Ramadori, Giuliano

[["dc.bibliographiccitation.journal","Strahlentherapie und Onkologie"],["dc.bibliographiccitation.volume","183"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Guerleyen, Hakan"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Hess, C. F."],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Saile, Bernhard"],["dc.date.accessioned","2018-11-07T11:02:14Z"],["dc.date.available","2018-11-07T11:02:14Z"],["dc.date.issued","2007"],["dc.format.extent","49"],["dc.identifier.isi","000247071800131"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51329"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Vogel"],["dc.publisher.place","Munich"],["dc.relation.conference","13th Congress of the Deutschen-Gesellschaft-fur-Radioonkologie"],["dc.relation.eventlocation","Hannover, GERMANY"],["dc.relation.issn","0179-7158"],["dc.title","Radiation-induced reduced expression of Hsp27 in in vitro liver macrophages leads to apoptosis and release of TNF-alpha"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","301"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cell and Tissue Research"],["dc.bibliographiccitation.lastpage","311"],["dc.bibliographiccitation.volume","313"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","El-Armouche, H."],["dc.contributor.author","Aprigliano, Isabella"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:36:23Z"],["dc.date.available","2018-11-07T10:36:23Z"],["dc.date.issued","2003"],["dc.description.abstract","Hepatic stellate cells (HSCs), the pericytes of hepatic sinusoids, and liver myofibroblasts (rMFs), cells located in the portal field and around the pericentral area, are the principal fibrogenic cell types of the liver. In cases of liver damage HSCs undergo 'activation,' i.e., they acquire a myofibroblast-like appearance and synthesize huge amounts of extracellular matrix proteins (ECMs). Their proliferation ability, however, is a matter of debate. In fact, during culture the number of rat HSCs decreases, while DNA synthesis activity and DNA content per cell increase (4+/-0.6 times). Together with the decrease in cell number (60+/-19% at day 6 of primary culture compared to day 3), cell volume increases and many HSCs become multinuclear. On the other hand, in cultures of rMFs, cell number increases along with DNA synthesis, and these cells do not become multinuclear. 'Activated' HSCs produce higher levels of cyclin D-1 and E-1 transcripts than rMFs, which correlates with their increased levels of phosphorylated retinoblastoma (Rb) protein. In activated HSCs DNA synthesis seems to be associated with polyploidy and increase in cell volume, while DNA synthesis is followed by mitosis in rMFs."],["dc.identifier.doi","10.1007/s00441-003-0768-3"],["dc.identifier.isi","000185457000006"],["dc.identifier.pmid","12898209"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45309"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0302-766X"],["dc.title","Endoreplication and polyploidy in primary culture of rat hepatic stellate cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S479"],["dc.bibliographiccitation.journal","Radiotherapy and Oncology"],["dc.bibliographiccitation.lastpage","S480"],["dc.bibliographiccitation.volume","81"],["dc.contributor.author","Rave-Fraenk, Margret"],["dc.contributor.author","Guerleyen, Hakan"],["dc.contributor.author","Christiansen, H."],["dc.contributor.author","Thello, K."],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Hermann, Robert Michael"],["dc.contributor.author","Hess, C. F."],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Saile, Bernhard"],["dc.date.accessioned","2018-11-07T09:13:58Z"],["dc.date.available","2018-11-07T09:13:58Z"],["dc.date.issued","2006"],["dc.identifier.isi","000242719101490"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27292"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Ireland Ltd"],["dc.publisher.place","Clare"],["dc.relation.conference","Conference of the Spanish-Portuguese-and -Latin-American-Association"],["dc.relation.eventlocation","Leipzig, GERMANY"],["dc.relation.issn","0167-8140"],["dc.title","Irradiation leads to sensitization of hepatocytes to TNF-alpha- mediated apoptosis by upregulation of IKB expression"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","189"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Radiology"],["dc.bibliographiccitation.lastpage","197"],["dc.bibliographiccitation.volume","242"],["dc.contributor.author","Christiansen, Hans"],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Reuter, Felix"],["dc.contributor.author","Rave-Frank, Margret"],["dc.contributor.author","Hermann, Robert Michael"],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Hille, Andrea"],["dc.contributor.author","Hess, Clemens Friedrich"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T11:07:32Z"],["dc.date.available","2018-11-07T11:07:32Z"],["dc.date.issued","2007"],["dc.description.abstract","Purpose: To prospectively analyze hepcidin, hemojuvelin and ferro-portin-1 expression after x-irradiation or rat liver and isolated rat hepacocytes. Materials and Methods: The treatment of the rats and this study were improved by the local committee and the public authority on animal welfare. Rat livers in vivo and isolated rat hepatocytes in vitro were irradiated. The total number of rats in this study was 43. RNA extracted from livers (1, 3, 6, 12, 24, and 48 hours after irradiation) and from hepatocytes (1, 3, 6, 12, and 24 hours after irradiation) was analyzed with real-time polymerase chain reaction and Northern blot. Cytokines and prohepcidin in serum of irradiated rats were quantitatively detected with enzyme-linked immunosorbent assay. Sham-irradiated animals served as controls in all experiments. Differences between sham-irradiated and irradiated data groups were tested with anaylsis of variance and Dunnett post hoc test. Results: In vivo, a significant radiation-induced increase of hepcidin (P = .034), interleukin (IL) 1 beta (P = .008), IL-6 (P < .011), and tumor necrosis factor alpha (TNF-alpha) (P = .047) expression could be detected within the first 48 hours after irradiation. Expression of hemojuvelin (P = .008) and ferro-portin-1 (P = .002) was significantly decreased. Serum iron levels were decreased because of irradiation (P < .058); prohepcidin serum levels were increased (P = .05). In rat hepatocytes in vitro, hepcidin RNA levels were significantly downregulated after irradiation (P < .001). Incubation of irradiated hepatocytes with IL-1 beta, IL-6, or TNNF-alpha led to upregulation of hepcidin expression in vitro up to 6 hours after irradiation, with subsequent significant down-regulation for incubation with IL-1 beta (P < .001). Hemojuvelin expression behaved in a way opposite to that of hepcidin. Conclusion: x-Irradiation of the liver, induced changes of hepcidin gene expression that are probably induced by acute phase mediators produced within the liver, itself."],["dc.identifier.doi","10.1148/radiol.2421060083"],["dc.identifier.isi","243842500024"],["dc.identifier.pmid","17090718"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52584"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Radiological Soc North America"],["dc.relation.issn","0033-8419"],["dc.title","x-irradiation in rat liver: Consequent upregulation of hepcidin and downregulation of hemojuvelin and ferroportin-1 gene expression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","469"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","476"],["dc.bibliographiccitation.volume","83"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Eisenbach, C."],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","El-Armouche, H."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:45:54Z"],["dc.date.available","2018-11-07T10:45:54Z"],["dc.date.issued","2004"],["dc.description.abstract","The activated hepatic stellate cell (HSC) is an important fibrogenic cell type of the liver. Interferon-alpha (IFN-alpha) has recently been shown to elicit an antiapoptotic effect on activated HSC by a JAK-2-dependent inhibition of caspase-8 activation. As JAK-2 has so far been shown to be a member of the IFN-gamma signal transduction pathway we studied the effect of IFN-gamma on apoptosis as well as on its signaling in primary cultured rat HSC. IFN-gamma elicited a proapoptotic effect in activated HSC. The combination of both, IFN-gamma and IFN-alpha, however, completely cancelled each other's effect. No effect of the two cytokines on major members of apoptosis-regulating systems (CD95, CD95L, bcl-2, bax, bcl-xL, p53, p21(WAFI), p27, NFkappaB) could be observed. Western Blot analysis revealed that gene expression of the chaperone HSP70 was found to be downregulated by IFN-gamma but upregulated by IFN-alpha. The effect could be abrogated by administration of both. After transfection of activated HSC with a pCMV-HSP70 M expression vector the proapoptotic effect of IFN-gamma was cancelled. Using HSP70 antisense, the antiapoptotic effect of IFN-alpha was cancelled as well. However IFN-gamma had no effect on upregulation of JAK-2 and pJAK-2 by IFN-alpha. Taken together IFN-gamma and IFN-alpha exert opposite effects on apoptosis in HSC. This effect is mediated by their counteracting effect on HSP70 expression which acts antiapoptotic at the level of caspase-8."],["dc.identifier.doi","10.1078/0171-9335-00409"],["dc.identifier.isi","000224916400003"],["dc.identifier.pmid","15540463"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47614"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Urban & Fischer Verlag"],["dc.relation.issn","0171-9335"],["dc.title","Interferon-gamma acts proapoptotic on hepatic stellate cells (HSC) and abrogates the antiapoptotic effect of interferon-alpha by an HSP70-dependant pathway"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","G435"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","AJP Gastrointestinal and Liver Physiology"],["dc.bibliographiccitation.lastpage","G444"],["dc.bibliographiccitation.volume","283"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Matthes, N."],["dc.contributor.author","Neubauer, K."],["dc.contributor.author","Eisenbach, C."],["dc.contributor.author","El-Armouche, H."],["dc.contributor.author","Dudas, Jozsef"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T10:11:28Z"],["dc.date.available","2018-11-07T10:11:28Z"],["dc.date.issued","2002"],["dc.description.abstract","Hepatic stellate cells (HSC), particularly activated HSC, are thought to be the principle matrix-producing cell of the diseased liver. However, other cell types of the fibroblast lineage, especially the rat liver myofibroblasts (rMF), also have fibrogenic potential. A major difference between the two cell types is the different life span under culture conditions. Although nearly no spontaneous apoptosis could be shown in rMF cultures, 18 +/- 2% of the activated HSC (day 7) were apoptotic. Compared with activated HSC, CD95R was expressed in 70% higher amounts in rMF. CD95L could only be detected in activated HSC. Stimulation of the CD95 system by agonistic antibodies (1 ng/ml) led to apoptosis of all rMF within 2 h, whereas activated HSC were more resistant (5.3 h/ 40% of total cells). Although transforming growth factor-beta downregulated apoptosis in both activated HSC and rMF, tumor necrosis factor-alpha (TNF-alpha) upregulated apoptosis in rMF. Lack of spontaneous apoptosis and CD95L expression in rMF and the different reaction on TNF-alpha stimulation reveal that activated HSC and rMF belong to different cell populations."],["dc.identifier.doi","10.1152/ajpgi.00441.2001"],["dc.identifier.isi","000176842200023"],["dc.identifier.pmid","12121892"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40050"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Physiological Soc"],["dc.relation.issn","0193-1857"],["dc.title","Rat liver myofibroblasts and hepatic stellate cells differ in CD95-mediated apoptosis and response to TNF-alpha"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Hepatology"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Matthes, N."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T11:09:24Z"],["dc.date.available","2018-11-07T11:09:24Z"],["dc.date.issued","2000"],["dc.format.extent","87"],["dc.identifier.doi","10.1016/S0168-8278(00)80665-0"],["dc.identifier.isi","000087104500237"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53002"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Munksgaard Int Publ Ltd"],["dc.publisher.place","Copenhagen"],["dc.relation.issn","0168-8278"],["dc.title","Effect of TGF-beta and TNF-alpha on apoptosis and proliferation of liver myofibroblasts (rMF) and activated hepatic stellate cells (HSC)"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Hepatology"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Scharf, Jens-Gerd"],["dc.contributor.author","Dombrowski, F."],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Eisenbach, C."],["dc.contributor.author","Demori, I."],["dc.contributor.author","Braulke, Thomas"],["dc.contributor.author","Hartmann, Hans"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T11:09:21Z"],["dc.date.available","2018-11-07T11:09:21Z"],["dc.date.issued","2000"],["dc.format.extent","76"],["dc.identifier.doi","10.1016/S0168-8278(00)80625-X"],["dc.identifier.isi","000087104500197"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52991"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Munksgaard Int Publ Ltd"],["dc.publisher.place","Copenhagen"],["dc.relation.issn","0168-8278"],["dc.title","IGFBP-1 is highly induced during acute CCl4 liver injury and potentiates the IGF-I-stimulated proliferation of rat hepatic stellate cells (HSC)"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Hepatology"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Neubauer, K."],["dc.contributor.author","Armbrust, T."],["dc.contributor.author","Kuethe, F."],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T09:59:46Z"],["dc.date.available","2018-11-07T09:59:46Z"],["dc.date.issued","2002"],["dc.format.extent","329A"],["dc.identifier.isi","000178301700648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37663"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","W B Saunders Co"],["dc.publisher.place","Philadelphia"],["dc.relation.conference","53rd Annual Meeting of the Association-for-the-Study-of-Liver-Diseases (AASLD)"],["dc.relation.eventlocation","BOSTON, MASSACHUSETTS"],["dc.relation.issn","0270-9139"],["dc.title","Gadoliniumchloride treatment of rat livers induces a functional alteration but not a depletion of Kupffer cells."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Hepatology"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Saile, Bernhard"],["dc.contributor.author","Eisenbach, C."],["dc.contributor.author","Neubauer, K."],["dc.contributor.author","Stoeckigt, C."],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T09:36:44Z"],["dc.date.available","2018-11-07T09:36:44Z"],["dc.date.issued","2000"],["dc.format.extent","191A"],["dc.identifier.isi","000089622400114"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32680"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","W B Saunders Co"],["dc.publisher.place","Philadelphia"],["dc.relation.issn","0270-9139"],["dc.title","Antiapoptotic effect of interferon-A versus proapoptotic effect of interferon-Gamma on hepatic stellate cells (RSC) - A novel pathway of IFN-A signal transduction via janus kinase 2 (JAK2) and caspase 8."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]