Stühmer, Walter

[["dc.bibliographiccitation.artnumber","4595"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Ramos-Gomes, Fernanda"],["dc.contributor.author","Bode, Julia"],["dc.contributor.author","Sukhanova, Alyona"],["dc.contributor.author","Bozrova, Svetlana V."],["dc.contributor.author","Saccomano, Mara"],["dc.contributor.author","Mitkovski, Miso"],["dc.contributor.author","Krueger, Julia Eva"],["dc.contributor.author","Wege, Anja K."],["dc.contributor.author","Stuehmer, Walter"],["dc.contributor.author","Samokhvalov, Pavel S."],["dc.contributor.author","Baty, Daniel"],["dc.contributor.author","Chames, Patrick"],["dc.contributor.author","Nabiev, Igor"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-09T11:45:17Z"],["dc.date.available","2019-07-09T11:45:17Z"],["dc.date.issued","2018"],["dc.description.abstract","Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5-10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody-QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis."],["dc.identifier.doi","10.1038/s41598-018-22973-8"],["dc.identifier.pmid","29545609"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59199"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/246479/EU//NAMDIATREAM"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]