Thiele, Jan Christoph

[["dc.bibliographiccitation.firstpage","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Sargsyan, Yelena"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Mougios, Nikolaos"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Thoms, Sven"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.date.accessioned","2022-01-13T06:27:32Z"],["dc.date.available","2022-01-13T06:27:32Z"],["dc.date.issued","2022-01-11"],["dc.description.abstract","DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s42003-021-02976-4"],["dc.identifier.pmid","35017652"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98096"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/392"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/415"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A10: Peroxisomen als modulatorische Einheiten im Herzstoffwechsel und bei Herzinsuffizienz"],["dc.relation.issn","2399-3642"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.relation.workinggroup","RG Thoms (Biochemistry and Molecular Medicine)"],["dc.rights","CC BY 4.0"],["dc.title","Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]