Wemheuer, Franziska

[["dc.bibliographiccitation.artnumber","14183"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Vidal, Stefan"],["dc.date.accessioned","2020-03-12T08:51:55Z"],["dc.date.available","2020-03-12T08:51:55Z"],["dc.date.issued","2019"],["dc.description.abstract","Green islands (the re-greening of senescent leaf tissues) are particularly evident on leaves infected with fungal pathogens. To date, there is only a limited number of studies investigating foliar endophytic microorganisms in phytopathogen-infected leaves. Here, we analysed bacterial and fungal endophyte communities in leaves without green islands (control leaves; CL), within green island areas (GLA) and the surrounding yellow leaf areas (YLA) of leaves with green islands of Acer campestre and A. platanoides. GLA samples of A. campestre and A. platanoides were dominated by Sawadaea polyfida and S. bicornis, respectively, suggesting that these fungi might be responsible for the green islands. We detected a higher fungal richness and diversity in CL compared to GLA samples of A. campestre. Leaf status (CL, GLA, YLA) significantly altered the composition of fungal communities of A. campestre. This was related to differences in fungal community composition between YLA and GLA samples. Site was the main driver of bacterial communities, suggesting that bacterial and fungal endophytes are shaped by different factors. Overall, we observed Acer species-specific responses of endophyte communities towards the presence of green islands and/or leaf type, which might be attributed to several fungi and bacteria specifically associated with one Acer species."],["dc.identifier.doi","10.1038/s41598-019-50540-2"],["dc.identifier.pmid","31578453"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16477"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63324"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.eissn","2045-2322"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Deciphering bacterial and fungal endophyte communities in leaves of two maple trees with green islands"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Liesegang, Heiko"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Wemheuer, Bernd"],["dc.date.accessioned","2020-04-28T12:41:35Z"],["dc.date.available","2020-04-28T12:41:35Z"],["dc.date.issued","2018"],["dc.description.abstract","Bacillus mycoides GM6LP is an endophyte isolated from plant tissues of Lolium perenne L. Here, we report its draft genome sequence (6.2 Mb), which contains 96 contigs and 6,129 protein-coding genes. Knowledge about its genome will enable us to evaluate the potential use of GM6LP as a plant growth-promoting bacterium."],["dc.identifier.doi","10.1128/genomeA.00011-18"],["dc.identifier.eissn","2169-8287"],["dc.identifier.pmid","29437086"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64449"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","2169-8287"],["dc.title","Draft Genome Sequence of the Endophyte Bacillus mycoides Strain GM6LP Isolated from Lolium perenne"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","13"],["dc.bibliographiccitation.lastpage","21"],["dc.bibliographiccitation.seriesnr","2555"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Pfeiffer, Birgit"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.editor","Streit, Wolfgang R."],["dc.contributor.editor","Daniel, Rolf"],["dc.date.accessioned","2022-11-01T10:17:28Z"],["dc.date.available","2022-11-01T10:17:28Z"],["dc.date.issued","2023"],["dc.identifier.doi","10.1007/978-1-0716-2795-2_2"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116819"],["dc.notes.intern","DOI-Import GROB-605"],["dc.publisher","Springer US"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-0716-2795-2"],["dc.relation.isbn","978-1-0716-2794-5"],["dc.relation.ispartof","Metagenomics : Methods and Protocols"],["dc.title","Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Granzow, Sandra"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Vidal, Stefan"],["dc.contributor.author","Wemheuer, Franziska"],["dc.date.accessioned","2020-12-10T18:37:01Z"],["dc.date.available","2020-12-10T18:37:01Z"],["dc.date.issued","2018"],["dc.description.abstract","Paenibacillus amylolyticus strain GM1FR is an endophyte isolated from aerial plant tissues of Festuca rubra L. Here, we report the draft genome sequence (7.3 Mb) of GM1FR containing 6,241 protein-coding genes, some of which are potentially involved in plant growth promotion and biocontrol."],["dc.identifier.doi","10.1128/genomeA.01516-17"],["dc.identifier.eissn","2169-8287"],["dc.identifier.pmid","29371353"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76813"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","2169-8287"],["dc.title","First Insights into the Draft Genome Sequence of the Endophyte Paenibacillus amylolyticus Strain GM1FR, Isolated from Festuca rubra L."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Genome Announcements"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Hollensteiner, Jacqueline"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Granzow, Sandra"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Vidal, Stefan"],["dc.contributor.author","Wemheuer, Bernd"],["dc.date.accessioned","2020-12-10T18:36:53Z"],["dc.date.available","2020-12-10T18:36:53Z"],["dc.date.issued","2017"],["dc.description.abstract","Pseudomonas putida GM4FR is an endophytic bacterium isolated from aerial plant tissues of Festuca rubra L. Functional annotation of the draft genome (7.1 Mb) revealed 6,272 predicted protein-encoding genes. The genome provides insights into the biocontrol and plant growth-promoting potential of P. putida GM4FR."],["dc.identifier.doi","10.1128/genomeA.00086-17"],["dc.identifier.eissn","2169-8287"],["dc.identifier.pmid","28360162"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76772"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","2169-8287"],["dc.title","Draft Genome Sequence of Pseudomonas putida Strain GM4FR, an Endophytic Bacterium Isolated from Festuca rubra L"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","UNSP 695826"],["dc.bibliographiccitation.journal","Archaea"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2018-11-07T09:14:45Z"],["dc.date.available","2018-11-07T09:14:45Z"],["dc.date.issued","2012"],["dc.description.abstract","Archaea play an important role in various biogeochemical cycles. They are known extremophiles inhabiting environments such as thermal springs or hydrothermal vents. Recent studies have revealed a significant abundance of Archaea in moderate environments, for example, temperate sea water. Nevertheless, the composition and ecosystem function of these marine archaeal communities is largely unknown. To assess diversity and composition of active archaeal communities in the German Bight, seven marine water samples were taken and studied by RNA-based analysis of ribosomal 16S rRNA. For this purpose, total RNA was extracted from the samples and converted to cDNA. Archaeal community structures were investigated by pyrosequencing-based analysis of 16S rRNA amplicons generated from cDNA. To our knowledge, this is the first study combining next-generation sequencing and metatranscriptomics to study archaeal communities in marine habitats. The pyrosequencing-derived dataset comprised 62,045 archaeal 16S rRNA sequences. We identified Halobacteria as the predominant archaeal group across all samples with increased abundance in algal blooms. Thermoplasmatales (Euryarchaeota) and the Marine Group I (Thaumarchaeota) were identified in minor abundances. It is indicated that archaeal community patterns were influenced by environmental conditions."],["dc.identifier.doi","10.1155/2012/695826"],["dc.identifier.isi","000311574700001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8395"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27493"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1472-3646"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","RNA-Based Assessment of Diversity and Composition of Active Archaeal Communities in the German Bight"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","323"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Letters in Applied Microbiology"],["dc.bibliographiccitation.lastpage","329"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Wemheuer, F."],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Kretzschmar, D."],["dc.contributor.author","Pfeiffer, B."],["dc.contributor.author","Herzog, Sven"],["dc.contributor.author","Daniel, Roy Thomas"],["dc.contributor.author","Vidal, Stefan"],["dc.date.accessioned","2018-11-07T10:16:27Z"],["dc.date.available","2018-11-07T10:16:27Z"],["dc.date.issued","2016"],["dc.description.abstract","Most plant species are colonized by endophytic bacteria. Despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. Hence, we investigated the bacterial community structure in three agricultural important grass species Dactylis glomerata L., Festuca rubra L. and Lolium perenne L. with regard to fertilizer application and different mowing frequencies. For this purpose, above-ground plant material was collected from the Grassland Management Experiment (GrassMan) in Germany in September 2010 and 2011. DNA was extracted from surface-sterilized plant tissue and subjected to 16S rRNA gene PCRs. Endophytic community structures were assessed by denaturing gradient gel electrophoresis (DGGE)-based analysis of obtained PCR products. DGGE fingerprints revealed that fertilizer application significantly altered the endophytic communities in L.perenne and F.rubra but not in D.glomerata. Although no direct effect of mowing was observed, mowing frequencies in combination with fertilizer application had a significant impact on endophyte bacterial community structures. However, this effect was not observed for all three grass species in both years. Therefore, our results showed that management regimes changed the bacterial endophyte communities, but this effect was plant-specific and varied over time. Significance and Impact of the StudyEndophytic bacteria play an important role in plant health and growth. However, studies addressing the influence of grassland management regimes on these bacteria in above-ground plant parts are still missing. In this study, we present first evidence that fertilizer application significantly impacted bacterial community structures in three agricultural important grass species, whereas mowing had only a minor effect. Moreover, this effect was plant-specific and thus not visible for all grass species in each year. Consequently, this study sheds new light into the complex interaction of microbes and plants."],["dc.identifier.doi","10.1111/lam.12551"],["dc.identifier.isi","000373000600005"],["dc.identifier.pmid","26834040"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41042"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1472-765X"],["dc.relation.issn","0266-8254"],["dc.title","Impact of grassland management regimes on bacterial endophyte diversity differs with grass species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","405"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Berkelmann, Dirk"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Vidal, Stefan"],["dc.contributor.author","Bisseleua Daghela, Hervé Bertin"],["dc.date.accessioned","2020-04-28T12:39:40Z"],["dc.date.available","2020-04-28T12:39:40Z"],["dc.date.issued","2020"],["dc.description.abstract","Cacao (Theobroma cacao L.) is one of the most economically important crops worldwide. Despite the important role of endophytes for plant growth and health, very little is known about the effect of agroforestry management systems on the endophyte communities of T.cacao. To close this knowledge gap, we investigated the diversity, community composition, and function of bacterial and fungal endophytes in the leaves of T.cacao trees growing in five major cacao-growing regions in the central region of Cameroon using DNA metabarcoding. Fungal but not bacterial alpha diversity measures differed significantly between the agroforestry management systems. Interestingly, less managed home-garden cacao forests harbored the lowest fungal richness and diversity. Our results suggest that the composition of bacterial and fungal endophyte communities is predominantly affected by agroforestry management systems and, to a lesser extent, by environmental properties. The core microbiome detected comprised important fungal phytopathogens, such as Lasiodiplodia species. Several predicted pathways of bacterial endophytes and functional guilds of fungal endophytes differed between the agroforest systems which might be attributed to bacteria and fungi specifically associated with a single agroforest. Our results provide the basis for future studies on foliar fungal and bacterial endophytes of T.cacao and their responsiveness towards agroforestry management systems."],["dc.description.sponsorship","Volkswagen Foundation"],["dc.identifier.doi","10.3390/microorganisms8030405"],["dc.identifier.pmid","32183118"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17357"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64434"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","MDPI"],["dc.relation.eissn","2076-2607"],["dc.relation.issn","2076-2607"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Agroforestry Management Systems Drive the Composition, Diversity, and Function of Fungal and Bacterial Endophyte Communities in Theobroma Cacao Leaves"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","136714"],["dc.bibliographiccitation.journal","Archaea"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.author","Taube, Robert"],["dc.contributor.author","Akyol, Pinar"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Daniel, Rolf"],["dc.date.accessioned","2018-11-07T09:30:11Z"],["dc.date.available","2018-11-07T09:30:11Z"],["dc.date.issued","2013"],["dc.description.abstract","Volcanic regions contain a variety of environments suitable for extremophiles. This study was focused on assessing and exploiting the prokaryotic diversity of two microbial communities derived from different Kamchatkian thermal springs by metagenomic approaches. Samples were taken from a thermoacidophilic spring near the Mutnovsky Volcano and from a thermophilic spring in the Uzon Caldera. Environmental DNA for metagenomic analysis was isolated from collected sediment samples by direct cell lysis. The prokaryotic community composition was examined by analysis of archaeal and bacterial 16S rRNA genes. A total number of 1235 16S rRNA gene sequences were obtained and used for taxonomic classification. Most abundant in the samples were members of Thaumarchaeota, Thermotogae, and Proteobacteria. The Mutnovsky hot spring was dominated by the Terrestrial Hot Spring Group, Kosmotoga, and Acidithiobacillus. The Uzon Caldera was dominated by uncultured members of the Miscellaneous Crenarchaeotic Group and Enterobacteriaceae. The remaining 16S rRNA gene sequences belonged to the Aquificae, Dictyoglomi, Euryarchaeota, Korarchaeota, Thermodesulfobacteria, Firmicutes, and some potential new phyla. In addition, the recovered DNA was used for generation of metagenomic libraries, which were subsequently mined for genes encoding lipolytic and proteolytic enzymes. Three novel genes conferring lipolytic and one gene conferring proteolytic activity were identified."],["dc.description.sponsorship","Bundesministerium fur Bildung und Forschung (BMBF)"],["dc.identifier.doi","10.1155/2013/136714"],["dc.identifier.isi","000315875000001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9968"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31245"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1472-3646"],["dc.title","Microbial Diversity and Biochemical Potential Encoded by Thermal Spring Metagenomes Derived from the Kamchatka Peninsula"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","13"],["dc.bibliographiccitation.lastpage","22"],["dc.bibliographiccitation.volume","1539"],["dc.contributor.author","Schneider, Dominik"],["dc.contributor.author","Wemheuer, Franziska"],["dc.contributor.author","Pfeiffer, Birgit"],["dc.contributor.author","Wemheuer, Bernd"],["dc.contributor.editor","Streit, W."],["dc.contributor.editor","Daniel, R."],["dc.date.accessioned","2020-03-12T09:01:08Z"],["dc.date.available","2020-03-12T09:01:08Z"],["dc.date.issued","2017"],["dc.description.abstract","Microbial communities play an important role in marine ecosystem processes. Although the number of studies targeting marker genes such as the 16S rRNA gene has been increased in the last few years, the vast majority of marine diversity is rather unexplored. Moreover, most studies focused on the entire bacterial community and thus disregarded active microbial community players. Here, we describe a detailed protocol for the simultaneous extraction of DNA and RNA from marine water samples and for the generation of cDNA from the isolated RNA which can be used as a universal template in various marker gene studies."],["dc.identifier.doi","10.1007/978-1-4939-6691-2_2"],["dc.identifier.pmid","27900681"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63326"],["dc.language.iso","en"],["dc.publisher","Humana Press"],["dc.publisher.place","New York, NY"],["dc.relation.eisbn","978-1-4939-6691-2"],["dc.relation.eissn","1940-6029"],["dc.relation.isbn","978-1-4939-6689-9"],["dc.relation.ispartof","Metagenomics. Methods in Molecular Biology"],["dc.relation.issn","1064-3745"],["dc.title","Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a cDNA as Universal Template for Marker Gene Studies"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]