Großkopf, Birgit

[["dc.bibliographiccitation.artnumber","8"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Biological Research-Thessaloniki"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Sarocka, Anna"],["dc.contributor.author","Babosova, Ramona"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Kapusta, Edyta"],["dc.contributor.author","Goc, Zofia"],["dc.contributor.author","Formicki, Grzegorz"],["dc.contributor.author","Omelka, Radoslav"],["dc.date.accessioned","2019-07-09T11:45:30Z"],["dc.date.available","2019-07-09T11:45:30Z"],["dc.date.issued","2018"],["dc.description.abstract","Background: Alcohol is one of the most commonly consumed neurotoxins by humans. Its negative effect on bone health is known for a long time. However, its impact on qualitative and quantitative 2D characteristics of the compact bone is still unclear. Therefore, the aim of this study was to investigate in detail the effects of subchronic alcohol exposure on compact and trabecular bone tissues microstructure of laboratory mice using 2D and 3D imaging methods. Ten clinically healthy 12 weeks-old mice (males) were randomly divided into two groups. Animals from experimental group (group E; n = 5) drank a solution composed of 15% ethanol and water (1.7 g 100% ethanol kg-1 b.w. per day) for 8 weeks, while those from control group (group C; n = 5) drank only water. Results: Subchronic exposure to alcohol leads to several changes in qualitative 2D characteristics of the compact bone such as the presence of primary vascular radial bone tissue in pars anterior of endosteal border and a higher number of resorption lacunae (five times more) in the middle part of substantia compacta. Morphometrical 2D evaluations of the compact bone showed significantly increased sizes of primary osteons' vascular canals (p < 0.05) in mice from the experimental group (E group). Sizes of Haversian canals and secondary osteons were not affected by alcohol consumption. In mice from the E group, significantly lower values for relative bone volume and bone mineral density of the compact bone were observed. In the trabecular bone, decreased values for bone volume, trabecular number, trabecular thickness and bone surface (p < 0.05) were documented. Conclusions: Alcohol decreased not only bone volume and density of the compact bone, but it also reduced trabecular bone volume and leads to trabecular thinning. It caused vasodilation of primary osteons' vascular canals and increased porosity in the compact bone."],["dc.identifier.doi","10.1186/s40709-018-0079-1"],["dc.identifier.pmid","29876325"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15229"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59242"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","BioMed Central"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Changes in the microstructure of compact and trabecular bone tissues of mice subchronically exposed to alcohol"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Pharmacology and Toxicology"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Sarocka, Anna"],["dc.contributor.author","Kovacova, Veronika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Kapusta, Edyta"],["dc.contributor.author","Goc, Zofia"],["dc.contributor.author","Formicki, Grzegorz"],["dc.contributor.author","Martiniakova, Monika"],["dc.date.accessioned","2019-07-09T11:51:58Z"],["dc.date.available","2019-07-09T11:51:58Z"],["dc.date.issued","2019"],["dc.description.abstract","Abstract Background This study aimed to examine femoral bone microstructure of mice after single and simultaneous administration to acrylamide and ethanol since both substances are often consumed separately and/or together by humans. Interactive effects of these toxins were analysed after one remodeling cycle. Methods Twenty clinically healthy adult mice were randomly divided into four groups following 2 weeks administration of toxins: A group - mice were fed with acrylamide (40 mg/kg bw); E group - mice were ethanol-fed (15% ethanol); AE group - mice were simultaneously fed with both toxins, and a C group – control (without acrylamide and/or ethanol supplementation). Generally, 2D and 3D imaging methods were used to determine cortical and trabecular bone tissues microstructure. Biochemical analyses of plasma parameters were also realized using commercially available ELISA tests and spectrophotometrically. Results Single and simultaneous exposure to acrylamide and ethanol affected only cortical bone microstructure. No significant changes in trabecular bone morphometry were detected among all groups. In mice from the A group, increased endocortical remodeling associated with a higher level of serum calcium and vasoconstriction of primary osteon’s vascular canals (POVC) were identified. On the contrary, increased cortical porosity consistent with a decreased relative bone volume, bone mineral density (BMD) and lower levels of alkaline phosphatase (ALP), glutathione (GSH), calcium in plasma and also with vasodilation of POVC were observed in the E group. In the AE group, the highest density of secondary osteons associated with a lower BMD and decreased levels of ALP, GSH were documented. The parameters of POVC and Haversian canals approximated to the C group. In addition, single and simultaneous exposure to both toxins caused liver disease consistent with a higher values of alanine aminotransferase (ALT), aspartate aminotransferase (AST) in plasma of all experimental groups. Conclusions Single administration to acrylamide and ethanol had negative effects on cortical bone structure of mice after one remodeling cycle. However, we identified possible antagonistic impact of these toxins on the structure of the cortical bone."],["dc.identifier.doi","10.1186/s40360-019-0317-7"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16252"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60055"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","BioMed Central"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Single and simultaneous effects of acrylamide and ethanol on bone microstructure of mice after one remodeling cycle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Acta veterinaria Scandinavica"],["dc.bibliographiccitation.lastpage","6"],["dc.bibliographiccitation.volume","50"],["dc.contributor.author","Martiniaková, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Sirotkin, Alexander V."],["dc.contributor.author","Chrenek, Peter"],["dc.date.accessioned","2019-07-10T08:12:57Z"],["dc.date.available","2019-07-10T08:12:57Z"],["dc.date.issued","2008"],["dc.description.abstract","Background: While gross morphological changes in the skeleton between males and females are well know, differences between sexes in the histomorphology are less known. It is important to have knowledge on the bone structure of rabbits, as this is a widely used species in biomedical research. A study was performed to evaluate the association between sex and the compact bone morphology of the femoral diaphysis in juvenile rabbits.Methods:Seventeen clinically healthy 23 month-old rabbits (9 females, 8 males) were included in the study. The rabbits were euthanized and the right femur was sampled for analysis. 7080 microns thick bone sections of the femoral diaphysis were prepared using standard histological equipment. The qualitative histological characteristics were determined according to internationally accepted classification systems while the quantitative parameters were assessed using the software Scion Image. Areas, perimeters, minimum and maximum diameters of primary osteons' vascular canals, Haversian canals and secondary osteons were measured. Additionally, blood plasma concentrations of progesterone, corticosterone, IGF-I, testosterone and estradiol were analyzed.Results: Qualitative histological characteristics were similar for both sexes. However, variations of certain quantitative histological characteristics were identified. Measured parameters of the primary osteons' vascular canals were higher in males than for females. On the other hand, females had significant higher values of secondary osteons parameters. Differences in Haversian canals parameters were only significant for minimum diameter. Conclusion: The study demonstrated that quantitative histological characteristics of compact bone tissue of the femoral diaphysis in juvenile rabbits were sex dependent. The variations may be associated with different growth and modeling of the femur through influence by sex-specific steroids, mechanical loads, genetic factors and a multitude of other sources. The results can be applied in experimental studies focusing on comparison of the skeletal biology of the sexes."],["dc.identifier.fs","492195"],["dc.identifier.ppn","57562440X"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4339"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61084"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1751-0147"],["dc.relation.orgunit","Fakultät für Biologie und Psychologie"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","590"],["dc.title","Sex-related variation in compact bone microstructure of the femoral diaphysis in juvenile rabbits"],["dc.title.alternative","Research"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","43"],["dc.bibliographiccitation.journal","ACTA VETERINARIA SCANDINAVICA"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Babosova, Ramona"],["dc.contributor.author","Duranova, Hana"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Kovacova, Veronika"],["dc.contributor.author","Adamkovicova, Maria"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Capcarova, Marcela"],["dc.contributor.author","Martiniakova, Monika"],["dc.date.accessioned","2018-11-07T10:12:34Z"],["dc.date.available","2018-11-07T10:12:34Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: Quercetin is one of the best known flavonoids being present in a variety of fruits and vegetables. It has cardioprotective, anticarcinogenic, antioxidant, anti-inflammatory and antiapoptotic properties. Some studies suggest that quercetin has protective effects on bone. However, its influence on qualitative and quantitative histological characteristics of compact bone is still unknown. In our study, 12 clinically healthy five-month-old female rabbits were divided into four groups of three animals each. Quercetin was applied intramuscularly in various concentrations; 10 mu g/kg body weight (bw) in the E1 group, 100 mu g/kg bw in the E2 group, and 1000 mu g/kg bw in the E3 group for 90 days, 3 times per week. Three rabbits without exposure to quercetin served as a control (C) group. Differences in femoral bone microstructure among groups were evaluated. Results: Qualitative histological characteristics of compact bone differed between rabbits from the E1 and E2 groups. Primary vascular longitudinal bone tissue was not found in some areas near the endosteal surface due to increased endocortical bone resorption. In addition, periosteal border of rabbits from the E1 group was composed of a thicker layer of primary vascular longitudinal bone tissue than in the other groups. In all groups of rabbits administered quercetin, a lower density of secondary osteons was observed. Histomorphometrical evaluations showed significantly decreased sizes of the primary osteons' vascular canals in individuals from the E1 and E2 groups. Secondary osteons were significantly smaller in rabbits from the E1, E2, E3 groups when compared to the C group. Cortical bone thickness was significantly increased in females from the E1 and E2 groups. Conclusions: The results indicate that quercetin has not only a positive dose-response on qualitative and quantitative histological characteristics of the compact bone of female rabbits as it would be expected."],["dc.identifier.doi","10.1186/s13028-016-0225-4"],["dc.identifier.isi","000379248600001"],["dc.identifier.pmid","27357122"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13383"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40262"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1751-0147"],["dc.relation.issn","0044-605X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Structural changes in femoral bone microstructure of female rabbits after intramuscular administration of quercetin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","58"],["dc.bibliographiccitation.journal","ACTA VETERINARIA SCANDINAVICA"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Jancova, Alena"],["dc.date.accessioned","2018-11-07T08:37:02Z"],["dc.date.available","2018-11-07T08:37:02Z"],["dc.date.issued","2010"],["dc.description.abstract","Background: Free-living wild rodents are often used as zoomonitors of environmental contamination. In the present study, accumulation of cadmium (Cd), copper (Cu), iron (Fe), and zinc (Zn) in critical organs of yellow-necked mice (Apodemus flavicollis) and bank voles (Myodes glareolus) trapped in a polluted area in Novaky, Slovakia was investigated. Methods: Yellow-necked mice (n = 8) and bank voles (n = 10) were collected using standard theriological methods for wood ecosystems. All animals were adult males in good physical condition. The concentrations of Cd, Cu, Fe, and Zn in the liver, kidney, and bone were determined by atomic absorption spectrophotometry. Results: The highest concentrations of Cd and Zn were found in the bone of both species while Cu and Fe accumulated mainly in kidney or liver. Significant higher concentrations of Cd and Cu were detected in the liver of bank voles than in yellow-necked mice. Similar significant higher levels of Cd and Zn were found in the bone of bank voles. In contrast, significant higher concentrations of Cu and Fe were present in the kidney of yellow-necked mice. Conclusions: In the yellow-necked mouse and bank vole, bone seems to accumulate Cd and Zn following prolonged exposure. On the contrary, kidney and liver store Cu and Fe after a long-term environmental exposure. In the present study, bank voles seemed to be more heavy metal loaded zoomonitors than yellow-necked mice."],["dc.description.sponsorship","Ministry of Education, Slovakia [KEGA 3/7338/09]"],["dc.identifier.doi","10.1186/1751-0147-52-58"],["dc.identifier.isi","284276100001"],["dc.identifier.pmid","21054852"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5725"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18439"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","0044-605X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Yellow-necked mice (Apodemus flavicollis) and bank voles (Myodes glareolus) as zoomonitors of environmental contamination at a polluted area in Slovakia"],["dc.title.original","5725"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","64"],["dc.bibliographiccitation.journal","ACTA VETERINARIA SCANDINAVICA"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Duranova, Hana"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Bobonova, Ivana"],["dc.contributor.author","Toman, Robert"],["dc.date.accessioned","2018-11-07T09:35:03Z"],["dc.date.available","2018-11-07T09:35:03Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Chronic exposure to cadmium (Cd), even at low concentrations, has an adverse impact on the skeletal system. Histologically, primary and secondary osteons as basic structural elements of compact bone can also be affected by several toxicants leading to changes in bone vascularization and mechanical properties of the bone. The current study was designed to investigate the effect of subchronic peroral exposure to Cd on femoral bone structure including histomorphometry of the osteons in adult male rats. In our study, 20 one-month-old male Wistar rats were randomly divided into two experimental groups. In the first group, young males received a drinking water containing 30 mg of CdCl2/L, for 90 days. Ten one-month-old males without Cd intoxication served as a control group. After 90 days of daily peroral exposure, body weight, femoral weight, femoral length, cortical bone thickness and histological structure of the femora were analysed. Results: We found that subchronic peroral application of Cd had no significant effect on body weight, femoral length and cortical bone thickness in adult rats. On the other hand, femoral weight was significantly increased (P < 0.05) in Cd-intoxicated rats. These rats also displayed different microstructure in the middle part of the compact bone where vascular canals expanded into central area of substantia compacta and supplied primary and secondary osteons. Additionally, a few resorption lacunae which are connected with an early stage of osteoporosis were identified in these individuals. Histomorphometrical evaluations showed that all variables (area, perimeter, maximum and minimum diameter) of the primary osteons' vascular canals, Haversian canals and secondary osteons were significantly decreased (P < 0.05) in the Cd group rats. This fact points to alterations in bone vascularization. Conclusions: Subchronic peroral exposure to Cd significantly influences femoral weight and histological structure of compact bone in adult male rats. It induces an early stage of osteoporosis and causes reduced bone vascularization. Histomorphometrical changes of primary and secondary osteons allow for the conclusion that the bone mechanical properties could be weakened in the Cd group rats. The current study significantly expands the knowledge on damaging action of Cd on the bone."],["dc.identifier.doi","10.1186/s13028-014-0064-0"],["dc.identifier.isi","000342596200001"],["dc.identifier.pmid","25279860"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10959"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32309"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1751-0147"],["dc.relation.issn","0044-605X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Changes in compact bone microstructure of rats subchronically exposed to cadmium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","49"],["dc.bibliographiccitation.journal","ACTA VETERINARIA SCANDINAVICA"],["dc.bibliographiccitation.volume","53"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Chovancova, Hana"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Toman, Robert"],["dc.date.accessioned","2018-11-07T08:53:03Z"],["dc.date.available","2018-11-07T08:53:03Z"],["dc.date.issued","2011"],["dc.description.abstract","Background: Exposure to cadmium (Cd) is considered a risk factor for various bone diseases in humans and experimental animals. This study investigated the acute effects of Cd on femoral bone structure of adult male rats after a single intraperitoneal administration. Methods: Ten 4-month-old male Wistar rats were injected intraperitoneally with a single dose of 2 mg CdCl(2)/kg body weight and killed 36 h after the Cd had been injected. Ten 4-month-old males served as a control group. Differences in body weight, femoral weight, femoral length and histological structure of the femur were evaluated between the two groups of rats. The unpaired Student's t-test was used for establishment of statistical significance. Results: A single intraperitoneal administration of Cd had no significant effect on the body weight, femoral weight or femoral length. On the other hand, histological changes were significant. Rats exposed to Cd had significantly higher values of area, perimeter, maximum and minimum diameters of the primary osteons' vascular canals and Haversian canals. In contrast, a significant decrease in all variables of the secondary osteons was observed in these rats. Conclusions: The results indicate that, as expected, a single intraperitoneal administration of 2 mg CdCl(2)/kg body weight had no impact on macroscopic structure of rat's femora; however, it affected the size of vascular canals of primary osteons, Haversian canals, and secondary osteons."],["dc.identifier.doi","10.1186/1751-0147-53-49"],["dc.identifier.isi","000295218500001"],["dc.identifier.pmid","21884588"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6944"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22315"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1751-0147"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Effects of a single intraperitoneal administration of cadmium on femoral bone structure in male rats"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","174"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Medical Genetics"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Mondockova, Vladimira"],["dc.contributor.author","Adamkovicova, Maria"],["dc.contributor.author","Lukacova, Martina"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Babosova, Ramona"],["dc.contributor.author","Galbavy, Drahomir"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.date.accessioned","2019-07-09T11:45:53Z"],["dc.date.available","2019-07-09T11:45:53Z"],["dc.date.issued","2018"],["dc.description.abstract","Abstract Background The study investigated the associations of rs9340799:A > G (XbaI) and rs2234693:T > C (PvuII) polymorphisms in the estrogen receptor 1 gene (ESR1) with femoral neck (BMD-FN) and lumbar spine bone mineral density (BMD-LS), biochemical markers of bone turnover, calcium and phosphate levels, fracture prevalence, and a response to two types of anti-osteoporotic therapy in postmenopausal women from southern Slovakia. Methods We analysed 343 postmenopausal Slovak women (62.40 ± 0.46 years). The influence of rs9340799 (AA vs. AG + GG) and rs2234693 (TT vs. TC + CC) genotypes on BMD and biochemical markers was evaluated by covariance analysis adjusted for age and BMI. Binary logistic regression was used to evaluate the genotype effect on fracture prevalence. Pharmacogenetic part of the study included women who received a regular therapy of HT (17ß estradiol with progesterone; 1 mg/day for both; N = 76) or SERMs/raloxifene (60 mg/day; N = 64) during 48 months. The genotype-based BMD change was assessed by variance analysis for repeated measurements. Results Women with AA genotype of rs9340799 had higher BMD-FN (+ 0.12 ± 0.57 of T-score) and BMD-LS (+ 0.17 ± 0.08 of T-score) in comparison with AG + GG. The rs2234693 polymorphism did not affect any of the monitored parameters. No effect of any ESR1 polymorphisms was found on fracture prevalence. Both types of anti-osteoporotic therapy had a positive effect on BMD improvement in FN and LS sites. Considering the effect of the ESR1 gene within the HT, the subjects with rs9340799/AA genotype showed worse response than those with GG genotype (− 0.26 ± 0.10 of BMD-FN T-score; − 0.35 ± 0.10 of BMD-LS T-score) and also with AG genotype (− 0.22 ± 0.08 of BMD-LS T-score). The rs2234693/TT genotype responded poorer in BMD-LS in comparison with TC (− 0.22 ± 0.08 of T-score) and CC (− 0.35 ± 0.09 of T-score). The effect of the ESR1 gene on raloxifene therapy was reported only in BMD-LS. Subjects with rs9340799/AA genotype had a − 0.30 ± 0.11 of T-score worse response compared to AG genotype. The rs2234693/TT genotype showed − 0.39 ± 0.11 and − 0.46 ± 0.15 lower T-scores in comparison with TC and CC genotypes, respectively. Conclusions The rs9340799 polymorphism may contribute to decreased BMD in postmenopausal women from southern Slovakia; however, this is not related to higher fracture prevalence. Concurrently, both polymorphisms affected a response to analysed anti-osteoporotic therapies."],["dc.identifier.doi","10.1186/s12881-018-0684-8"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15336"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59329"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","BioMed Central"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The estrogen receptor 1 gene affects bone mineral density and osteoporosis treatment efficiency in Slovak postmenopausal women"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","50"],["dc.bibliographiccitation.journal","ACTA VETERINARIA SCANDINAVICA"],["dc.bibliographiccitation.volume","57"],["dc.contributor.author","Duranova, Hana"],["dc.contributor.author","Kovacova, Veronika"],["dc.contributor.author","Babosova, Ramona"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Adamkovicova, Maria"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Capcarova, Marcela"],["dc.contributor.author","Martiniakova, Monika"],["dc.date.accessioned","2018-11-07T09:51:47Z"],["dc.date.available","2018-11-07T09:51:47Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: Patulin, a toxic mold metabolite, has been found as natural contaminant of processed fruits, most notably apples, apple juices and other apple-based products. A number of adverse health effects in humans and animals are associated with patulin intoxication. The current study was performed to analyse possible toxic effects of patulin on femoral bone microstructure in adult rabbits in detail. Fourteen clinically healthy four-month-old rabbits of both sexes (6 males and 8 females) were included in the study. Animals from the experimental groups (group E male, n = 3; group E female, n = 4) were injected intramuscularly with patulin at dose 10 mu g/kg body weight two times a week for 28 days. The dose of patulin was estimated based on the maximum permitted level of patulin for apple products intended for infants and young children. Three males and four females without patulin administration served as controls (groups C male and C female). Cortical bone thickness and qualitative and quantitative histological characteristics of compact bone tissue were investigated. Results: Intramuscular applications of patulin significantly increased the thickness of cortical bone in both sexes of rabbits. In patulin-exposed males, an absence of primary vascular longitudinal bone tissue near the endosteal border was observed, which could be associated with intensive bone remodeling. Femoral diaphyses of females displayed a lower number of secondary osteons in the middle part of the substantia compacta, and occurrence of the osteons near the periosteum. This could indicate alterations in bone turnover. Histomorphometrical evaluations showed significantly increased sizes of the primary osteons' vascular canals (P < 0.05) in males exposed to patulin possibly due to mycotoxin-induced increased levels of testosterone. Conclusions: This study demonstrates significant impact of intramuscular application of patulin on bone microstructure in adult rabbits. Moreover, we have found that the effects of patulin on qualitative and quantitative histological characteristics of compact bone are sex-dependent."],["dc.identifier.doi","10.1186/s13028-015-0140-0"],["dc.identifier.isi","000360601500001"],["dc.identifier.pmid","26337444"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12334"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/35982"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1751-0147"],["dc.relation.issn","0044-605X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Sex-related variations in bone microstructure of rabbits intramuscularly exposed to patulin"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","52"],["dc.bibliographiccitation.journal","ACTA VETERINARIA SCANDINAVICA"],["dc.bibliographiccitation.volume","51"],["dc.contributor.author","Martiniakova, Monika"],["dc.contributor.author","Omelka, Radoslav"],["dc.contributor.author","Grosskopf, Birgit"],["dc.contributor.author","Chovancova, Hana"],["dc.contributor.author","Massanyi, Peter"],["dc.contributor.author","Chrenek, Peter"],["dc.date.accessioned","2018-11-07T11:21:04Z"],["dc.date.available","2018-11-07T11:21:04Z"],["dc.date.issued","2009"],["dc.description.abstract","Background: Nickel (Ni) and zinc (Zn) are trace elements present at low concentrations in agroecosystems. Nickel, however, may have toxic effects on living organisms and is often considered as a contaminant. This study reports the effect of peroral administrated Ni or a combination of Ni and Zn on femoral bone structure in rabbits. Methods: One month-old female rabbits were divided into three groups of five animals each. Group 1 rabbits were fed a granular feed mixture with addition of 35 g NiCl(2) per 100 kg of mixture for 90 days. In group 2, animals were fed a mixture containing 35 g NiCl(2) and 30 g ZnCl(2) per 100 kg of mixture. Group 3 without administration of additional Ni or Zn served as control. After the 90-day experimental period, femoral length, femoral weight and histological structure of the femur were analyzed and compared. Results: The results did not indicate a statistically significant difference in either femoral length or weight between the two experimental groups and the control group. Also, differences in qualitative histological characteristics of the femora among rabbits from the three groups were absent, except for a fewer number of secondary osteons found in the animals of groups 1 and 2. However, values for vascular canal parameters of primary osteons were significantly lower in group 1 than in the control one. Peroral administration of a combination of Ni and Zn ( group 2) led to a significant decreased size of the secondary osteons. Conclusions: The study indicates that dietary supplementation of Ni (35 g NiCl(2) per 100 kg of feed mixture) and Ni-Zn combination (35 g NiCl(2) and 30 g ZnCl(2) per 100 kg of the mixture) affects the microstructure of compact bone tissue in young rabbits."],["dc.description.sponsorship","Ministry of Education, Slovakia [KEGA 3/7338/09]"],["dc.identifier.doi","10.1186/1751-0147-51-52"],["dc.identifier.isi","000273287900001"],["dc.identifier.pmid","20003522"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5789"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55687"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","0044-605X"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Effects of dietary supplementation of nickel and nickel-zinc on femoral bone structure in rabbits"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]