Becker, Heiko C.

[["dc.bibliographiccitation.firstpage","2195"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Crop Science"],["dc.bibliographiccitation.lastpage","2199"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","zum Felde, Thomas"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T09:18:08Z"],["dc.date.available","2018-11-07T09:18:08Z"],["dc.date.issued","2006"],["dc.description.abstract","Improving the meal and protein quality for feed and food purposes is of increasing importance in canola (Brassica napus L.). The phenolic acid ester content contributes to the bitter taste, astringency, and dark color of rapeseed meal products. The predominant phenolic acid esters are sinapate esters (SE), which make up 1 to 2% of the seed dry matter. The objective of the present study was to analyze the genetic variation and the genotype X environment interactions for SE content and composition in three populations of doubled haploid lines. The populations were grown in three to four environments in Germany. The following SE were analyzed by HPLC: sinapoylcholine (sinapine), sinapoylglucose, and a minor group of other SE which includes sinapate. The three populations showed a highly significant variation for the total SE content, and sinapine was the predominant sinapate ester compound. The analysis of variance showed highly significant effects for the genotype (G), the environment (E) and the G X E interactions for all three populations. In two of the populations the G X E interaction variance components were less than half of the genetic variance, in one population it was slightly higher. The estimates for heritability of the individual and total SE were generally high and ranged from 0.57 to 0.93. A reduction of sinapate ester content was not associated with a change in oil, protein, and glucosinolate content."],["dc.identifier.doi","10.2135/cropsci2006.03.0155"],["dc.identifier.isi","000240821800045"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28335"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Crop Science Soc Amer"],["dc.relation.issn","0011-183X"],["dc.title","Genotype x environment interactions, heritability, and trait correlations of sinapate ester content in winter rapeseed (Brassica napus L.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","115"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Breeding"],["dc.bibliographiccitation.lastpage","125"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Zhao, Jianyi"],["dc.contributor.author","Dimov, Zoran"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:20:23Z"],["dc.date.available","2018-11-07T11:20:23Z"],["dc.date.issued","2008"],["dc.description.abstract","Increasing oil content and improving the fatty acid composition in the seed oil are important breeding goals for rapeseed (Brassica napus L.). The objective of the study was to investigate a possible relationship between fatty acid composition and oil content in an oilseed rape doubled haploid (DH) population. The DH population was derived from a cross between the German cultivar Sollux and the Chinese cultivar Gaoyou, both having a high erucic acid and a very high oil content. In total, 282 DH lines were evaluated in replicated field experiments in four environments, two each in Germany and in China. Fatty acid composition of the seed oil was analyzed by gas liquid chromatography and oil content was determined by NIRS. Quantitative trait loci (QTL) for fatty acid contents were mapped and their additive main effects were determined by a mixed model approach using the program QTLMapper. For all fatty acids large and highly significant genetic variations among the genotypes were observed. High heritabilities were determined for oil content and for all fatty acids (h(2) = 0.82 to 0.94), except for stearic acid content (h(2) = 0.38). Significant correlations were found between the contents of all individual fatty acids and oil content. Closest genetic correlations were found between oil content and the sum of polyunsaturated fatty acids (18:2 + 18:3; r(G) = -0.46), the sum of monounsaturated fatty acids (18:1 + 20:1 + 22:1; r(G) = 0.46) and palmitic acid (16:0; r(G) = -0.34), respectively. Between one and eight QTL for the contents of the different fatty acids were detected. Together, their additive main effects explained between 28% and 65% of the genetic variance for the individual fatty acids. Ten QTL for fatty acid contents mapped within a distance of 0 to 10 cM to QTL for oil content, which were previously identified in this DH population. QTL mapped within this distance to each other are likely to be identical. The results indicate a close interrelationship between fatty acid composition and oil content, which should be considered when breeding for increased oil content or improved oil composition in rapeseed."],["dc.identifier.doi","10.1007/s11032-007-9113-y"],["dc.identifier.isi","000251321400010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55523"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1572-9788"],["dc.relation.issn","1380-3743"],["dc.title","Mapping QTL controlling fatty acid composition in a doubled haploid rapeseed population segregating for oil content"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2405"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","2415"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Schatzki, Joerg"],["dc.contributor.author","Schoo, Burkhard"],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T09:20:52Z"],["dc.date.available","2018-11-07T09:20:52Z"],["dc.date.issued","2013"],["dc.description.abstract","Following winter oilseed rape cultivation, considerable numbers of volunteer oilseed rape plants may occur in subsequent years in following crops. The appearance of volunteer oilseed rape plants is based on the capability of the seeds to become secondary dormant and to survive in this stage for many years in the soil. Genetic reduction of secondary seed dormancy in oilseed rape could provide a means to reduce the frequency of volunteer plants and especially the dispersal of transgenic oilseed rape. The objective of the present study was to analyse the inheritance of primary and secondary seed dormancy in a winter oilseed rape doubled haploid population derived from the cross Express 617 x R53 and to study correlations to other seed traits. Field experiments were performed in Germany for 2 years at two locations with two replicates. Seeds harvested from open pollinated plants were used for all analyses, including a laboratory test for seed dormancy. A previously developed molecular marker map of the doubled haploid population was used to map QTL of the relevant traits. For primary, secondary and total seed dormancy, the results showed significant effects of the genotypes and their interactions, with years and locations. Two, four and five QTL were detected for primary, secondary and total seed dormancy which explained 19, 35 and 42 % of the phenotypic variance, respectively. Results show that secondary seed dormancy is a heritable trait and that selection for low secondary seed dormancy is possible."],["dc.description.sponsorship","German Federal Ministry of Education and Research (BMBF) [FKZ 0315211C]"],["dc.identifier.doi","10.1007/s00122-013-2144-3"],["dc.identifier.isi","000323671100017"],["dc.identifier.pmid","23783224"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28977"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0040-5752"],["dc.title","Mapping of QTL for seed dormancy in a winter oilseed rape doubled haploid population"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","27"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Euphytica"],["dc.bibliographiccitation.lastpage","34"],["dc.bibliographiccitation.volume","153"],["dc.contributor.author","Hom, Nang Hseng"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:06:31Z"],["dc.date.available","2018-11-07T11:06:31Z"],["dc.date.issued","2007"],["dc.description.abstract","Near infrared-reflectance spectroscopy (NIRS) is widely used as an efficient and non-destructive method for seed quality analysis in oilseed rape (Brassica napus L.). Normally, calibration equations are developed for seed samples sizes of 3-4 g. However, during the breeding process much less than 3 g of samples are frequently only available for analysis. The objective of the present study was to analyse whether calibration equations developed for standard sample sizes can be used to predict the oil, protein, moisture, glucosinolate and fatty acid content of sample sizes ranging from about 450 to 5 mg (single seeds). Special adapters were made for the standard ring cups to hold the smaller samples during NIRS scanning. Close Spearman rank correlations (r(S) = 0.82-1.00 ) showed that NIRS calibration equations developed for standard small ring cups can be used to predict the oil, protein, and glucosinolate content of smaller samples of 450-100 mg seeds. For oleic, linolenic and erucic acid, close correlations were only found for adapter sizes holding 450-250 mg seeds (r (S) = 0.79-0.98 ). Only moderate to very low correlations were found for the moisture content. The standard NIRS calibration equations could also be used to predict the oil content (R-2 = 0.79 ) and the protein content (R-2 = 0.72 ) of single seeds (ca. 5 mg), whereas this was not possible for the glucosinolate content (R-2 = 0.54 ). Therefore, single seed NIRS calibration equations were developed for alkenyl, indole and total glucosinolate content. The coefficients of determinations in cross validation ranged from 0.83 (alkenyl) to 0.86 (indole and total glucosinolates)."],["dc.identifier.doi","10.1007/s10681-006-9195-3"],["dc.identifier.isi","000242852500004"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52332"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0014-2336"],["dc.title","Non-destructive analysis of rapeseed quality by NIRS of small seed samples and single seeds"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","493"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Crop Science"],["dc.bibliographiccitation.lastpage","500"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Hom, Nang Hseng"],["dc.contributor.author","Schierholt, Antje"],["dc.contributor.author","Moellers, Christian"],["dc.contributor.author","Becker, Heiko C."],["dc.date.accessioned","2018-11-07T10:00:05Z"],["dc.date.available","2018-11-07T10:00:05Z"],["dc.date.issued","2015"],["dc.description.abstract","The genetics of seed compounds is complex because seed quality traits might be exclusively determined by the genotype of the female parent or, depending on the genotype of the embryo, may also be affected by the genotype of the pollen parent. For many quality traits of oilseed rape (Brassica napus L.), the influence of the pollen parent is not well known. In this study, two male sterile lines (female parent) were pollinated by 10 genotypes (pollen parent) differing largely in seed quality. Field experiments were conducted at three locations in Northern Germany, with two replications in 1 yr. Pollen parents were sown in plots and 10 male sterile plants from each female parent were hand planted into the center of each plot. Seeds were harvested from pollen parents and from the interplanted female parents. Seeds were analyzed for oil, protein, and sinapic acid esters by near-infrared reflectance spectroscopy; for glucosinolate and tocopherol content by high-performance liquid chromatography; and for fatty acid content by gas liquid chromatography. Earlier results were confirmed in that fatty acid composition is dependent on the embryo genotype and is therefore largely influenced by the pollen parent. Significant pollen parent effects were also observed for oil content, indole glucosinolate, sinapoylglucose, total sinapic acid ester, and gamma-tocopherol contents. The F-1 seed quality was positively correlated with the pollen parent seed quality. In conclusion, not only the fatty acid composition but also other seed quality traits such as oil content are influenced by the genotype of the pollen parent. This should be considered when analyzing open pollinated plants."],["dc.identifier.doi","10.2135/cropsci2014.04.0284"],["dc.identifier.isi","000352223000003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37723"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Crop Science Soc Amer"],["dc.relation.issn","1435-0653"],["dc.relation.issn","0011-183X"],["dc.title","Pollen Genotype Effects on Seed Quality Traits in Winter Oilseed Rape"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1000"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Crop Science"],["dc.bibliographiccitation.lastpage","1006"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Amar, Samija"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:15:26Z"],["dc.date.available","2018-11-07T11:15:26Z"],["dc.date.issued","2008"],["dc.description.abstract","Phytosterols are natural constituents of vegetable oils and are known for their cholesterol-lowering properties. The oil of rapeseed (Brassica napus L.) is one of the richest natural sources of phytosterols. Genetically enhancing the phytosterol content could give an added value to the rapeseed oil and derived products. Our objectives were to develop a gas-liquid chromatographic method for the analysis of phytosterol content in seeds of oilseed rape, to determine the genetic variation and the genotype x environment interactions, and to estimate correlations between phytosterols and other important seed quality traits in three doubled haploid populations of winter rapeseed. The populations were tested during several years in three to four environments. Sitosterol and campesterol were detected as the two major phytosterols followed by brassicasterol, avenasterol, and stigmasterol. Large differences were found in total phytosterol content (2.57 to 4.15 g kg(-1) seed), with predominant genetic variance components resulting in high heritabilities ranging from 0.84 to 0.91. Phytosterol content was not negatively correlated with oil content and there were no close correlations to protein and glucosinolate content. The large genetic variation along with high heritabilities indicate that an effective breeding for enhanced phytosterol content and modified composition should be possible without negative impacts on oil, protein, or glucosinolate content."],["dc.identifier.doi","10.2135/cropsci2007.10.0578"],["dc.identifier.isi","000256635400017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54364"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Crop Science Soc Amer"],["dc.relation.issn","0011-183X"],["dc.title","Genetic variation and genotype x environment interactions of phytosterol content in three doubled haploid populations of winter rapeseed"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","78"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Plant Breeding"],["dc.bibliographiccitation.lastpage","83"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Amar, Samija"],["dc.contributor.author","Becker, H. C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:33:18Z"],["dc.date.available","2018-11-07T08:33:18Z"],["dc.date.issued","2009"],["dc.description.abstract","Rapeseed oil is one of the richest natural sources of phytosterols, known to reduce the LDL-cholesterol levels, one of the major cardiovascular disease risk factors. Increasing the phytosterol content in rapeseed could give an added value to the oil and derived products. Our objective was to analyse the genetic variation of phytosterol content in modern winter rapeseed cultivars and resynthesized lines following field experiments and to develop a near-infrared reflectance spectroscopic (NIRS) calibration for high throughput estimation of phytosterol content. Phytosterol content as analysed by gas-liquid chromatography ranged from 3565 to 4800 mg/kg seed for modern cultivars and from 2079 to 4329 mg/kg seed for resynthesized lines. The NIRS calibration showed a high fraction of explained variance in cross-validation of 0.81 for total phytosterol content and the standard error of cross-validation was 241 mg/kg. The results show that the cultivars contain already high phytosterol contents. The NIRS calibrations developed for total phytosterol content should be useful for germplasm screening and in breeding programmes aimed at increasing the phytosterol content in rapeseed."],["dc.description.sponsorship","Hans-Bockler-Stiftung"],["dc.identifier.doi","10.1111/j.1439-0523.2008.01531.x"],["dc.identifier.isi","000262830600010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17543"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0179-9541"],["dc.title","Genetic variation in phytosterol content of winter rapeseed (Brassica napus L.) and development of NIRS calibration equations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1213"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","1222"],["dc.bibliographiccitation.volume","127"],["dc.contributor.author","Schatzki, Joerg"],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T09:40:38Z"],["dc.date.available","2018-11-07T09:40:38Z"],["dc.date.issued","2014"],["dc.description.abstract","Key message Cruciferin (cru) and napin (nap) were negatively correlated and the cru/nap ratio was closely negative correlated with glucosinolate content indicating a link between the two biosynthetic pathways. Abstract Canola-type oilseed rape (Brassica napus L.) is an economically important oilseed crop in temperate zones. Apart from the oil, the canola protein shows potential as a value-added food and nutraceutical ingredient. The two major storage protein groups occurring in oilseed rape are the 2 S napins and 12 S cruciferins. The aim of the present study was to analyse the genetic variation and the inheritance of napin and cruciferin content of the seed protein in the winter oilseed rape doubled haploid population Express 617 x R 53 and to determine correlations to other seed traits. Seed samples were obtained from field experiments performed in 2 years at two locations with two replicates in Germany. A previously developed molecular marker map of the DH population was used to map quantitative trait loci (QTL) of the relevant traits. The results indicated highly significant effects of the year and the genotype on napin and cruciferin content as well as on the ratio of cruciferin to napin. Heritabilities were comparatively high with 0.79 for napin and 0.77 for cruciferin. Napin and cruciferin showed a significant negative correlation (-0.36 ) and a close negative correlation of the cru/nap ratio to glucosinolate content was observed (-0.81 ). Three QTL for napin and two QTL for cruciferin were detected, together explaining 47 and 35 % of the phenotypic variance. A major QTL for glucosinolate content was detected on linkage group N19 whose confidence interval overlapped with QTL for napin and cruciferin content. Results indicate a relationship between seed protein composition and glucosinolate content."],["dc.description.sponsorship","German Federal Ministry of Education and Research (BMBF) [FKZ 0315211C]"],["dc.identifier.doi","10.1007/s00122-014-2292-0"],["dc.identifier.isi","000336754700016"],["dc.identifier.pmid","24595811"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33548"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-2242"],["dc.relation.issn","0040-5752"],["dc.title","Mapping of QTL for the seed storage proteins cruciferin and napin in a winter oilseed rape doubled haploid population and their inheritance in relation to other seed traits"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","291"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Plant Breeding"],["dc.bibliographiccitation.lastpage","296"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Zum Felde, T."],["dc.contributor.author","Baumert, A."],["dc.contributor.author","Strack, D."],["dc.contributor.author","Becker, H. C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:02:09Z"],["dc.date.available","2018-11-07T11:02:09Z"],["dc.date.issued","2007"],["dc.description.abstract","Increasing the meal and protein quality of winter rapeseed ( Brassica napus L.) for food and feed purposes is gaining importance in rapeseed breeding programmes. Rapeseed meal has a high content of phenolic acid esters, mainly sinapate esters, which have been shown to cause a dark colour and a bitter taste in rapeseed meal and derived protein products. The aim of the present study was to analyse the genetic variation for individual and total sinapate ester content, to develop Near Infrared Reflectance Spectroscopic (NIRS) calibrations, and to identify genotypes with a low sinapate ester content after testing in the. field. The following sinapate esters were analysed by HPLC: sinapoyl-choline (sinapine), sinapoylglucose, and a minor group of 'other sinapate esters' which includes free sinapate. A genotypically diverse set of seed samples of winter oilseed rape (old and new cultivars, breeding lines, resynthesized rapeseed) from different years and locations was collected, their NIRS spectra recorded and the samples were further analysed by HPLC. The complete NIRS calibration seed sample set (n = 575) showed a large variation in total sinapate ester content, ranging from 3.2 to 12.7 mg sinapate equivalents per g seeds. The NIRS calibration equations showed high fractions of explained variances in cross validation (R-cv(2)) ranging from 0.75 (other sinapate esters) to 0.85 (sinapoylglucose). The standard errors of cross validation (SECV) ranged from 0.38 (other sinapate esters) to 0.70 mg/g seed (total sinapate esters). In validation and in independent validations the predicted results were not always acceptable, indicating that the NIRS calibrations need to be extended by analysing samples from new populations. Following replicated. field experiments, a doubled haploid line obtained from the old Dutch cultivar Mansholts' Hamburger Raps, and related DH lines from the cross DH Mansholts' x Express were confirmed to have a 30-40% lower sinapate ester content compared to check cultivars."],["dc.identifier.doi","10.1111/j.1439-0523.2007.01342.x"],["dc.identifier.isi","000246367400011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51313"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0179-9541"],["dc.title","Genetic variation for sinapate ester content in winter rapeseed (Brassica napus L.) and development of NIRS calibration equations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]