Fine, Tamir

[["dc.bibliographiccitation.firstpage","2287"],["dc.bibliographiccitation.issue","13-14"],["dc.bibliographiccitation.journal","Journal of Adhesion Science and Technology"],["dc.bibliographiccitation.lastpage","2300"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Lorenz, Bärbel"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Fine, Tamir"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Wegener, Joachim"],["dc.date.accessioned","2017-09-07T11:46:42Z"],["dc.date.available","2017-09-07T11:46:42Z"],["dc.date.issued","2010"],["dc.description.abstract","The adhesion of MDCK II cells to porous and non-porous silicon substrates has been investigated by means of fluorescence and atomic force microscopy. The MDCK II cell density and the average height of the cells were increased on porous silicon substrates with regular 1.2 mu m pores as compared to flat, non-porous surfaces. In addition, we found a substantially reduced actin cytoskeleton within confluent cells cultured on the macroporous substrate compared to flat surfaces. The perturbation of the cytoskeleton relates to a significantly reduced expression of integrins on the porous area. The loss of stress fibers and cortical actin is accompanied by a dramatically reduced Young's modulus of 0.15 kPa compared to 6 kPa on flat surfaces as revealed by site-specific force-indentation experiments. (C) Koninklijke Brill NV, Leiden, 2010"],["dc.identifier.doi","10.1163/016942410X508028"],["dc.identifier.gro","3142996"],["dc.identifier.isi","000284152300013"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/462"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0169-4243"],["dc.title","Cell Adhesion to Ordered Pores: Consequences for Cellular Elasticity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3262"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","3265"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Fine, Tamir"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Rommel, Christina"],["dc.contributor.author","Wegener, Joachim"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2017-09-07T11:47:36Z"],["dc.date.available","2017-09-07T11:47:36Z"],["dc.date.issued","2009"],["dc.description.abstract","Apical cell membranes obtained from polar epithelial MDCK II cells were prepared on a highly ordered porous substrate, which allows local elastic mapping by force indentation curves."],["dc.identifier.doi","10.1039/b901714c"],["dc.identifier.gro","3143184"],["dc.identifier.isi","000269062900012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/670"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1744-683X"],["dc.title","Elasticity mapping of apical cell membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11490"],["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Soft Matter"],["dc.bibliographiccitation.lastpage","11502"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Fine, Tamir"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:29:30Z"],["dc.date.available","2018-11-07T09:29:30Z"],["dc.date.issued","2013"],["dc.description.abstract","Epithelial cells usually form a dense continuous cobblestone-like sheet that is frequently exposed to a variety of mechanical challenges encompassing osmotic stress and external forces. The response to external forces was investigated and the question of how individual polar epithelial cells organized in confluent monolayers respond to pharmaceutical stimuli targeting the key players of cellular mechanics was answered. In particular, we ask how epithelial cells respond to changes in cortical and membrane tension by surface area regulation if challenged by diverse chemical and mechanical cues. Here, a tension-based model is used that allows capturing the relevant modes of cell deformation. Together with independent measurements of membrane tension, cortical tension and excess surface area of confluent MDCK II cells it is possible to draw a mechanistic picture of how confluent cells respond to mechanical stimuli in general. Changes in tension are provoked by external stimuli directed towards the contractile actomyosin cortex (cytochalasin D, blebbistatin), and changes in the excess surface area are produced by cholesterol extraction (methyl-beta-cyclodextrin) or inhibition of dynamin (dynasore). A combination of AFM-indentation experiments with membrane-tether pulling at the same position allowed us to simultaneously monitor changes in membrane tension, cortical tension and excess surface area. Generally, we observed that removing or producing excess surface area of the plasma membrane readily adjusts membrane tension that is pivotal for the mechanical response of confluent cells. We found that isolated apical membranes from confluent MDCK II monolayers display similar mechanical properties as the apical side of living MDCK II cells in a confluent monolayer confirming that membrane mechanics in conjunction with cytoskeletal adhesion dominates the elastic response of confluent epithelial cells at large strain."],["dc.identifier.doi","10.1039/c3sm51610e"],["dc.identifier.isi","000327500200008"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10829"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31051"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1744-6848"],["dc.relation.issn","1744-683X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Elastic properties of cells in the context of confluent cell monolayers: impact of tension and surface area regulation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","832"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Small"],["dc.bibliographiccitation.lastpage","838"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Lorenz, Bärbel"],["dc.contributor.author","Mey, Ingo"],["dc.contributor.author","Steltenkamp, Siegfried"],["dc.contributor.author","Fine, Tamir"],["dc.contributor.author","Rommel, Christina"],["dc.contributor.author","Müller, Martin Michael"],["dc.contributor.author","Maiwald, Alexander"],["dc.contributor.author","Wegener, Joachim"],["dc.contributor.author","Steinem, Claudia"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2017-09-07T11:47:29Z"],["dc.date.available","2017-09-07T11:47:29Z"],["dc.date.issued","2009"],["dc.description.abstract","The mechanics of cellular membranes are governed by a non-equilibrium composite framework consisting of the semiflexible filamentous cytoskeleton and extracellular matrix proteins linked to the lipid bilayer. While elasticity information of plasma membranes has mainly been obtained from whole cell analysis, techniques that allow addressing local mechanical properties of cell membranes are desirable to learn how their lipid and protein composition is reflected in the elastic behavior on local length scales. Introduced here is an approach based on basolateral membranes of polar epithelial Madin-Darby canine kidney (MDCK) H cells, prepared on a highly ordered porous substrate that allows elastic mapping on a submicrometer-length scale. A strong correlation between the density of actin filaments and the measured membrane elasticity is found. Spatially resolved indentation experiments carried out with atomic force and fluorescence microscope permit relation of the supramolecular structure to the elasticity of cellular membranes. It is shown that the elastic response of the pore spanning cell membranes is governed by local bending modules rather than lateral tension."],["dc.identifier.doi","10.1002/smll.200800930"],["dc.identifier.gro","3143128"],["dc.identifier.isi","000265171600011"],["dc.identifier.pmid","19242949"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/608"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1613-6810"],["dc.title","Elasticity Mapping of Pore-Suspending Native Cell Membranes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]