Großkopf, Birgit

[["dc.bibliographiccitation.firstpage","310"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C"],["dc.bibliographiccitation.lastpage","315"],["dc.bibliographiccitation.volume","35"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Ryban, L."],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Vondrakova, Maria"],["dc.contributor.author","Bauerova, Maria"],["dc.contributor.author","Fabis, M."],["dc.contributor.author","Chrenek, Peter"],["dc.date.accessioned","2018-11-07T09:08:08Z"],["dc.date.available","2018-11-07T09:08:08Z"],["dc.date.issued","2006"],["dc.description.abstract","The aim of this study was to investigate differences in compact bone tissue microstructure between New Zealand White transgenic rabbits with human blood clotting factor VIII gene and the non-transgenic ones. The transgene was under the transcriptional control of the whey acidic protein (WAP) promoter. Altogether, 42 femora were analysed. Specimens were prepared using standard histological equipment, producing thin sections approximately 80-100 mu m. Areas, perimeters, minimum and maximum diameters of vascular canals of primary osteons, Haversian canals and secondary osteons were measured. Our results indicate the basic structural pattern of the bone tissue was primary vascular longitudinal in both groups of rabbits. However, a fibrolamellar bone tissue was identified only in the transgenic rabbits. The measured variables of the vascular canals of the primary osteons, the Haversian canals and the secondary osteons were higher in transgenic rabbits in comparison with non-transgenic ones in most cases and the differences were often statistically significant (P < 0.01; P < 0.001). More significant differences were noticed in males (transgenic versus non-transgenic). However, it is not easy to find the real cause of the changes. Despite the fact that no hFVIII mRNA expression was found in the analysed bone of transgenic rabbits, we presume that the observed differences could be associated with transgenesis. In this article, we discuss possible associations between transgene integration as well as aneuploidy and/or observed microstructural changes in compact bone tissue."],["dc.identifier.doi","10.1111/j.1439-0264.2006.00690.x"],["dc.identifier.isi","000240401600007"],["dc.identifier.pmid","16968250"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25957"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0340-2096"],["dc.title","Comparative study of compact bone tissue microstructure between non-transgenic and transgenic rabbits with WAP-hFVIII gene construct"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","140"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","FOLIA BIOLOGICA"],["dc.bibliographiccitation.lastpage","144"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Chrenek, Peter"],["dc.contributor.author","Ryban, L."],["dc.contributor.author","Parkanyi, V."],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Vondrakova, Maria"],["dc.contributor.author","Bauerova, Maria"],["dc.date.accessioned","2018-11-07T08:38:59Z"],["dc.date.available","2018-11-07T08:38:59Z"],["dc.date.issued","2005"],["dc.description.abstract","Bone tissue microstructure of femur was investigated in transgenic New Zealand White rabbits with human factor VIII gene. Altogether 42 bones (24 from transgenic rabbits and 18 from non-transgenic ones) were analysed. Specimens were prepared using standard histological equipment, producing thin sections of approximately 80-100 mu m. For histomorphometrical analysis areas, perimeters, minimum and maximum diameters of osteons' vascular canals and of osteons were measured. We found out that the basic structural pattern of femoral bone tissue was primary vascular longitudinal in both groups of rabbits. However, a new type of the bone tissue - fibrolamellar - was identified only in the transgenic rabbits. The measured variables of the osteons' vascular canals were higher in transgenic individuals in comparison with the nontransgenie ones (except for maximal diameter) and the differences were statistically significant (P < 0.05; P < 0.01). We suppose that the observed differences could be associated with transgenesis. In an effort to explain these differences we compared the cytogenctic profile of bone marrow cells between transgenic and nontransgenic rabbits. A significantly higher rate of aneuploidy was observed in c-metaphase spreads of transgenic individuals as compared to non-transgenic ones (P < 0.001). Despite the fact that no hFVIII mRNA expression was found in the femur of transgenic rabbits, we discussed an association of transgene integration into the genome and microstructural changes in the bone. In any case, the results indicate that transgenesis can also produce changes in other tissues than in the target ones."],["dc.identifier.isi","000233052100004"],["dc.identifier.pmid","16285207"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18880"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Inst Molecular Genetics"],["dc.relation.issn","0015-5500"],["dc.title","Changes of femoral bone tissue microstructure in transgenic rabbits"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","150"],["dc.bibliographiccitation.journal","American Journal of Physical Anthropology"],["dc.bibliographiccitation.lastpage","151"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Chovancova, Hana"],["dc.contributor.author","Massanyi, Peter"],["dc.contributor.author","Chrenek, Peter"],["dc.date.accessioned","2018-11-07T11:19:19Z"],["dc.date.available","2018-11-07T11:19:19Z"],["dc.date.issued","2008"],["dc.identifier.isi","000253342000413"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55246"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.publisher.place","Hoboken"],["dc.relation.conference","77th Annual Meeting of the American-Association-of-Physical-Anthropologists"],["dc.relation.eventlocation","Columbus, OH"],["dc.relation.issn","0002-9483"],["dc.title","Effects of nickel and zinc administration on rabbit's compact bone: an experimental animal model."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Bone and Mineral Research"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Dammers, K."],["dc.contributor.author","Baucrova, M."],["dc.contributor.author","Chrenek, Peter"],["dc.date.accessioned","2018-11-07T11:11:34Z"],["dc.date.available","2018-11-07T11:11:34Z"],["dc.date.issued","2008"],["dc.format.extent","S159"],["dc.identifier.isi","000259411000568"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53465"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Bone & Mineral Res"],["dc.publisher.place","Washington"],["dc.relation.conference","30th Annual Meeting of the American-Society-for-Bone-and-Mineral-Research"],["dc.relation.eventlocation","Montreal, CANADA"],["dc.relation.issn","0884-0431"],["dc.title","Differences in Femoral Bone Structure Between Juvenile Non-transgenic and Transgenic Rabbits with the WAP-hFVIII Gene Construct"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","12"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","FOLIA BIOLOGICA"],["dc.bibliographiccitation.lastpage","17"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Smolarikova, V."],["dc.contributor.author","Bauerova, Maria"],["dc.contributor.author","Chrenek, Peter"],["dc.date.accessioned","2018-11-07T11:19:56Z"],["dc.date.available","2018-11-07T11:19:56Z"],["dc.date.issued","2008"],["dc.description.abstract","Differences in microscopic structure of the femur between 1-month-old transgenic rabbits carrying the hFVIII gene and non-transgenic rabbits were investigated. Bone microstructure was evaluated from the point of view of qualitative and quantitative histological characteristics. We identified fibrolamellar bone tissue only in the transgenic animals. Measured values for area, perimeter of the Haversian canals and minimum diameter of the primary osteons' vascular canals were higher in 1-month-old transgenic individuals (P < 0.05; P < 0.001). We also observed lower concentrations of Ca, P, K, solids, and total mineral content in femora of transgenic rabbits. A statistically significant difference was observed for the concentration of Ca (P < 0.05). Our results indicate evident changes in both qualitative and quantitative histological characteristics of the femur, which result especially in better blood supply and slightly reduced mineralization process in 1-month-old transgenic rabbits."],["dc.identifier.isi","000252876600003"],["dc.identifier.pmid","18226360"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55407"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Charles Univ Prague, First Faculty Medicine"],["dc.relation.issn","0015-5500"],["dc.title","Femoral bone microstructure in 1-month-old non-transgenic versus transgenic rabbits with the WAP-hFVIII gene construct"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]