Bänsch, Svenja

[["dc.bibliographiccitation.journal","Frontiers in Plant Science"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Leidenfrost, Robert M."],["dc.contributor.author","Bänsch, Svenja"],["dc.contributor.author","Prudnikow, Lisa"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Westphal, Catrin"],["dc.contributor.author","Wünschiers, Röbbe"],["dc.date.accessioned","2021-04-14T08:26:59Z"],["dc.date.available","2021-04-14T08:26:59Z"],["dc.date.issued","2020"],["dc.description.abstract","Bumble bees are important crop pollinators and provide important pollination services to their respective ecosystems. Their pollen diet and thus food preferences can be characterized through nucleic acid sequence analysis. We present ITS2 amplicon sequence data from pollen collected by bumble bees. The pollen was collected from six different bumble bee colonies that were placed in independent agricultural landscapes. We compared next-generation (Illumina), i.e., short-read, and third-generation (Nanopore), i.e., MinION, sequencing techniques. MinION data were preprocessed using traditional and Nanopore specific tools for comparative analysis and were evaluated in comparison to short-read sequence data with conventional processing. Based on the results, the dietary diary of bumble bee in the studied landscapes can be identified. It is known that short reads generated by next-generation sequencers have the advantage of higher quality scores while Nanopore yields longer read lengths. We show that assignments to taxonomic units yield comparable results when querying against an ITS2-specific sequence database. Thus, lower sequence quality is compensated by longer read lengths. However, the Nanopore technology is improving in terms of data quality, much cheaper, and suitable for portable applications. With respect to the studied agricultural landscapes we found that bumble bees require higher plant diversity than only crops to fulfill their foraging requirements."],["dc.identifier.doi","10.3389/fpls.2020.00287"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17385"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82133"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-462X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Analyzing the Dietary Diary of Bumble Bee"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5003"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Molecular Ecology"],["dc.bibliographiccitation.lastpage","5018"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Bänsch, Svenja"],["dc.contributor.author","Tscharntke, Teja"],["dc.contributor.author","Wünschiers, Röbbe"],["dc.contributor.author","Netter, Leonie"],["dc.contributor.author","Brenig, Bertram"],["dc.contributor.author","Gabriel, Doreen"],["dc.contributor.author","Westphal, Catrin"],["dc.date.accessioned","2021-04-14T08:31:28Z"],["dc.date.available","2021-04-14T08:31:28Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract Worldwide pollinator declines lead to pollination deficits in crops and wild plants, and managed bees are frequently used to meet the increasing demand for pollination. However, their foraging can be affected by flower availability and colony size. We investigated how mass‐flowering oilseed rape (OSR) can influence the pollen resource use of small and large honey bee (Apis mellifera L.) and bumble bee (Bombus terrestris L.) colonies. Colonies were placed adjacent to strawberry fields along a gradient of OSR availability in the landscapes. We used ITS2 metabarcoding to identify the pollen richness based on ITS2 amplicon sequencing and microscopy for quantification of target pollen. Bumble bees collected pollen from more different plant genera than honey bees. In both species, strawberry pollen collection decreased with high OSR availability but was facilitated by increasing strawberry flower cover. Colony size had no effect. The relationship between next‐generation sequencing‐generated ITS2 amplicon reads and microscopic pollen counts was positive but pollen type‐specific. Bumble bees and, to a lesser degree, honey bees collected pollen from a wide variety of plants. Therefore, in order to support pollinators and associated pollination services, future conservation schemes should sustain and promote pollen plant richness in agricultural landscapes. Both bee species responded to the availability of flower resources in the landscape. Although honey bees collected slightly more strawberry pollen than bumble bees, both can be considered as crop pollinators. Metabarcoding could provide similar quantitative information to microscopy, taking into account the pollen types, but there remains high potential to improve the methodological weaknesses."],["dc.description.sponsorship","Deutsche Bundesstiftung Umwelt"],["dc.description.sponsorship","Sächsisches Staatsministerium für Wissenschaft und Kunst"],["dc.description.sponsorship","Bundesministerium für Bildung und Forschung"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.description.sponsorship","Open access funding enabled and organized by Projekt DEAL"],["dc.identifier.doi","10.1111/mec.15675"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83602"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1365-294X"],["dc.relation.issn","0962-1083"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Using ITS2 metabarcoding and microscopy to analyse shifts in pollen diets of honey bees and bumble bees along a mass‐flowering crop gradient"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]