Now showing 1 - 10 of 20
  • 2015Journal Article
    [["dc.bibliographiccitation.firstpage","278"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","CRYOLETTERS"],["dc.bibliographiccitation.lastpage","284"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Abdussamad, A. M."],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T09:54:52Z"],["dc.date.available","2018-11-07T09:54:52Z"],["dc.date.issued","2015"],["dc.description.abstract","Two experiments were conducted. The purpose of Experiment 1 was to investigate whether viability of bovine semen stored in liquid nitrogen (-196 degrees C) will be adversely affected by temporary exposure to dry ice (-79 degrees C). It was convincingly shown that post thaw-motility was not affected, regardless whether semen was thawed immediately or after being returned to liquid nitrogen. Shipping or temporary storage on dry ice, thus, is a viable option. In Experiment 2, refreezing of frozen-thawed semen was attempted. The proportion of motile spermatozoa was reduced by a factor of ten to between 6.0% and 7.4%, regardless whether thawing occurred directly after removal from liquid nitrogen or after an interim period on dry ice. When semen was refrozen on dry ice before being returned to liquid nitrogen, motility rates were significantly improved (13.0% to 17.0%, P<0.05). In both experiments sperm cells that remained motile displayed vigorous forward movement and normal morphological appearance."],["dc.identifier.isi","000361262800007"],["dc.identifier.pmid","26576003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36629"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cryo Letters"],["dc.relation.issn","1742-0644"],["dc.relation.issn","0143-2044"],["dc.title","TEMPORARY STORAGE OF BOVINE SEMEN CRYOPRESERVED IN LIQUID NITROGEN ON DRY ICE AND REFREEZING OF FROZEN-THAWED SEMEN"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2012Journal Article
    [["dc.bibliographiccitation.firstpage","77"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","REPRODUCTION"],["dc.bibliographiccitation.lastpage","81"],["dc.bibliographiccitation.volume","144"],["dc.contributor.author","Saleh, Malek"],["dc.contributor.author","Shahin, M."],["dc.contributor.author","Wuttke, Wolfgang"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T09:08:47Z"],["dc.date.available","2018-11-07T09:08:47Z"],["dc.date.issued","2012"],["dc.description.abstract","The present investigation addresses the pharmacokinetics of human chorionic gonadotropin (hCG), intramuscularly (i.m.) administered to goats. Nine pluriparous does of the Boer goat breed, 2-6 years of age and weighing 45-60 kg, were administered 500 IU hCG (2 ml Chorulon) deep into the thigh musculature 18 h after superovulatory FSH treatment. Blood samples were drawn from the jugular vein at 2 h intervals for the first 24 h, at 6 h intervals until 42 h, and at 12 h intervals until 114 h after administration. After centrifugation, plasma hCG concentrations were determined by electrochemiluminescence immunoassay. Pharmacokinetical parameters were as follows: lag time, 0.4 (S. E. M. 0.1) h; absorption rate constant, 0.34 (S. E. M. 0.002) h; absorption half-life, 2.7 (S. E. M. 0.5) h; elimination rate constant, 0.02 (S. E. M. 0.002) h; biological half-life, 39.4 (S. E. M. 5.1) h; and apparent volume of distribution, 16.9 (S. E. M. 4.3) I. The plasma hCG profile was characterized by an absorption phase of 11.6 (S. E. M. 1.8) h and an elimination phase of 70.0 (S. E. M. 9.8) h, with considerable individual variation in bioavailability and pharmacokinetical parameters. Biological half-life was negatively correlated (P<0.05) with peak concentration (r=-0.76), absorption rate constant (r=-0.78), and elimination rate constant (r=-0.87). The results indicate that after rapid absorption, hCG remains in the circulation for an extended period. This has to be taken into account when assessing the stimulatory response to hCG treatment on an ovarian level. Reproduction (2012) 144 77-81"],["dc.identifier.doi","10.1530/REP-12-0093"],["dc.identifier.isi","000305822000007"],["dc.identifier.pmid","22573828"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26107"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Bioscientifica Ltd"],["dc.relation.issn","1470-1626"],["dc.title","Pharmacokinetics of human chorionic gonadotropin after i.m. administration in goats (Capra hircus)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2013Journal Article
    [["dc.bibliographiccitation.firstpage","466"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","CRYOLETTERS"],["dc.bibliographiccitation.lastpage","470"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Al Yacoub, Azzam"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T09:20:02Z"],["dc.date.available","2018-11-07T09:20:02Z"],["dc.date.issued","2013"],["dc.description.abstract","The study addresses the relevance of sucrose with OPS vitrification of murine blastocysts. In a 3x3 factorial experiment, blastocysts were subjected to vitrification solution (20% Me(2)S0 and 20% EG) containing 0.0, 0.4 or 0.8M sucrose and warming solution (HEPES buffered TCM199) containing 0.00, 0.25 or 0.50M sucrose. After 48h of in vitro culture, with 0.4 M sucrose in vitrification solution, 84-87% of embryos had reached the expanded blastocyst stage, as compared to 76-82% with 0.0M and 40-54% (P<0.01) with 0.8M sucrose. Hatching rates confirmed the tendency. The sucrose content of the warming solution had no significant effect on expansion or hatching rates (P>0.05). It may be concluded that, whereas, vitrification solution should contain a moderate concentration of sucrose, in dilution medium sucrose is dispensable. This implies that embryos may be transferred directly after warming, which, if applicable to farm animals, would greatly facilitate vitrification in practice."],["dc.description.sponsorship","German Academic Exchange Service (DAAD) fellowship"],["dc.identifier.isi","000337718600002"],["dc.identifier.pmid","24448766"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28781"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cryo Letters"],["dc.relation.issn","1742-0644"],["dc.relation.issn","0143-2044"],["dc.title","IS SUCROSE REQUIRED IN OPEN PULLED STRAW (OPS) VITRIFICATION OF MOUSE EMBRYOS?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2011Conference Abstract
    [["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Reproduction Fertility and Development"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","El-Gayar, M."],["dc.contributor.author","Reischl, Judith B."],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T09:00:24Z"],["dc.date.available","2018-11-07T09:00:24Z"],["dc.date.issued","2011"],["dc.format.extent","141"],["dc.identifier.isi","285008200084"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24150"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Csiro Publishing"],["dc.publisher.place","Collingwood"],["dc.relation.issn","1031-3613"],["dc.title","WILL DEHYDRATION BY EXPOSURE TO SUCROSE IMPROVE POST-VITRIFICATION SURVIVAL OF MURINE EMBRYOS VITRIFIED BY THE OPEN PULLED STRAW METHOD?"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2017Journal Article
    [["dc.bibliographiccitation.firstpage","26"],["dc.bibliographiccitation.journal","Small Ruminant Research"],["dc.bibliographiccitation.lastpage","31"],["dc.bibliographiccitation.volume","151"],["dc.contributor.author","Camacho, Miguel"],["dc.contributor.author","Stiehl, Jennifer"],["dc.contributor.author","Garza, Denisse"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2020-12-10T15:21:18Z"],["dc.date.available","2020-12-10T15:21:18Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.smallrumres.2017.04.008"],["dc.identifier.issn","0921-4488"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72980"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Seasonality of Boer goats in northern Europe and induction of estrus out of season"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
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  • 2012Conference Abstract
    [["dc.bibliographiccitation.journal","Reproduction in Domestic Animals"],["dc.bibliographiccitation.volume","47"],["dc.contributor.author","Marthold, D."],["dc.contributor.author","Detterer, J."],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T09:13:11Z"],["dc.date.available","2018-11-07T09:13:11Z"],["dc.date.issued","2012"],["dc.format.extent","36"],["dc.identifier.isi","000300500500111"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/27116"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.conference","45th Annual Conference of Physiology and Pathology of Reproduction/37th Joint Conference on Veterinary and Human Reproduction Medicine/1st Joint German-Polish Conference on Reproductive Medicine"],["dc.relation.eventlocation","Berlin, GERMANY"],["dc.relation.issn","0936-6768"],["dc.title","Fixed-time insemination following an Ovsynch regime in dairy cows - a field trial"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2011Conference Abstract
    [["dc.bibliographiccitation.firstpage","141"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Reproduction Fertility and Development"],["dc.bibliographiccitation.lastpage","142"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","El-Gayar, M."],["dc.contributor.author","Al Yacoub, Azzam"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T09:00:24Z"],["dc.date.available","2018-11-07T09:00:24Z"],["dc.date.issued","2011"],["dc.identifier.isi","000285008200085"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24149"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Csiro Publishing"],["dc.publisher.place","Collingwood"],["dc.relation.issn","1031-3613"],["dc.title","SUPERIOR PREGNANCY RATES WITH THE TRANSFER OF OPEN PULLED STRAW-VITRIFIED V. CONVENTIONALLY CRYOPRESERVED EMBRYOS IN GOATS"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2008Conference Abstract
    [["dc.bibliographiccitation.journal","Reproduction in Domestic Animals"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Schmitz, W."],["dc.contributor.author","Driancourt, M. A."],["dc.contributor.author","Hoppe, Sebastian"],["dc.contributor.author","Friedrich, M."],["dc.contributor.author","Erhardt, Georg"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T11:13:49Z"],["dc.date.available","2018-11-07T11:13:49Z"],["dc.date.issued","2008"],["dc.format.extent","182"],["dc.identifier.isi","000260529300536"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53985"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.publisher.place","Malden"],["dc.relation.conference","16th International Congress on Animal Reproduction"],["dc.relation.eventlocation","Budapest, HUNGARY"],["dc.relation.issn","0936-6768"],["dc.title","A modified Cosynch protocol for timed artificial insemination in beef cattle"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2010Journal Article
    [["dc.bibliographiccitation.firstpage","1018"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Theriogenology"],["dc.bibliographiccitation.lastpage","1023"],["dc.bibliographiccitation.volume","73"],["dc.contributor.author","Al Yacoub, Azzam"],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.date.accessioned","2018-11-07T08:43:54Z"],["dc.date.available","2018-11-07T08:43:54Z"],["dc.date.issued","2010"],["dc.description.abstract","This investigation addresses the question whether it is possible to apply the open pulled straw (OPS) vitrification method, found to be effective for cryopreserving caprine (Capra aegagrus horns) blastocysts, to other embryonal stages Morulae. blastocysts and hatched blastocysts were cryopreserved by way of OPS vitrification and blastocysts and hatched blastocysts by conventional freezing Morulae were not included with conventional freezing because in our experience the survival tale is vets' low To assess the viability of the cryopreserved embryos, they were transferred to synchronized does, in most cases, two embryos per doe After OPS vitrification, of rune does receiving morulae, not a single one became pregnant, of 11 does receiving blastocysts. nine (82%) became pregnant (all of which kidded and gave birth to. on average. 1 8 kids), and of nine does receiving hatched blastocysts. three (33%) became pregnant (two of which [22%] kidded, giving birth to a single kid each) After conventional freezing. of 10 does receiving blastocysts, five became pregnant (four of which [40%] carried to term and gave birth to a pair of twins each), and of nine does receiving hatched blastocysts. three (33%) became pregnant (and gave birth to a single kid each) Embryo survival (kids born/embryos transferred) after vitrification for morulae, blastocysts, and hatched blastocysts was 0.70% (16 of 23), and 13% (2 of 16), respectively, and after conventional freezing for blastocysts and hatched blastocysts was 42% (8 of 19) and 19% (3 of 16), respectively The difference in pregnancy and kidding rate between vitrified and conventionally frozen blastocysts was significant. and so was the difference in pregnancy rate between hatched and nonhatched blastocysts, regardless whether OPS-vitrified or conventionally frozen The results of the current study indicate that OPS vitrification is a very effective means of cryopreserving caprine blastocysts Unfortunately, the superiority of OPS vitrification over conventional freezing does not apply to caprine morulae and hatched blastocysts (C) 2010 Elsevier Inc All rights reserved"],["dc.identifier.doi","10.1016/j.theriogenology.2009.11.028"],["dc.identifier.isi","000276801400003"],["dc.identifier.pmid","20102780"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20082"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","0093-691X"],["dc.title","Open pulled straw vitrification of goat embryos at various stages of development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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  • 2013Journal Article
    [["dc.bibliographiccitation.firstpage","6520"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Journal of Dairy Science"],["dc.bibliographiccitation.lastpage","6524"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Stahmann, F."],["dc.contributor.author","Gauly, Matthias"],["dc.contributor.author","Holtz, Wolfgang"],["dc.contributor.author","von Borstel, Uta Konig"],["dc.date.accessioned","2018-11-07T09:19:20Z"],["dc.date.available","2018-11-07T09:19:20Z"],["dc.date.issued","2013"],["dc.description.abstract","Early pregnancy detection is a measure of considerable economic relevance for dairy cattle breeders, and analysis of pregnancy-associated glycoprotein (PAG) values in blood is one of the methods implemented in practice. Starting from d 30 postconception, cows are considered to be pregnant at PAG levels of 2.0 ng of PAG/mL of blood and higher. However, little is known about preanalytic sources of errors that might affect PAG values. Based on blood samples from 65 dairy cows, the present study showed that freezing of samples, such as may be the case during shipping in wintertime, will lower PAG values considerably. Therefore, a Bland-Altman analysis was used to derive a correction factor. Overall, the mean differences (+/- standard deviation) between frozen and respective fresh samples was -5.5 +/- 7.4 ng of PAG/mL of blood and 0.9 +/- 6.1 ng of PAG/mL of serum. However, the Bland-Altman plot revealed a concentration-dependent effect of freezing on PAG values with higher variability and larger declines at higher FAG levels. Therefore, to minimize chances of false-negative results, different correction factors are suggested for different levels of FAG (e.g., based on the upper bound of the 95% confidence interval 0.67 for PAG levels between 2.0 and 3.9 ng of PAG/mL and 0.25 for PAG levels between 4.0 and 7.9 ng of PAG/mL). With these concentration-dependent correction factors, implementation into practice will be possible. The accuracy is adequate because no quantitative information but qualitative results (pregnant vs. nonpregnant) are required. However, due to larger chances of false-negative results, the application of the correction factor should only be a last resort if temperature exposure of a sample is unknown."],["dc.identifier.doi","10.3168/jds.2013-6734"],["dc.identifier.isi","000324657700036"],["dc.identifier.pmid","23932137"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28610"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Inc"],["dc.relation.issn","1525-3198"],["dc.relation.issn","0022-0302"],["dc.title","Short communication: A note on the correction for the effect of freezing on the outcome of pregnancy-associated glycoprotein measurement in blood and serum of cows"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
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