Christoph, Jan

[["dc.bibliographiccitation.firstpage","293"],["dc.bibliographiccitation.lastpage","302"],["dc.bibliographiccitation.seriesnr","1408"],["dc.contributor.author","Richter, Claudia"],["dc.contributor.author","Christoph, Jan"],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.contributor.author","Luther, Stefan"],["dc.contributor.editor","Kianianmomeni, Arash"],["dc.date.accessioned","2018-05-07T10:56:42Z"],["dc.date.available","2018-05-07T10:56:42Z"],["dc.date.issued","2016"],["dc.description.abstract","The control of spatiotemporal dynamics in biological systems is a fundamental problem in nonlinear sciences and has important applications in engineering and medicine. Optogenetic tools combined with advanced optical technologies provide unique opportunities to develop and validate novel approaches to control spatiotemporal complexity in neuronal and cardiac systems. Understanding of the mechanisms and instabilities underlying the onset, perpetuation, and control of cardiac arrhythmias will enable the development and translation of novel therapeutic approaches. Here we describe in detail the preparation and optical mapping of transgenic channelrhodopsin-2 (ChR2) mouse hearts, cardiac cell cultures, and the optical setup for photostimulation using digital light processing."],["dc.identifier.doi","10.1007/978-1-4939-3512-3_20"],["dc.identifier.pmid","26965131"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14612"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/155"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.publisher","Humana Press"],["dc.publisher.place","New York"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.doi","10.1007/978-1-4939-3512-3_20"],["dc.relation.eissn","1940-6029"],["dc.relation.isbn","978-1-4939-3512-3"],["dc.relation.isbn","978-1-4939-3510-9"],["dc.relation.isbn","978-1-4939-3512-3"],["dc.relation.ispartof","Optogenetics: Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 1408"],["dc.relation.issn","1940-6029"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias"],["dc.type","book_chapter"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.volume","119"],["dc.contributor.author","Lilienkamp, Thomas"],["dc.contributor.author","Christoph, Jan"],["dc.contributor.author","Parlitz, Ulrich"],["dc.date.accessioned","2020-12-10T18:25:43Z"],["dc.date.available","2020-12-10T18:25:43Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1103/PhysRevLett.119.054101"],["dc.identifier.eissn","1079-7114"],["dc.identifier.issn","0031-9007"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/75803"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Features of Chaotic Transients in Excitable Media Governed by Spiral and Scroll Waves"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","787627"],["dc.bibliographiccitation.journal","Frontiers in Cardiovascular Medicine"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Lebert, Jan"],["dc.contributor.author","Ravi, Namita"],["dc.contributor.author","Kensah, George"],["dc.contributor.author","Christoph, Jan"],["dc.date.accessioned","2022-07-01T07:35:28Z"],["dc.date.available","2022-07-01T07:35:28Z"],["dc.date.issued","2022"],["dc.description.abstract","Optical mapping of action potentials or calcium transients in contracting cardiac tissues are challenging because of the severe sensitivity of the measurements to motion. The measurements rely on the accurate numerical tracking and analysis of fluorescence changes emitted by the tissue as it moves, and inaccurate or no tracking can produce motion artifacts and lead to imprecise measurements that can prohibit the analysis of the data. Recently, it was demonstrated that numerical motion-tracking and -stabilization can effectively inhibit motion artifacts, allowing highly detailed simultaneous measurements of electrophysiological phenomena and tissue mechanics. However, the field of electromechanical optical mapping is still young and under development. To date, the technique is only used by a few laboratories, the processing of the video data is time-consuming and performed offline post-acquisition as it is associated with a considerable demand for computing power. In addition, a systematic review of numerical motion tracking algorithms applicable to optical mapping data is lacking. To address these issues, we evaluated 5 open-source numerical motion-tracking algorithms implemented on a graphics processing unit (GPU) and compared their performance when tracking and compensating motion and measuring optical traces in voltage- or calcium-sensitive optical mapping videos of contracting cardiac tissues. Using GPU-accelerated numerical motion tracking, the processing times necessary to analyze optical mapping videos become substantially reduced. We demonstrate that it is possible to track and stabilize motion and create motion-compensated optical maps in real-time with low-resolution (128 x 128 pixels) and high resolution (800 x 800 pixels) optical mapping videos acquired at 500 and 40 fps, respectively. We evaluated the tracking accuracies and motion-stabilization capabilities of the GPU-based algorithms on synthetic optical mapping videos, determined their sensitivity to fluorescence signals and noise, and demonstrate the efficacy of the Farnebäck algorithm with recordings of contracting human cardiac cell cultures and beating hearts from 3 different species (mouse, rabbit, pig) imaged with 4 different high-speed cameras. GPU-accelerated processing provides a substantial increase in processing speed, which could open the path for more widespread use of numerical motion tracking and stabilization algorithms during routine optical mapping studies."],["dc.identifier.doi","10.3389/fcvm.2022.787627"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112178"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation.eissn","2297-055X"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Real-Time Optical Mapping of Contracting Cardiac Tissues With GPU-Accelerated Numerical Motion Tracking"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","529"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Traffic"],["dc.bibliographiccitation.lastpage","543"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Signoret, N."],["dc.contributor.author","Christophe, T."],["dc.contributor.author","Oppermann, Martin"],["dc.contributor.author","Marsh, M."],["dc.date.accessioned","2018-11-07T10:47:59Z"],["dc.date.available","2018-11-07T10:47:59Z"],["dc.date.issued","2004"],["dc.description.abstract","Following agonist activation, the chemokine receptor CCR5 is internalised through clathrin-coated pits and delivered to recycling endosomes. Subsequently, ligand- free and resensitised receptors are recycled to the cell surface. Currently little is known of the mechanisms regulating resensitisation and recycling of this G-protein coupled receptor. Here we show that raising the pH of endocytic compartments, using bafilomycin A, monensin or NH(4)Cl, does not significantly affect CCR5 endocytosis, recycling or dephosphorylation. By contrast, these reagents inhibited recycling of another well-characterised G protein coupled receptor, the beta(2)-adrenergic receptor, following agonist-induced internalisation. CCR5-bound RANTES (CCL5) and MIP-1beta (CCL4) only exhibit pH-dependent dissociation at pH < 4.0, below the values normally found in endocytic organelles. Although receptor-agonist dissociation is not dependent on low pH, the subsequent degradation of released chemokine is inhibited in the presence of reagents that raise endosomal pH. Our data show that exposure to low pH is not required for RANTES or MIP-1beta dissociation from CCR5, or for recycling of internalised CCR5 to the cell surface."],["dc.identifier.doi","10.1111/j.1600-0854.2004.00200.x"],["dc.identifier.isi","000221853800007"],["dc.identifier.pmid","15180829"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48091"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1398-9219"],["dc.title","PH-independent endocytic cycling of the chemokine receptor CCR5"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","50"],["dc.bibliographiccitation.journal","European Journal of Heart Failure. Supplements"],["dc.bibliographiccitation.lastpage","51"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","POST, H"],["dc.contributor.author","SCHMITTO, J"],["dc.contributor.author","STEENDIJK, P"],["dc.contributor.author","CHRISTOPH, J"],["dc.contributor.author","HOLLAND, R"],["dc.contributor.author","WACHTER, R"],["dc.contributor.author","SCHOENDUBE, F"],["dc.contributor.author","PIESKE, B"],["dc.date.accessioned","2021-12-08T12:27:26Z"],["dc.date.available","2021-12-08T12:27:26Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1016/S1567-4215(08)60144-7"],["dc.identifier.pii","S1567421508601447"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/95351"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-476"],["dc.relation.issn","1567-4215"],["dc.title","Cardiac function during mild hypothermia in human-sized pigs: Increased inotropy at the cost of diastolic dysfunction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","093117"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Chaos: An Interdisciplinary Journal of Nonlinear Science"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Lebert, Jan"],["dc.contributor.author","Christoph, Jan"],["dc.date.accessioned","2020-12-10T18:12:44Z"],["dc.date.available","2020-12-10T18:12:44Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1063/1.5101041"],["dc.identifier.eissn","1089-7682"],["dc.identifier.issn","1054-1500"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74477"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Synchronization-based reconstruction of electromechanical wave dynamics in elastic excitable media"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","123134"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Chaos: An Interdisciplinary Journal of Nonlinear Science"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Christoph, Jan"],["dc.contributor.author","Lebert, Jan"],["dc.date.accessioned","2021-04-14T08:26:02Z"],["dc.date.available","2021-04-14T08:26:02Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1063/5.0023751"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81809"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1089-7682"],["dc.relation.issn","1054-1500"],["dc.title","Inverse mechano-electrical reconstruction of cardiac excitation wave patterns from mechanical deformation using deep learning"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","667"],["dc.bibliographiccitation.issue","7698"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","672"],["dc.bibliographiccitation.volume","555"],["dc.contributor.author","Christoph, J."],["dc.contributor.author","Chebbok, M."],["dc.contributor.author","Richter, C."],["dc.contributor.author","Schröder-Schetelig, J."],["dc.contributor.author","Bittihn, P."],["dc.contributor.author","Stein, S."],["dc.contributor.author","Uzelac, I."],["dc.contributor.author","Fenton, F. H."],["dc.contributor.author","Hasenfuß, G."],["dc.contributor.author","Gilmour Jr., R. F."],["dc.contributor.author","Luther, S."],["dc.date.accessioned","2018-04-23T11:47:56Z"],["dc.date.available","2018-04-23T11:47:56Z"],["dc.date.issued","2018"],["dc.description.abstract","The self-organized dynamics of vortex-like rotating waves, which are also known as scroll waves, are the basis of the formation of complex spatiotemporal patterns in many excitable chemical and biological systems1,2,3,4. In the heart, filament-like phase singularities5,6 that are associated with three-dimensional scroll waves7 are considered to be the organizing centres of life-threatening cardiac arrhythmias7,8,9,10,11,12,13. The mechanisms that underlie the onset, maintenance and control14,15,16 of electromechanical turbulence in the heart are inherently three-dimensional phenomena. However, it has not previously been possible to visualize the three-dimensional spatiotemporal dynamics of scroll waves inside cardiac tissues. Here we show that three-dimensional mechanical scroll waves and filament-like phase singularities can be observed deep inside the contracting heart wall using high-resolution four-dimensional ultrasound-based strain imaging. We found that mechanical phase singularities co-exist with electrical phase singularities during cardiac fibrillation. We investigated the dynamics of electrical and mechanical phase singularities by simultaneously measuring the membrane potential, intracellular calcium concentration and mechanical contractions of the heart. We show that cardiac fibrillation can be characterized using the three-dimensional spatiotemporal dynamics of mechanical phase singularities, which arise inside the fibrillating contracting ventricular wall. We demonstrate that electrical and mechanical phase singularities show complex interactions and we characterize their dynamics in terms of trajectories, topological charge and lifetime. We anticipate that our findings will provide novel perspectives for non-invasive diagnostic imaging and therapeutic applications."],["dc.identifier.doi","10.1038/nature26001"],["dc.identifier.gro","3142312"],["dc.identifier.pmid","29466325"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/13445"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/208"],["dc.language.iso","en"],["dc.notes.intern","lifescience updates Crossref Import"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C03: Erholung nach Herzinsuffizienz: Analyse der transmuralen mechano-elektrischen Funktionsstörung"],["dc.relation","SFB 1002 | D01: Erholung aus der Herzinsuffizienz – Einfluss von Fibrose und Transkriptionssignatur"],["dc.relation.issn","0028-0836"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG Luther (Biomedical Physics)"],["dc.title","Electromechanical vortex filaments during cardiac fibrillation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]