Köster, Sarah

[["dc.bibliographiccitation.firstpage","1220"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","ChemPhysChem"],["dc.bibliographiccitation.lastpage","1223"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Graceffa, Rita"],["dc.contributor.author","Hémonnot, Clément Y. J."],["dc.contributor.author","Ranke, Christiane"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Liebi, Marianne"],["dc.contributor.author","Marmiroli, Benedetta"],["dc.contributor.author","Weihausen, Britta"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2018-02-12T12:27:30Z"],["dc.date.available","2018-02-12T12:27:30Z"],["dc.date.issued","2017"],["dc.description.abstract","Encapsulating reacting biological or chemical samples in microfluidic droplets has the great advantage over single‐phase flows of providing separate reaction compartments. These compartments can be filled in a combinatoric way and prevent the sample from adsorbing to the channel walls. In recent years, small‐angle X‐ray scattering (SAXS) in combination with microfluidics has evolved as a nanoscale method of such systems. Here, we approach two major challenges associated with combining droplet microfluidics and SAXS. First, we present a simple, versatile, and reliable device, which is both suitable for stable droplet formation and compatible with in situ X‐ray measurements. Second, we solve the problem of “diluting” the sample signal by the signal from the oil separating the emulsion droplets by multiple fast acquisitions per droplet and data thresholding. We show that using our method, even the weakly scattering protein vimentin provides high signal‐to‐noise ratio data."],["dc.identifier.doi","10.1002/cphc.201700221"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12175"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","cytoskeleton"],["dc.subject.gro","molecular biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Rapid Acquisition of X-Ray Scattering Data from Droplet-Encapsulated Protein Systems"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Cassini, Chiara"],["dc.contributor.author","Wittmeier, Andrew"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Denz, Manuela"],["dc.contributor.author","Burghammer, Manfred"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2020-06-26T11:08:18Z"],["dc.date.available","2020-06-26T11:08:18Z"],["dc.date.issued","2020"],["dc.description.abstract","X-ray imaging is a complementary method to electron and fluorescence\r\nmicroscopy for studying biological cells. In particular, scanning small-angle\r\nX-ray scattering provides overview images of whole cells in real space as well as\r\nlocal, high-resolution reciprocal space information, rendering it suitable to\r\ninvestigate subcellular nanostructures in unsliced cells. One persisting challenge\r\nin cell studies is achieving high throughput in reasonable times. To this end,\r\na fast scanning mode is used to image hundreds of cells in a single scan. A way\r\nof dealing with the vast amount of data thus collected is suggested, including\r\na segmentation procedure and three complementary kinds of analysis, i.e.\r\ncharacterization of the cell population as a whole, of single cells and of different\r\nparts of the same cell. The results show that short exposure times, which enable\r\nfaster scans and reduce radiation damage, still yield information in agreement\r\nwith longer exposure times."],["dc.identifier.doi","10.1107/S1600577520006864"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66753"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/48"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY 4.0"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","x-ray scattering"],["dc.title","Large field-of-view scanning small-angle X-ray scattering of mammalian cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4142"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","4154"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Komorowski, Karlo"],["dc.contributor.author","Sztucki, Michael"],["dc.contributor.author","Sharpnack, Lewis"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Schaeper, Jannis"],["dc.date.accessioned","2020-04-23T12:28:28Z"],["dc.date.available","2020-04-23T12:28:28Z"],["dc.date.issued","2020"],["dc.description.abstract","We have used time-resolved small-angle X-ray scattering (SAXS) to study the adhesion of lipid vesicles in the electrostatic strong-coupling regime induced by divalent ions. The bilayer structure and the interbilayer distance dw between adhered vesicles was studied for different DOPC:DOPS mixtures varying the surface charge density of the membrane, as well as for different divalent ions, such as Ca2+, Sr2+, and Zn2+. The results are in good agreement with the strong coupling theory predicting the adhesion state and the corresponding like-charge attraction based on ion-correlations. Using SAXS combined with the stopped-flow rapid mixing technique, we find that in highly charged bilayers the adhesion state is only of transient nature, and that the adhering vesicles subsequently transform to a phase of multilamellar vesicles, again with an inter-bilayer distance according to the theory of strong binding. Aside from the stopped-flow SAXS instrumentations used primarily for these results, we also evaluate microfluidic sample environments for vesicle SAXS in view of future extension of this work."],["dc.identifier.doi","10.1039/D0SM00259C"],["dc.identifier.pmid","32319505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64290"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/186"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/72"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A02: Bestimmung der Struktur synaptischer Organellen durch Röntgenbeugungs- und Bildgebungsverfahren"],["dc.relation","SFB 1286 | B02: Ein in vitro-Verfahren zum Verständnis der struktur-organisierenden Rolle des Vesikel-Clusters"],["dc.relation.eissn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC 3.0"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","membrane biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Vesicle adhesion in the electrostatic strong-coupling regime studied by time-resolved small-angle X-ray scattering"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Neurobiology"],["dc.contributor.author","Epple, Robert"],["dc.contributor.author","Krüger, Dennis"],["dc.contributor.author","Berulava, Tea"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Ninov, Momchil"],["dc.contributor.author","Islam, Rezaul"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Fischer, Andre"],["dc.date.accessioned","2021-04-14T08:29:06Z"],["dc.date.available","2021-04-14T08:29:06Z"],["dc.date.issued","2021"],["dc.description.abstract","Neurons are highly compartmentalized cells that depend on local protein synthesis. Messenger RNAs (mRNAs) have thus been detected in neuronal dendrites, and more recently in the pre- and postsynaptic compartments as well. Other RNA species such as microRNAs have also been described at synapses where they are believed to control mRNA availability for local translation. A combined dataset analyzing the synaptic coding and non-coding RNAome via next-generation sequencing approaches is, however, still lacking. Here, we isolate synaptosomes from the hippocampus of young wild-type mice and provide the coding and non-coding synaptic RNAome. These data are complemented by a novel approach for analyzing the synaptic RNAome from primary hippocampal neurons grown in microfluidic chambers. Our data show that synaptic microRNAs control almost the entire synaptic mRNAome, and we identified several hub microRNAs. By combining the in vivo synaptosomal data with our novel microfluidic chamber system, our findings also support the hypothesis that part of the synaptic microRNAome may be supplied to neurons via astrocytes. Moreover, the microfluidic system is suitable for studying the dynamics of the synaptic RNAome in response to stimulation. In conclusion, our data provide a valuable resource and point to several important targets for further research."],["dc.identifier.doi","10.1007/s12035-021-02296-y"],["dc.identifier.pmid","33569760"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82797"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/228"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/110"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B06: Die Rolle von RNA in Synapsenphysiologie und Neurodegeneration"],["dc.relation.eissn","1559-1182"],["dc.relation.haserratum","/handle/2/82796"],["dc.relation.issn","0893-7648"],["dc.relation.workinggroup","RG A. Fischer (Epigenetics and Systems Medicine in Neurodegenerative Diseases)"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY 4.0"],["dc.title","The Coding and Small Non-coding Hippocampal Synaptic RNAome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Neurobiology"],["dc.contributor.author","Epple, Robert"],["dc.contributor.author","Krüger, Dennis"],["dc.contributor.author","Berulava, Tea"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Ninov, Momchil"],["dc.contributor.author","Islam, Rezaul"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Fischer, Andre"],["dc.date.accessioned","2021-04-14T08:29:05Z"],["dc.date.available","2021-04-14T08:29:05Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1007/s12035-021-02349-2"],["dc.identifier.pmid","33710584"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82796"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/273"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1559-1182"],["dc.relation.isdataof","/handle/2/82797"],["dc.relation.issn","0893-7648"],["dc.relation.workinggroup","RG A. Fischer (Epigenetics and Systems Medicine in Neurodegenerative Diseases)"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.rights","CC BY 4.0"],["dc.title","Correction to: The Coding and Small Non-coding Hippocampal Synaptic RNAome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","171"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Lab on a Chip"],["dc.bibliographiccitation.lastpage","178"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Denz, Manuela"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Hémonnot, Clément Y. J."],["dc.contributor.author","Spears, Heidi"],["dc.contributor.author","Wittmeier, Andrew"],["dc.contributor.author","Cassini, Chiara"],["dc.contributor.author","Saldanha, Oliva"],["dc.contributor.author","Perego, Eleonora"],["dc.contributor.author","Diaz, Ana"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Burghammer, Manfred"],["dc.date.accessioned","2021-06-01T10:50:48Z"],["dc.date.available","2021-06-01T10:50:48Z"],["dc.date.issued","2018"],["dc.description.abstract","The combination of microfluidics and X-ray methods attracts a lot of attention from researchers as it brings together the high controllability of microfluidic sample environments and the small length scales probed by X-rays. In particular, the fields of biophysics and biology have benefited enormously from such approaches. We introduce a straightforward fabrication method for X-ray compatible microfluidic devices made solely from cyclic olefin copolymers. We benchmark the performance of the devices against other devices including more commonly used Kapton windows and obtain data of equal quality using small angle X-ray scattering. An advantage of the devices presented here is that no gluing between interfaces is necessary, rendering the production very reliable. As a biophysical application, we investigate the early time points of the assembly of vimentin intermediate filament proteins into higher-order structures. This weakly scattering protein system leads to high quality data in the new devices, thus opening up the way for numerous future applications."],["dc.identifier.doi","10.1039/C7LC00824D"],["dc.identifier.eissn","1473-0189"],["dc.identifier.issn","1473-0197"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86792"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.relation.eissn","1473-0189"],["dc.relation.issn","1473-0197"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","microfluidics"],["dc.title","Cyclic olefin copolymer as an X-ray compatible material for microfluidic devices"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Epple, Robert"],["dc.contributor.author","Krüger, Dennis"],["dc.contributor.author","Berulava, Tea"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Islam, Rezaul"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Fischer, André"],["dc.date.accessioned","2022-02-16T13:26:06Z"],["dc.date.available","2022-02-16T13:26:06Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1101/2020.11.27.401901"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/99925"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/138"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/92"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B01: Der Verteilung struktureller Lipide in synaptischen Membranen"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG A. Fischer (Epigenetics and Systems Medicine in Neurodegenerative Diseases)"],["dc.subject.gro","other"],["dc.title","The Coding And Small-Non-Coding Hippocampal Synaptic RNAome"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]