Kaever, Alexander

[["dc.bibliographiccitation.firstpage","823"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","New Phytologist"],["dc.bibliographiccitation.lastpage","837"],["dc.bibliographiccitation.volume","202"],["dc.contributor.author","König, Stefanie"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Thurow, Corinna"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Gatz, Christiane"],["dc.contributor.author","Polle, Andrea"],["dc.contributor.author","Feußner, Ivo"],["dc.date.accessioned","2017-09-07T11:50:45Z"],["dc.date.available","2017-09-07T11:50:45Z"],["dc.date.issued","2014"],["dc.description.abstract","Summary - Verticillium longisporum is a soil‐borne vascular pathogen causing economic loss in rape. Using the model plant Arabidopsis this study analyzed metabolic changes upon fungal infection in order to identify possible defense strategies of Brassicaceae against this fungus. - Metabolite fingerprinting identified infection‐induced metabolites derived from the phenylpropanoid pathway. Targeted analysis confirmed the accumulation of sinapoyl glucosides, coniferin, syringin and lignans in leaves from early stages of infection on. At later stages, the amounts of amino acids increased. - To test the contribution of the phenylpropanoid pathway, mutants in the pathway were analyzed. The sinapate‐deficient mutant fah1‐2 showed stronger infection symptoms than wild‐type plants, which is most likely due to the lack of sinapoyl esters. Moreover, the coniferin accumulating transgenic plant UGT72E2‐OE was less susceptible. Consistently, sinapoyl glucose, coniferyl alcohol and coniferin inhibited fungal growth and melanization in vitro, whereas sinapyl alcohol and syringin did not. The amount of lignin was not significantly altered supporting the notion that soluble derivatives of the phenylpropanoid pathway contribute to defense. - These data show that soluble phenylpropanoids are important for the defense response of Arabidopsis against V. longisporum and that metabolite fingerprinting is a valuable tool to identify infection‐relevant metabolic markers."],["dc.identifier.doi","10.1111/nph.12709"],["dc.identifier.gro","3147731"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5128"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.notes.submitter","chake"],["dc.relation.issn","0028-646X"],["dc.title","Soluble phenylpropanoids are involved in the defense response of Arabidopsis against Verticillium longisporum"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1086"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","New Phytologist"],["dc.bibliographiccitation.lastpage","1097"],["dc.bibliographiccitation.volume","196"],["dc.contributor.author","Koenig, Stefanie"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Schwarz, Marnie"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Iven, Tim"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Ternes, Philipp"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Lipka, Volker"],["dc.contributor.author","Feussner, Ivo"],["dc.date.accessioned","2018-11-07T09:03:07Z"],["dc.date.available","2018-11-07T09:03:07Z"],["dc.date.issued","2012"],["dc.description.abstract","In Arabidopsis, the fatty acid moiety of sphingolipids is mainly alpha-hydroxylated. The consequences of a reduction in this modification were analysed. Mutants of both Fatty Acid Hydroxylase genes (AtFAH1 and AtFAH2) were analysed for sphingolipid profiles. To elucidate further consequences of the mutations, metabolic analyses were performed and the influence on pathogen defence was determined. Ceramide and glucosylceramide profiles of double-mutant plants showed a reduction in sphingolipids with alpha-hydroxylated fatty acid moieties, and an accumulation of sphingolipids without these moieties. In addition, the free trihydroxylated long-chain bases and ceramides were increased by five- and ten-fold, respectively, whereas the amount of glucosylceramides was decreased by 25%. Metabolite analysis of the double mutant revealed salicylates as enriched metabolites. Infection experiments supported the metabolic changes, as the double mutant showed an enhanced disease-resistant phenotype for infection with the obligate biotrophic pathogen Golovinomyces cichoracearum. In summary, these results suggest that fatty acid hydroxylation of ceramides is important for the biosynthesis of complex sphingolipids. Its absence leads to the accumulation of long-chain bases and ceramides as their precursors. This increases salicylate levels and resistance towards obligate biotrophic fungal pathogens, confirming a role of sphingolipids in salicylic acid-dependent defence reactions."],["dc.description.sponsorship","DFG Research Unit FOR546 [Fe 446/6, Ka 1209/3, FL3 INST 186/822-1]"],["dc.identifier.doi","10.1111/j.1469-8137.2012.04351.x"],["dc.identifier.isi","000310676400016"],["dc.identifier.pmid","23025549"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24836"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0028-646X"],["dc.title","Arabidopsis mutants of sphingolipid fatty acid alpha-hydroxylases accumulate ceramides and salicylates"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","406"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Developmental Cell"],["dc.bibliographiccitation.lastpage","420"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Sarikaya-Bayram, Oezlem"],["dc.contributor.author","Bayram, Oezguer"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Kim, Jong-Hwa"],["dc.contributor.author","Kim, Hee-Seo"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Chae, Keon-Sang"],["dc.contributor.author","Han, Dong-Min"],["dc.contributor.author","Han, Kap-Hoon"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T09:39:57Z"],["dc.date.available","2018-11-07T09:39:57Z"],["dc.date.issued","2014"],["dc.description.abstract","Epigenetic and transcriptional control of gene expression must be coordinated in response to external signals to promote alternative multicellular developmental programs. The membrane-associated trimeric complex VapA-VipC-VapB controls a signal transduction pathway for fungal differentiation. The VipC-VapB methyltransferases are tethered to the membrane by the FYVE-like zinc finger protein VapA, allowing the nuclear VelB-VeA-LaeA complex to activate transcription for sexual development. Once the release from VapA is triggered, VipC-VapB is transported into the nucleus. VipC-VapB physically interacts with VeA and reduces its nuclear import and protein stability, thereby reducing the nuclear VelB-VeA-LaeA complex. Nuclear VapB methyltransferase diminishes the establishment of facultative heterochromatin by decreasing histone 3 lysine 9 trimethylation (H3K9me3). This favors activation of the regulatory genes brlA and abaA, which promote the asexual program. The VapA-VipC-VapB methyltransferase pathway combines control of nuclear import and stability of transcription factors with histone modification to foster appropriate differentiation responses."],["dc.identifier.doi","10.1016/j.devcel.2014.03.020"],["dc.identifier.isi","000336608600005"],["dc.identifier.pmid","24871947"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33407"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","1878-1551"],["dc.relation.issn","1534-5807"],["dc.title","Membrane-Bound Methyltransferase Complex VapA-VipC-VapB Guides Epigenetic Control of Fungal Development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","964"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Molecular Microbiology"],["dc.bibliographiccitation.lastpage","979"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Nahlik, Krystyna"],["dc.contributor.author","Dumkow, Marc"],["dc.contributor.author","Bayram, Ozgür"],["dc.contributor.author","Helmstaedt, Kerstin"],["dc.contributor.author","Busch, Silke"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Gerke, Jennifer"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Schwier, Elke U."],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Westermann, Mieke"],["dc.contributor.author","Grond, Stephanie"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Goebel, Cornelia"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Meinicke, Peter"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-09-28T09:12:22Z"],["dc.date.available","2018-09-28T09:12:22Z"],["dc.date.issued","2010"],["dc.description.abstract","The COP9 signalosome complex (CSN) is a crucial regulator of ubiquitin ligases. Defects in CSN result in embryonic impairment and death in higher eukaryotes, whereas the filamentous fungus Aspergillus nidulans survives without CSN, but is unable to complete sexual development. We investigated overall impact of CSN activity on A. nidulans cells by combined transcriptome, proteome and metabolome analysis. Absence of csn5/csnE affects transcription of at least 15% of genes during development, including numerous oxidoreductases. csnE deletion leads to changes in the fungal proteome indicating impaired redox regulation and hypersensitivity to oxidative stress. CSN promotes the formation of asexual spores by regulating developmental hormones produced by PpoA and PpoC dioxygenases. We identify more than 100 metabolites, including orsellinic acid derivatives, accumulating preferentially in the csnE mutant. We also show that CSN is required to activate glucanases and other cell wall recycling enzymes during development. These findings suggest a dual role for CSN during development: it is required early for protection against oxidative stress and hormone regulation and is later essential for control of the secondary metabolism and cell wall rearrangement."],["dc.identifier.doi","10.1111/j.1365-2958.2010.07384.x"],["dc.identifier.pmid","21062371"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15841"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.relation.eissn","1365-2958"],["dc.title","The COP9 signalosome mediates transcriptional and metabolic response to hormones, oxidative stress protection and cell wall rearrangement during fungal development"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]