Schmidt, Roman

[["dc.bibliographiccitation.artnumber","10504"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Böhm, Ulrike"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Schmidt, Roman"],["dc.date.accessioned","2017-09-07T11:54:40Z"],["dc.date.available","2017-09-07T11:54:40Z"],["dc.date.issued","2016"],["dc.description.abstract","By enlarging the aperture along the optic axis, the coherent utilization of opposing objective lenses (4Pi arrangement) has the potential to offer the sharpest and most light-efficient point-spread-functions in three-dimensional (3D) far-field fluorescence nanoscopy. However, to obtain unambiguous images, the signal has to be discriminated against contributions from lobes above and below the focal plane, which has tentatively limited 4Pi arrangements to imaging samples with controllable optical conditions. Here we apply the 4Pi scheme to RESOLFT nanoscopy using two-photon absorption for the on-switching of fluorescent proteins. We show that in this combination, the lobes are so low that low-light level, 3D nanoscale imaging of living cells becomes possible. Our method thus offers robust access to densely packed, axially extended cellular regions that have been notoriously difficult to super-resolve. Our approach also entails a fluorescence read-out scheme that translates molecular sensitivity to local off-switching rates into improved signal-to-noise ratio and resolution."],["dc.identifier.doi","10.1038/ncomms10504"],["dc.identifier.gro","3141732"],["dc.identifier.isi","000371012500001"],["dc.identifier.pmid","26833381"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/458"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Deutsche Forschungsgemeinschaft [SFB 775]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2041-1723"],["dc.title","4Pi-RESOLFT nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","434"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Der Urologe"],["dc.bibliographiccitation.lastpage","443"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Graf, S."],["dc.contributor.author","Kranz, J."],["dc.contributor.author","Schmidt, S."],["dc.contributor.author","Bellut, L."],["dc.contributor.author","Uhlig, A."],["dc.date.accessioned","2021-04-14T08:29:20Z"],["dc.date.available","2021-04-14T08:29:20Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1007/s00120-021-01476-x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82868"],["dc.language.iso","de"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1433-0563"],["dc.relation.issn","0340-2592"],["dc.title","Formen der Evidenzsynthese"],["dc.title.translated","Types of evidence syntheses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e040119"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","BMJ Open"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Otte, Christian"],["dc.contributor.author","Chae, Woo Ri"],["dc.contributor.author","Nowacki, Jan"],["dc.contributor.author","Kaczmarczyk, Michael"],["dc.contributor.author","Piber, Dominique"],["dc.contributor.author","Roepke, Stefan"],["dc.contributor.author","Märschenz, Stefanie"],["dc.contributor.author","Lischewski, Sandra"],["dc.contributor.author","Schmidt, Sein"],["dc.contributor.author","Ettrich, Barbara"],["dc.contributor.author","Grabe, Hans Joergen"],["dc.contributor.author","Hegerl, Ulrich"],["dc.contributor.author","Hinkelmann, Kim"],["dc.contributor.author","Hofmann, Tobias"],["dc.contributor.author","Janowitz, Deborah"],["dc.contributor.author","Junghanns, Klaus"],["dc.contributor.author","Kahl, Kai G."],["dc.contributor.author","Klein, Jan Philipp"],["dc.contributor.author","Krueger, Tillmann H. C."],["dc.contributor.author","Leicht, Gregor"],["dc.contributor.author","Prvulovic, David"],["dc.contributor.author","Reif, Andreas"],["dc.contributor.author","Schoettle, Daniel"],["dc.contributor.author","Strauss, Maria"],["dc.contributor.author","Westermair, Anna"],["dc.contributor.author","Friede, Tim"],["dc.contributor.author","Gold, Stefan M"],["dc.date.accessioned","2021-04-14T08:27:25Z"],["dc.date.available","2021-04-14T08:27:25Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1136/bmjopen-2020-040119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82287"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2044-6055"],["dc.relation.issn","2044-6055"],["dc.title","Simvastatin add-on to escitalopram in patients with comorbid obesity and major depression (SIMCODE): study protocol of a multicentre, randomised, double-blind, placebo-controlled trial"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","539"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","544"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2008"],["dc.description.abstract","The resolution of any linear imaging system is given by its point spread function (PSF) that quantifies the blur of an object point in the image. The sharper the PSF, the better the resolution is. In standard fluorescence microscopy, however, diffraction dictates a PSF with a cigar-shaped main maximum, called the focal spot, which extends over at least half the wavelength of light (lambda= 400 - 700 nm) in the focal plane and >lambda along the optical axis (z). Although concepts have been developed to sharpen the focal spot both laterally and axially, none of them has reached their ultimate goal: a spherical spot that can be arbitrarily downscaled in size. Here we introduce a fluorescence microscope that creates nearly spherical focal spots of 40 - 45 nm (lambda/16) in diameter. Fully relying on focused light, this lens-based fluorescence nanoscope unravels the interior of cells noninvasively, uniquely dissecting their sub-lambda-sized organelles."],["dc.identifier.doi","10.1038/nmeth.1214"],["dc.identifier.gro","3143287"],["dc.identifier.isi","000256308200018"],["dc.identifier.pmid","18488034"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/784"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1548-7091"],["dc.title","Spherical nanosized focal spot unravels the interior of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","30891"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","30903"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Curdt, Franziska"],["dc.contributor.author","Herr, Simon J."],["dc.contributor.author","Lutz, Tobias"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:52Z"],["dc.date.available","2017-09-07T11:54:52Z"],["dc.date.issued","2015"],["dc.description.abstract","Despite the need for isotropic optical resolution in a growing number of applications, the majority of super-resolution fluorescence microscopy setups still do not attain an axial resolution comparable to that in the lateral dimensions. Three-dimensional (3D) nanoscopy implementations that employ only a single objective lens typically feature a trade-off between axial and lateral resolution. 4Pi arrangements, in which the sample is illuminated coherently through two opposing lenses, have proven their potential for rendering the resolution isotropic. However, instrument complexity due to a large number of alignment parameters has so far thwarted the dissemination of this approach. Here, we present a 4Pi-STED setup combination, also called isoSTED nanoscope, where the STED and excitation beams are intrinsically co-aligned. A highly robust and convenient 4Pi cavity allows easy handling without the need for readjustments during imaging experiments. (c) 2015 Optical Society of America"],["dc.identifier.doi","10.1364/OE.23.030891"],["dc.identifier.gro","3141784"],["dc.identifier.isi","000366614100066"],["dc.identifier.pmid","26698722"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1035"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: German Federal Ministry of Education and Research (BMBF) [FKZ: 13N11173]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","isoSTED nanoscopy with intrinsic beam alignment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2497"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Nano Letters"],["dc.bibliographiccitation.lastpage","2500"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Ullal, Chaitanya K."],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2017-09-07T11:47:27Z"],["dc.date.available","2017-09-07T11:47:27Z"],["dc.date.issued","2009"],["dc.description.abstract","We demonstrate stimulated emission depletion microscopy using opposing objective lenses to noninvasively reveal the nanoscale morphology of block copolymers in three dimensions with focused light. This is exemplified in a poly(styrene-block-2-vinylpyridine) model system in which contrast is achieved by specifically staining the vinylpyridine phase with a fluorescent dye. We image swelling induced mesopores and other convoluted structures within the bulk of samples, at scales that have so far required electron and scanning probe microscopes."],["dc.identifier.doi","10.1021/nl901378e"],["dc.identifier.gro","3143111"],["dc.identifier.isi","000266969400055"],["dc.identifier.pmid","19449834"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/589"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Foundation; Deutsche Forschungsgemeinschaft [SFB 755]"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1530-6984"],["dc.title","Block Copolymer Nanostructures Mapped by Far-Field Optics"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","833"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Neuroscience"],["dc.bibliographiccitation.lastpage","839"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Hua, Yunfeng"],["dc.contributor.author","Sinha, Raunak"],["dc.contributor.author","Thiel, Cora S."],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Hüve, Jana"],["dc.contributor.author","Martens, Henrik"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Klingauf, Jurgen"],["dc.date.accessioned","2017-09-07T11:44:10Z"],["dc.date.available","2017-09-07T11:44:10Z"],["dc.date.issued","2011"],["dc.description.abstract","Although clathrin-mediated endocytosis is thought to be the predominant mechanism of synaptic vesicle recycling, it seems to be too slow for fast recycling. Therefore, it was suggested that a presorted and preassembled pool of synaptic vesicle proteins on the presynaptic membrane might support a first wave of fast clathrin-mediated endocytosis. In this study we monitored the temporal dynamics of such a 'readily retrievable pool' of synaptic vesicle proteins in rat hippocampal neurons using a new type of probe. By applying cypHer5E, a new cyanine dye-based pH-sensitive exogenous marker, coupled to antibodies to luminal domains of synaptic vesicle proteins, we could reliably monitor synaptic vesicle recycling and demonstrate the preferential recruitment of a surface pool of synaptic vesicle proteins upon stimulated endocytosis. By using fluorescence nanoscopy of surface-labeled synaptotagmin 1, we could resolve the spatial distribution of the surface pool at the periactive zone in hippocampal boutons, which represent putative sites of endocytosis."],["dc.identifier.doi","10.1038/nn.2838"],["dc.identifier.gro","3142708"],["dc.identifier.isi","000292081700010"],["dc.identifier.pmid","21666673"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/142"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1097-6256"],["dc.title","A readily retrievable pool of synaptic vesicles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10154"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","10167"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Schönle, Andreas"],["dc.date.accessioned","2017-09-07T11:46:04Z"],["dc.date.available","2017-09-07T11:46:04Z"],["dc.date.issued","2010"],["dc.description.abstract","4Pi-microscopy doubles the aperture of the imaging system by coherent addition of the wavefronts for illumination and/or detection through opposing objective lenses. This improves the axial resolution 3-7 fold, but the raw data usually features ghost images which have to be removed by image reconstruction. This straightforward procedure is sometimes precluded by imperfect alignment of the instrument or a specimen with strong variations of its refractive index, because the image formation process now depends on the space-variant phase difference between the counter-propagating wavefronts. Here we present a computationally fast method of parametric blind deconvolution that allows for automatic and robust simultaneous estimation of both the object and the phase function in such cases. We verify the performance of our approach on both synthetic and real data. Because the method does not require a-priori knowledge of the phase function it is a major step towards reliable 4Pi-imaging and automatic image restoration by non-expert users."],["dc.identifier.doi","10.1364/OE.18.010154"],["dc.identifier.gro","3142926"],["dc.identifier.isi","000277560000042"],["dc.identifier.pmid","20588870"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/384"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: German Federal Ministry of Education and Research"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Automatic deconvolution in 4Pi-microscopy with variable phase"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1478"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Weihs, Tobias"],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Jansen, Isabelle"],["dc.contributor.author","Rehman, Jasmin"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:45:59Z"],["dc.date.available","2022-03-01T11:45:59Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract The recently introduced minimal photon fluxes (MINFLUX) concept pushed the resolution of fluorescence microscopy to molecular dimensions. Initial demonstrations relied on custom made, specialized microscopes, raising the question of the method’s general availability. Here, we show that MINFLUX implemented with a standard microscope stand can attain 1–3 nm resolution in three dimensions, rendering fluorescence microscopy with molecule-scale resolution widely applicable. Advances, such as synchronized electro-optical and galvanometric beam steering and a stabilization that locks the sample position to sub-nanometer precision with respect to the stand, ensure nanometer-precise and accurate real-time localization of individually activated fluorophores. In our MINFLUX imaging of cell- and neurobiological samples, ~800 detected photons suffice to attain a localization precision of 2.2 nm, whereas ~2500 photons yield precisions <1 nm (standard deviation). We further demonstrate 3D imaging with localization precision of ~2.4 nm in the focal plane and ~1.9 nm along the optic axis. Localizing with a precision of <20 nm within ~100 µs, we establish this spatio-temporal resolution in single fluorophore tracking and apply it to the diffusion of single labeled lipids in lipid-bilayer model membranes."],["dc.description.abstract","Abstract The recently introduced minimal photon fluxes (MINFLUX) concept pushed the resolution of fluorescence microscopy to molecular dimensions. Initial demonstrations relied on custom made, specialized microscopes, raising the question of the method’s general availability. Here, we show that MINFLUX implemented with a standard microscope stand can attain 1–3 nm resolution in three dimensions, rendering fluorescence microscopy with molecule-scale resolution widely applicable. Advances, such as synchronized electro-optical and galvanometric beam steering and a stabilization that locks the sample position to sub-nanometer precision with respect to the stand, ensure nanometer-precise and accurate real-time localization of individually activated fluorophores. In our MINFLUX imaging of cell- and neurobiological samples, ~800 detected photons suffice to attain a localization precision of 2.2 nm, whereas ~2500 photons yield precisions <1 nm (standard deviation). We further demonstrate 3D imaging with localization precision of ~2.4 nm in the focal plane and ~1.9 nm along the optic axis. Localizing with a precision of <20 nm within ~100 µs, we establish this spatio-temporal resolution in single fluorophore tracking and apply it to the diffusion of single labeled lipids in lipid-bilayer model membranes."],["dc.identifier.doi","10.1038/s41467-021-21652-z"],["dc.identifier.pii","21652"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103518"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","2041-1723"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","381"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Photonics"],["dc.bibliographiccitation.lastpage","387"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Schmidt, Roman"],["dc.contributor.author","Egner, Alexander"],["dc.date.accessioned","2017-09-07T11:46:53Z"],["dc.date.available","2017-09-07T11:46:53Z"],["dc.date.issued","2009"],["dc.description.abstract","The resolution of far-field optical microscopy stagnated for a century, but a quest began in the 1990s leading to nanoscale imaging of transparent fluorescent objects in three dimensions. Important elements in this pursuit were the synthesis of the aperture of two opposing lenses and the modulation or switching of the fluorescence of adjacent markers. The first element provided nearly isotropic three-dimensional resolution by improving the axial resolution by three-to sevenfold, and the second enabled the diffraction barrier to be overcome. Here, we review recent progress in the synergistic combination of these two elements which non-invasively provide an isotropic diffraction-unlimited three-dimensional resolution in transparent objects."],["dc.identifier.doi","10.1038/NPHOTON.2009.112"],["dc.identifier.gro","3143091"],["dc.identifier.isi","000268067700012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/566"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1749-4885"],["dc.title","Diffraction-unlimited three-dimensional optical nanoscopy with opposing lenses"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]