterization of two distinct malignant mesothelioma cell lines with common clonal origin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","818"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Modern Pathology"],["dc.bibliographiccitation.lastpage","825"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Nagel, H."],["dc.contributor.author","Schulten, Hans-Juergen"],["dc.contributor.author","Gunawan, Bastian"],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Fuzesi, Laszlo"],["dc.date.accessioned","2018-11-07T10:16:55Z"],["dc.date.available","2018-11-07T10:16:55Z"],["dc.date.issued","2002"],["dc.description.abstract","Comparative genomic hybridization (CGH) is a molecular cytogenetic technique that provides an overview on chromosomal imbalances within the whole tumor cell genome. This method has yet not been applied in effusion cytology. We performed CGH analysis in malignant effusions, fine needle aspirates, and imprint smears from eight ovarian adenocarcinomas, three breast carcinomas, one colon adenocarcinoma, and three malignant mesotheliomas. In part, CGH analysis from fresh frozen tissue and classic karyotyping served as controls. In this series, 14/15 cytologic specimens were suitable for extraction of high molecular weight DNA sufficient for reliable CGH analysis. CGH profiles from cytologic material were equal or even more significant in comparison with corresponding fresh frozen tumor samples. We conclude that CGH analysis from cytologic specimens may support the primary cytologic diagnosis of malignancy, especially in the differential diagnosis of benign proliferating mesothelium, malignant mesothelioma, and metastatic adenocarcinoma. CGH analysis of metastatic lesions may provide information on the site of the primary tumors and detects cytogenetic imbalances affecting oncogenes and tumor suppressor genes involved in tumor progression and metastatic spread."],["dc.identifier.doi","10.1097/01.MP.0000024521.67720.0F"],["dc.identifier.isi","000177543800005"],["dc.identifier.pmid","12181266"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41130"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0893-3952"],["dc.title","The potential value of comparative genomic hybridization analysis in effusion - and fine needle aspiration cytology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]