Thiele, Jan Christoph

[["dc.bibliographiccitation.firstpage","38"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Communications Biology"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Sargsyan, Yelena"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Mougios, Nikolaos"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Gregor, Ingo"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Thoms, Sven"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.date.accessioned","2022-01-13T06:27:32Z"],["dc.date.available","2022-01-13T06:27:32Z"],["dc.date.issued","2022-01-11"],["dc.description.abstract","DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.1038/s42003-021-02976-4"],["dc.identifier.pmid","35017652"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98096"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/392"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/415"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A10: Peroxisomen als modulatorische Einheiten im Herzstoffwechsel und bei Herzinsuffizienz"],["dc.relation.issn","2399-3642"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.relation.workinggroup","RG Thoms (Biochemistry and Molecular Medicine)"],["dc.rights","CC BY 4.0"],["dc.title","Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","14190"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","ACS Nano"],["dc.bibliographiccitation.lastpage","14200"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic A."],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2021-03-05T08:58:21Z"],["dc.date.available","2021-03-05T08:58:21Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1021/acsnano.0c07322"],["dc.identifier.pmid","33035050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/80100"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/79"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-393"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1936-086X"],["dc.relation.issn","1936-0851"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.relation.workinggroup","RG Enderlein"],["dc.title","Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Thiele, Jan Christoph"],["dc.contributor.author","Helmerich, Dominic"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Butkevich, Eugenia"],["dc.contributor.author","Sauer, Markus"],["dc.contributor.author","Nevskyi, Oleksii"],["dc.contributor.author","Enderlein, Jörg"],["dc.date.accessioned","2022-01-12T16:50:38Z"],["dc.date.available","2022-01-12T16:50:38Z"],["dc.date.issued","2020"],["dc.description.abstract","Fluorescence lifetime imaging microscopy (FLIM) is an important technique that adds another dimension to the intensity and colour information of conventional microscopy. In particular, it allows for multiplexing fluorescent labels that have otherwise similar spectral properties. Currently, the only super-resolution technique that is capable of recording super-resolved images with lifetime information is STimulated Emission Depletion (STED) microscopy. In contrast, all Single-Molecule Localisation Microscopy (SMLM) techniques that employ wide-field cameras completely lack the lifetime dimension. Here, we combine Fluorescence-Lifetime Confocal Laser-Scanning Microscopy (FL-CLSM) with SMLM for realising single-molecule localisation-based fluorescence-lifetime super-resolution imaging (FL-SMLM). Besides yielding images with a spatial resolution much beyond the diffraction limit, it determines the fluorescence lifetime of all localised molecules. We validate our technique by applying it to direct STochastic Optical Reconstruction Microscopy (dSTORM) and Points Accumulation for Imaging in Nanoscale Topography (PAINT) imaging of fixed cells, and we demonstrate its multiplexing capability on samples with two different labels that differ only by fluorescence lifetime but not by their spectral properties."],["dc.format.extent","30"],["dc.identifier.doi","10.1101/2020.08.25.266387"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98093"],["dc.language.iso","en"],["dc.relation.orgunit","III. Physikalisches Institut - Biophysik"],["dc.title","Confocal Laser-Scanning Fluorescence-Lifetime Single-Molecule Localisation Microscopy"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]