Goyal, Akanksha

[["dc.bibliographiccitation.firstpage","3113"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Cell reports"],["dc.bibliographiccitation.lastpage","3122"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Goyal, Akanksha"],["dc.contributor.author","Belardinelli, Riccardo"],["dc.contributor.author","Rodnina, Marina V."],["dc.date.accessioned","2018-01-17T12:51:50Z"],["dc.date.available","2018-01-17T12:51:50Z"],["dc.date.issued","2017-09-26"],["dc.description.abstract","Canonical translation initiation in bacteria entails the assembly of the 30S initiation complex (IC), which binds the 50S subunit to form a 70S IC. IF3, a key initiation factor, is recruited to the 30S subunit at an early stage and is displaced from its primary binding site upon subunit joining. We employed four different FRET pairs to monitor IF3 relocation after 50S joining. IF3 moves away from the 30S subunit, IF1 and IF2, but can remain bound to the mature 70S IC. The secondary binding site is located on the 50S subunit in the vicinity of ribosomal protein L33. The interaction between IF3 and the 50S subunit is largely electrostatic with very high rates of IF3 binding and dissociation. The existence of the non-canonical binding site may help explain how IF3 participates in alternative initiation modes performed directly by the 70S ribosomes, such as initiation on leaderless mRNAs or re-initiation."],["dc.identifier.doi","10.1016/j.celrep.2017.09.012"],["dc.identifier.pmid","28954228"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11690"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.relation.eissn","2211-1247"],["dc.title","Non-canonical Binding Site for Bacterial Initiation Factor 3 on the Large Ribosomal Subunit"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10700"],["dc.bibliographiccitation.issue","22"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","10712"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Goyal, Akanksha"],["dc.contributor.author","Belardinelli, Riccardo"],["dc.contributor.author","Maracci, Cristina"],["dc.contributor.author","Milón, Pohl"],["dc.contributor.author","Rodnina, Marina"],["dc.date.accessioned","2017-09-07T11:54:48Z"],["dc.date.available","2017-09-07T11:54:48Z"],["dc.date.issued","2015"],["dc.description.abstract","The transition of the 30S initiation complex (IC) to the translating 70S ribosome after 50S subunit joining provides an important checkpoint for mRNA selection during translation in bacteria. Here, we study the timing and control of reactions that occur during 70S IC formation by rapid kinetic techniques, using a toolbox of fluorescence-labeled translation components. We present a kinetic model based on global fitting of time courses obtained with eight different reporters at increasing concentrations of 50S subunits. IF1 and IF3 together affect the kinetics of subunit joining, but do not alter the elemental rates of subsequent steps of 70S IC maturation. After 50S subunit joining, IF2-dependent reactions take place independent of the presence of IF1 or IF3. GTP hydrolysis triggers the efficient dissociation of fMet-tRNA(fMet) from IF2 and promotes the dissociation of IF2 and IF1 from the 70S IC, but does not affect IF3. The presence of non-hydrolyzable GTP analogs shifts the equilibrium towards a stable 70S-mRNA-IF1-IF2-fMet-tRNA(fMet) complex. Our kinetic analysis reveals the molecular choreography of the late stages in translation initiation."],["dc.identifier.doi","10.1093/nar/gkv869"],["dc.identifier.gro","3141765"],["dc.identifier.isi","000371237600020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/824"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1362-4962"],["dc.relation.issn","0305-1048"],["dc.title","Directional transition from initiation to elongation in bacterial translation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]