Köster, Sarah

[["dc.contributor.author","Schepers, Anna V."],["dc.contributor.author","Lorenz, Charlotta"],["dc.contributor.author","Köster, Sarah"],["dc.date.accessioned","2020-03-03T08:22:25Z"],["dc.date.available","2020-03-03T08:22:25Z"],["dc.date.issued","2019"],["dc.description.abstract","The cytoskeleton is formed by three types of filamentous proteins – microtubules, actin filaments, and intermediate filaments (IFs) – and enables cells to withstand external and internal forces. Vimentin is the most abundant IF in humans and has remarkable mechanical properties, such as high extensibility and stability. It is, however, unclear to which extent these properties are influenced by the electrostatic environment. Here, we study the mechanical properties of single vimentin filaments by employing optical trapping combined with microfluidics. Force-strain curves, recorded at varying ion concentrations and pH values, reveal that the mechanical properties of single vimentin IFs are influenced by direct (pH) and indirect (ionic) charge variations. By combination with Monte Carlo simulations, we connect these altered mechanics to electrostatic interactions of subunits within the filaments. We thus find possible mechanisms that allow cells to locally tune their stiffness without remodelling the entire cytoskeleton."],["dc.identifier.doi","10.1101/784025"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63070"],["dc.language.iso","en"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","membrane biophysics"],["dc.title","Tuning intermediate filament mechanics by indirect and direct charge variations"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4142"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","4154"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Komorowski, Karlo"],["dc.contributor.author","Sztucki, Michael"],["dc.contributor.author","Sharpnack, Lewis"],["dc.contributor.author","Brehm, Gerrit"],["dc.contributor.author","Köster, Sarah"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Schaeper, Jannis"],["dc.date.accessioned","2020-04-23T12:28:28Z"],["dc.date.available","2020-04-23T12:28:28Z"],["dc.date.issued","2020"],["dc.description.abstract","We have used time-resolved small-angle X-ray scattering (SAXS) to study the adhesion of lipid vesicles in the electrostatic strong-coupling regime induced by divalent ions. The bilayer structure and the interbilayer distance dw between adhered vesicles was studied for different DOPC:DOPS mixtures varying the surface charge density of the membrane, as well as for different divalent ions, such as Ca2+, Sr2+, and Zn2+. The results are in good agreement with the strong coupling theory predicting the adhesion state and the corresponding like-charge attraction based on ion-correlations. Using SAXS combined with the stopped-flow rapid mixing technique, we find that in highly charged bilayers the adhesion state is only of transient nature, and that the adhering vesicles subsequently transform to a phase of multilamellar vesicles, again with an inter-bilayer distance according to the theory of strong binding. Aside from the stopped-flow SAXS instrumentations used primarily for these results, we also evaluate microfluidic sample environments for vesicle SAXS in view of future extension of this work."],["dc.identifier.doi","10.1039/D0SM00259C"],["dc.identifier.pmid","32319505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64290"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/186"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/72"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A02: Bestimmung der Struktur synaptischer Organellen durch Röntgenbeugungs- und Bildgebungsverfahren"],["dc.relation","SFB 1286 | B02: Ein in vitro-Verfahren zum Verständnis der struktur-organisierenden Rolle des Vesikel-Clusters"],["dc.relation.eissn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Köster (Cellular Biophysics)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","CC BY-NC 3.0"],["dc.subject.gro","x-ray scattering"],["dc.subject.gro","membrane biophysics"],["dc.subject.gro","microfluidics"],["dc.title","Vesicle adhesion in the electrostatic strong-coupling regime studied by time-resolved small-angle X-ray scattering"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]