Schmidt, Roman

[["dc.bibliographiccitation.firstpage","539"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","544"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2008"],["dc.description.abstract","The resolution of any linear imaging system is given by its point spread function (PSF) that quantifies the blur of an object point in the image. The sharper the PSF, the better the resolution is. In standard fluorescence microscopy, however, diffraction dictates a PSF with a cigar-shaped main maximum, called the focal spot, which extends over at least half the wavelength of light (lambda= 400 - 700 nm) in the focal plane and >lambda along the optical axis (z). Although concepts have been developed to sharpen the focal spot both laterally and axially, none of them has reached their ultimate goal: a spherical spot that can be arbitrarily downscaled in size. Here we introduce a fluorescence microscope that creates nearly spherical focal spots of 40 - 45 nm (lambda/16) in diameter. Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles."],["dc.identifier.doi","10.1038/nmeth.1214"],["dc.identifier.gro","3143287"],["dc.identifier.isi","000256308200018"],["dc.identifier.pmid","18488034"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/784"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1548-7091"],["dc.title","Spherical nanosized focal spot unravels the interior of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","30891"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","30903"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Curdt, Franziska"],["dc.contributor.author","Herr, Simon J."],["dc.contributor.author","Lutz, Tobias"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:52Z"],["dc.date.available","2017-09-07T11:54:52Z"],["dc.date.issued","2015"],["dc.description.abstract","Despite the need for isotropic optical resolution in a growing number of applications, the majority of super-resolution fluorescence microscopy setups still do not attain an axial resolution comparable to that in the lateral dimensions. Three-dimensional (3D) nanoscopy implementations that employ only a single objective lens typically feature a trade-off between axial and lateral resolution. 4Pi arrangements, in which the sample is illuminated coherently through two opposing lenses, have proven their potential for rendering the resolution isotropic. However, instrument complexity due to a large number of alignment parameters has so far thwarted the dissemination of this approach. Here, we present a 4Pi-STED setup combination, also called isoSTED nanoscope, where the STED and excitation beams are intrinsically co-aligned. A highly robust and convenient 4Pi cavity allows easy handling without the need for readjustments during imaging experiments. (c) 2015 Optical Society of America"],["dc.identifier.doi","10.1364/OE.23.030891"],["dc.identifier.gro","3141784"],["dc.identifier.isi","000366614100066"],["dc.identifier.pmid","26698722"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1035"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: German Federal Ministry of Education and Research (BMBF) [FKZ: 13N11173]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","isoSTED nanoscopy with intrinsic beam alignment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]