Thiele, Jan Christoph

[["dc.bibliographiccitation.firstpage","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Sargsyan, Yelena"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Mougios, Nikolaos"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Thoms, Sven"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.date.accessioned","2022-01-13T06:27:32Z"],["dc.date.available","2022-01-13T06:27:32Z"],["dc.date.issued","2022-01-11"],["dc.description.abstract","DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s42003-021-02976-4"],["dc.identifier.pmid","35017652"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98096"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/392"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/415"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A10: Peroxisomen als modulatorische Einheiten im Herzstoffwechsel und bei Herzinsuffizienz"],["dc.relation.issn","2399-3642"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.relation.workinggroup","RG Thoms (Biochemistry and Molecular Medicine)"],["dc.rights","CC BY 4.0"],["dc.title","Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","740281"],["dc.bibliographiccitation.journal","Frontiers in Bioinformatics"],["dc.bibliographiccitation.volume","1"],["dc.contributor.affiliation","Thiele, Jan Christoph; \r\n\r\n1\r\nThird Institute of Physics—Biophysics, Georg August University, Göttingen, Germany"],["dc.contributor.affiliation","Nevskyi, Oleksii; \r\n\r\n1\r\nThird Institute of Physics—Biophysics, Georg August University, Göttingen, Germany"],["dc.contributor.affiliation","Helmerich, Dominic A.; \r\n\r\n2\r\nDepartment of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Würzburg, Germany"],["dc.contributor.affiliation","Sauer, Markus; \r\n\r\n2\r\nDepartment of Biotechnology and Biophysics, Biocenter, Julius-Maximilians-Universität Würzburg, Würzburg, Germany"],["dc.contributor.affiliation","Enderlein, Jörg; \r\n\r\n1\r\nThird Institute of Physics—Biophysics, Georg August University, Göttingen, Germany"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:47Z"],["dc.date.available","2022-01-12T16:50:47Z"],["dc.date.issued","2021"],["dc.date.updated","2022-09-04T02:08:32Z"],["dc.description.abstract","Fluorescence-lifetime single molecule localization microscopy (FL-SMLM) adds the lifetime dimension to the spatial super-resolution provided by SMLM. Independent of intensity and spectrum, this lifetime information can be used, for example, to quantify the energy transfer efficiency in Förster Resonance Energy Transfer (FRET) imaging, to probe the local environment with dyes that change their lifetime in an environment-sensitive manner, or to achieve image multiplexing by using dyes with different lifetimes. We present a thorough theoretical analysis of fluorescence-lifetime determination in the context of FL-SMLM and compare different lifetime-fitting approaches. In particular, we investigate the impact of background and noise, and give clear guidelines for procedures that are optimized for FL-SMLM. We do also present and discuss our public-domain software package “Fluorescence-Lifetime TrackNTrace,” which converts recorded fluorescence microscopy movies into super-resolved FL-SMLM images."],["dc.identifier.doi","10.3389/fbinf.2021.740281"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98095"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/406"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2673-7647"],["dc.relation.issn","2673-7647"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]