Kusch, Harald

[["dc.bibliographiccitation.firstpage","245"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Archives of Microbiology"],["dc.bibliographiccitation.lastpage","267"],["dc.bibliographiccitation.volume","197"],["dc.contributor.author","Neumann, Yvonne"],["dc.contributor.author","Ohlsen, Knut"],["dc.contributor.author","Donat, Stefanie"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Cartron, Michael"],["dc.contributor.author","Hurd, Alexander"],["dc.contributor.author","Foster, Simon J."],["dc.date.accessioned","2018-11-07T10:00:42Z"],["dc.date.available","2018-11-07T10:00:42Z"],["dc.date.issued","2015"],["dc.description.abstract","Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS."],["dc.description.sponsorship","European Union; MRC [78981]; DFG [TRR34]"],["dc.identifier.doi","10.1007/s00203-014-1048-1"],["dc.identifier.isi","000349391400011"],["dc.identifier.pmid","25325933"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11155"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37864"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-072X"],["dc.relation.issn","0302-8933"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The effect of skin fatty acids on Staphylococcus aureus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e70669"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PloS one"],["dc.bibliographiccitation.lastpage","e70669"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Fuchs, Stephan"],["dc.contributor.author","Zühlke, Daniela"],["dc.contributor.author","Pané-Farré, Jan"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Wolf, Carmen"],["dc.contributor.author","Reiß, Swantje"],["dc.contributor.author","Binh, Le Thi Nguyen"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Riedel, Katharina"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Engelmann, Susanne"],["dc.date.accessioned","2019-07-09T11:40:11Z"],["dc.date.available","2019-07-09T11:40:11Z"],["dc.date.issued","2013"],["dc.description.abstract","Gel-based proteomics is a powerful approach to study the physiology of Staphylococcus aureus under various growth restricting conditions. We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins. 4,692 time dependent protein synthesis profiles were generated by exposing S. aureus to nine infection-related stress and starvation stimuli (H2O2, diamide, paraquat, NO, fermentation, nitrate respiration, heat shock, puromycin, mupirocin). These expression profiles are stored in an online resource called Aureolib (http://www.aureolib.de). Moreover, information on target genes of 75 regulators and regulatory elements were included in the database. Cross-comparisons of this extensive data collection of protein synthesis profiles using the tools implemented in Aureolib lead to the identification of stress and starvation specific marker proteins. Altogether, 226 protein synthesis profiles showed induction ratios of 2.5-fold or higher under at least one of the tested conditions with 157 protein synthesis profiles specifically induced in response to a single stimulus. The respective proteins might serve as marker proteins for the corresponding stimulus. By contrast, proteins whose synthesis was increased or repressed in response to more than four stimuli are rather exceptional. The only protein that was induced by six stimuli is the universal stress protein SACOL1759. Most strikingly, cluster analyses of synthesis profiles of proteins differentially synthesized under at least one condition revealed only in rare cases a grouping that correlated with known regulon structures. The most prominent examples are the GapR, Rex, and CtsR regulon. In contrast, protein synthesis profiles of proteins belonging to the CodY and σ(B) regulon are widely distributed. In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction."],["dc.identifier.doi","10.1371/journal.pone.0070669"],["dc.identifier.fs","597423"],["dc.identifier.pmid","23967085"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10724"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58109"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Adaptation, Biological"],["dc.subject.mesh","Bacterial Proteins"],["dc.subject.mesh","Cluster Analysis"],["dc.subject.mesh","Computational Biology"],["dc.subject.mesh","Databases, Genetic"],["dc.subject.mesh","Gene Expression Regulation, Bacterial"],["dc.subject.mesh","Hydrogen Peroxide"],["dc.subject.mesh","Proteome"],["dc.subject.mesh","Proteomics"],["dc.subject.mesh","Signal Transduction"],["dc.subject.mesh","Staphylococcus aureus"],["dc.subject.mesh","Stress, Physiological"],["dc.subject.mesh","User-Computer Interface"],["dc.title","Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]