Budde, Holger

[["dc.bibliographiccitation.firstpage","58"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Perfusion"],["dc.bibliographiccitation.lastpage","66"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Budde, Holger"],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Vormfelde, Steffen"],["dc.contributor.author","Tirilomis, Theodor"],["dc.contributor.author","Friedrich, Martin G."],["dc.date.accessioned","2020-12-10T18:38:24Z"],["dc.date.available","2020-12-10T18:38:24Z"],["dc.date.issued","2019-01"],["dc.description.abstract","Background: Re-transfusion of autologous blood is an important aspect of intraoperative blood management. Hemolysis and platelet dysfunction due to turbulence in the blood suction system strongly impede later usage of suction blood for re-transfusion. The aim of this study was to analyze the effects of a novel surgical-blood suction system with an automatic control setup for minimization of turbulence in the blood flow. Methods: We compared the turbulence-controlled suction system (TCSS) with a conventional suction system and untreated control blood in vitro. Blood cell counts, hemolysis levels according to free hemoglobin (fHb) and platelet function were analyzed to determine the integrity of the suction blood. Results: In the conventional suction system, we found a strong increase of the fHb levels. In contrast, erythrocyte integrity was almost completely preserved when using the TCSS. We obtained similar results regarding platelet function. The expression of platelet glycoproteins, such as GP IIb/IIIa and P-selectin, native or after stimulation with ADP, were markedly impaired by the conventional system, but not by the TCSS. In addition, platelet aggregometry revealed significant platelet dysfunction in conventional suction blood, but less aggregation impairments were present in blood samples from the TCSS. Conclusion: Our findings on an in vitro assessment show major improvements in red blood cell integrity and platelet function of suction blood when using the TCSS compared to a conventional suction system. These results reflect a significant benefit for autologous re-transfusion. We suggest testing the TCSS in surgery for clinical evaluation."],["dc.identifier.doi","10.1177/0267659118790915"],["dc.identifier.eissn","1477-111X"],["dc.identifier.issn","0267-6591"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77305"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","SAGE Publications"],["dc.relation.eissn","1477-111X"],["dc.relation.issn","0267-6591"],["dc.title","The effect of a novel turbulence-controlled suction system in the prevention of hemolysis and platelet dysfunction in autologous surgery blood"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e105896"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Budde, Holger"],["dc.contributor.author","Kolb, Susanne"],["dc.contributor.author","Tejedor, Laura Salinas"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Reichardt, Holger Michael"],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.date.accessioned","2018-11-07T09:36:27Z"],["dc.date.available","2018-11-07T09:36:27Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Graft-versus-host disease (GvHD) is a major challenge after hematopoietic stem cell transplantation but treatment options for patients are still limited. In many cases first-line treatment with glucocorticoids is not successful. Among second-line therapies the extracorporeal photopheresis (ECP) is frequently performed, due to induction of selective tolerance instead of general immunosuppression. However, for some patients with severe acute GvHD the leukapheresis step of the ECP procedure is physically exhausting and limits the number of ECP cycles. Methods: We hypothesized that leukocytes from healthy cell donors could be used as a replacement for ECP leukocytes gained from the GvHD patient. For this purpose we used a well established mouse model of acute GvHD. The ECP therapy was based on cells with the genetic background of the initial donor of the stem cell transplantation. As a precondition we developed a protocol representing conventional ECP in mice equivalent to clinical used ECP setup. Results: We could demonstrate that conventional, clinically derived ECP setup is able to alleviate acute GvHD. By using leukocytes obtained from healthy mice with the bone marrow donor's genetic background we could not observe a statistically significant therapeutic effect. Conclusions: Conventional human ECP setup is effective in the mouse model of severe acute GvHD. In addition we could not prove that ECP cells from healthy mice with bone marrow donor's genetic background are as effective as ECP cells derived from GvHD mice. Based on our findings, new questions arise for further studies, in which the cellular characteristics for ECP mediated immune tolerance are a matter of investigation."],["dc.description.sponsorship","Deutsche Jose Carreras Leukamie Stiftung e.V [DJCLS R 12/34]"],["dc.identifier.doi","10.1371/journal.pone.0105896"],["dc.identifier.isi","000341230600094"],["dc.identifier.pmid","25148404"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10790"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32622"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Modified Extracorporeal Photopheresis with Cells from a Healthy Donor for Acute Graft-versus-Host Disease in a Mouse Model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Central European Journal of Immunology"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Budde, Holger"],["dc.contributor.author","Berntsch, Ulrike"],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.date.accessioned","2018-11-07T10:28:37Z"],["dc.date.available","2018-11-07T10:28:37Z"],["dc.date.issued","2017"],["dc.description.abstract","Extracorporeal photopheresis (ECP) is an important second-line therapy for graft-versus-host disease. A central therapeutic mechanism is the induction of immune tolerance through apoptosis in patient's leukocytes, caused by ex vivo incubation with 8-methoxypsoralen (8-MOP) and subsequent UVA irradiation. We hypothesized that different 8-MOP incubation times and an additional 8-MOP removal step could influence the apoptosis kinetics of leukocytes in general and in particular could lead to different apoptotic levels in the leukocyte subpopulations. After 8-MOP/UVA treatment of human leukocytes, cells were cultured and the percentage of annexin V positive cells from several leukocyte subpopulations was determined. Only regulatory T cells (Tregs) were relatively resistant to 8-MOP/UVA induced apoptosis. When cells were incubated for 30 minutes with 8-MOP prior to UVA exposure, higher percentages of annexin V positive cells were detected on day 1 and day 2 after treatment. Removal of 8-MOP after UVA exposure caused no significant changes in the apoptosis kinetics during the 72 h culture period compared with unwashed cells. The results of our in vitro study indicate that it could be possible to adjust the apoptosis kinetics via modulation of the 8-MOP incubation time. In further in vivo studies it should be elucidated to which extent different apoptosis kinetics influence the therapeutic effect of ECP since steady-state apoptosis levels are probably important for establishing a long lasting immune tolerance. Furthermore we found that Tregs, according to their well-known tolerogenic function, are more resistant to apoptosis after 8-MOP/UVA treatment compared to GvHD inducing T cell populations."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.5114/ceji.2017.67312"],["dc.identifier.isi","000401601400001"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14479"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43462"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Termedia Publishing House Ltd"],["dc.relation.issn","1644-4124"],["dc.relation.issn","1426-3912"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","In vitro effects of different 8-methoxypsoralen treatment protocols for extracorporeal photopheresis on mononuclear cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","023"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Jacobs Journal of Hematology"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Budde, Holger"],["dc.contributor.author","Thieringer, Lena"],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Legler, Tobias J."],["dc.date.accessioned","2019-07-10T08:12:01Z"],["dc.date.available","2019-07-10T08:12:01Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Graft-versus-host disease (GvHD) is still one of the most challenging complications after allogeneic hematopoietic stem cell transplantation. Steroid-resistant or steroid-refractory patients with acute GvHD have limited therapeutic options and there is a strong demand in clinics for new therapeutic approaches. Due to the published anti-inflammatory effects of heparin, we hypothesized that either unfractionated (UFH) or low-molecular-weight heparin (LMWH) could be a novel treatment option for patients with acute GvHD. Methods We performed dose finding studies with both UFH and LMWH via intravenous (iv) and subcutaneous (sc) routes of application in a mouse model of acute GvHD. Survival rates, clinical GvHD score, CD4+ and CD8+ T cell levels were determined. Results Iv administration of UFH caused slightly but no statistically improved survival rates or clinical scores of the GvHD animals. Neither sc administration of UFH, nor sc or iv administration of LMWH was linked to improved survival or clinical GvHD score. CD4+ and CD8+ T cell levels were not influenced by UFH or LMWH treatment. Conclusion UFH seems to be more effective than LMWH. However, the therapeutic effect of UFH on acute GvHD is limited and heparin alone is probably not sufficient for the therapy of acute GvHD."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2015"],["dc.format.extent","6"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13468"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60844"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.issn","2380-1646"],["dc.rights.access","openAccess"],["dc.subject","GvHD; Heparins; Immune-Suppression; Bone Marrow Transplantation"],["dc.subject.ddc","610"],["dc.title","Anti-Inflammatory Effects of Heparin: Do they Influence Murine Acute GvHD?"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]