Mansouri, Ahmed

[["dc.bibliographiccitation.firstpage","396"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Stem Cells"],["dc.bibliographiccitation.lastpage","404"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Baier, Paul Christian"],["dc.contributor.author","Schindehutte, J."],["dc.contributor.author","Thinyane, K."],["dc.contributor.author","Flugge, G."],["dc.contributor.author","Fuchs, E."],["dc.contributor.author","Mansouri, Ahmed"],["dc.contributor.author","Paulus, Walter J."],["dc.contributor.author","Gruss, P."],["dc.contributor.author","Trenkwalder, Claudia"],["dc.date.accessioned","2018-11-07T10:53:15Z"],["dc.date.available","2018-11-07T10:53:15Z"],["dc.date.issued","2004"],["dc.description.abstract","Objective. Transplantation of fetal mesencephalic cells into the striatum has been performed in about 350 patients with Parkinson's disease and has been intensively studied in rat models of Parkinson's disease. Limited access to this material has shifted the focus toward embryonic stem (ES) cells. The grafting of undifferentiated ES cells to 6-hydroxy-dopamine (6-OHDA)-lesioned rats leads to behavioral improvements but may induce teratoma-like structures. This risk might be avoided by using more differentiated ES cells. In this study, we aimed to investigate differentiated mouse ES cells regarding their in vivo development and fate after transplantation in the striatum in the 6-OHDA rat model and the behavioral changes induced after transplantation. Methods. Mouse ES cells were differentiated on PA6 feeder cells for 14 days before grafting. Twenty to twenty-five percent of the neurons obtained were positive for tyrosine-hydroxylase (TH). PKH26-labeled cells were transplanted in the striata of unilaterally 6-OHDA-lesioned rats. Results. Direct PKH26 fluorescence visualization and TH staining proved the existence of cell deposits in the striata of all grafted animals, indicating cell survival for at least 5 weeks posttransplantation. There was no evidence of tumor formation. Immunocytochemical staining showed glial immunoreactivity surrounding the grafted cell deposits, probably inhibiting axonal outgrowth into the surrounding host tissue. There was a significant reduction in amphetamine-induced rotational behavior seen in grafted animals, which was not observed in sham-operated animals. Conclusions. The findings of this study suggest that the amphetamine-induced rotational behavioral test without histological confirmation is not proof of morphological integration with axonal outgrowth within the first 4 weeks posttransplantation."],["dc.identifier.doi","10.1634/stemcells.22-3-396"],["dc.identifier.isi","000221869600019"],["dc.identifier.pmid","15153616"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49315"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1066-5099"],["dc.title","Behavioral changes in unilaterally 6-hydroxy-dopamine lesioned rats after transplantation of differentiated mouse embryonic stem cells without morphological integration"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","80"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Brain Research"],["dc.bibliographiccitation.lastpage","87"],["dc.bibliographiccitation.volume","1045"],["dc.contributor.author","Thinyane, K."],["dc.contributor.author","Baier, Paul Christian"],["dc.contributor.author","Schindehutte, J."],["dc.contributor.author","Mansouri, Ahmed"],["dc.contributor.author","Paulus, Walter J."],["dc.contributor.author","Trenkwalder, Claudia"],["dc.contributor.author","Flugge, G."],["dc.contributor.author","Fuchs, E."],["dc.date.accessioned","2018-11-07T10:59:19Z"],["dc.date.available","2018-11-07T10:59:19Z"],["dc.date.issued","2005"],["dc.description.abstract","We transplanted mouse embryonic stem (ES) cells pre-differentiated on a PA6 feeder cell layer into the striatum of 6-hydroxydopamine hemi-lesioned adult rats and studied the fate of the grafted cells 1 and 5 weeks post-grafting. At both time points, ES cell grafts contained tyrosine hydroxylase positive (TH+) and 5-HT immunoreactive cells. Between 1 and 5 weeks, there was an enlargement of the grafts and an increase in number of TH+ cells although the differences between the two time points were not significant. The mean number of TH+ neurons per striatum was 330 73 after 1 week and 1220 400 after 5 weeks. Over the same time period, mean soma profile area of the TH+ neurons increased significantly by 25.2%. Neurites were longer after 5 weeks (by 24.9%), but the difference to 1 week post-grafting was not reliable. The percentage of TH+ somata without neurites increased from 6.7% after 1 week to 38.3% after 5 weeks (not significant). After 5 weeks, two out of fifteen graft recipients had tumors indicating that pre-differentiation of mouse embryonic stem cells using this differentiation protocol is not sufficient to prevent tumor formation. (C) 2005 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.brainres.2005.03.033"],["dc.identifier.isi","000229530300010"],["dc.identifier.pmid","15910765"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50671"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0006-8993"],["dc.title","Fate of pre-differentiated mouse embryonic stem cells transplanted in unilaterally 6-hydroxydopamine lesioned rats: Histological characterization of the grafted cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Stem Cells"],["dc.bibliographiccitation.lastpage","15"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Schindehutte, J."],["dc.contributor.author","Fukumitsu, H."],["dc.contributor.author","Collombat, P."],["dc.contributor.author","Griesel, G."],["dc.contributor.author","Brink, C."],["dc.contributor.author","Baier, Paul Christian"],["dc.contributor.author","Capecchi, M. R."],["dc.contributor.author","Mansouri, Ahmed"],["dc.date.accessioned","2018-11-07T08:48:30Z"],["dc.date.available","2018-11-07T08:48:30Z"],["dc.date.issued","2005"],["dc.description.abstract","Embryonic stem cells (ES) are pluripotent and may therefore serve as a source for the generation of specific cell types required for future therapies based on cell replacement. The isolation of defined cell populations from a certain lineage or tissue is a prerequisite for the analysis of the potential of such ES-derived cells in animal transplantation studies. Here, using the Cre/loxP system, we report the generation of murine ES cells conditionally expressing the hrGFP gene at the cell surface. Such ES cells can be differentiated in vitro into neurons displaying GFP activity in neurites. Transgenic mice derived from these ES cells permit the targeting of GFP-expression to specific tissues and provide material from the three germ layers suitable for molecular and biochemical analysis."],["dc.identifier.doi","10.1634/stemcells.2004-0163"],["dc.identifier.isi","000226259400002"],["dc.identifier.pmid","15625119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21224"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Alphamed Press"],["dc.relation.issn","1066-5099"],["dc.title","In vivo and in vitro tissue-specific expression of green fluorescent protein using the cre-lox system in mouse embryonic stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]