Ramadori, Giuliano

[["dc.bibliographiccitation.firstpage","431"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","443"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:57:01Z"],["dc.date.available","2018-11-07T10:57:01Z"],["dc.date.issued","2007"],["dc.description.abstract","Synthesis of most of the plasma proteins is one of the main functions of the hepatocytes. Albumin synthesis is quantitatively the most abundant. In the present study we investigated albumin- and alpha-fetoprotein-gene-expression, and the function of the secretory apparatus during rat liver development. To this purpose we used the method of radioactive biosynthetic labeling of newly synthesized albumin and alpha-fetoprotein (AFP) to monitor the secretory capacity of endodermal cells derived from ventral foregut region (embryonic day 10, E10), and of embryonic and fetal hepatoblasts. Synthesis and secretion of albumin and AFP were already detected in the low numbered ventral foregut endodermal cells; fibrinogen synthesis was detectable in the E12 hepatoblasts, which were in higher number. The whole secretory machinery was functional from the earliest stages of liver development, and the speed of secretion was comparable with that of the adult hepatocytes. There was almost 4-fold increase of hepatoblasts cell volume in fetal stage compared with embryonic stage. The model used suggests that the hepatocyte secretory apparatus is already functional before the emergence of the liver bud. This is the first comparative report to analyze the hepatocyte secretory function, cell proliferation and cell volume during liver development."],["dc.identifier.doi","10.1007/s00418-007-0338-y"],["dc.identifier.isi","000249807600006"],["dc.identifier.pmid","17879097"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50146"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-119X"],["dc.relation.issn","0948-6143"],["dc.title","Kinetics of albumin- and alpha-fetoprotein-production during rat liver development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Hepatology"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Novosiadlyy, R."],["dc.contributor.author","Aprigliano, Isabella"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Bordoni, V."],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Tripodi, Marco"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:36:26Z"],["dc.date.available","2018-11-07T10:36:26Z"],["dc.date.issued","2006"],["dc.format.extent","S73"],["dc.identifier.doi","10.1016/S0168-8278(06)80175-3"],["dc.identifier.isi","000237328100175"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45324"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.publisher.place","Amsterdam"],["dc.relation.conference","41st Annual Meeting of the European-Association-for-the-Study-of-the-Liver"],["dc.relation.eventlocation","Vienna, AUSTRIA"],["dc.relation.issn","0168-8278"],["dc.title","Opposite effects of immortalized met murine hepatocytes on cell cycle and cell fate of rat liver hepatic stellate cells and liver myofibroblasts"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","BMC Comparative hepatology"],["dc.bibliographiccitation.lastpage","12"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Meier, Volker"],["dc.contributor.author","Tron, Kyrylo"],["dc.contributor.author","Batusic, Danko"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2019-07-09T11:41:48Z"],["dc.date.available","2019-07-09T11:41:48Z"],["dc.date.issued","2006"],["dc.description.abstract","Background: Alpha-fetoprotein (AFP) expression can resume in the adult liver under pathophysiological conditions. Orphan nuclear receptors were supposed to regulate AFP gene expression, in vitro. We were interested to study the expression of AFP and orphan nuclear receptors, in vivo. Results: The expression of AFP gene and orphan nuclear receptors in the liver was examined in different rat models: (a) fetal liver (b) liver regeneration [partial hepatectomy (PH) with and without 2-acetyl-aminofluren treatment (2-AAF)], (c) acute liver damage [treatment with CCl4] and (d) acute phase reaction treatment with turpentine oil]. After PH of 2-AAF treated rats, clusters of AFP positive cells occurred in the periportal region. In the Northern blot analysis, a positive hybridization signal for the full-length AFP-RNA was observed only in liver samples from 2-AAF treated rats after PH. In real-time PCR analysis, the full-length AFP-RNA was highly up regulated in the fetal liver (maximum at day 14: 21,500 fold); after PH of 2-AAF treated rats, the full-length AFPRNA was also up regulated up to 400 fold (day 7 after PH). The orphan nuclear receptors were down regulated at nearly each time points in all models, also at time point of up regulation of the AFP gene..."],["dc.identifier.doi","10.1186/1476-5926-5-2"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/1241"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58515"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","616"],["dc.subject.ddc","610"],["dc.title","Expression of AFP and Rev-Erb A/Rev-Erb B and N-CoR in fetal rat liver, liver injury and liver regeneration"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","11"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","19"],["dc.bibliographiccitation.volume","132"],["dc.contributor.author","Mansuroglu, Tuemen"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Joza, Tobias Z."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T08:28:11Z"],["dc.date.available","2018-11-07T08:28:11Z"],["dc.date.issued","2009"],["dc.description.abstract","The aim of this study was to determine whether passaged rat fetal liver cells are functional hepatoblasts. Hepatocyte/hepatoblast- and liver myofibroblast-gene-expressions were studied in adult and fetal rat liver tissues as well as in primary and passaged cultures of isolated rat fetal liver cells at both the mRNA and protein level. Desmin- and Alpha-Smooth Muscle Actin (SMA)-positive cells were located in the walls of liver vessels, whereas Desmin-positive/SMA-negative cells were distributed within the liver parenchyma. Primary cultures contained Prox1-positive hepatoblasts, Desmin/SMA-positive myofibroblasts and only a few Desmin-positive/SMA-negative cells. Albumin and alpha-fetoprotein (AFP) could be detected in the primary cultures and to a lesser extent after the first passage. The number of Desmin-positive/SMA-negative cells decreased with successive passage, such that after the second passage, only Desmin/SMA-positive cells could be detected. SMA-gene-expression increased during the passages, suggesting that myofibroblasts become the major cell population of fetal liver cell cultures over time. This observation needs to be taken into account, should passaged fetal liver cells be used for liver cell transplantation. Moreover it contradicts the concept of epithelial-mesenchymal transformation and suggests rather that selective overgrowth of mesenchymal cells occurs in culture."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SFB 402, C6, D3, D4]"],["dc.identifier.doi","10.1007/s00418-009-0596-y"],["dc.identifier.isi","000266822400002"],["dc.identifier.pmid","19381675"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3525"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16363"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0948-6143"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Hepatoblast and mesenchymal cell-specific gene-expression in fetal rat liver and in cultured fetal rat liver cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S134"],["dc.bibliographiccitation.journal","Journal of Hepatology"],["dc.bibliographiccitation.lastpage","S135"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Mansuroglu, Tuemen"],["dc.contributor.author","Batusic, D."],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Tron, Kyrylo"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Wilting, J."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:36:24Z"],["dc.date.available","2018-11-07T10:36:24Z"],["dc.date.issued","2006"],["dc.identifier.doi","10.1016/S0168-8278(06)80352-1"],["dc.identifier.isi","000237328100352"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45313"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.publisher.place","Amsterdam"],["dc.relation.conference","41st Annual Meeting of the European-Association-for-the-Study-of-the-Liver"],["dc.relation.eventlocation","Vienna, AUSTRIA"],["dc.relation.issn","0168-8278"],["dc.title","Analysis of the homeobox transcription factor Prox-1 expression in rat liver development and in different models of liver damage and regeneration"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","359"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","ANATOMY AND EMBRYOLOGY"],["dc.bibliographiccitation.lastpage","366"],["dc.bibliographiccitation.volume","208"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Hecht, Marie-Lyn"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Christ, B."],["dc.contributor.author","Tomarev, S. I."],["dc.contributor.author","von Kaisenberg, C. S."],["dc.contributor.author","Schweigerer, Lothar L."],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Wilting, J."],["dc.date.accessioned","2018-11-07T10:46:31Z"],["dc.date.available","2018-11-07T10:46:31Z"],["dc.date.issued","2004"],["dc.description.abstract","Prox1 is a transcription factor with two highly conserved domains, a homeobox and a prospero domain. It has been shown that Prox1 knock-out mice die during early embryonic stages and display a rudimentary liver. We have studied the expression of Prox1 at RNA and protein levels in chick, rat, mouse and human liver and in transformed and non-transformed hepatic cell lines. Prox1 is expressed in early embryonic hepatoblasts and is still expressed in adult hepatocytes. Prox1 protein is located in the nuclei of hepatoblasts, which grow into the neighboring embryonic mesenchyme. The expression pattern in chick, mouse, rat and human embryos is highly conserved. Besides albumin and alpha-fetal protein, Prox1 belongs to the earliest markers of the developing liver. In adult liver, Prox1 is expressed in hepatocytes but is absent from bile duct epithelial and non-parenchymal cells (Kupffer cells, hepatic stellate cells, sinusoidal endothelial cells and myofibroblasts). Isolated primary hepatocytes and hepatoma cell lines (HepG2, Hep3B) are Prox1 positive, whereas the immortalized murine liver cell-line MMH, which constitutively expresses the receptor c-met, is Prox1 negative. Transfection of MMH with Prox1 cDNA increases the expression level significantly as compared to control transfectants. In HepG2 and Hep3B, the Prox1 levels are even up to 100 times higher. Our studies show that Prox1 is a highly conserved transcription factor, expressed in hepatocytes from the earliest stages of development into adulthood and over-expressed in hepatoma cell lines. Its absence from bile duct epithelial cells suggests a function for the specification of hepatoblasts into hepatocytes. The genes controlled by Prox1 need to be studied in the future."],["dc.identifier.doi","10.1007/s00429-004-0403-4"],["dc.identifier.isi","000223266100003"],["dc.identifier.pmid","15232737"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47763"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0340-2061"],["dc.title","The homeobox transcription factor Prox1 is highly conserved in embryonic hepatoblasts and in adult and transformed hepatocytes, but is absent from bile duct epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","549"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Histochemistry and Cell Biology"],["dc.bibliographiccitation.lastpage","562"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Elmaouhoub, Abderrahim"],["dc.contributor.author","Mansuroglu, Tuemen"],["dc.contributor.author","Batusic, Danko"],["dc.contributor.author","Tron, Kyrylo"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Papoutsi, Maria"],["dc.contributor.author","Pieler, Tomas"],["dc.contributor.author","Wilting, Joerg"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T09:01:16Z"],["dc.date.available","2018-11-07T09:01:16Z"],["dc.date.issued","2006"],["dc.description.abstract","The aim of this study was to analyse the changes of Prospero-related homeobox 1 (Prox1) gene expression in rat liver under different experimental conditions of liver injury, regeneration and acute phase reaction, and to correlate it with that of markers for hepatoblasts, hepatocytes, cholangiocytes and oval cells. Gene expression was studied at RNA level by RT-PCR, and at protein level by immunohistochemistry. At embryonal stage of rat liver development (embryonal days (ED) 14-16) hepatoblasts were found to be Prox1(+)/Cytokeratin (CK) 19(+) and alpha-fetoprotein (AFP)(+), at this stage Prox1(-)/CK19(+)/AFP(-) small cells (early cholangiocytes?) were identified. In fetal liver (ED 18-22) hepatoblasts were Prox1(+)/CK19(-)/AFP(+). CK7(+) cholangiocytes were detected at this stage, and they were Prox1(-)/AFP(-). In the adult liver hepatocytes were Prox1(+)/CK19(-)/CK7(-)/AFP(-), cholangiocytes were CK19(+) and/or CK7(+) and AFP(-)/Prox1(-). In models of liver damage and regeneration Prox1 remained a stable marker of hepatocytes. After 2-acetyl-aminofluorene treatment with partial hepatectomy (AAF/PH) the amount of Prox1 specific transcripts was low in the liver, when CK19 and AFP gene expression was high, and at no time point AFP(+)/CK19(+) \"oval cells\" were found to be Prox1(+). However, a few Prox1(+)/CK19(+) and a few Prox1(+)/CK7(+) cells were identified in the liver of AAF/PH-animals, which may represent precursors of hepatocytes, or a precancerous state."],["dc.identifier.doi","10.1007/s00418-006-0191-4"],["dc.identifier.isi","000240980900003"],["dc.identifier.pmid","16770575"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24380"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0948-6143"],["dc.title","Prospero-related homeobox 1 (Prox1) is a stable hepatocyte marker during liver development, injury and regeneration, and is absent from \"oval cells\""],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]