Fornasiero, Eugenio Francesco

[["dc.bibliographiccitation.artnumber","5"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Molecular and Cell Biology"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Bonnin, Elisa A."],["dc.contributor.author","Fornasiero, Eugenio F."],["dc.contributor.author","Lange, Felix"],["dc.contributor.author","Turck, Christoph W."],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2021-04-14T08:29:56Z"],["dc.date.available","2021-04-14T08:29:56Z"],["dc.date.issued","2021"],["dc.date.updated","2022-07-29T12:07:12Z"],["dc.description.abstract","Abstract\r\n \r\n Background\r\n Most of the cells of the mammalian retina are terminally differentiated, and do not regenerate once fully developed. This implies that these cells have strict controls over their metabolic processes, including protein turnover. We report the use of metabolic labelling procedures and secondary ion mass spectrometry imaging to examine nitrogen turnover in retinal cells, with a focus on the outer nuclear layer, inner nuclear layer, and outer plexiform layer.\r\n \r\n \r\n Results\r\n We find that turnover can be observed in all cells imaged using NanoSIMS. However, the rate of turnover is not constant, but varies between different cellular types and cell regions. In the inner and outer nuclear layers, turnover rate is higher in the cytosol than in the nucleus of each cell. Turnover rates are also higher in the outer plexiform layer. An examination of retinal cells from mice that were isotopically labeled very early in embryonic development shows that proteins produced during this period can be found in all cells and cell regions up to 2 months after birth, even in regions of high turnover.\r\n \r\n \r\n Conclusions\r\n Our results indicate that turnover in retinal cells is a highly regulated process, with strict metabolic controls. We also observe that turnover is several-fold higher in the synaptic layer than in cell layers. Nevertheless, embryonic proteins can still be found in this layer 2 months after birth, suggesting that stable structures persist within the synapses, which remain to be determined."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.citation","BMC Molecular and Cell Biology. 2021 Jan 11;22(1):5"],["dc.identifier.doi","10.1186/s12860-020-00339-1"],["dc.identifier.pmid","33430763"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17722"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83043"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/121"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/90"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A05: Mitochondriale Heterogenität in Synapsen"],["dc.relation.eissn","2661-8850"],["dc.relation.orgunit","Institut für Neuro- und Sinnesphysiologie"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 4.0"],["dc.rights.holder","The Author(s)"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject","Secondary ion mass spectrometry"],["dc.subject","Imaging"],["dc.subject","Metabolic labelling"],["dc.subject","Protein turnover"],["dc.subject","Retina"],["dc.title","NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]