Alves, Frauke

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","02"],["dc.bibliographiccitation.journal","Journal of Medical Imaging"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Khan, Amara"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-03-10T15:14:49Z"],["dc.date.available","2020-03-10T15:14:49Z"],["dc.date.issued","2020"],["dc.description.abstract","Purpose: We present a phase-contrast x-ray tomography study of wild type C57BL/6 mouse hearts as a nondestructive approach to the microanatomy on the scale of the entire excised organ. Based on the partial coherence at a home-built phase-contrast μ-CT setup installed at a liquid metal jet source, we exploit phase retrieval and hence achieve superior image quality for heart tissue, almost comparable to previous synchrotron data on the whole organ scale. Approach: In our work, different embedding methods and heavy metal-based stains have been explored. From the tomographic reconstructions, quantitative structural parameters describing the three-dimensional (3-D) architecture have been derived by two different fiber tracking algorithms. The first algorithm is based on the local gradient of the reconstructed electron density. By performing a principal component analysis on the local structure-tensor of small subvolumes, the dominant direction inside the volume can be determined. In addition to this approach, which is already well established for heart tissue, we have implemented and tested an algorithm that is based on a local 3-D Fourier transform. Results: We showed that the choice of sample preparation influences the 3-D structure of the tissue, not only in terms of contrast but also with respect to the structural preservation. A heart prepared with the evaporation-of-solvent method was used to compare both algorithms. The results of structural orientation were very similar for both approaches. In addition to the determination of the fiber orientation, the degree of filament alignment and local thickness of single muscle fiber bundles were obtained using the Fourier-based approach. Conclusions: Phase-contrast x-ray tomography allows for investigating the structure of heart tissue with an isotropic resolution below 10  μm. The fact that this is possible with compact laboratory instrumentation opens up new opportunities for screening samples and optimizing sample preparation, also prior to synchrotron beamtimes. Further, results from the structural analysis can help in understanding cardiovascular diseases or can be used to improve computational models of the heart."],["dc.identifier.doi","10.1117/1.JMI.7.2.023501"],["dc.identifier.pmid","32206684"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63285"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/194"],["dc.language.iso","en"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","2329-4302"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Alves (Translationale Molekulare Bildgebung)"],["dc.subject.gro","biomedical tomography"],["dc.title","Fiber orientation in a whole mouse heart reconstructed by laboratory phase-contrast micro-CT"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4"],["dc.contributor.author","Nicolas, J. D."],["dc.contributor.author","Markus, Marietta Andrea"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Töpperwien, Mareike"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.editor","Wang, Geng"],["dc.contributor.editor","Müller-Myhsok, Bertram"],["dc.date.accessioned","2020-06-26T10:11:47Z"],["dc.date.available","2020-06-26T10:11:47Z"],["dc.date.issued","2017"],["dc.description.abstract","In this work we present x-ray phase-contrast tomography of heart tissue from mouse, combining computed tomography (CT) scans with laboratory and synchrotron radiation. The work serves as a proof-of-concept that the cyto-architecture and in particular the myofibril orientation can be assessed in three dimensions (3D) by phase-contrast CT. We demonstrate the synergistic use of laboratory μ -CT and of the high resolution synchrotron setup based on waveguide optics. Details on preparation, instrumentation and analysis are given, as a state of the art reference for heart tissue tomography, and as a starting point for further progress."],["dc.identifier.doi","10.1117/12.2276648"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/66750"],["dc.language.iso","en"],["dc.notes.preprint","yes"],["dc.relation.eventend","2017-09-25"],["dc.relation.eventlocation","San Diego"],["dc.relation.eventstart","2017-09-25"],["dc.relation.isbn","9781510612396"],["dc.relation.isbn","9781510612402"],["dc.relation.iserratumof","yes"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.subject.gro","x-ray optics"],["dc.subject.gro","x-ray imaging"],["dc.subject.gro","biomedical tomography"],["dc.title","Nanoscale holographic tomography of heart tissue with x-ray waveguide optics"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","2633"],["dc.bibliographiccitation.firstpage","2633"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Biomedical Optics Express"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Khan, Amara"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Salditt, Tim"],["dc.date.accessioned","2020-12-10T18:42:01Z"],["dc.date.available","2020-12-10T18:42:01Z"],["dc.date.issued","2020"],["dc.description.abstract","The spatial organization of cardiac muscle tissue exhibits a complex structure on multiple length scales, from the sarcomeric unit to the whole organ. Here we demonstrate a multi-scale three-dimensional imaging (3d) approach with three levels of magnification, based on synchrotron X-ray phase contrast tomography. Whole mouse hearts are scanned in an undulator beam, which is first focused and then broadened by divergence. Regions-of-interest of the hearts are scanned in parallel beam as well as a biopsy by magnified cone beam geometry using a X-ray waveguide optic. Data is analyzed in terms of orientation, anisotropy and the sarcomeric periodicity via a local Fourier transformation."],["dc.identifier.doi","10.1364/BOE.386576"],["dc.identifier.pmid","32499949"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17770"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77772"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/192"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","2156-7085"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Alves (Translationale Molekulare Bildgebung)"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.rights","Goescholar"],["dc.rights.uri","https://goedoc.uni-goettingen.de/licenses"],["dc.subject.gro","biomedical tomography"],["dc.title","Multi-scale X-ray phase-contrast tomography of murine heart tissue"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L763"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","American Journal of Physiology. Lung Cellular and Molecular Physiology"],["dc.bibliographiccitation.lastpage","L771"],["dc.bibliographiccitation.volume","313"],["dc.contributor.author","Markus, M. Andrea"],["dc.contributor.author","Borowik, Sergej"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Tromba, Giuliana"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Dullin, Christian"],["dc.date.accessioned","2018-10-10T11:58:28Z"],["dc.date.available","2018-10-10T11:58:28Z"],["dc.date.issued","2017-11-01"],["dc.description.abstract","Chronic asthma patients experience difficulties even years after the inciting allergen. Although studies in small animal asthma models have enormously advanced progress in uncovering the mechanisms of inception and development of the disease, little is known about the processes involved in the persistence of asthma symptoms in the absence of allergen exposure. Long-term asthma mouse models have so far been scarce or not been able to reproduce the findings in patients. Here we used a common ovalbumin-induced acute allergic airway inflammation mouse model to study lung function and remodeling after a 4-mo recovery period. We show by X-ray-based lung function measurements that the recovered mice continue to show impaired lung function by displaying significant air trapping compared with controls. High-resolution synchrotron phase-contrast computed tomography of structural alterations and diaphragm motion analysis suggest that these changes in pulmonary function are the result of a pronounced loss in lung elasticity. Histology of lung sections confirmed that this is most likely caused by a decrease in elastic fibers, indicating that remodeling can develop or persist independent of acute inflammation and is closely related to a loss in lung function. Our findings demonstrate that this X-ray-based imaging platform has the potential to comprehensively and noninvasively unravel long-term effects in preclinical mouse models of allergic airway inflammation and thus benefits our understanding of chronic asthma."],["dc.identifier.doi","10.1152/ajplung.00136.2017"],["dc.identifier.gro","631972"],["dc.identifier.pmid","28775094"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15962"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1522-1504"],["dc.title","X-ray-based lung function measurement reveals persistent loss of lung tissue elasticity in mice recovered from allergic airway inflammation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1707"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Synchrotron Radiation"],["dc.bibliographiccitation.lastpage","1719"],["dc.bibliographiccitation.volume","27"],["dc.contributor.affiliation","Frohn, Jasper; 1Institute for X-ray Physics, Universität Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Pinkert-Leetsch, Diana; 2Institute of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert Koch Strasse 40, 37075Göttingen, Germany"],["dc.contributor.affiliation","Missbach-Güntner, Jeannine; 2Institute of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert Koch Strasse 40, 37075Göttingen, Germany"],["dc.contributor.affiliation","Reichardt, Marius; 1Institute for X-ray Physics, Universität Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Osterhoff, Markus; 1Institute for X-ray Physics, Universität Göttingen, Friedrich-Hund-Platz 1, 37077Göttingen, Germany"],["dc.contributor.affiliation","Alves, Frauke; 2Institute of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Robert Koch Strasse 40, 37075Göttingen, Germany"],["dc.contributor.author","Frohn, Jasper"],["dc.contributor.author","Reichardt, Marius"],["dc.contributor.author","Osterhoff, Markus"],["dc.contributor.author","Salditt, Tim"],["dc.contributor.author","Pinkert-Leetsch, Diana"],["dc.contributor.author","Missbach-Güntner, Jeannine"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2020-12-03T08:19:06Z"],["dc.date.available","2020-12-03T08:19:06Z"],["dc.date.issued","2020-10-26"],["dc.date.updated","2022-02-09T13:21:33Z"],["dc.description.abstract","A multiscale three-dimensional (3D) virtual histology approach is presented, based on two configurations of propagation phase-contrast X-ray tomography, which have been implemented in close proximity at the GINIX endstation at the beamline P10/PETRA III (DESY, Hamburg, Germany). This enables the 3D reconstruction of characteristic morphological features of human pancreatic normal and tumor tissue, as obtained from cancer surgery, first in the form of a large-scale overview by parallel-beam illumination, followed by a zoom into a region-of-interest based on zoom tomography using a Kirkpatrick-Baez mirror with additional waveguide optics. To this end 1 mm punch biopsies of the tissue were taken. In the parallel tomography, a volumetric throughput on the order of 0.01 mm3 s-1 was achieved, while maintaining the ability to segment isolated cells. With a continuous rotation during the scan, a total acquisition time of less than 2 min was required for a full tomographic scan. Using the combination of both setups, islets of Langerhans, a three-dimensional cluster of cells in the endocrine part of the pancreas, could be located. Cells in such an islet were segmented and visualized in 3D. Further, morphological alterations of tumorous tissue of the pancreas were characterized. To this end, the anisotropy parameter Ω, based on intensity gradients, was used in order to quantify the presence of collagen fibers within the entire biopsy specimen. This proof-of-concept experiment of the multiscale approach on human pancreatic tissue paves the way for future 3D virtual pathology."],["dc.description.abstract","This paper presents propagation‐based phase‐contrast tomography in two configurations at the beamline endstation GINIX, demonstrated on the application of 1 mm human pancreatic tumor tissue biopsies. image"],["dc.identifier.doi","10.1107/S1600577520011327"],["dc.identifier.pmid","33147198"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/69419"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/85"],["dc.language.iso","en"],["dc.notes.intern","DeepGreen Import"],["dc.publisher","International Union of Crystallography"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","1600-5775"],["dc.relation.orgunit","Institut für Röntgenphysik"],["dc.relation.workinggroup","RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics)"],["dc.relation.workinggroup","RG Alves (Translationale Molekulare Bildgebung)"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use,\r\n distribution and reproduction in any medium, provided the original work is properly cited."],["dc.subject.gro","biomedical tomography"],["dc.title","3D virtual histology of human pancreatic tissue by multiscale phase-contrast X-ray tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]