Noll, Angela

[["dc.bibliographiccitation.artnumber","e55232"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Bennstein, Sabrina Bianca"],["dc.contributor.author","Weinhold, Sandra"],["dc.contributor.author","Manser, Angela Riccarda"],["dc.contributor.author","Scherenschlich, Nadine"],["dc.contributor.author","Noll, Angela"],["dc.contributor.author","Raba, Katharina"],["dc.contributor.author","Kögler, Gesine"],["dc.contributor.author","Walter, Lutz"],["dc.contributor.author","Uhrberg, Markus"],["dc.date.accessioned","2022-10-06T13:26:56Z"],["dc.date.available","2022-10-06T13:26:56Z"],["dc.date.issued","2020"],["dc.description.abstract","Despite their identification several years ago, molecular identity and developmental relation between human ILC1 and NK cells, comprising group 1 ILCs, is still elusive. To unravel their connection, thorough transcriptional, epigenetic, and functional characterization was performed from umbilical cord blood (CB). Unexpectedly, ILC1-like cells lacked Tbet expression and failed to produce IFNγ. Moreover, in contrast to previously described ILC1 subsets they could be efficiently differentiated into NK cells. These were characterized by highly diversified KIR repertoires including late stage NKG2A-KIR+ effector cells that are commonly not generated from previously known NK cell progenitor sources. This property was dependent on stroma cell-derived Notch ligands. The frequency of the novel ILC1-like NK cell progenitor (NKP) significantly declined in CB from early to late gestational age. The study supports a model in which circulating fetal ILC1-like NKPs travel to secondary lymphoid tissues to initiate the formation of diversified NK cell repertoires after birth."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.7554/eLife.55232"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115204"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2050-084X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","867"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Stem Cells Translational Medicine"],["dc.bibliographiccitation.lastpage","882"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Bennstein, Sabrina Bianca"],["dc.contributor.author","Scherenschlich, Nadine"],["dc.contributor.author","Weinhold, Sandra"],["dc.contributor.author","Manser, Angela Riccarda"],["dc.contributor.author","Noll, Angela"],["dc.contributor.author","Raba, Katharina"],["dc.contributor.author","Kögler, Gesine"],["dc.contributor.author","Walter, Lutz"],["dc.contributor.author","Uhrberg, Markus"],["dc.date.accessioned","2022-10-06T13:25:52Z"],["dc.date.available","2022-10-06T13:25:52Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract\n Innate lymphoid cells (ILCs), comprising ILC1, 2, and 3 subpopulations, play unique roles in maintaining microbiome homeostasis, mucosal tissue integrity, and control of inflammation. So far, their characterization is dominantly based on tissue-resident ILCs, whereas little information is available on circulating ILCs, in particular in newborns. In order to get a deeper understanding of neonatal innate immunity, we analyzed the transcriptomes and effector functions of cord blood (CB) ILCs. By RNAseq analysis, all ILC subsets could be clearly distinguished from each other. CB-derived ILCs were generally closer related to neonatal T than natural killer (NK) cells and several factors shared by all three ILC subsets such as CD28, CCR4, and SLAMF1 are commonly expressed by T cells but lacking in NK cells. Notably, CB ILCs exhibited a unique signature of DNA binding inhibitor (ID) transcription factors (TF) with high ID3 and low ID2 expression distinct from PB- or tonsil-derived ILCs. In vitro stimulation of sorted CB ILCs revealed distinct differences to tissue-resident ILCs in that ILC1-like and ILC3-like cells were nonresponsive to specific cytokine stimulation, indicating functional immaturity. However, CB ILC3-like cells expressed toll-like receptors TLR1 and TLR2 and upon stimulation with the TLR2:1 ligand Pam3CSK4, responded with significantly increased proliferation and cytokine secretion. Together, our data provide novel insights into neonatal ILC biology with a unique TF signature of CB ILCs possibly indicating a common developmental pathway and furthermore a role of CB ILC3-like cells in innate host defense."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1002/sctm.20-0300"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114934"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","2157-6580"],["dc.relation.issn","2157-6564"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","http://creativecommons.org/licenses/by-nc-nd/4.0/"],["dc.title","Transcriptional and functional characterization of neonatal circulating Innate Lymphoid Cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]