Franz, Monika

[["dc.bibliographiccitation.firstpage","448"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Viral Immunology"],["dc.bibliographiccitation.lastpage","457"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Stolte-Leeb, Nicole"],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Norley, Stephen"],["dc.contributor.author","Fagrouch, Zahra"],["dc.contributor.author","Heeney, Jonathan"],["dc.contributor.author","Franz, Monika"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.date.accessioned","2022-10-06T13:34:50Z"],["dc.date.available","2022-10-06T13:34:50Z"],["dc.date.issued","2006"],["dc.description.abstract","As part of a European multicenter study designed to determine the optimal combination and order of a mixed-modality vaccine against acquired immunodeficiency syndrome, rhesus monkeys received a combination of three different vectors, all expressing the same Simian Immunodeficiency Virus (SIV) genes followed by mucosal challenge with highly pathogenic SIV. In the study reported here, animals were primed with DNA followed by one booster immunization with Semliki Forest Virus (SFV) and two immunizations with modified Vaccinia Ankara (MVA). To address the relevance of mucosal immunization, we compared systemic versus a combination of systemic and mucosal antigen application. Although all vaccinees became infected after intrarectal challenge with SIV, most (six of eight) were protected from profound loss of CD4+ cells. In addition, vaccinees showed lower viral loads than did controls (p < 0.05). Overall, these protective effects were more pronounced in those animals whose schedule included immunization via the mucosa. In summary, the vaccine regimen used here achieved one important criterion of efficacy: the suppression of disease development as indicated by conservation of CD4+ cells."],["dc.identifier.doi","10.1089/vim.2006.19.448"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115988"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1557-8976"],["dc.relation.issn","0882-8245"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Sustained Conservation of CD4 + T Cells in Multiprotein Triple Modality-Immunized Rhesus Macaques after Intrarectal Challenge with Simian Immunodeficiency Virus"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4469"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Journal of Virology"],["dc.bibliographiccitation.lastpage","4481"],["dc.bibliographiccitation.volume","80"],["dc.contributor.author","Brenner, Matthias"],["dc.contributor.author","Münch, Jan"],["dc.contributor.author","Schindler, Michael"],["dc.contributor.author","Wildum, Steffen"],["dc.contributor.author","Stolte, Nicole"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Fuchs, Dietmar"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Franz, Monika"],["dc.contributor.author","Heeney, Jonathan"],["dc.contributor.author","Kirchhoff, Frank"],["dc.date.accessioned","2022-10-06T13:25:46Z"],["dc.date.available","2022-10-06T13:25:46Z"],["dc.date.issued","2006"],["dc.description.abstract","ABSTRACT\n \n Point mutations in SIVmac239 Nef disrupting CD4 downmodulation and enhancement of virion infectivity attenuate viral replication in acutely infected rhesus macaques, but changes selected later in infection fully restore Nef function (A. J. Iafrate et al., J. Virol. 74:9836-9844, 2000). To further evaluate the relevance of these Nef functions for viral persistence and disease progression, we analyzed an SIVmac239 Nef mutant containing a deletion of amino acids Q64 to N67 (Δ64-67Nef). This mutation inactivates the N-distal AP-2 clathrin adaptor binding element and disrupts the abilities of Nef to downregulate CD4, CD28 and CXCR4 and to stimulate viral replication in vitro. However, it does not impair the downmodulation of CD3 and class I major histocompatibility complex (MHC-I) or MHC-II and the upregulation of the MHC-II-associated invariant chain, and it has only a moderate effect on the enhancement of virion infectivity. Replication of the Δ64-67Nef variant in acutely infected macaques was intermediate between grossly\n nef\n -deleted and wild-type SIVmac239. Subsequently, three of six macaques developed moderate to high viral loads and developed disease, whereas the remaining animals efficiently controlled SIV replication and showed a more attenuated clinical course of infection. Sequence analysis revealed that the deletion in\n nef\n was not repaired in any of these animals. However, some changes that slightly enhanced the ability of Nef to downmodulate CD4 and moderately increased Nef-mediated enhancement of viral replication and infectivity in vitro were observed in macaques developing high viral loads. Our results imply that both the Nef functions that were disrupted by the Δ64-67 mutation and the activities that remained intact contribute to viral pathogenicity."],["dc.identifier.doi","10.1128/JVI.80.9.4469-4481.2006"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114910"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.eissn","1098-5514"],["dc.relation.issn","0022-538X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.rights.uri","https://journals.asm.org/non-commercial-tdm-license"],["dc.title","Importance of the N-Distal AP-2 Binding Element in Nef for Simian Immunodeficiency Virus Replication and Pathogenicity in Rhesus Macaques"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1811"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Vaccine"],["dc.bibliographiccitation.lastpage","1820"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Suh, You S."],["dc.contributor.author","Park, Ki S."],["dc.contributor.author","Sauermann, Ulrike"],["dc.contributor.author","Franz, Monika"],["dc.contributor.author","Norley, Stephen"],["dc.contributor.author","Wilfingseder, Doris"],["dc.contributor.author","Stoiber, Heribert"],["dc.contributor.author","Fagrouch, Zahra"],["dc.contributor.author","Heeney, Jonathan"],["dc.contributor.author","Hunsmann, Gerhard"],["dc.contributor.author","Sung, Young C."],["dc.date.accessioned","2022-10-06T13:33:26Z"],["dc.date.available","2022-10-06T13:33:26Z"],["dc.date.issued","2006"],["dc.identifier.doi","10.1016/j.vaccine.2005.10.026"],["dc.identifier.pii","S0264410X05010637"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/115631"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-602"],["dc.relation.issn","0264-410X"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Reduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]