Becker, Heiko C.

[["dc.bibliographiccitation.firstpage","94"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Agricultural and Food Chemistry"],["dc.bibliographiccitation.lastpage","100"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Niewitetzki, Oliver"],["dc.contributor.author","Tillmann, Peter"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:46:47Z"],["dc.date.available","2018-11-07T08:46:47Z"],["dc.date.issued","2010"],["dc.description.abstract","The development of oilseed rape cultivars with a high content of oleic acid (18: 1) and a low content of linolenic acid (18:3) in the seed oil is an important breeding aim. Oil of this quality is increasingly being sought by the food and the oleochemical industry. Since the oil quality is determined by the genotype of the seed, a selection can be performed among single seeds of segregating populations. For this purpose a high-throughput Near-Infrared Reflectance Spectroscopy (NIRS) method using an automated sample presentation unit for single seeds of oilseed rape and a spectrometer equipped with a photodiode array detector was developed. Single-seed analyses have been accomplished with a throughput of up to 800 seeds per hour. Seeds from segregating populations of different origin were analyzed by NIRS and gas chromatography. Calibration equations were developed and validated applying the Modified Partial Least Square regression (MPLS) and LOCAL procedure. In three independent validations, standard errors of prediction corrected for bias between 2.7% and 3.7% for oleic acid and 1.2% and 1.8% for linolenic acid were determined using MPLS. Similar results were obtained applying the LOCAL procedure. The results show that the new high-throughput method can be applied to predict the oleic acid and linolenic acid content of single seeds of oilseed rape."],["dc.identifier.doi","10.1021/jf9028199"],["dc.identifier.isi","000273268100012"],["dc.identifier.pmid","19961144"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7278"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20778"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","0021-8561"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","A New Near-Infrared Reflectance Spectroscopy Method for High-Throughput Analysis of Oleic Acid and Linolenic Acid Content of Single Seeds in Oilseed Rape (Brassica napus L.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2195"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Crop Science"],["dc.bibliographiccitation.lastpage","2199"],["dc.bibliographiccitation.volume","46"],["dc.contributor.author","zum Felde, Thomas"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T09:18:08Z"],["dc.date.available","2018-11-07T09:18:08Z"],["dc.date.issued","2006"],["dc.description.abstract","Improving the meal and protein quality for feed and food purposes is of increasing importance in canola (Brassica napus L.). The phenolic acid ester content contributes to the bitter taste, astringency, and dark color of rapeseed meal products. The predominant phenolic acid esters are sinapate esters (SE), which make up 1 to 2% of the seed dry matter. The objective of the present study was to analyze the genetic variation and the genotype X environment interactions for SE content and composition in three populations of doubled haploid lines. The populations were grown in three to four environments in Germany. The following SE were analyzed by HPLC: sinapoylcholine (sinapine), sinapoylglucose, and a minor group of other SE which includes sinapate. The three populations showed a highly significant variation for the total SE content, and sinapine was the predominant sinapate ester compound. The analysis of variance showed highly significant effects for the genotype (G), the environment (E) and the G X E interactions for all three populations. In two of the populations the G X E interaction variance components were less than half of the genetic variance, in one population it was slightly higher. The estimates for heritability of the individual and total SE were generally high and ranged from 0.57 to 0.93. A reduction of sinapate ester content was not associated with a change in oil, protein, and glucosinolate content."],["dc.identifier.doi","10.2135/cropsci2006.03.0155"],["dc.identifier.isi","000240821800045"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28335"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Crop Science Soc Amer"],["dc.relation.issn","0011-183X"],["dc.title","Genotype x environment interactions, heritability, and trait correlations of sinapate ester content in winter rapeseed (Brassica napus L.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1051"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","1061"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Amar, Samija"],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:15:53Z"],["dc.date.available","2018-11-07T11:15:53Z"],["dc.date.issued","2008"],["dc.description.abstract","Improving oil and protein quality for food and feed purposes is an important goal in rapeseed (Brassica napus L.) breeding programs. Rapeseed contains phytosterols, used to enrich food products, and sinapate esters, which are limiting the utilization of rapeseed proteins in the feed industry. Increasing the phytosterol content of oil and lowering sinapate ester content of meal could increase the value of the oilseed rape crop. The objective of the present study was to identify quantitative trait loci (QTL) for phytosterol and sinapate ester content in a winter rapeseed population of 148 doubled haploid lines, previously found to have a large variation for these two traits. This population also segregated for the two erucic acid genes. A close negative correlation was found between erucic acid and phytosterol content (Spearman's rank correlation, r(s) = -0.80 ). For total phytosterol content, three QTL were detected, explaining 60% of the genetic variance. The two QTL with the strongest additive effects were mapped on linkage groups N8 and N13 within the confidence intervals of the two erucic acid genes. For sinapate ester content four QTL were detected, explaining 53% of the genetic variance. Again, a close negative correlation was found between erucic acid and sinapate ester content (r(s) = -0.66 ) and the QTL with the strongest additive effects mapped on linkage groups N8 and N13 within the confidence intervals of the two erucic acid genes. The results suggests, that there is a pleiotropic effect of the two erucic acid genes on phytosterol and sinapate ester content; the effect of the alleles for low erucic acid content is to increase phytosterol and sinapate ester content. Possible reasons for this are discussed based on known biosynthetic pathways."],["dc.identifier.doi","10.1007/s00122-008-0734-2"],["dc.identifier.isi","000256354300002"],["dc.identifier.pmid","18335203"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3475"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54469"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0040-5752"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","QTL for phytosterol and sinapate ester content in Brassica napus L. collocate with the two erucic acid genes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","115"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Breeding"],["dc.bibliographiccitation.lastpage","125"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Zhao, Jianyi"],["dc.contributor.author","Dimov, Zoran"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:20:23Z"],["dc.date.available","2018-11-07T11:20:23Z"],["dc.date.issued","2008"],["dc.description.abstract","Increasing oil content and improving the fatty acid composition in the seed oil are important breeding goals for rapeseed (Brassica napus L.). The objective of the study was to investigate a possible relationship between fatty acid composition and oil content in an oilseed rape doubled haploid (DH) population. The DH population was derived from a cross between the German cultivar Sollux and the Chinese cultivar Gaoyou, both having a high erucic acid and a very high oil content. In total, 282 DH lines were evaluated in replicated field experiments in four environments, two each in Germany and in China. Fatty acid composition of the seed oil was analyzed by gas liquid chromatography and oil content was determined by NIRS. Quantitative trait loci (QTL) for fatty acid contents were mapped and their additive main effects were determined by a mixed model approach using the program QTLMapper. For all fatty acids large and highly significant genetic variations among the genotypes were observed. High heritabilities were determined for oil content and for all fatty acids (h(2) = 0.82 to 0.94), except for stearic acid content (h(2) = 0.38). Significant correlations were found between the contents of all individual fatty acids and oil content. Closest genetic correlations were found between oil content and the sum of polyunsaturated fatty acids (18:2 + 18:3; r(G) = -0.46), the sum of monounsaturated fatty acids (18:1 + 20:1 + 22:1; r(G) = 0.46) and palmitic acid (16:0; r(G) = -0.34), respectively. Between one and eight QTL for the contents of the different fatty acids were detected. Together, their additive main effects explained between 28% and 65% of the genetic variance for the individual fatty acids. Ten QTL for fatty acid contents mapped within a distance of 0 to 10 cM to QTL for oil content, which were previously identified in this DH population. QTL mapped within this distance to each other are likely to be identical. The results indicate a close interrelationship between fatty acid composition and oil content, which should be considered when breeding for increased oil content or improved oil composition in rapeseed."],["dc.identifier.doi","10.1007/s11032-007-9113-y"],["dc.identifier.isi","000251321400010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55523"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1572-9788"],["dc.relation.issn","1380-3743"],["dc.title","Mapping QTL controlling fatty acid composition in a doubled haploid rapeseed population segregating for oil content"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2405"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","2415"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Schatzki, Joerg"],["dc.contributor.author","Schoo, Burkhard"],["dc.contributor.author","Ecke, Wolfgang"],["dc.contributor.author","Herrfurth, Cornelia"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T09:20:52Z"],["dc.date.available","2018-11-07T09:20:52Z"],["dc.date.issued","2013"],["dc.description.abstract","Following winter oilseed rape cultivation, considerable numbers of volunteer oilseed rape plants may occur in subsequent years in following crops. The appearance of volunteer oilseed rape plants is based on the capability of the seeds to become secondary dormant and to survive in this stage for many years in the soil. Genetic reduction of secondary seed dormancy in oilseed rape could provide a means to reduce the frequency of volunteer plants and especially the dispersal of transgenic oilseed rape. The objective of the present study was to analyse the inheritance of primary and secondary seed dormancy in a winter oilseed rape doubled haploid population derived from the cross Express 617 x R53 and to study correlations to other seed traits. Field experiments were performed in Germany for 2 years at two locations with two replicates. Seeds harvested from open pollinated plants were used for all analyses, including a laboratory test for seed dormancy. A previously developed molecular marker map of the doubled haploid population was used to map QTL of the relevant traits. For primary, secondary and total seed dormancy, the results showed significant effects of the genotypes and their interactions, with years and locations. Two, four and five QTL were detected for primary, secondary and total seed dormancy which explained 19, 35 and 42 % of the phenotypic variance, respectively. Results show that secondary seed dormancy is a heritable trait and that selection for low secondary seed dormancy is possible."],["dc.description.sponsorship","German Federal Ministry of Education and Research (BMBF) [FKZ 0315211C]"],["dc.identifier.doi","10.1007/s00122-013-2144-3"],["dc.identifier.isi","000323671100017"],["dc.identifier.pmid","23783224"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28977"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0040-5752"],["dc.title","Mapping of QTL for seed dormancy in a winter oilseed rape doubled haploid population"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","27"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Euphytica"],["dc.bibliographiccitation.lastpage","34"],["dc.bibliographiccitation.volume","153"],["dc.contributor.author","Hom, Nang Hseng"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:06:31Z"],["dc.date.available","2018-11-07T11:06:31Z"],["dc.date.issued","2007"],["dc.description.abstract","Near infrared-reflectance spectroscopy (NIRS) is widely used as an efficient and non-destructive method for seed quality analysis in oilseed rape (Brassica napus L.). Normally, calibration equations are developed for seed samples sizes of 3-4 g. However, during the breeding process much less than 3 g of samples are frequently only available for analysis. The objective of the present study was to analyse whether calibration equations developed for standard sample sizes can be used to predict the oil, protein, moisture, glucosinolate and fatty acid content of sample sizes ranging from about 450 to 5 mg (single seeds). Special adapters were made for the standard ring cups to hold the smaller samples during NIRS scanning. Close Spearman rank correlations (r(S) = 0.82-1.00 ) showed that NIRS calibration equations developed for standard small ring cups can be used to predict the oil, protein, and glucosinolate content of smaller samples of 450-100 mg seeds. For oleic, linolenic and erucic acid, close correlations were only found for adapter sizes holding 450-250 mg seeds (r (S) = 0.79-0.98 ). Only moderate to very low correlations were found for the moisture content. The standard NIRS calibration equations could also be used to predict the oil content (R-2 = 0.79 ) and the protein content (R-2 = 0.72 ) of single seeds (ca. 5 mg), whereas this was not possible for the glucosinolate content (R-2 = 0.54 ). Therefore, single seed NIRS calibration equations were developed for alkenyl, indole and total glucosinolate content. The coefficients of determinations in cross validation ranged from 0.83 (alkenyl) to 0.86 (indole and total glucosinolates)."],["dc.identifier.doi","10.1007/s10681-006-9195-3"],["dc.identifier.isi","000242852500004"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52332"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0014-2336"],["dc.title","Non-destructive analysis of rapeseed quality by NIRS of small seed samples and single seeds"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","493"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Crop Science"],["dc.bibliographiccitation.lastpage","500"],["dc.bibliographiccitation.volume","55"],["dc.contributor.author","Hom, Nang Hseng"],["dc.contributor.author","Schierholt, Antje"],["dc.contributor.author","Moellers, Christian"],["dc.contributor.author","Becker, Heiko C."],["dc.date.accessioned","2018-11-07T10:00:05Z"],["dc.date.available","2018-11-07T10:00:05Z"],["dc.date.issued","2015"],["dc.description.abstract","The genetics of seed compounds is complex because seed quality traits might be exclusively determined by the genotype of the female parent or, depending on the genotype of the embryo, may also be affected by the genotype of the pollen parent. For many quality traits of oilseed rape (Brassica napus L.), the influence of the pollen parent is not well known. In this study, two male sterile lines (female parent) were pollinated by 10 genotypes (pollen parent) differing largely in seed quality. Field experiments were conducted at three locations in Northern Germany, with two replications in 1 yr. Pollen parents were sown in plots and 10 male sterile plants from each female parent were hand planted into the center of each plot. Seeds were harvested from pollen parents and from the interplanted female parents. Seeds were analyzed for oil, protein, and sinapic acid esters by near-infrared reflectance spectroscopy; for glucosinolate and tocopherol content by high-performance liquid chromatography; and for fatty acid content by gas liquid chromatography. Earlier results were confirmed in that fatty acid composition is dependent on the embryo genotype and is therefore largely influenced by the pollen parent. Significant pollen parent effects were also observed for oil content, indole glucosinolate, sinapoylglucose, total sinapic acid ester, and gamma-tocopherol contents. The F-1 seed quality was positively correlated with the pollen parent seed quality. In conclusion, not only the fatty acid composition but also other seed quality traits such as oil content are influenced by the genotype of the pollen parent. This should be considered when analyzing open pollinated plants."],["dc.identifier.doi","10.2135/cropsci2014.04.0284"],["dc.identifier.isi","000352223000003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37723"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Crop Science Soc Amer"],["dc.relation.issn","1435-0653"],["dc.relation.issn","0011-183X"],["dc.title","Pollen Genotype Effects on Seed Quality Traits in Winter Oilseed Rape"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1000"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Crop Science"],["dc.bibliographiccitation.lastpage","1006"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Amar, Samija"],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T11:15:26Z"],["dc.date.available","2018-11-07T11:15:26Z"],["dc.date.issued","2008"],["dc.description.abstract","Phytosterols are natural constituents of vegetable oils and are known for their cholesterol-lowering properties. The oil of rapeseed (Brassica napus L.) is one of the richest natural sources of phytosterols. Genetically enhancing the phytosterol content could give an added value to the rapeseed oil and derived products. Our objectives were to develop a gas-liquid chromatographic method for the analysis of phytosterol content in seeds of oilseed rape, to determine the genetic variation and the genotype x environment interactions, and to estimate correlations between phytosterols and other important seed quality traits in three doubled haploid populations of winter rapeseed. The populations were tested during several years in three to four environments. Sitosterol and campesterol were detected as the two major phytosterols followed by brassicasterol, avenasterol, and stigmasterol. Large differences were found in total phytosterol content (2.57 to 4.15 g kg(-1) seed), with predominant genetic variance components resulting in high heritabilities ranging from 0.84 to 0.91. Phytosterol content was not negatively correlated with oil content and there were no close correlations to protein and glucosinolate content. The large genetic variation along with high heritabilities indicate that an effective breeding for enhanced phytosterol content and modified composition should be possible without negative impacts on oil, protein, or glucosinolate content."],["dc.identifier.doi","10.2135/cropsci2007.10.0578"],["dc.identifier.isi","000256635400017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54364"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Crop Science Soc Amer"],["dc.relation.issn","0011-183X"],["dc.title","Genetic variation and genotype x environment interactions of phytosterol content in three doubled haploid populations of winter rapeseed"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","78"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Plant Breeding"],["dc.bibliographiccitation.lastpage","83"],["dc.bibliographiccitation.volume","128"],["dc.contributor.author","Amar, Samija"],["dc.contributor.author","Becker, H. C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:33:18Z"],["dc.date.available","2018-11-07T08:33:18Z"],["dc.date.issued","2009"],["dc.description.abstract","Rapeseed oil is one of the richest natural sources of phytosterols, known to reduce the LDL-cholesterol levels, one of the major cardiovascular disease risk factors. Increasing the phytosterol content in rapeseed could give an added value to the oil and derived products. Our objective was to analyse the genetic variation of phytosterol content in modern winter rapeseed cultivars and resynthesized lines following field experiments and to develop a near-infrared reflectance spectroscopic (NIRS) calibration for high throughput estimation of phytosterol content. Phytosterol content as analysed by gas-liquid chromatography ranged from 3565 to 4800 mg/kg seed for modern cultivars and from 2079 to 4329 mg/kg seed for resynthesized lines. The NIRS calibration showed a high fraction of explained variance in cross-validation of 0.81 for total phytosterol content and the standard error of cross-validation was 241 mg/kg. The results show that the cultivars contain already high phytosterol contents. The NIRS calibrations developed for total phytosterol content should be useful for germplasm screening and in breeding programmes aimed at increasing the phytosterol content in rapeseed."],["dc.description.sponsorship","Hans-Bockler-Stiftung"],["dc.identifier.doi","10.1111/j.1439-0523.2008.01531.x"],["dc.identifier.isi","000262830600010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17543"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0179-9541"],["dc.title","Genetic variation in phytosterol content of winter rapeseed (Brassica napus L.) and development of NIRS calibration equations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","765"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Theoretical and Applied Genetics"],["dc.bibliographiccitation.lastpage","773"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Nath, Ujjal K."],["dc.contributor.author","Wilmer, Jeroen A."],["dc.contributor.author","Wallington, Emma J."],["dc.contributor.author","Becker, Heiko C."],["dc.contributor.author","Moellers, Christian"],["dc.date.accessioned","2018-11-07T08:33:21Z"],["dc.date.available","2018-11-07T08:33:21Z"],["dc.date.issued","2009"],["dc.description.abstract","High erucic acid rapeseed (HEAR) oil is of interest for industrial purposes because erucic acid (22:1) and its derivatives are important renewable raw materials for the oleochemical industry. Currently available cultivars contain only about 50% erucic acid in the seed oil. A substantial increase in erucic acid content would significantly reduce processing costs and could increase market prospects of HEAR oil. It has been proposed that erucic acid content in rapeseed is limited because of insufficient fatty acid elongation, lack of insertion of erucic acid into the central sn-2 position of the triaclyglycerol backbone and due to competitive desaturation of the precursor oleic acid (18:1) to linoleic acid (18:2). The objective of the present study was to increase erucic content of HEAR winter rapeseed through over expression of the rapeseed fatty acid elongase gene (fae1) in combination with expression of the lysophosphatidic acid acyltransferase gene from Limnanthes douglasii (Ld-LPAAT), which enables insertion of erucic acid into the sn-2 glycerol position. Furthermore, mutant alleles for low contents of polyunsaturated fatty acids (18:2 + 18:3) were combined with the transgenic material. Selected transgenic lines showed up to 63% erucic acid in the seed oil in comparison to a mean of 54% erucic acid of segregating non-transgenic HEAR plants. Amongst 220 F(2) plants derived from the cross between a transgenic HEAR line and a non-transgenic HEAR line with a low content of polyunsaturated fatty acids, recombinant F(2) plants were identified with an erucic acid content of up to 72% and a polyunsaturated fatty acid content as low as 6%. Regression analysis revealed that a reduction of 10% in polyunsaturated fatty acids content led to a 6.5% increase in erucic acid content. Results from selected F(2) plants were confirmed in the next generation by analysing F(4) seeds harvested from five F(3) plants per selected F(2) plant. F(3) lines contained up to 72% erucic acid and as little as 4% polyunsaturated fatty acids content in the seed oil. The 72% erucic acid content of rapeseed oil achieved in the present study represents a major breakthrough in breeding high erucic acid rapeseed."],["dc.description.sponsorship","German Academic Exchange Service (DAAD), Bonn, Germany"],["dc.identifier.doi","10.1007/s00122-008-0936-7"],["dc.identifier.isi","000263391800012"],["dc.identifier.pmid","19050848"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3476"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17554"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0040-5752"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT plus Bn-fae1 transgenes in rapeseed (Brassica napus L.)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]