Becker, Jürgen

[["dc.bibliographiccitation.artnumber","157"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cell & Bioscience"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Wilting, Jörg"],["dc.contributor.author","Becker, Jürgen"],["dc.date.accessioned","2022-09-19T07:17:59Z"],["dc.date.available","2022-09-19T07:17:59Z"],["dc.date.issued","2022-09-15"],["dc.date.updated","2022-09-18T03:12:12Z"],["dc.description.abstract","Abstract\r\n Almost 400 years after the (re)discovery of the lymphatic vascular system (LVS) by Gaspare Aselli (Asellius G. De lactibus, sive lacteis venis, quarto vasorum mesaraicorum genere, novo invento Gasparis Asellii Cremo. Dissertatio. (MDCXXIIX), Milan; 1628.), structure, function, development and evolution of this so-called ‘second’ vascular system are still enigmatic. Interest in the LVS was low because it was (and is) hardly visible, and its diseases are not as life-threatening as those of the blood vascular system. It is not uncommon for patients with lymphedema to be told that yes, they can live with it. Usually, the functions of the LVS are discussed in terms of fluid homeostasis, uptake of chylomicrons from the gut, and immune cell circulation. However, the broad molecular equipment of lymphatic endothelial cells suggests that they possess many more functions, which are also reflected in the pathophysiology of the system. With some specific exceptions, lymphatics develop in all organs. Although basic structure and function are the same regardless their position in the body wall or the internal organs, there are important site-specific characteristics. We discuss common structure and function of lymphatics; and point to important functions for hyaluronan turn-over, salt balance, coagulation, extracellular matrix production, adipose tissue development and potential appetite regulation, and the influence of hypoxia on the regulation of these functions. Differences with respect to the embryonic origin and molecular equipment between somatic and splanchnic lymphatics are discussed with a side-view on the phylogeny of the LVS. The functions of the lymphatic vasculature are much broader than generally thought, and lymphatic research will have many interesting and surprising aspects to offer in the future."],["dc.identifier.citation","Cell & Bioscience. 2022 Sep 15;12(1):157"],["dc.identifier.doi","10.1186/s13578-022-00898-0"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114251"],["dc.language.iso","en"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.subject","Initial lymphatics"],["dc.subject","Lymphatic collector"],["dc.subject","Lymphangiogenesis"],["dc.subject","Circulating endothelial precursor cells"],["dc.subject","Pacemaker cell"],["dc.subject","Smooth muscle cell origin"],["dc.subject","Sphingosine-1-phosphate"],["dc.subject","Melanocortin-2 receptor accessory protein-2"],["dc.title","The lymphatic vascular system: much more than just a sewer"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1057"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Cellular and Molecular Life Sciences"],["dc.bibliographiccitation.lastpage","1070"],["dc.bibliographiccitation.volume","75"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2020-12-10T14:07:53Z"],["dc.date.available","2020-12-10T14:07:53Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1007/s00018-017-2685-8"],["dc.identifier.eissn","1420-9071"],["dc.identifier.issn","1420-682X"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15539"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/70321"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","WNT signaling, the development of the sympathoadrenal–paraganglionic system and neuroblastoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Pregnancy and Childbirth"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Tchagou Tchangou, Gilles E."],["dc.contributor.author","Schmidt, Sonja"],["dc.contributor.author","Zelent, Christina"],["dc.contributor.author","Kahl, Fritz"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-04-14T08:25:15Z"],["dc.date.available","2021-04-14T08:25:15Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1186/s12884-020-03073-w"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17437"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81570"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","1471-2393"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Absence of lymphatic vessels in term placenta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Vascular Cell"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Becker, Jürgen"],["dc.contributor.author","Covelo-Fernandez, Ana"],["dc.contributor.author","von Bonin, Frederike"],["dc.contributor.author","Kube, Dieter"],["dc.contributor.author","Wilting, Jörg"],["dc.date.accessioned","2021-06-01T10:48:01Z"],["dc.date.available","2021-06-01T10:48:01Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1186/2045-824X-4-3"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7510"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85803"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-824X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Specific tumor-stroma interactions of EBV-positive Burkitt's lymphoma cells in the chick chorioallantoic membrane"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","339"],["dc.bibliographiccitation.journal","BMC Cancer"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Klingenberg, Marcel"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Eberth, Sonja"],["dc.contributor.author","Kube, Dieter"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T09:40:11Z"],["dc.date.available","2018-11-07T09:40:11Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Burkitt lymphoma (BL) is an aggressive malignancy that arises from B-cells and belongs to the group of Non-Hodgkin lymphomas (NHL). Due to the lack of appropriate in vivo models NHL research is mainly performed in vitro. Here, we studied the use of the chick chorioallantoic membrane (CAM) for the generation of human BL xenograft tumors, which we compared with known characteristics of the human disease. Methods: In order to generate experimental BL tumors, we inoculated human BL2B95 and BL2-GFP cells on the CAM. BL2B95 xenograft-tumors were grown for seven days and subsequently analyzed with transmission electron and immunofluorescence microscopy, as well as histological staining approaches. BL2-GFP cells were studied at regular intervals up to seven days, and their metastatic behavior was visualized with intravital immunofluorescence techniques. Results: Xenografted BL2B95 cells formed solid tumors in the CAM model with a Ki67-index greater than 90%, preservation of typical tumor markers (CD10, CD19, CD20), a 'starry sky' morphology, production of agyrophilic fibers in the stroma, formation of blood and lymphatic vessels and lymphogenic dissemination of BL2B95 to distant sites. We identified macrophages, lymphocytes and heterophilic granulocytes (chick homolog of neutrophils) as the most abundant immune cells in the experimental tumors. BL2-GFP cells could be traced in real-time during their distribution in the CAM, and the first signs for their dissemination were visible after 2-3 days. Conclusions: We show that xenografted BL2B95 cells generate tumors in the CAM with a high degree of cellular, molecular and proliferative concord with the human disease, supporting the application of the CAM model for NHL research with a focus on tumor-stroma interactions. Additionally we report that BL2-GFP cells, grafted on the CAM of ex ovo cultured chick embryos, provide a powerful tool to study lymphogenic dissemination in real-time."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [FOR942/12-1, GRK1034]"],["dc.identifier.doi","10.1186/1471-2407-14-339"],["dc.identifier.isi","000338141900001"],["dc.identifier.pmid","24884418"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10149"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33449"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2407"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","The chick chorioallantoic membrane as an in vivo xenograft model for Burkitt lymphoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0164964"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Hasselhof, Viktoria"],["dc.contributor.author","Sperling, Anastasia"],["dc.contributor.author","Buttler, Kerstin"],["dc.contributor.author","Strobel, Philipp"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Aung, Thiha"],["dc.contributor.author","Felmerer, Gunther"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2018-11-07T10:06:56Z"],["dc.date.available","2018-11-07T10:06:56Z"],["dc.date.issued","2016"],["dc.description.abstract","Millions of patients suffer from lymphedema worldwide. Supporting the contractility of lymphatic collectors is an attractive target for pharmacological therapy of lymphedema. However, lymphatics have mostly been studied in animals, while the cellular and molecular characteristics of human lymphatic collectors are largely unknown. We studied epifascial lymphatic collectors of the thigh, which were isolated for autologous transplantations. Our immunohistological studies identify additional markers for LECs (vimentin, CCBE1). We show and confirm differences between initial and collecting lymphatics concerning the markers ESAM1, D2-40 and LYVE-1. Our transmission electron microscopic studies reveal two types of smooth muscle cells (SMCs) in the media of the collectors with dark and light cytoplasm. We observed vasa vasorum in the media of the largest collectors, as well as interstitial Cajal-like cells, which are highly ramified cells with long processes, caveolae, and lacking a basal lamina. They are in close contact with SMCs, which possess multiple caveolae at the contact sites. Immunohistologically we identified such cells with antibodies against vimentin and PDGFR alpha, but not CD34 and cKIT. With Next Generation Sequencing we searched for highly expressed genes in the media of lymphatic collectors, and found therapeutic targets, suitable for acceleration of lymphatic contractility, such as neuropeptide Y receptors 1, and 5; tachykinin receptors 1, and 2; purinergic receptors P2RX1, and 6, P2RY12, 13, and 14; 5-hydroxytryptamine receptors HTR2B, and 3C; and adrenoceptors alpha(2A),(B),(C). Our studies represent the first comprehensive characterization of human epifascial lymphatic collectors, as a prerequisite for diagnosis and therapy."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.1371/journal.pone.0164964"],["dc.identifier.isi","000386204500086"],["dc.identifier.pmid","27764183"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13797"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39190"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Morphological and Molecular Characterization of Human Dermal Lymphatic Collectors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1013"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Cancers"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Becker, Juergen"],["dc.contributor.author","Wilting, Joerg"],["dc.date.accessioned","2019-07-29T15:17:10Z"],["dc.date.available","2019-07-29T15:17:10Z"],["dc.date.issued","2019"],["dc.description.abstract","The term WNT (wingless-type MMTV integration site family) signaling comprises a complex molecular pathway consisting of ligands, receptors, coreceptors, signal transducers and transcriptional modulators with crucial functions during embryonic development, including all aspects of proliferation, morphogenesis and differentiation. Its involvement in cancer biology is well documented. Even though WNT signaling has been divided into mainly three distinct branches in the past, increasing evidence shows that some molecular hubs can act in various branches by exchanging interaction partners. Here we discuss developmental and clinical aspects of WNT signaling in neuroblastoma (NB), an embryonic tumor with an extremely broad clinical spectrum, ranging from spontaneous differentiation to fatal outcome. We discuss implications of WNT molecules in NB onset, progression, and relapse due to chemoresistance. In the light of the still too high number of NB deaths, new pathways must be considered."],["dc.identifier.doi","10.3390/cancers11071013"],["dc.identifier.pmid","31331081"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16312"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62154"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2072-6694"],["dc.relation.issn","2072-6694"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","WNT Signaling in Neuroblastoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]