Kusch, Harald

[["dc.bibliographiccitation.journal","Molecular Plant Pathology"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Pöggeler, Stefanie"],["dc.contributor.author","Maurus, Isabel"],["dc.contributor.author","Schlüter, Rabea"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Bulla, Ingo"],["dc.contributor.author","Nowrousian, Minou"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","de Jonge, Ronnie"],["dc.contributor.author","Stahlhut, Gertrud"],["dc.contributor.author","Hoff, Katharina J."],["dc.contributor.author","Aßhauer, Kathrin P."],["dc.contributor.author","Thürmer, Andrea"],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Thomma, Bart P. H. J."],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Braus‐Stromeyer, Susanna A."],["dc.date.accessioned","2021-06-01T09:42:04Z"],["dc.date.available","2021-06-01T09:42:04Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize the plant host Brassica napus but differ in their ability to cause disease symptoms. These strains represent two V. longisporum lineages derived from different hybridization events of haploid parental Verticillium strains. Vl32 and Vl43 carry same‐sex mating‐type genes derived from both parental lineages. Vl32 and Vl43 similarly colonize and penetrate plant roots, but asymptomatic Vl32 proliferation in planta is lower than virulent Vl43. The highly conserved Vl43 and Vl32 genomes include less than 1% unique genes, and the karyotypes of 15 or 16 chromosomes display changed genetic synteny due to substantial genomic reshuffling. A 20 kb Vl43 lineage‐specific (LS) region apparently originating from the Verticillium dahliae‐related ancestor is specific for symptomatic Vl43 and encodes seven genes, including two putative transcription factors. Either partial or complete deletion of this LS region in Vl43 did not reduce virulence but led to induction of even more severe disease symptoms in rapeseed. This suggests that the LS insertion in the genome of symptomatic V. longisporum Vl43 mediates virulence‐reducing functions, limits damage on the host plant, and therefore tames Vl43 from being even more virulent."],["dc.description.abstract","A lineage‐specific region in the Verticillium longisporum Vl43 genome reduces fungal virulence of infected rapeseed host plants. image"],["dc.description.sponsorship","Bundesministerium für Bildung und Forschung: BioFung http://dx.doi.org/10.13039/501100002347"],["dc.description.sponsorship","Natural Sciences and Engineering Research Council of Canada: CREATE http://dx.doi.org/10.13039/501100000038"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1111/mpp.13071"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85132"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1364-3703"],["dc.relation.issn","1464-6722"],["dc.relation.orgunit","Abteilung Molekulare Mikrobiologie & Genetik"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","245"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Archives of Microbiology"],["dc.bibliographiccitation.lastpage","267"],["dc.bibliographiccitation.volume","197"],["dc.contributor.author","Neumann, Yvonne"],["dc.contributor.author","Ohlsen, Knut"],["dc.contributor.author","Donat, Stefanie"],["dc.contributor.author","Engelmann, Susanne"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Cartron, Michael"],["dc.contributor.author","Hurd, Alexander"],["dc.contributor.author","Foster, Simon J."],["dc.date.accessioned","2018-11-07T10:00:42Z"],["dc.date.available","2018-11-07T10:00:42Z"],["dc.date.issued","2015"],["dc.description.abstract","Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS."],["dc.description.sponsorship","European Union; MRC [78981]; DFG [TRR34]"],["dc.identifier.doi","10.1007/s00203-014-1048-1"],["dc.identifier.isi","000349391400011"],["dc.identifier.pmid","25325933"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11155"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37864"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-072X"],["dc.relation.issn","0302-8933"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The effect of skin fatty acids on Staphylococcus aureus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Nagel, Alexandra"],["dc.contributor.author","Nesemann, Kai"],["dc.contributor.author","Höfer, Annalena M."],["dc.contributor.author","Bastakis, Emmanouil"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Stanley, Claire E."],["dc.contributor.author","Stöckli, Martina"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Hoff, Katharina J."],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","deMello, Andrew J."],["dc.contributor.author","Künzler, Markus"],["dc.contributor.author","Haney, Cara H."],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.date.accessioned","2021-07-05T14:57:54Z"],["dc.date.available","2021-07-05T14:57:54Z"],["dc.date.issued","2021"],["dc.description.abstract","Phytopathogenic Verticillia cause Verticillium wilt on numerous economically important crops. Plant infection begins at the roots, where the fungus is confronted with rhizosphere inhabiting bacteria. The effects of different fluorescent pseudomonads, including some known biocontrol agents of other plant pathogens, on fungal growth of the haploid Verticillium dahliae and/or the amphidiploid Verticillium longisporum were compared on pectin-rich medium, in microfluidic interaction channels, allowing visualization of single hyphae, or on Arabidopsis thaliana roots. We found that the potential for formation of bacterial lipopeptide syringomycin resulted in stronger growth reduction effects on saprophytic Aspergillus nidulans compared to Verticillium spp. A more detailed analyses on bacterial-fungal co-cultivation in narrow interaction channels of microfluidic devices revealed that the strongest inhibitory potential was found for Pseudomonas protegens CHA0, with its inhibitory potential depending on the presence of the GacS/GacA system controlling several bacterial metabolites. Hyphal tip polarity was altered when V. longisporum was confronted with pseudomonads in narrow interaction channels, resulting in a curly morphology instead of straight hyphal tip growth. These results support the hypothesis that the fungus attempts to evade the bacterial confrontation. Alterations due to co-cultivation with bacteria could not only be observed in fungal morphology but also in fungal transcriptome. P. protegens CHA0 alters transcriptional profiles of V. longisporum during 2 h liquid media co-cultivation in pectin-rich medium. Genes required for degradation of and growth on the carbon source pectin were down-regulated, whereas transcripts involved in redox processes were up-regulated. Thus, the secondary metabolite mediated effect of Pseudomonas isolates on Verticillium species results in a complex transcriptional response, leading to decreased growth with precautions for self-protection combined with the initiation of a change in fungal growth direction. This interplay of bacterial effects on the pathogen can be beneficial to protect plants from infection, as shown with A . thaliana root experiments. Treatment of the roots with bacteria prior to infection with V. dahliae resulted in a significant reduction of fungal root colonization. Taken together we demonstrate how pseudomonads interfere with the growth of Verticillium spp. and show that these bacteria could serve in plant protection."],["dc.description.abstract","Phytopathogenic Verticillia cause Verticillium wilt on numerous economically important crops. Plant infection begins at the roots, where the fungus is confronted with rhizosphere inhabiting bacteria. The effects of different fluorescent pseudomonads, including some known biocontrol agents of other plant pathogens, on fungal growth of the haploid Verticillium dahliae and/or the amphidiploid Verticillium longisporum were compared on pectin-rich medium, in microfluidic interaction channels, allowing visualization of single hyphae, or on Arabidopsis thaliana roots. We found that the potential for formation of bacterial lipopeptide syringomycin resulted in stronger growth reduction effects on saprophytic Aspergillus nidulans compared to Verticillium spp. A more detailed analyses on bacterial-fungal co-cultivation in narrow interaction channels of microfluidic devices revealed that the strongest inhibitory potential was found for Pseudomonas protegens CHA0, with its inhibitory potential depending on the presence of the GacS/GacA system controlling several bacterial metabolites. Hyphal tip polarity was altered when V. longisporum was confronted with pseudomonads in narrow interaction channels, resulting in a curly morphology instead of straight hyphal tip growth. These results support the hypothesis that the fungus attempts to evade the bacterial confrontation. Alterations due to co-cultivation with bacteria could not only be observed in fungal morphology but also in fungal transcriptome. P. protegens CHA0 alters transcriptional profiles of V. longisporum during 2 h liquid media co-cultivation in pectin-rich medium. Genes required for degradation of and growth on the carbon source pectin were down-regulated, whereas transcripts involved in redox processes were up-regulated. Thus, the secondary metabolite mediated effect of Pseudomonas isolates on Verticillium species results in a complex transcriptional response, leading to decreased growth with precautions for self-protection combined with the initiation of a change in fungal growth direction. This interplay of bacterial effects on the pathogen can be beneficial to protect plants from infection, as shown with A . thaliana root experiments. Treatment of the roots with bacteria prior to infection with V. dahliae resulted in a significant reduction of fungal root colonization. Taken together we demonstrate how pseudomonads interfere with the growth of Verticillium spp. and show that these bacteria could serve in plant protection."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3389/fmicb.2021.652468"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87766"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-441"],["dc.relation.eissn","1664-302X"],["dc.relation.orgunit","Abteilung Molekulare Mikrobiologie & Genetik"],["dc.rights","CC BY 4.0"],["dc.title","Pseudomonas Strains Induce Transcriptional and Morphological Changes and Reduce Root Colonization of Verticillium spp."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Leonard, Miriam"],["dc.contributor.author","Kühn, Anika"],["dc.contributor.author","Harting, Rebekka"],["dc.contributor.author","Maurus, Isabel"],["dc.contributor.author","Nagel, Alexandra"],["dc.contributor.author","Starke, Jessica"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Feussner, Kirstin"],["dc.contributor.author","Feussner, Ivo"],["dc.contributor.author","Kaever, Alexander"],["dc.contributor.author","Landesfeind, Manuel"],["dc.contributor.author","Morgenstern, Burkhard"],["dc.contributor.author","Becher, Dörte"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2021-04-14T08:23:50Z"],["dc.date.available","2021-04-14T08:23:50Z"],["dc.date.issued","2020"],["dc.description.abstract","Verticillia cause a vascular wilt disease affecting a broad range of economically valuable crops. The fungus enters its host plants through the roots and colonizes the vascular system. It requires extracellular proteins for a successful plant colonization. The exoproteomes of the allodiploid Verticillium longisporum upon cultivation in different media or xylem sap extracted from its host plant Brassica napus were compared. Secreted fungal proteins were identified by label free liquid chromatography-tandem mass spectrometry screening. V. longisporum induced two main secretion patterns. One response pattern was elicited in various non-plant related environments. The second pattern includes the exoprotein responses to the plant-related media, pectin-rich simulated xylem medium and pure xylem sap, which exhibited similar but additional distinct features. These exoproteomes include a shared core set of 221 secreted and similarly enriched fungal proteins. The pectin-rich medium significantly induced the secretion of 143 proteins including a number of pectin degrading enzymes, whereas xylem sap triggered a smaller but unique fungal exoproteome pattern with 32 enriched proteins. The latter pattern included proteins with domains of known pathogenicity factors, metallopeptidases and carbohydrate-active enzymes. The most abundant proteins of these different groups are the necrosis and ethylene inducing-like proteins Nlp2 and Nlp3, the cerato-platanin proteins Cp1 and Cp2, the metallopeptidases Mep1 and Mep2 and the carbohydrate-active enzymes Gla1, Amy1 and Cbd1. Their pathogenicity contribution was analyzed in the haploid parental strain V. dahliae. Deletion of the majority of the corresponding genes caused no phenotypic changes during ex planta growth or invasion and colonization of tomato plants. However, we discovered that the MEP1, NLP2, and NLP3 deletion strains were compromised in plant infections. Overall, our exoproteome approach revealed that the fungus induces specific secretion responses in different environments. The fungus has a general response to non-plant related media whereas it is able to fine-tune its exoproteome in the presence of plant material. Importantly, the xylem sap-specific exoproteome pinpointed Nlp2 and Nlp3 as single effectors required for successful V. dahliae colonization."],["dc.identifier.doi","10.3389/fmicb.2020.01876"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17508"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81068"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.publisher","Frontiers Media S.A."],["dc.relation.eissn","1664-302X"],["dc.rights","http://creativecommons.org/licenses/by/4.0/"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Verticillium longisporum Elicits Media-Dependent Secretome Responses With Capacity to Distinguish Between Plant-Related Environments"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Bioinformatics"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Suhr, M."],["dc.contributor.author","Lehmann, C."],["dc.contributor.author","Bauer, C. R."],["dc.contributor.author","Bender, T."],["dc.contributor.author","Knopp, C."],["dc.contributor.author","Freckmann, L."],["dc.contributor.author","Öst Hansen, B."],["dc.contributor.author","Henke, C."],["dc.contributor.author","Aschenbrandt, G."],["dc.contributor.author","Kühlborn, L. K."],["dc.contributor.author","Rheinländer, S."],["dc.contributor.author","Weber, L."],["dc.contributor.author","Marzec, B."],["dc.contributor.author","Hellkamp, M."],["dc.contributor.author","Wieder, P."],["dc.contributor.author","Sax, U."],["dc.contributor.author","Kusch, H."],["dc.contributor.author","Nussbeck, S. Y."],["dc.date.accessioned","2021-04-14T08:32:27Z"],["dc.date.available","2021-04-14T08:32:27Z"],["dc.date.issued","2020"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1186/s12859-020-03928-1"],["dc.identifier.pmid","33334310"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17711"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83924"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/374"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/132"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | INF: Unterstützung der SFB 1002 Forschungsdatenintegration, -visualisierung und -nachnutzung"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | Z03: Synthetische genetische Analyse, automatisierte Mikroskopie und Bildanalyse"],["dc.relation.eissn","1471-2105"],["dc.relation.orgunit","Gesellschaft für wissenschaftliche Datenverarbeitung"],["dc.relation.workinggroup","RG Nußbeck"],["dc.relation.workinggroup","RG Schwappach (Membrane Protein Biogenesis)"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Menoci: lightweight extensible web portal enhancing data management for biomedical research projects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e70669"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PloS one"],["dc.bibliographiccitation.lastpage","e70669"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Fuchs, Stephan"],["dc.contributor.author","Zühlke, Daniela"],["dc.contributor.author","Pané-Farré, Jan"],["dc.contributor.author","Kusch, Harald"],["dc.contributor.author","Wolf, Carmen"],["dc.contributor.author","Reiß, Swantje"],["dc.contributor.author","Binh, Le Thi Nguyen"],["dc.contributor.author","Albrecht, Dirk"],["dc.contributor.author","Riedel, Katharina"],["dc.contributor.author","Hecker, Michael"],["dc.contributor.author","Engelmann, Susanne"],["dc.date.accessioned","2019-07-09T11:40:11Z"],["dc.date.available","2019-07-09T11:40:11Z"],["dc.date.issued","2013"],["dc.description.abstract","Gel-based proteomics is a powerful approach to study the physiology of Staphylococcus aureus under various growth restricting conditions. We analyzed 679 protein spots from a reference 2-dimensional gel of cytosolic proteins of S. aureus COL by mass spectrometry resulting in 521 different proteins. 4,692 time dependent protein synthesis profiles were generated by exposing S. aureus to nine infection-related stress and starvation stimuli (H2O2, diamide, paraquat, NO, fermentation, nitrate respiration, heat shock, puromycin, mupirocin). These expression profiles are stored in an online resource called Aureolib (http://www.aureolib.de). Moreover, information on target genes of 75 regulators and regulatory elements were included in the database. Cross-comparisons of this extensive data collection of protein synthesis profiles using the tools implemented in Aureolib lead to the identification of stress and starvation specific marker proteins. Altogether, 226 protein synthesis profiles showed induction ratios of 2.5-fold or higher under at least one of the tested conditions with 157 protein synthesis profiles specifically induced in response to a single stimulus. The respective proteins might serve as marker proteins for the corresponding stimulus. By contrast, proteins whose synthesis was increased or repressed in response to more than four stimuli are rather exceptional. The only protein that was induced by six stimuli is the universal stress protein SACOL1759. Most strikingly, cluster analyses of synthesis profiles of proteins differentially synthesized under at least one condition revealed only in rare cases a grouping that correlated with known regulon structures. The most prominent examples are the GapR, Rex, and CtsR regulon. In contrast, protein synthesis profiles of proteins belonging to the CodY and σ(B) regulon are widely distributed. In summary, Aureolib is by far the most comprehensive protein expression database for S. aureus and provides an essential tool to decipher more complex adaptation processes in S. aureus during host pathogen interaction."],["dc.identifier.doi","10.1371/journal.pone.0070669"],["dc.identifier.fs","597423"],["dc.identifier.pmid","23967085"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10724"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58109"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1932-6203"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.mesh","Adaptation, Biological"],["dc.subject.mesh","Bacterial Proteins"],["dc.subject.mesh","Cluster Analysis"],["dc.subject.mesh","Computational Biology"],["dc.subject.mesh","Databases, Genetic"],["dc.subject.mesh","Gene Expression Regulation, Bacterial"],["dc.subject.mesh","Hydrogen Peroxide"],["dc.subject.mesh","Proteome"],["dc.subject.mesh","Proteomics"],["dc.subject.mesh","Signal Transduction"],["dc.subject.mesh","Staphylococcus aureus"],["dc.subject.mesh","Stress, Physiological"],["dc.subject.mesh","User-Computer Interface"],["dc.title","Aureolib - a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]