Jovin, Thomas M.

[["dc.bibliographiccitation.firstpage","131"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","FEBS Letters"],["dc.bibliographiccitation.lastpage","135"],["dc.bibliographiccitation.volume","479"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Subramaniam, Vinod"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Jovin, Thomas M."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:46Z"],["dc.date.available","2017-09-07T11:46:46Z"],["dc.date.issued","2000"],["dc.description.abstract","The green fluorescent protein (GFP) has become an invaluable marker for monitoring protein localization and gene expression in vivo. Recently a new red fluorescent protein (drFP583 or DsRed), isolated from tropical corals, has been described [Matz, M.V. et al, (1999) Nature Biotech, 17, 969-973]. With emission maxima at 509 and 583 nm respectively, EGFP and DsRed are suited for almost crossover free dual color labeling upon simultaneous excitation, We imaged mixed populations of Escherichia coli expressing either EGFP or DsRed by one-photon confocal and by two-photon microscopy, Both excitation modes proved to be suitable for imaging cells expressing either of the fluorescent proteins. DsRed had an extended maturation time and E, coli expressing this fluorescent protein were significantly smaller than those expressing EGFP, In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved."],["dc.identifier.doi","10.1016/S0014-5793(00)01896-2"],["dc.identifier.gro","3144363"],["dc.identifier.isi","000088963900011"],["dc.identifier.pmid","10981721"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1979"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0014-5793"],["dc.title","EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","271"],["dc.bibliographiccitation.lastpage","290"],["dc.contributor.author","Subramaniam, Vinod"],["dc.contributor.author","Kirsch, Achim K."],["dc.contributor.author","Jenei, Attila"],["dc.contributor.author","Jovin, Thomas M."],["dc.contributor.editor","Robinson, J. Paul"],["dc.contributor.editor","Babcock, George F."],["dc.contributor.editor","Durack, Gary"],["dc.contributor.editor","Robinson, J. Paul"],["dc.date.accessioned","2021-12-08T12:29:45Z"],["dc.date.available","2021-12-08T12:29:45Z"],["dc.date.issued","2000"],["dc.identifier.doi","10.1002/0471224847.ch12"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/96198"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-476"],["dc.publisher","John Wiley & Sons, Inc."],["dc.publisher.place","New York, USA"],["dc.relation.eisbn","0471224847"],["dc.relation.isbn","0471315753"],["dc.relation.ispartof","Cytometric Cellular Analysis"],["dc.relation.ispartof","Emerging Tools for Single-Cell Analysis"],["dc.title","Scanning Near-Field Optical Imaging and Spectroscopy in Cell Biology"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2039"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","EMBO Journal"],["dc.bibliographiccitation.lastpage","2046"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Fernandez, Claudio O."],["dc.contributor.author","Hoyer, W."],["dc.contributor.author","Zweckstetter, Markus"],["dc.contributor.author","Jares-Erijman, E. A."],["dc.contributor.author","Subramaniam, V."],["dc.contributor.author","Griesinger, Christian"],["dc.contributor.author","Jovin, Thomas M."],["dc.date.accessioned","2017-09-07T11:43:22Z"],["dc.date.available","2017-09-07T11:43:22Z"],["dc.date.issued","2004"],["dc.description.abstract","The aggregation of alpha-synuclein is characteristic of Parkinson's disease (PD) and other neurodegenerative synucleinopathies. The 140-aa protein is natively unstructured; thus, ligands binding to the monomeric form are of therapeutic interest. Biogenic polyamines promote the aggregation of alpha-synuclein and may constitute endogenous agents modulating the pathogenesis of PD. We characterized the complexes of natural and synthetic polyamines with alpha-synuclein by NMR and assigned the binding site to C-terminal residues 109-140. Dissociation constants were derived from chemical shift perturbations. Greater polyamine charge (+2 --> +5) correlated with increased affinity and enhancement of fibrillation, for which we propose a simple kinetic mechanism involving a dimeric nucleation center. According to the analysis, polyamines increase the extent of nucleation by similar to10(4) and the rate of monomer addition similar to40-fold. Significant secondary structure is not induced in monomeric alpha-synuclein by polyamines at 15degreesC. Instead, NMR reveals changes in a region (aa 22-93) far removed from the polyamine binding site and presumed to adopt the beta-sheet conformation characteristic of fibrillar alpha-synuclein. We conclude that the C-terminal domain acts as a regulator of alpha-synuclein aggregation."],["dc.identifier.doi","10.1038/sj.emboj.7600211"],["dc.identifier.gro","3143987"],["dc.identifier.isi","000221499800003"],["dc.identifier.pmid","15103328"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1561"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0261-4189"],["dc.title","NMR of alpha-synuclein-polyamine complexes elucidates the mechanism and kinetics of induced aggregation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","16233"],["dc.bibliographiccitation.issue","51"],["dc.bibliographiccitation.journal","Biochemistry"],["dc.bibliographiccitation.lastpage","16242"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Hoyer, Wolfgang"],["dc.contributor.author","Cherny, Dmitry"],["dc.contributor.author","Subramaniam, Vinod"],["dc.contributor.author","Jovin, Thomas M."],["dc.date.accessioned","2021-06-01T10:50:29Z"],["dc.date.available","2021-06-01T10:50:29Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1021/bi048453u"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86678"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1520-4995"],["dc.relation.issn","0006-2960"],["dc.title","Impact of the Acidic C-Terminal Region Comprising Amino Acids 109−140 on α-Synuclein Aggregation in Vitro †"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","3235"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","3240"],["dc.bibliographiccitation.volume","278"],["dc.contributor.author","Antony, Thomas"],["dc.contributor.author","Hoyer, Wolfgang"],["dc.contributor.author","Cherny, Dmitry"],["dc.contributor.author","Heim, Gudrun"],["dc.contributor.author","Jovin, Thomas M."],["dc.contributor.author","Subramaniam, Vinod"],["dc.date.accessioned","2021-06-01T10:51:07Z"],["dc.date.available","2021-06-01T10:51:07Z"],["dc.date.issued","2003"],["dc.identifier.doi","10.1074/jbc.M208249200"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86900"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0021-9258"],["dc.title","Cellular Polyamines Promote the Aggregation of α-Synuclein"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]