Becker, Heiko C.

[["dc.bibliographiccitation.firstpage","94"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Agricultural and Food Chemistry"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Niewitetzki, Oliver"],["dc.contributor.author","Tillmann, Peter"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:46:47Z"],["dc.date.available","2018-11-07T08:46:47Z"],["dc.date.issued","2010"],["dc.description.abstract","The development of oilseed rape cultivars with a high content of oleic acid (18: 1) and a low content of linolenic acid (18:3) in the seed oil is an important breeding aim. Oil of this quality is increasingly being sought by the food and the oleochemical industry. Since the oil quality is determined by the genotype of the seed, a selection can be performed among single seeds of segregating populations. For this purpose a high-throughput Near-Infrared Reflectance Spectroscopy (NIRS) method using an automated sample presentation unit for single seeds of oilseed rape and a spectrometer equipped with a photodiode array detector was developed. Single-seed analyses have been accomplished with a throughput of up to 800 seeds per hour. Seeds from segregating populations of different origin were analyzed by NIRS and gas chromatography. Calibration equations were developed and validated applying the Modified Partial Least Square regression (MPLS) and LOCAL procedure. In three independent validations, standard errors of prediction corrected for bias between 2.7% and 3.7% for oleic acid and 1.2% and 1.8% for linolenic acid were determined using MPLS. Similar results were obtained applying the LOCAL procedure. The results show that the new high-throughput method can be applied to predict the oleic acid and linolenic acid content of single seeds of oilseed rape."],["dc.identifier.doi","10.1021/jf9028199"],["dc.identifier.isi","000273268100012"],["dc.identifier.pmid","19961144"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7278"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20778"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0021-8561"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","A New Near-Infrared Reflectance Spectroscopy Method for High-Throughput Analysis of Oleic Acid and Linolenic Acid Content of Single Seeds in Oilseed Rape (Brassica napus L.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1051"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","1061"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Amar, Samija"],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:15:53Z"],["dc.date.available","2018-11-07T11:15:53Z"],["dc.date.issued","2008"],["dc.description.abstract","Improving oil and protein quality for food and feed purposes is an important goal in rapeseed (Brassica napus L.) breeding programs. Rapeseed contains phytosterols, used to enrich food products, and sinapate esters, which are limiting the utilization of rapeseed proteins in the feed industry. Increasing the phytosterol content of oil and lowering sinapate ester content of meal could increase the value of the oilseed rape crop. The objective of the present study was to identify quantitative trait loci (QTL) for phytosterol and sinapate ester content in a winter rapeseed population of 148 doubled haploid lines, previously found to have a large variation for these two traits. This population also segregated for the two erucic acid genes. A close negative correlation was found between erucic acid and phytosterol content (Spearman's rank correlation, r(s) = -0.80 ). For total phytosterol content, three QTL were detected, explaining 60% of the genetic variance. The two QTL with the strongest additive effects were mapped on linkage groups N8 and N13 within the confidence intervals of the two erucic acid genes. For sinapate ester content four QTL were detected, explaining 53% of the genetic variance. Again, a close negative correlation was found between erucic acid and sinapate ester content (r(s) = -0.66 ) and the QTL with the strongest additive effects mapped on linkage groups N8 and N13 within the confidence intervals of the two erucic acid genes. The results suggests, that there is a pleiotropic effect of the two erucic acid genes on phytosterol and sinapate ester content; the effect of the alleles for low erucic acid content is to increase phytosterol and sinapate ester content. Possible reasons for this are discussed based on known biosynthetic pathways."],["dc.identifier.doi","10.1007/s00122-008-0734-2"],["dc.identifier.isi","000256354300002"],["dc.identifier.pmid","18335203"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3475"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54469"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0040-5752"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","QTL for phytosterol and sinapate ester content in Brassica napus L. collocate with the two erucic acid genes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","765"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","773"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Nath, Ujjal K."],["dc.contributor.author","Wilmer, Jeroen A."],["dc.contributor.author","Wallington, Emma J."],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:33:21Z"],["dc.date.available","2018-11-07T08:33:21Z"],["dc.date.issued","2009"],["dc.description.abstract","High erucic acid rapeseed (HEAR) oil is of interest for industrial purposes because erucic acid (22:1) and its derivatives are important renewable raw materials for the oleochemical industry. Currently available cultivars contain only about 50% erucic acid in the seed oil. A substantial increase in erucic acid content would significantly reduce processing costs and could increase market prospects of HEAR oil. It has been proposed that erucic acid content in rapeseed is limited because of insufficient fatty acid elongation, lack of insertion of erucic acid into the central sn-2 position of the triaclyglycerol backbone and due to competitive desaturation of the precursor oleic acid (18:1) to linoleic acid (18:2). The objective of the present study was to increase erucic content of HEAR winter rapeseed through over expression of the rapeseed fatty acid elongase gene (fae1) in combination with expression of the lysophosphatidic acid acyltransferase gene from Limnanthes douglasii (Ld-LPAAT), which enables insertion of erucic acid into the sn-2 glycerol position. Furthermore, mutant alleles for low contents of polyunsaturated fatty acids (18:2 + 18:3) were combined with the transgenic material. Selected transgenic lines showed up to 63% erucic acid in the seed oil in comparison to a mean of 54% erucic acid of segregating non-transgenic HEAR plants. Amongst 220 F(2) plants derived from the cross between a transgenic HEAR line and a non-transgenic HEAR line with a low content of polyunsaturated fatty acids, recombinant F(2) plants were identified with an erucic acid content of up to 72% and a polyunsaturated fatty acid content as low as 6%. Regression analysis revealed that a reduction of 10% in polyunsaturated fatty acids content led to a 6.5% increase in erucic acid content. Results from selected F(2) plants were confirmed in the next generation by analysing F(4) seeds harvested from five F(3) plants per selected F(2) plant. F(3) lines contained up to 72% erucic acid and as little as 4% polyunsaturated fatty acids content in the seed oil. The 72% erucic acid content of rapeseed oil achieved in the present study represents a major breakthrough in breeding high erucic acid rapeseed."],["dc.description.sponsorship","German Academic Exchange Service (DAAD), Bonn, Germany"],["dc.identifier.doi","10.1007/s00122-008-0936-7"],["dc.identifier.isi","000263391800012"],["dc.identifier.pmid","19050848"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3476"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17554"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0040-5752"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT plus Bn-fae1 transgenes in rapeseed (Brassica napus L.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","125"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Breeding"],["dc.bibliographiccitation.lastpage","138"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Nath, Ujjal K."],["dc.contributor.author","Goswami, Gayatri"],["dc.contributor.author","Clemens, Rosemarie"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:35:07Z"],["dc.date.available","2018-11-07T08:35:07Z"],["dc.date.issued","2009"],["dc.description.abstract","Erucic acid ( 22: 1) is a valuable renewable resource for the oleochemical industry. Currently available high erucic acid rapeseed cultivars contain only about 50% erucic acid in the seed oil. A substantial increase of the erucic acid content of the rapeseed oil could increase market prospects. The transgenic line TNKAT, over expressing the rapeseed fatty acid elongase gene (fae1) and expressing the Ld-LPAAT gene from Limnanthes douglasii was crossed with the line 6575-1 HELP ( high erucic and low polyunsaturated fatty acid). A from the F(1) plants produced population of 90 doubled haploid (DH) lines was tested in a greenhouse with three replicates. Parental lines TNKAT and 6575-1 HELP contained 46 and 50% erucic acid in the seed oil, respectively. In the DH population the erucic acid content ranged between 35 and 59%. The Ld-LPAAT + Bn-fae1.1 transgene showed a 1: 1 segregation. The transgenic DH lines contained up to 8% trierucolyglycerol, but surprisingly had a by 2.3% lower erucic acid content compared to the non-transgenic segregants. Results indicated that the ectopically expressed fae1.1 gene may not be functional. The DH population also showed a large quantitative variation for PUFA content ranging from 6 to 28% ( TNKAT: 21%, 6575-1 HELP: 8%). Regression analysis showed that in the DH population a 10% reduction in PUFA content led to a 4.2% increase in erucic acid content. Development of locus specific PCR primers for the two resident erucic acid genes fae1.1 ( A-genome) and fae1.2 genes (C-genome) of rapeseed allowed sequencing of the respective alleles from TNKAT and 6575-1 HELP. Single nucleotide polymorphisms were only found for the fae1.1 gene. Use of allele specific fae1.1 PCR primers, however, did not reveal a significant effect of the fae1.1 allele from either parent on erucic acid content. The high erucic acid low polyunsaturated fatty acid DH lines and the fae1 locus specific primers developed in the present study should be useful in future studies aimed at increasing erucic acid content in rapeseed."],["dc.identifier.doi","10.1007/s11032-008-9220-4"],["dc.identifier.isi","000262268700011"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3595"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17986"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1380-3743"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Inheritance and variation of erucic acid content in a transgenic rapeseed (Brassica napus L.) doubled haploid population"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]