Balzarotti, Francisco

[["dc.bibliographiccitation.firstpage","606"],["dc.bibliographiccitation.issue","6325"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","612"],["dc.bibliographiccitation.volume","355"],["dc.contributor.author","Balzarotti, Francisco"],["dc.contributor.author","Eilers, Yvan"],["dc.contributor.author","Gwosch, Klaus C."],["dc.contributor.author","Gynnå, Arvid H."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Stefani, Fernando D."],["dc.contributor.author","Elf, Johan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:33:22Z"],["dc.date.available","2018-01-17T13:33:22Z"],["dc.date.issued","2017"],["dc.description.abstract","We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. In our experiments, 22 times fewer fluorescence photons are required as compared to popular centroid localization. In superresolution microscopy, MINFLUX attained ~1-nm precision, resolving molecules only 6 nanometers apart. MINFLUX tracking of single fluorescent proteins increased the temporal resolution and the number of localizations per trace by a factor of 100, as demonstrated with diffusing 30S ribosomal subunits in living Escherichia coli As conceptual limits have not been reached, we expect this localization modality to break new ground for observing the dynamics, distribution, and structure of macromolecules in living cells and beyond."],["dc.identifier.arxiv","1611.03401v1"],["dc.identifier.doi","10.1126/science.aak9913"],["dc.identifier.pmid","28008086"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11719"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1095-9203"],["dc.subject","Physics - Optics"],["dc.subject","Physics - Optics"],["dc.subject","Physics - Biological Physics"],["dc.title","Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6117"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","6122"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Eilers, Yvan"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Gwosch, Klaus C."],["dc.contributor.author","Balzarotti, Francisco"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:24Z"],["dc.date.available","2022-03-01T11:46:24Z"],["dc.date.issued","2018"],["dc.description.abstract","Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by centroid calculation of emission diffraction patterns. Since theoretical limits have not been reached, our results show that emerging MINFLUX nanoscopy bears great potential for dissecting the motions of individual (macro)molecules at hitherto-unattained combinations of spatial and temporal resolution."],["dc.description.abstract","Compared with localization schemes solely based on evaluating patterns of molecular emission, the recently introduced single-molecule localization concept called MINFLUX and the fluorescence nanoscopies derived from it require up to orders of magnitude fewer emissions to attain single-digit nanometer resolution. Here, we demonstrate that the lower number of required fluorescence photons enables MINFLUX to detect molecular movements of a few nanometers at a temporal sampling of well below 1 millisecond. Using fluorophores attached to thermally fluctuating DNA strands as model systems, we demonstrate that measurement times as short as 400 microseconds suffice to localize fluorescent molecules with ∼2-nm precision. Such performance is out of reach for popular camera-based localization by centroid calculation of emission diffraction patterns. Since theoretical limits have not been reached, our results show that emerging MINFLUX nanoscopy bears great potential for dissecting the motions of individual (macro)molecules at hitherto-unattained combinations of spatial and temporal resolution."],["dc.identifier.doi","10.1073/pnas.1801672115"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103658"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.title","MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","217"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","224"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Gwosch, Klaus C."],["dc.contributor.author","Pape, Jasmin K."],["dc.contributor.author","Balzarotti, Francisco"],["dc.contributor.author","Hoess, Philipp"],["dc.contributor.author","Ellenberg, Jan"],["dc.contributor.author","Ries, Jonas"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2022-03-01T11:46:02Z"],["dc.date.available","2022-03-01T11:46:02Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41592-019-0688-0"],["dc.identifier.pii","688"],["dc.identifier.pmid","31932776"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103535"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/43"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A07: Der Aufbau des synaptischen Cytoskeletts"],["dc.relation.eissn","1548-7105"],["dc.relation.issn","1548-7091"],["dc.relation.workinggroup","RG Hell"],["dc.rights.uri","http://www.springer.com/tdm"],["dc.title","MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]