Punge, Annedore

[["dc.bibliographiccitation.firstpage","19055"],["dc.bibliographiccitation.issue","44"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","19060"],["dc.bibliographiccitation.volume","107"],["dc.contributor.author","Hoopmann, Peer"],["dc.contributor.author","Punge, Annedore"],["dc.contributor.author","Barysch, Sina Victoria"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Bückers, Johanna"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Bethani, Ioanna"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:45:12Z"],["dc.date.available","2017-09-07T11:45:12Z"],["dc.date.issued","2010"],["dc.description.abstract","Neurotransmitter release is achieved through the fusion of synaptic vesicles with the neuronal plasma membrane (exocytosis). Vesicles are then retrieved from the plasma membrane (endocytosis). It was hypothesized more than 3 decades ago that endosomes participate in vesicle recycling, constituting a slow endocytosis pathway required especially after prolonged stimulation. This recycling model predicts that newly endocytosed vesicles fuse with an endosome, which sorts (organizes) the molecules and buds exocytosis-competent vesicles. We analyzed here the endosome function using hippocampal neurons, isolated nerve terminals (synaptosomes), and PC12 cells by stimulated emission depletion microscopy, photooxidation EM, and several conventional microscopy assays. Surprisingly, we found that endosomal sorting is a rapid pathway, which appeared to be involved in the recycling of the initial vesicles to be released on stimulation, the readily releasable pool. In agreement with the endosomal model, the vesicle composition changed after endocytosis, with the newly formed vesicles being enriched in plasma membrane proteins. Vesicle proteins were organized in clusters both in the plasma membrane (on exocytosis) and in the endosome. In the latter compartment, they segregated from plasma membrane components in a process that is likely important for sorting/budding of newly developed vesicles from the endosome."],["dc.identifier.doi","10.1073/pnas.1007037107"],["dc.identifier.gro","3142833"],["dc.identifier.isi","000283749000058"],["dc.identifier.pmid","20956291"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/281"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0027-8424"],["dc.title","Endosomal sorting of readily releasable synaptic vesicles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","800"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Traffic"],["dc.bibliographiccitation.lastpage","812"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Punge, Annedore"],["dc.contributor.author","Bückers, Johanna"],["dc.contributor.author","Hoopmann, Peer"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:46:01Z"],["dc.date.available","2017-09-07T11:46:01Z"],["dc.date.issued","2010"],["dc.description.abstract","Synaptic vesicles recycle repeatedly in order to maintain synaptic transmission. We have previously proposed that upon exocytosis the vesicle components persist as clusters, which would be endocytosed as whole units. It has also been proposed that the vesicle components diffuse into the plasma membrane and are then randomly gathered into new vesicles. We found here that while strong stimulation (releasing the entire recycling pool) causes the diffusion of the vesicle marker synaptotagmin out of synaptic boutons, moderate stimulation (releasing similar to 19% of all vesicles) is followed by no measurable diffusion. In agreement with this observation, synaptotagmin molecules labeled with different fluorescently tagged antibodies did not appear to mix upon vesicle recycling, when investigated by subdiffraction resolution stimulated emission depletion (STED) microscopy. Finally, as protein diffusion from vesicles has been mainly observed using molecules tagged with pH-sensitive green fluorescent protein (pHluorin), we have also investigated the membrane patterning of several native and pHluorin-tagged proteins. While the native proteins had a clustered distribution, the GFP-tagged ones were diffused in the plasma membrane. We conclude that synaptic vesicle components intermix little, at least under moderate stimulation, possibly because of the formation of clusters in the plasma membrane. We suggest that several pHluorin-tagged vesicle proteins are less well integrated in clusters."],["dc.identifier.doi","10.1111/j.1600-0854.2010.01058.x"],["dc.identifier.gro","3142920"],["dc.identifier.isi","000277529300007"],["dc.identifier.pmid","20230528"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/377"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1398-9219"],["dc.title","Limited Intermixing of Synaptic Vesicle Components upon Vesicle Recycling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]