Kusch, Harald

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[["dc.bibliographiccitation.firstpage","291"],["dc.bibliographiccitation.issue","3/4"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","318"],["dc.bibliographiccitation.volume","298"],["dc.contributor.author","Bode, Rüdiger"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Morschhäuser, Joachim"],["dc.creator.author","Hecker M"],["dc.creator.author","Bode R"],["dc.creator.author","Kusch H"],["dc.creator.author","Engelmann S"],["dc.creator.author","Albrecht D"],["dc.creator.author","Morschhäuser J"],["dc.date.accessioned","2019-10-09T09:38:18Z"],["dc.date.available","2019-10-09T09:38:18Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1016/j.ijmm.2007.03.020"],["dc.identifier.pmid","17588813"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62478"],["dc.language.iso","en"],["dc.title","A proteomic view of Candida albicans yeast cell metabolism in exponential and stationary growth phases"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3518"],["dc.bibliographiccitation.issue","17"],["dc.bibliographiccitation.journal","Proteomics"],["dc.bibliographiccitation.lastpage","3530"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Winter, Theresa"],["dc.contributor.author","Winter, Jörn"],["dc.contributor.author","Polak, Martin"],["dc.contributor.author","Kusch, Kathrin"],["dc.contributor.author","Mäder, Ulrike"],["dc.contributor.author","Sietmann, Rabea"],["dc.contributor.author","Ehlbeck, Jörg"],["dc.contributor.author","van Hijum, Sacha"],["dc.contributor.author","Weltmann, Klaus-Dieter"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Kusch, Harald"],["dc.date.accessioned","2019-08-06T11:02:44Z"],["dc.date.available","2019-08-06T11:02:44Z"],["dc.date.issued","2011"],["dc.description.abstract","Plasma medicine and also decontamination of bacteria with physical plasmas is a promising new field of life science with huge interest especially for medical applications. Despite numerous successful applications of low temperature gas plasmas in medicine and decontamination, the fundamental nature of the interactions between plasma and microorganisms is to a large extent unknown. A detailed knowledge of these interactions is essential for the development of new as well as for the enhancement of established plasma-treatment procedures. In the present work we introduce for the first time a growth chamber system suitable for low temperature gas plasma treatment of bacteria in liquid medium. We have coupled the use of this apparatus to a combined proteomic and transcriptomic analyses to investigate the specific stress response of Bacillus subtilis 168 cells to treatment with argon plasma. The treatment with three different discharge voltages revealed not only effects on growth, but also clear evidence of cellular stress responses. B. subtilis suffered severe cell wall stress, which was made visible also by electron microscopy, DNA damages and oxidative stress as a result of exposure to plasma. These biological findings were supported by the detection of reactive plasma species by OES measurements."],["dc.identifier.doi","10.1002/pmic.201000637"],["dc.identifier.pmid","21751354"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62304"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1615-9861"],["dc.relation.issn","1615-9853"],["dc.title","Characterization of the global impact of low temperature gas plasma on vegetative microorganisms"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","554"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Molecular Genetics and Genomics"],["dc.bibliographiccitation.lastpage","565"],["dc.bibliographiccitation.volume","271"],["dc.contributor.author","Schwanfelder, S"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Biswas, K"],["dc.contributor.author","Rogers, PD"],["dc.contributor.author","Morschhäuser, Joachim"],["dc.contributor.author","Hecker, Michael"],["dc.creator.author","Schwanfelder S"],["dc.creator.author","Hecker M"],["dc.creator.author","Engelmann S"],["dc.creator.author","Biswas K"],["dc.creator.author","Kusch H"],["dc.creator.author","Rogers PD"],["dc.creator.author","Morschhäuser J"],["dc.date.accessioned","2019-10-09T09:04:41Z"],["dc.date.available","2019-10-09T09:04:41Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1007/s00438-004-0984-x"],["dc.identifier.pmid","15114480"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62474"],["dc.language.iso","en"],["dc.title","A proteomic approach to understanding the development of multidrug-resistant Candida albicans strains"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Leonard, Miriam"],["dc.contributor.author","Kühn, Anika"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Maurus, Isabel"],["dc.contributor.author","Nagel, Alexandra"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Becher, Dörte"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2021-04-14T08:23:50Z"],["dc.date.available","2021-04-14T08:23:50Z"],["dc.date.issued","2020"],["dc.description.abstract","Verticillia cause a vascular wilt disease affecting a broad range of economically valuable crops. The fungus enters its host plants through the roots and colonizes the vascular system. It requires extracellular proteins for a successful plant colonization. The exoproteomes of the allodiploid Verticillium longisporum upon cultivation in different media or xylem sap extracted from its host plant Brassica napus were compared. Secreted fungal proteins were identified by label free liquid chromatography-tandem mass spectrometry screening. V. longisporum induced two main secretion patterns. One response pattern was elicited in various non-plant related environments. The second pattern includes the exoprotein responses to the plant-related media, pectin-rich simulated xylem medium and pure xylem sap, which exhibited similar but additional distinct features. These exoproteomes include a shared core set of 221 secreted and similarly enriched fungal proteins. The pectin-rich medium significantly induced the secretion of 143 proteins including a number of pectin degrading enzymes, whereas xylem sap triggered a smaller but unique fungal exoproteome pattern with 32 enriched proteins. The latter pattern included proteins with domains of known pathogenicity factors, metallopeptidases and carbohydrate-active enzymes. The most abundant proteins of these different groups are the necrosis and ethylene inducing-like proteins Nlp2 and Nlp3, the cerato-platanin proteins Cp1 and Cp2, the metallopeptidases Mep1 and Mep2 and the carbohydrate-active enzymes Gla1, Amy1 and Cbd1. Their pathogenicity contribution was analyzed in the haploid parental strain V. dahliae. Deletion of the majority of the corresponding genes caused no phenotypic changes during ex planta growth or invasion and colonization of tomato plants. However, we discovered that the MEP1, NLP2, and NLP3 deletion strains were compromised in plant infections. Overall, our exoproteome approach revealed that the fungus induces specific secretion responses in different environments. The fungus has a general response to non-plant related media whereas it is able to fine-tune its exoproteome in the presence of plant material. Importantly, the xylem sap-specific exoproteome pinpointed Nlp2 and Nlp3 as single effectors required for successful V. dahliae colonization."],["dc.identifier.doi","10.3389/fmicb.2020.01876"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17508"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81068"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-302X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Verticillium longisporum Elicits Media-Dependent Secretome Responses With Capacity to Distinguish Between Plant-Related Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3914"],["dc.bibliographiccitation.issue","19"],["dc.bibliographiccitation.journal","Proteomics"],["dc.bibliographiccitation.lastpage","3927"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Kolata, Julia"],["dc.contributor.author","Bode, Lonneke G. M."],["dc.contributor.author","Holtfreter, Silva"],["dc.contributor.author","Steil, Leif"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Holtfreter, Birte"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","van Belkum, Alex"],["dc.contributor.author","Volker, Uwe"],["dc.contributor.author","Bröker, Barbara M."],["dc.date.accessioned","2019-08-06T10:50:27Z"],["dc.date.available","2019-08-06T10:50:27Z"],["dc.date.issued","2011"],["dc.description.abstract","Staphylococcus aureus is both a prominent cause of nosocomial infections with significant morbidity and mortality and a commensal with nasal carriage in around 30% of the population. The rapid spread of multi-resistant strains necessitates novel therapeutic strategies, a challenging task because the species S. aureus and the host response against it are highly variable. In a prospective study among 2023 surgical and non-surgical patients, 12 patients developed S. aureus bacteremia. They were analysed in detail using a personalized approach. For each patient, the extracellular proteins of the infecting S. aureus strain were identified and the developing antibody response was assessed on 2-D immunoblots. S. aureus carriers showed clear evidence of strain-specific pre-immunization. In all immune-competent bacteremia patients, antibody binding increased strongly, in most cases already at diagnosis. In endogenous infections, the pattern of antibody binding was similar to the pre-infection pattern. In exogenous infections, in contrast, the pre-infection pattern was radically altered with the acquisition of new specificities. These were characteristic for individual patients. Nevertheless, a common signature of 11 conserved S. aureus proteins, recognized in at least half of the bacteremic patients, was identified. All patients mounted a dynamic antibody response to a subset of these proteins."],["dc.identifier.doi","10.1002/pmic.201000760"],["dc.identifier.pmid","21805632"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62303"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1615-9861"],["dc.relation.issn","1615-9853"],["dc.title","Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non-carriers"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1607"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Clinical and Vaccine Immunology"],["dc.bibliographiccitation.lastpage","1614"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Nguyen, TT"],["dc.contributor.author","Steil, Leif"],["dc.contributor.author","Bröker, Barbara M."],["dc.contributor.author","van Belkum, Alexander"],["dc.contributor.author","Holtfreter, Silva"],["dc.contributor.author","Wertheim, H"],["dc.contributor.author","Truong, QP"],["dc.contributor.author","Engelmann, Susanne"],["dc.creator.author","van Belkum A"],["dc.creator.author","Engelmann S"],["dc.creator.author","Nguyen TT"],["dc.creator.author","Wertheim H"],["dc.creator.author","Steil L"],["dc.creator.author","Truong QP"],["dc.creator.author","Bröker BM"],["dc.creator.author","Holtfreter S"],["dc.creator.author","Völker U"],["dc.creator.author","Hecker M"],["dc.creator.author","Kusch H"],["dc.date.accessioned","2019-10-09T09:43:16Z"],["dc.date.available","2019-10-09T09:43:16Z"],["dc.date.issued","2009"],["dc.identifier.doi","10.1128/CVI.00263-09"],["dc.identifier.pmid","19759252"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62480"],["dc.language.iso","en"],["dc.title","Human immune proteome in experimental colonization with Staphylococcus aureus"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Leonard, Miriam"],["dc.contributor.author","Kühn, Anika"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Maurus, Isabel"],["dc.contributor.author","Nagel, Alexandra"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Becher, Dörte"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2020-03-30T08:55:04Z"],["dc.date.available","2020-03-30T08:55:04Z"],["dc.date.issued","2020-02-12"],["dc.description.abstract","Verticillia cause a vascular wilt disease affecting a broad range of economically valuable crops. The fungus enters its host plants through the roots and colonizes the vascular system. It requires extracellular proteins for a successful plant colonization. The exoproteome of the allodiploid Verticillium longisporum was analyzed upon cultivation in different media. Secreted fungal proteins were identified by label free LC-MS/MS screening. V. longisporum induced two main secretion patterns. One response pattern was elicited in various non-plant related environments. The second pattern includes the exoprotein responses to the plant-related media, pectin-rich simulated xylem medium and pure xylem sap, which exhibited similar but additional distinct features. These exoproteomes include a shared core set of 223 secreted and similarly enriched fungal proteins. The pectin-rich medium significantly induced the secretion of 144 proteins including a number of pectin degrading enzymes, whereas xylem sap triggered a smaller but unique fungal exoproteome pattern with 32 enriched proteins. The latter pattern included proteins with domains of known effectors, metallopeptidases and carbohydrate-active enzymes. The most abundant and uniquely enriched proteins of these different groups are the necrosis and ethylene inducing-like proteins Nlp2 and Nlp3, the cerato-platanin proteins Cp1 and Cp2, the metallopeptidases Mep1 and Mep2 and the CAZys Gla1, Amy1 and Cbd1. Deletion of the majority of the corresponding genes caused no phenotypic changes during ex planta growth or invasion and colonization of tomato plants. However, we discovered that the NLP2 and NLP3 deletion strains were compromised in plant infections. Overall, our exoproteome approach revealed that the fungus induces specific secretion responses in different environments. The fungus has a general response to non-plant related media whereas it is able to fine-tune its exoproteome in the presence of plant material. Importantly, the xylem sap-specific exoproteome pinpointed Nlp2 and Nlp3 as single effectors required for successful V. dahliae colonization."],["dc.format.extent","59"],["dc.identifier.doi","10.1101/2020.02.11.943803"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63413"],["dc.language.iso","en"],["dc.title","V. longisporum elicits media-dependent secretome responses with a further capacity to distinguish between plant-related environments"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1825"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Infection and Immunity"],["dc.bibliographiccitation.lastpage","1836"],["dc.bibliographiccitation.volume","76"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","Uhlin, BE"],["dc.contributor.author","Hippenstiel, S"],["dc.contributor.author","Galka, Frank"],["dc.contributor.author","Wai, SN"],["dc.contributor.author","Schmeck, B"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Steinert, M"],["dc.contributor.author","Hecker, Michael"],["dc.creator.author","Kusch H"],["dc.creator.author","Hippenstiel S"],["dc.creator.author","Uhlin BE"],["dc.creator.author","Galka F"],["dc.creator.author","Engelmann S"],["dc.creator.author","Schmeck B"],["dc.creator.author","Wai SN"],["dc.creator.author","Hecker M"],["dc.creator.author","Steinert M"],["dc.date.accessioned","2019-10-09T09:41:19Z"],["dc.date.available","2019-10-09T09:41:19Z"],["dc.date.issued","2008"],["dc.identifier.doi","10.1128/IAI.01396-07"],["dc.identifier.pmid","18250176"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62479"],["dc.language.iso","en"],["dc.title","Proteomic characterization of the whole secretome of Legionella pneumophila and functional analysis of outer membrane vesicles"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.lastpage","1"],["dc.contributor.author","Winter, Jörn"],["dc.contributor.author","Reuter, S. P."],["dc.contributor.author","Winter, Theresa"],["dc.contributor.author","Kusch, Kathrin"],["dc.contributor.author","Sietmann, Rabea"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Polak, M."],["dc.contributor.author","Ehlbeck, J."],["dc.contributor.author","Weltmann, K.-D."],["dc.date.accessioned","2019-08-06T11:15:58Z"],["dc.date.available","2019-08-06T11:15:58Z"],["dc.date.issued","2011"],["dc.description.abstract","Summary form only given. Plasma medicine and also inactivation of bacteria with physical plasmas is a promising new field of life science with huge interest especially for medical applications. Despite numerous successful applications of low temperature gas plasmas in medicine and decontamination, the fundamental nature of the interactions between plasma and microorganisms is to a large extent unknown. A detailed knowledge of these interactions is essential for the development of new as well as for the enhancement of established plasma treatment procedures.In the present work we introduce a growth chamber system suitable for physical plasma treatment of bacteria in liquid medium. The gram positive model organism Bacillus subtilis was treated with plasma, in order to investigate the specific stress response using a proteomic and transcriptomic approach. Two different gas admixtures as well as different discharge voltages were applied during the plasma treatment. We were able to connect reactive agents produced in the plasma with the specific cellular response. This response displayed a clear correlation in dependence of the used gas and the plasma power. B. subtilis faces e.g. different kinds of cell wall stress, depending on the used gas, which was made visible also by electron microscopy. Furthermore, the extent of DNA damages and oxidative stress differed significantly, again, depending on the used gas and plasma power. The biological findings could be supported by the diagnostic of reactive plasma species."],["dc.identifier.doi","10.1109/PLASMA.2011.5993209"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62305"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.publisher","IEEE"],["dc.relation.conference","International Conference on Plasma Science"],["dc.relation.eventend","2011-06-30"],["dc.relation.eventlocation","Chicago, IL, USA"],["dc.relation.eventstart","2011-06-26"],["dc.relation.isbn","978-1-61284-330-8"],["dc.relation.ispartof","2011 Abstracts IEEE International Conference on Plasma Science"],["dc.title","Global characterization of physical plasma impact on vegetative microorganisms"],["dc.type","conference_paper"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]