Schmidt, Roman

[["dc.bibliographiccitation.firstpage","539"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","544"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2008"],["dc.description.abstract","The resolution of any linear imaging system is given by its point spread function (PSF) that quantifies the blur of an object point in the image. The sharper the PSF, the better the resolution is. In standard fluorescence microscopy, however, diffraction dictates a PSF with a cigar-shaped main maximum, called the focal spot, which extends over at least half the wavelength of light (lambda= 400 - 700 nm) in the focal plane and >lambda along the optical axis (z). Although concepts have been developed to sharpen the focal spot both laterally and axially, none of them has reached their ultimate goal: a spherical spot that can be arbitrarily downscaled in size. Here we introduce a fluorescence microscope that creates nearly spherical focal spots of 40 - 45 nm (lambda/16) in diameter. Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles."],["dc.identifier.doi","10.1038/nmeth.1214"],["dc.identifier.gro","3143287"],["dc.identifier.isi","000256308200018"],["dc.identifier.pmid","18488034"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/784"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1548-7091"],["dc.title","Spherical nanosized focal spot unravels the interior of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2497"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","2500"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Ullal, Chaitanya K."],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2017-09-07T11:47:27Z"],["dc.date.available","2017-09-07T11:47:27Z"],["dc.date.issued","2009"],["dc.description.abstract","We demonstrate stimulated emission depletion microscopy using opposing objective lenses to noninvasively reveal the nanoscale morphology of block copolymers in three dimensions with focused light. This is exemplified in a poly(styrene-block-2-vinylpyridine) model system in which contrast is achieved by specifically staining the vinylpyridine phase with a fluorescent dye. We image swelling induced mesopores and other convoluted structures within the bulk of samples, at scales that have so far required electron and scanning probe microscopes."],["dc.identifier.doi","10.1021/nl901378e"],["dc.identifier.gro","3143111"],["dc.identifier.isi","000266969400055"],["dc.identifier.pmid","19449834"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/589"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Foundation; Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1530-6984"],["dc.title","Block Copolymer Nanostructures Mapped by Far-Field Optics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","833"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Neuroscience"],["dc.bibliographiccitation.lastpage","839"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Hua, Yunfeng"],["dc.contributor.author","Sinha, Raunak"],["dc.contributor.author","Thiel, Cora S."],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Hüve, Jana"],["dc.contributor.author","Martens, Henrik"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Klingauf, Jurgen"],["dc.date.accessioned","2017-09-07T11:44:10Z"],["dc.date.available","2017-09-07T11:44:10Z"],["dc.date.issued","2011"],["dc.description.abstract","Although clathrin-mediated endocytosis is thought to be the predominant mechanism of synaptic vesicle recycling, it seems to be too slow for fast recycling. Therefore, it was suggested that a presorted and preassembled pool of synaptic vesicle proteins on the presynaptic membrane might support a first wave of fast clathrin-mediated endocytosis. In this study we monitored the temporal dynamics of such a 'readily retrievable pool' of synaptic vesicle proteins in rat hippocampal neurons using a new type of probe. By applying cypHer5E, a new cyanine dye-based pH-sensitive exogenous marker, coupled to antibodies to luminal domains of synaptic vesicle proteins, we could reliably monitor synaptic vesicle recycling and demonstrate the preferential recruitment of a surface pool of synaptic vesicle proteins upon stimulated endocytosis. By using fluorescence nanoscopy of surface-labeled synaptotagmin 1, we could resolve the spatial distribution of the surface pool at the periactive zone in hippocampal boutons, which represent putative sites of endocytosis."],["dc.identifier.doi","10.1038/nn.2838"],["dc.identifier.gro","3142708"],["dc.identifier.isi","000292081700010"],["dc.identifier.pmid","21666673"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/142"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1097-6256"],["dc.title","A readily retrievable pool of synaptic vesicles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10154"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","10167"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Schönle, Andreas"],["dc.date.accessioned","2017-09-07T11:46:04Z"],["dc.date.available","2017-09-07T11:46:04Z"],["dc.date.issued","2010"],["dc.description.abstract","4Pi-microscopy doubles the aperture of the imaging system by coherent addition of the wavefronts for illumination and/or detection through opposing objective lenses. This improves the axial resolution 3-7 fold, but the raw data usually features ghost images which have to be removed by image reconstruction. This straightforward procedure is sometimes precluded by imperfect alignment of the instrument or a specimen with strong variations of its refractive index, because the image formation process now depends on the space-variant phase difference between the counter-propagating wavefronts. Here we present a computationally fast method of parametric blind deconvolution that allows for automatic and robust simultaneous estimation of both the object and the phase function in such cases. We verify the performance of our approach on both synthetic and real data. Because the method does not require a-priori knowledge of the phase function it is a major step towards reliable 4Pi-imaging and automatic image restoration by non-expert users."],["dc.identifier.doi","10.1364/OE.18.010154"],["dc.identifier.gro","3142926"],["dc.identifier.isi","000277560000042"],["dc.identifier.pmid","20588870"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/384"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: German Federal Ministry of Education and Research"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Automatic deconvolution in 4Pi-microscopy with variable phase"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","381"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","387"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2017-09-07T11:46:53Z"],["dc.date.available","2017-09-07T11:46:53Z"],["dc.date.issued","2009"],["dc.description.abstract","The resolution of far-field optical microscopy stagnated for a century, but a quest began in the 1990s leading to nanoscale imaging of transparent fluorescent objects in three dimensions. Important elements in this pursuit were the synthesis of the aperture of two opposing lenses and the modulation or switching of the fluorescence of adjacent markers. The first element provided nearly isotropic three-dimensional resolution by improving the axial resolution by three-to sevenfold, and the second enabled the diffraction barrier to be overcome. Here, we review recent progress in the synergistic combination of these two elements which non-invasively provide an isotropic diffraction-unlimited three-dimensional resolution in transparent objects."],["dc.identifier.doi","10.1038/NPHOTON.2009.112"],["dc.identifier.gro","3143091"],["dc.identifier.isi","000268067700012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/566"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1749-4885"],["dc.title","Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","7508"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Macromolecules"],["dc.bibliographiccitation.lastpage","7510"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Ullal, Chaitanya K."],["dc.contributor.author","Primpke, Sebastian"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Böhm, Ulrike"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Vana, Philipp"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:43:20Z"],["dc.date.available","2017-09-07T11:43:20Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1021/ma201504f"],["dc.identifier.gro","3142648"],["dc.identifier.isi","000295487600003"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9448"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0024-9297"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Flexible Microdomain Specific Staining of Block Copolymers for 3D Optical Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2508"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","2510"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Punge, Annedore"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:47:27Z"],["dc.date.available","2017-09-07T11:47:27Z"],["dc.date.issued","2009"],["dc.description.abstract","Because of the diffraction resolution barrier, optical microscopes have so far failed in visualizing the mitochondrial cristae, that is, the folds of the inner membrane of this 200 to 400 nm diameter sized tubular organelle. Realizing a similar to 30 nm isotropic subdiffraction resolution in isoSTED fluorescence nanoscopy, we have visualized these essential structures in the mitochondria of intact cells. We find a pronounced heterogeneity in the cristae arrangements even within individual mitochondrial tubules."],["dc.identifier.doi","10.1021/nl901398t"],["dc.identifier.gro","3143112"],["dc.identifier.isi","000266969400057"],["dc.identifier.pmid","19459703"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/590"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [JA 1129/3, SFB 755]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1530-6984"],["dc.title","Mitochondrial Cristae Revealed with Focused Light"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10920"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Langmuir"],["dc.bibliographiccitation.lastpage","10928"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Kumar, Karthik"],["dc.contributor.author","Isa, Lucio"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Textor, Marcus"],["dc.contributor.author","Reimhult, Erik"],["dc.date.accessioned","2022-03-01T11:45:43Z"],["dc.date.available","2022-03-01T11:45:43Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1021/la2019132"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103429"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1520-5827"],["dc.relation.issn","0743-7463"],["dc.title","Formation of Nanopore-Spanning Lipid Bilayers through Liposome Fusion"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1855"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1863"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Okamura, Yosuke"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Raschke, Ines"],["dc.contributor.author","Hintze, Maik"],["dc.contributor.author","Takeoka, Shinji"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Lang, Thorsten"],["dc.date.accessioned","2022-03-01T11:44:54Z"],["dc.date.available","2022-03-01T11:44:54Z"],["dc.date.issued","2011"],["dc.identifier.doi","10.1016/j.bpj.2011.02.052"],["dc.identifier.pii","S0006349511003055"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103154"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","A Few Immobilized Thrombins Are Sufficient for Platelet Spreading"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]