Georges, Léa E.

[["dc.bibliographiccitation.firstpage","3224"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Journal of Archaeological Science"],["dc.bibliographiccitation.lastpage","3229"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","Seidenberg, Verena"],["dc.contributor.author","Schilz, Felix"],["dc.contributor.author","Pfister, Daniela"],["dc.contributor.author","Georges, Lea E."],["dc.contributor.author","Fehren-Schmitz, Lars"],["dc.contributor.author","Hummel, Susanne"],["dc.date.accessioned","2018-11-07T09:05:33Z"],["dc.date.available","2018-11-07T09:05:33Z"],["dc.date.issued","2012"],["dc.description.abstract","The analysis of short tandem repeats (STRs) is a useful tool in various contexts of ancient DNA research. Main applications are the reconstruction of kinship, identification, and authentication. Here we describe a short amplicon autosomal short tandem repeat (miniSTR) heptaplex system for the amplification of D13S317, D21S11, D18S51, TH01, D5S818, FGA and Amelogenin from highly degraded DNA as an inexpensive alternative to commercially available kits. All primers were newly designed and the amplicon length of all systems is less than 200 bp, with the exception of some rare alleles in the FGA and D21S11 systems. To validate the suitability of this system for typing STRs from human specimens with low DNA preservation we systematically tested it on 20 skeletal samples from four archaeological sites representing different burial environments and time spans since death. Finally, to test the sensitivity of the heptaplex system, we analyzed serial dilutions of control DNA and ancient DNA extracts. Using the system we were able to reproducibly obtain full STR profiles, down to a concentration of 0.06 ng DNA. Even with 0.004 ng DNA partial profiles could be amplified. The accumulated power of discrimination for the six selected STR loci is 0.99999984, plus the option of genetic sex determination through Amelogenin. The tests conducted prove that the system presented is efficient and especially suited for cases where STRs have to be typed and sex has to be assessed from human specimens with highly degraded DNA. (C) 2012 Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.jas.2012.05.019"],["dc.identifier.isi","000308452600016"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25349"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Ltd- Elsevier Science Ltd"],["dc.relation.issn","0305-4403"],["dc.title","A new miniSTR heptaplex system for genetic fingerprinting of ancient DNA from archaeological human bone"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","242"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","American Journal of Physical Anthropology"],["dc.bibliographiccitation.lastpage","249"],["dc.bibliographiccitation.volume","149"],["dc.contributor.author","Georges, Lea E."],["dc.contributor.author","Seidenberg, Verena"],["dc.contributor.author","Hummel, Susanne"],["dc.contributor.author","Fehren-Schmitz, Lars"],["dc.date.accessioned","2018-11-07T09:05:19Z"],["dc.date.available","2018-11-07T09:05:19Z"],["dc.date.issued","2012"],["dc.description.abstract","The majority of Native Americans nearly exclusively belong to group O of the ABO blood group system. Several hypotheses have been formulated to explain this observation, primarily differing by the presumption that the observed patterns of ABO diversity are due to the processes of the initial peopling of the Americas or due to subsequent events, especially the demographic consequences in the wake of European contact. A promising strategy to reveal possible diachronic ABO frequency changes is the molecular genetic analysis of relevant genetic markers in precontact populations. A previous study by Halverson and Bolnick [Am J Phys Anthropol 137 (2008) 342-347] already accomplished this for indigenous North American populations. Here we present the first study to analyze ABO blood types from pre-Columbian individuals from South America using molecular genetic methods and comparing them to several extant South American, North American, and Siberian populations. We tried to determine ABO blood types for 59 individuals from the southern Peruvian highlands dating to similar to 650 to 1250 AD using a newly developed multiplex PCR/SBE assay coamplifying the fragments relevant for blood type determination and three highly discriminating autosomal STRs. Analysis was successful for 31 individuals and revealed that all are exclusively in the O group, predominantly carrying the O02 (01v) allele. No significant difference could be observed between the ancient and modern Native American populations, while all significantly differed from the extant Siberian populations, supporting the suggestion that low ABO diversity results from founder effects during the initial peopling of the Americas. Am J Phys Anthropol 149:242249, 2012. (c) 2012 Wiley Periodicals, Inc."],["dc.description.sponsorship","German Research Foundation (DFG) [FE1161/1-1]"],["dc.identifier.doi","10.1002/ajpa.22115"],["dc.identifier.isi","000308879100009"],["dc.identifier.pmid","22806956"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25288"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0002-9483"],["dc.title","Molecular characterization of ABO blood group frequencies in pre-Columbian Peruvian highlanders"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]