Fornasiero, Eugenio Francesco

[["dc.bibliographiccitation.firstpage","3333"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Nature Protocols"],["dc.bibliographiccitation.lastpage","3365"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Mandad, Sunit"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.date.accessioned","2020-12-10T18:10:05Z"],["dc.date.available","2020-12-10T18:10:05Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41596-019-0222-y"],["dc.identifier.pmid","31685960"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73844"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/110"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/80"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.relation.workinggroup","RG Urlaub (Bioanalytische Massenspektrometrie)"],["dc.title","A mass spectrometry workflow for measuring protein turnover rates in vivo"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","eabn4437"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","Science Advances"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Kluever, Verena"],["dc.contributor.author","Russo, Belisa"],["dc.contributor.author","Mandad, Sunit"],["dc.contributor.author","Kumar, Nisha Hemandhar"],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Ori, Alessandro"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Schneider, Anja"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.date.accessioned","2022-06-01T09:39:33Z"],["dc.date.available","2022-06-01T09:39:33Z"],["dc.date.issued","2022"],["dc.description.abstract","Aging is a prominent risk factor for neurodegenerative disorders (NDDs); however, the molecular mechanisms rendering the aged brain particularly susceptible to neurodegeneration remain unclear. Here, we aim to determine the link between physiological aging and NDDs by exploring protein turnover using metabolic labeling and quantitative pulse-SILAC proteomics. By comparing protein lifetimes between physiologically aged and young adult mice, we found that in aged brains protein lifetimes are increased by ~20% and that aging affects distinct pathways linked to NDDs. Specifically, a set of neuroprotective proteins are longer-lived in aged brains, while some mitochondrial proteins linked to neurodegeneration are shorter-lived. Strikingly, we observed a previously unknown alteration in proteostasis that correlates to parsimonious turnover of proteins with high biosynthetic costs, revealing an overall metabolic adaptation that preludes neurodegeneration. Our findings suggest that future therapeutic paradigms, aimed at addressing these metabolic adaptations, might be able to delay NDD onset."],["dc.description.abstract","Physiological brain aging affects protein turnover, altering the stability of proteins linked to neurodegenerative diseases."],["dc.identifier.doi","10.1126/sciadv.abn4437"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/108506"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-572"],["dc.relation.eissn","2375-2548"],["dc.title","Protein lifetimes in aged brains reveal a proteostatic adaptation linking physiological aging to neurodegeneration"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2100387"],["dc.bibliographiccitation.journal","PROTEOMICS"],["dc.contributor.author","Li, Wenxue"],["dc.contributor.author","Salovska, Barbora"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Liu, Yansheng"],["dc.date.accessioned","2022-12-01T08:31:35Z"],["dc.date.available","2022-12-01T08:31:35Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1002/pmic.202100387"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118208"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.relation.eissn","1615-9861"],["dc.relation.issn","1615-9853"],["dc.title","Towards a hypothesis‐free understanding of how Phosphorylation dynamically impacts protein turnover"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","436"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","BioEssays"],["dc.bibliographiccitation.lastpage","451"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2018-11-07T09:59:15Z"],["dc.date.available","2018-11-07T09:59:15Z"],["dc.date.issued","2015"],["dc.description.abstract","The recent 2014 Nobel Prize in chemistry honored an era of discoveries and technical advancements in the field of super-resolution microscopy. However, the applications of diffraction-unlimited imaging in biology have a long road ahead and persistently engage scientists with new challenges. Some of the bottlenecks that restrain the dissemination of super-resolution techniques are tangible, and include the limited performance of affinity probes and the yet not capillary diffusion of imaging setups. Likewise, super-resolution microscopy has introduced new paradigms in the design of projects that require imaging with nanometer-resolution and in the interpretation of biological images. Besides structural or morphological characterization, super-resolution imaging is quickly expanding towards interaction mapping, multiple target detection and live imaging. Here we review the recent progress of biologists employing super-resolution imaging, some pitfalls, implications and new trends, with the purpose of animating the field and spurring future developments."],["dc.identifier.doi","10.1002/bies.201400170"],["dc.identifier.isi","000351546000013"],["dc.identifier.pmid","25581819"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37549"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1521-1878"],["dc.relation.issn","0265-9247"],["dc.title","Super-resolution imaging for cell biologists Concepts, applications, current challenges and developments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","111"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Developmental Cell"],["dc.bibliographiccitation.lastpage","124.e6"],["dc.bibliographiccitation.volume","56"],["dc.contributor.author","Wu, Chongde"],["dc.contributor.author","Ba, Qian"],["dc.contributor.author","Lu, Dayun"],["dc.contributor.author","Li, Wenxue"],["dc.contributor.author","Salovska, Barbora"],["dc.contributor.author","Hou, Pingfu"],["dc.contributor.author","Mueller, Torsten"],["dc.contributor.author","Rosenberger, George"],["dc.contributor.author","Gao, Erli"],["dc.contributor.author","Di, Yi"],["dc.contributor.author","Zhou, Hu"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Liu, Yansheng"],["dc.date.accessioned","2021-04-14T08:30:32Z"],["dc.date.available","2021-04-14T08:30:32Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.devcel.2020.10.025"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83276"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.issn","1534-5807"],["dc.title","Global and Site-Specific Effect of Phosphorylation on Protein Turnover"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","eaam7816"],["dc.bibliographiccitation.issue","419"],["dc.bibliographiccitation.journal","Science Translational Medicine"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Fard, Maryam K."],["dc.contributor.author","van der Meer, Franziska"],["dc.contributor.author","Sánchez, Paula"],["dc.contributor.author","Cantuti-Castelvetri, Ludovico"],["dc.contributor.author","Mandad, Sunit"],["dc.contributor.author","Jäkel, Sarah"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Schmitt, Sebastian"],["dc.contributor.author","Ehrlich, Marc"],["dc.contributor.author","Starost, Laura"],["dc.contributor.author","Kuhlmann, Tanja"],["dc.contributor.author","Sergiou, Christina"],["dc.contributor.author","Schultz, Verena"],["dc.contributor.author","Wrzos, Claudia"],["dc.contributor.author","Brück, Wolfgang"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Dimou, Leda"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Simons, Mikael"],["dc.date.accessioned","2020-12-10T18:36:46Z"],["dc.date.available","2020-12-10T18:36:46Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1126/scitranslmed.aam7816"],["dc.identifier.eissn","1946-6242"],["dc.identifier.issn","1946-6234"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/76735"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","BCAS1 expression defines a population of early myelinating oligodendrocytes in multiple sclerosis lesions"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Truckenbrodt, S."],["dc.contributor.author","Viplav, A."],["dc.contributor.author","Denker, A."],["dc.contributor.author","Vogts, A."],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2018-11-07T10:04:17Z"],["dc.date.available","2018-11-07T10:04:17Z"],["dc.date.issued","2015"],["dc.identifier.isi","000209928600314"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38661"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.publisher.place","Bethesda"],["dc.relation.issn","1939-4586"],["dc.relation.issn","1059-1524"],["dc.title","A cellular molecular timer measures synaptic vesicle use and prevents the participation of aged vesicles in synaptic transmission"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e2121040119"],["dc.bibliographiccitation.issue","33"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.volume","119"],["dc.contributor.author","Katsenelson, Maxim"],["dc.contributor.author","Shapira, Ilana"],["dc.contributor.author","Abbas, Eman"],["dc.contributor.author","Jevdokimenko, Kristina"],["dc.contributor.author","Styr, Boaz"],["dc.contributor.author","Ruggiero, Antonella"],["dc.contributor.author","Aïd, Saba"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Holzenberger, Martin"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Slutsky, Inna"],["dc.date.accessioned","2022-09-01T09:50:22Z"],["dc.date.available","2022-09-01T09:50:22Z"],["dc.date.issued","2022"],["dc.description.abstract","Regulation of firing rate homeostasis constitutes a fundamental property of central neural circuits. While intracellular Ca\n 2+\n has long been hypothesized to be a feedback control signal, the molecular machinery enabling a network-wide homeostatic response remains largely unknown. We show that deletion of insulin-like growth factor-1 receptor (IGF-1R) limits firing rate homeostasis in response to inactivity, without altering the distribution of baseline firing rates. The deficient firing rate homeostatic response was due to disruption of both postsynaptic and intrinsic plasticity. At the cellular level, we detected a fraction of IGF-1Rs in mitochondria, colocalized with the mitochondrial calcium uniporter complex (MCUc). IGF-1R deletion suppressed transcription of the MCUc members and burst-evoked mitochondrial Ca\n 2+\n (mitoCa\n 2+\n ) by weakening mitochondria-to-cytosol Ca\n 2+\n coupling. Overexpression of either mitochondria-targeted IGF-1R or MCUc in IGF-1R–deficient neurons was sufficient to rescue the deficits in burst-to-mitoCa\n 2+\n coupling and firing rate homeostasis. Our findings indicate that mitochondrial IGF-1R is a key regulator of the integrated homeostatic response by tuning the reliability of burst transfer by MCUc. Based on these results, we propose that MCUc acts as a homeostatic Ca\n 2+\n sensor. Faulty activation of MCUc may drive dysregulation of firing rate homeostasis in aging and in brain disorders associated with aberrant IGF-1R/MCUc signaling."],["dc.description.sponsorship"," EC | European Research Council 501100000781"],["dc.description.sponsorship"," Israel Science Foundation 501100003977"],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft 501100001659"],["dc.description.sponsorship"," Volkswagen Foundation 501100001663"],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft 501100001659"],["dc.description.sponsorship"," BIRAX 501100020633"],["dc.identifier.doi","10.1073/pnas.2121040119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/113688"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-597"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.title","IGF-1 receptor regulates upward firing rate homeostasis via the mitochondrial calcium uniporter"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Trends in Biochemical Sciences"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Savas, Jeffrey N."],["dc.date.accessioned","2022-10-04T10:21:29Z"],["dc.date.available","2022-10-04T10:21:29Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1016/j.tibs.2022.08.011"],["dc.identifier.pii","S0968000422002365"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114421"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.issn","0968-0004"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Determining and interpreting protein lifetimes in mammalian tissues"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S1568163721002129"],["dc.bibliographiccitation.firstpage","101465"],["dc.bibliographiccitation.journal","Ageing Research Reviews"],["dc.bibliographiccitation.volume","72"],["dc.contributor.author","Kluever, Verena"],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.date.accessioned","2021-10-01T09:57:26Z"],["dc.date.available","2021-10-01T09:57:26Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.arr.2021.101465"],["dc.identifier.pii","S1568163721002129"],["dc.identifier.pmid","34555542"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89841"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/135"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-469"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation.issn","1568-1637"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.title","Principles of brain aging: Status and challenges of modeling human molecular changes in mice"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]