Smit, Inga

[["dc.bibliographiccitation.firstpage","261"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Food Technology and Biotechnology"],["dc.bibliographiccitation.lastpage","268"],["dc.bibliographiccitation.volume","53"],["dc.contributor.author","Trümper, Christina"],["dc.contributor.author","Paffenholz, Katrin"],["dc.contributor.author","Smit, Inga"],["dc.contributor.author","Kössler, Philip"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Braun, Hans Peter"],["dc.contributor.author","Pawelzik, Elke"],["dc.date.accessioned","2018-09-10T08:34:51Z"],["dc.date.available","2018-09-10T08:34:51Z"],["dc.date.issued","2015"],["dc.description.abstract","This study was conducted to improve the knowledge of molecular processes involved in the interaction between Fusarium graminearum and emmer in the course of grain ripening. Emmer plants were artificially inoculated with a F. graminearum spore suspension at anthesis. In the course of grain ripening from milk ripe to plant death stage, grains at four phenological growth stages were collected for analysis. The infection degree was evaluated based on the F. graminearum DNA content in emmer grain infolding tissues (glumes and rachis). For proteome analysis the albumin and globulin fractions of emmer grains, consisting of proteins with various functions related to the development and stress response, were analysed regarding the changes due to Fusarium infection by two-dimensional gel electrophoresis. Altogether, forty-three proteins affected by infection were identified by mass spectrometry. Enzymes detoxifying reactive oxygen species were regulated at all developmental stages. In the early stage of grain development, the abundance of proteins related to stress response, such as 2-Cys peroxiredoxin, a chitinase, a xylanase inhibitor and a spermidine synthase was increased. During later stage of grain development, the abundance of stress-related proteins, such as chitinases, heat shock proteins and an α-amylase inhibitor-like protein, decreased. During all ripening stages, but especially during medium milk stage (BBCH 75) and soft dough stage (BBCH 85), the abundance of proteins related to carbon metabolism, starch and protein biosynthesis as well as photosynthesis increased due to F. graminearum infection. At the plant death stage (BBCH 97) the abundance of only two proteins related to metabolism decreased."],["dc.identifier.doi","10.17113/ftb.53.03.15.3838"],["dc.identifier.pmid","27904357"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15682"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Identification of Differently Regulated Proteins after
Fusarium graminearum Infection of Emmer (Triticum dicoccum) at Several Grain Ripening Stages"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","86"],["dc.bibliographiccitation.journal","Journal of Proteomics"],["dc.bibliographiccitation.lastpage","92"],["dc.bibliographiccitation.volume","133"],["dc.contributor.author","Trümper, Christina"],["dc.contributor.author","Paffenholz, Katrin"],["dc.contributor.author","Smit, Inga"],["dc.contributor.author","Kössler, Philip"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Braun, Hans-Peter"],["dc.contributor.author","Pawelzik, Elke"],["dc.date.accessioned","2018-09-10T08:36:13Z"],["dc.date.available","2018-09-10T08:36:13Z"],["dc.date.issued","2016"],["dc.description.abstract","We analyzed the effect of Fusarium graminearum infection on field-grown naked barley (Hordeum vulgare nudum). The ears were inoculated with F. graminearum spores during anthesis. In the course of ripening, grains in five phenological growth stages of naked barley from milk ripe to plant death were sampled. The albumin and globulin proteins of inoculated grains and untreated (control) grains were separated by two-dimensional gel electrophoresis. Forty-five spots composing of proteins that were changed in abundance due to F. graminearum infection were subsequently identified by mass spectrometry. Various proteins showing altered expression pattern after Fusarium infection were linked to stress response such as plant signal transduction pathways, fungal defense and oxidative burst. More proteins changed during early grain ripening stages than during later ripening stages. Protease inhibitors occurred at increased abundancy during milk ripe stage. A thaumatin-like protein accumulated at plant death stage. Proteins linked to nitrogen metabolism and protein biosynthesis were mainly reduced, whereas those linked to carbon metabolism were predominantly increased in infected grains."],["dc.identifier.doi","10.1016/j.jprot.2015.11.015"],["dc.identifier.pmid","26612662"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15683"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.issn","1876-7737"],["dc.title","Identification of regulated proteins in naked barley grains (Hordeum vulgare nudum) after Fusarium graminearum infection at different grain ripening stages"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","197"],["dc.bibliographiccitation.journal","Agriculture, Ecosystems & Environment"],["dc.bibliographiccitation.lastpage","204"],["dc.bibliographiccitation.volume","258"],["dc.contributor.author","Wietzke, Alexander"],["dc.contributor.author","Westphal, Catrin"],["dc.contributor.author","Gras, Pierre"],["dc.contributor.author","Kraft, Manuel"],["dc.contributor.author","Pfohl, Katharina"],["dc.contributor.author","Karlovsky, Petr"],["dc.contributor.author","Pawelzik, Elke"],["dc.contributor.author","Tscharntke, Teja"],["dc.contributor.author","Smit, Inga"],["dc.date.accessioned","2019-07-23T08:25:43Z"],["dc.date.available","2019-07-23T08:25:43Z"],["dc.date.issued","2018"],["dc.description.abstract","About 35% of global crop production arises from crop species that benefit from animal pollination, especially by insects. Animal pollination can enhance yields and increase fruit quality, but the effects of insect pollination on pre- and post-harvest fruit physiology and quality are largely unknown. For the first time, we analysed in much detail the physiological responses of fruit development and marketable quality improvements to different pollination treatments such as self-pollination, open-pollination and hand-pollination. In strawberries, self-pollination led to reduced seed set (fertilized achenes), reduced concentration of the phytohormone auxin, highest share of deformed fruits (> 90%), smallest and lightest fruits, considerably lower commercial value (8% of the value of open- or hand-pollinated fruits), and reduced shelf life of fruits. Overall, insect pollination increased the average commercial value of marketable fruits by 92%. The commercial value of hand-pollinated and open-pollinated strawberries did not differ. We conclude that pollination services are not merely important for yield, but also vital for physiological processes that result in better marketable quality (e.g. fruit appearance, flavour-enhancing constituents, prolonged shelf life) and commercial value of many pollinator-dependent crops."],["dc.identifier.doi","10.1016/j.agee.2018.01.036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61888"],["dc.language.iso","en"],["dc.relation.issn","0167-8809"],["dc.title","Insect pollination as a key factor for strawberry physiology and marketable fruit quality"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]