Stühmer, Walter

[["dc.bibliographiccitation.artnumber","4595"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Ramos-Gomes, Fernanda"],["dc.contributor.author","Bode, Julia"],["dc.contributor.author","Sukhanova, Alyona"],["dc.contributor.author","Bozrova, Svetlana V."],["dc.contributor.author","Saccomano, Mara"],["dc.contributor.author","Mitkovski, Miso"],["dc.contributor.author","Krueger, Julia Eva"],["dc.contributor.author","Wege, Anja K."],["dc.contributor.author","Stuehmer, Walter"],["dc.contributor.author","Samokhvalov, Pavel S."],["dc.contributor.author","Baty, Daniel"],["dc.contributor.author","Chames, Patrick"],["dc.contributor.author","Nabiev, Igor"],["dc.contributor.author","Alves, Frauke"],["dc.date.accessioned","2019-07-09T11:45:17Z"],["dc.date.available","2019-07-09T11:45:17Z"],["dc.date.issued","2018"],["dc.description.abstract","Early detection of malignant tumours and, especially, micrometastases and disseminated tumour cells is still a challenge. In order to implement highly sensitive diagnostic tools we demonstrate the use of nanoprobes engineered from nanobodies (single-domain antibodies, sdAbs) and fluorescent quantum dots (QDs) for single- and two-photon detection and imaging of human micrometastases and disseminated tumour cells in ex vivo biological samples of breast and pancreatic metastatic tumour mouse models expressing human epidermal growth factor receptor 2 (HER2) or carcinoembryonic antigen (CEA). By staining thin (5-10 µm) paraffin and thick (50 µm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Compared to conventional fluorescently labelled antibodies the sdAb-HER2-QD and sdAb-CEA-QD nanoprobes are superior in detecting micrometastases in tissue sections by lower photobleaching and higher brightness of fluorescence signals ensuring much better discrimination of positive signals versus background. Very high two-photon absorption cross-sections of QDs and small size of the nanoprobes ensure efficient imaging of thick tissue sections unattainable with conventional fluorescent probes. The nanobody-QD probes will help to improve early cancer diagnosis and prognosis of progression by assessing metastasis."],["dc.identifier.doi","10.1038/s41598-018-22973-8"],["dc.identifier.pmid","29545609"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59199"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation","info:eu-repo/grantAgreement/EC/FP7/246479/EU//NAMDIATREAM"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Molecular Cancer"],["dc.bibliographiccitation.lastpage","16"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Agarwal, Jasmin R."],["dc.contributor.author","Griesinger, Frank"],["dc.contributor.author","Stühmer, Walter"],["dc.contributor.author","Pardo, Luis A."],["dc.date.accessioned","2019-07-09T11:52:50Z"],["dc.date.available","2019-07-09T11:52:50Z"],["dc.date.issued","2010"],["dc.description.abstract","Background: The voltage-gated potassium channel hEag1 (KV10.1) has been related to cancer biology. The physiological expression of the human channel is restricted to the brain but it is frequently and abundantly expressed in many solid tumors, thereby making it a promising target for a specific diagnosis and therapy. Because chronic lymphatic leukemia has been described not to express hEag1, it has been assumed that the channel is not expressed in hematopoietic neoplasms in general. Results: Here we show that this assumption is not correct, because the channel is up-regulated in myelodysplastic syndromes, chronic myeloid leukemia and almost half of the tested acute myeloid leukemias in a subtypedependent fashion. Most interestingly, channel expression strongly correlated with increasing age, higher relapse rates and a significantly shorter overall survival. Multivariate Cox regression analysis revealed hEag1 expression levels in AML as an independent predictive factor for reduced disease-free and overall survival; such an association had not been reported before. As a functional correlate, specific hEag1 blockade inhibited the proliferation and migration of several AML cell lines and primary cultured AML cells in vitro. Conclusion: Our observations implicate hEag1 as novel target for diagnostic, prognostic and/or therapeutic approaches in AML."],["dc.identifier.doi","10.1186/1476-4598-9-18"],["dc.identifier.fs","567913"],["dc.identifier.pmid","20105281"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6032"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60288"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","The potassium channel Ether à go-go is a novel prognostic factor with functional relevance in acute myeloid leukemia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]