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Zhang, Wei
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Zhang, Wei
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Zhang, Wei
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Zhang, W.
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2022Journal Article [["dc.bibliographiccitation.artnumber","S0929139322000117"],["dc.bibliographiccitation.firstpage","104395"],["dc.bibliographiccitation.journal","Applied Soil Ecology"],["dc.bibliographiccitation.volume","173"],["dc.contributor.author","Xiao, Dan"],["dc.contributor.author","He, Xunyang"],["dc.contributor.author","Wang, Guihong"],["dc.contributor.author","Xu, Xuechi"],["dc.contributor.author","Hu, Yajun"],["dc.contributor.author","Chen, Xiangbi"],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Su, Yirong"],["dc.contributor.author","Wang, Kelin"],["dc.contributor.author","Soromotin, Andrey V."],["dc.contributor.author","Kuzyakov, Yakov"],["dc.date.accessioned","2022-05-02T08:09:48Z"],["dc.date.available","2022-05-02T08:09:48Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1016/j.apsoil.2022.104395"],["dc.identifier.pii","S0929139322000117"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107468"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-561"],["dc.relation.issn","0929-1393"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Network analysis reveals bacterial and fungal keystone taxa involved in straw and soil organic matter mineralization"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]Details DOI2022Journal Article [["dc.bibliographiccitation.artnumber","S0016706121006455"],["dc.bibliographiccitation.firstpage","115565"],["dc.bibliographiccitation.journal","Geoderma"],["dc.bibliographiccitation.volume","407"],["dc.contributor.author","Zheng, H."],["dc.contributor.author","Guber, A.K."],["dc.contributor.author","Kuzyakov, Y."],["dc.contributor.author","Zhang, W."],["dc.contributor.author","Kravchenko, A.N."],["dc.date.accessioned","2022-01-11T14:05:35Z"],["dc.date.available","2022-01-11T14:05:35Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1016/j.geoderma.2021.115565"],["dc.identifier.pii","S0016706121006455"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97698"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation.issn","0016-7061"],["dc.title","Plant species and plant neighbor identity affect associations between plant assimilated C inputs and soil pores"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]Details DOI2013Book Chapter [["dc.bibliographiccitation.firstpage","523"],["dc.bibliographiccitation.lastpage","587"],["dc.contributor.author","Ho Yoon, Jung"],["dc.contributor.author","Jung, Hyung-Suk"],["dc.contributor.author","Hwan Lee, Min"],["dc.contributor.author","Hwan Kim, Gun"],["dc.contributor.author","Ji Song, Seul"],["dc.contributor.author","Yeong Seok, Jun"],["dc.contributor.author","Jean Yoon, Kyung"],["dc.contributor.author","Seong Hwang, Cheol"],["dc.contributor.author","Besland, M.-P."],["dc.contributor.author","Tranchant, J."],["dc.contributor.author","Souchier, E."],["dc.contributor.author","Moreau, P."],["dc.contributor.author","Salmon, S."],["dc.contributor.author","Corraze, B."],["dc.contributor.author","Janod, E."],["dc.contributor.author","Cario, L."],["dc.contributor.author","Zazpe, Raúl"],["dc.contributor.author","Ungureanu, Mariana"],["dc.contributor.author","Llopis, Roger"],["dc.contributor.author","Golmar, Federico"],["dc.contributor.author","Stoliar, Pablo"],["dc.contributor.author","Casanova, Félix"],["dc.contributor.author","Eduardo Hueso, Luis"],["dc.contributor.author","Hermes, C."],["dc.contributor.author","Wimmer, M."],["dc.contributor.author","Menzel, S."],["dc.contributor.author","Fleck, K."],["dc.contributor.author","Rana, V."],["dc.contributor.author","Salinga, M."],["dc.contributor.author","Böttger, U."],["dc.contributor.author","Bruchhaus, R."],["dc.contributor.author","Wuttig, M."],["dc.contributor.author","Waser, R."],["dc.contributor.author","Lentz, F."],["dc.contributor.author","Hermes, C."],["dc.contributor.author","Rösgen, B."],["dc.contributor.author","Selle, T."],["dc.contributor.author","Bruchhaus, R."],["dc.contributor.author","Rana, V."],["dc.contributor.author","Waser, R."],["dc.contributor.author","Marchewka, Astrid"],["dc.contributor.author","Menzel, Stephan"],["dc.contributor.author","Böttger, Ulrich"],["dc.contributor.author","Waser, Rainer"],["dc.contributor.author","Hoskins, Brian"],["dc.contributor.author","Alibart, Fabien"],["dc.contributor.author","Strukov, Dmitri"],["dc.contributor.author","Pellegrino, Luca"],["dc.contributor.author","Manca, Nicola"],["dc.contributor.author","Kanki, Teruo"],["dc.contributor.author","Tanaka, Hidekazu"],["dc.contributor.author","Biasotti, Michele"],["dc.contributor.author","Bellingeri, Emilio"],["dc.contributor.author","Sergio Siri, Antonio"],["dc.contributor.author","Marré, Daniele"],["dc.contributor.author","M. Padilha, Antonio Claudio"],["dc.contributor.author","Martini Dalpian, Gustavo"],["dc.contributor.author","Reily Rocha, Alexandre"],["dc.contributor.author","Prodromakis, Themistoklis"],["dc.contributor.author","Salaoru, Iulia"],["dc.contributor.author","Khiat, Ali"],["dc.contributor.author","Toumazou, Christopher"],["dc.contributor.author","Gale, Ella M."],["dc.contributor.author","Madhavan, A."],["dc.contributor.author","Adam, G."],["dc.contributor.author","Alibart, F."],["dc.contributor.author","Gao, L."],["dc.contributor.author","Strukov, D. B."],["dc.contributor.author","Wamwangi, D."],["dc.contributor.author","Welnic, W."],["dc.contributor.author","Wuttig, M."],["dc.contributor.author","Gholipour, Behrad"],["dc.contributor.author","Huang, Chung-Che"],["dc.contributor.author","Anastasopoulos, Alexandros"],["dc.contributor.author","Al-Saab, Feras"],["dc.contributor.author","Hayden, Brian E."],["dc.contributor.author","Hewak, Daniel W."],["dc.contributor.author","Lan, Rui"],["dc.contributor.author","Endo, Rie"],["dc.contributor.author","Kuwahara, Masashi"],["dc.contributor.author","Kobayashi, Yoshinao"],["dc.contributor.author","Susa, Masahiro"],["dc.contributor.author","Baumeister, Paul"],["dc.contributor.author","Wortmann, Daniel"],["dc.contributor.author","Blügel, Stefan"],["dc.contributor.author","Mazzarello, Riccardo"],["dc.contributor.author","Li, Yan"],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Ronneberger, Ider"],["dc.contributor.author","Simon, Ronnie"],["dc.contributor.author","Gallus, Jens"],["dc.contributor.author","Bessas, Dimitrios"],["dc.contributor.author","Sergueev, Ilya"],["dc.contributor.author","Wille, Hans-Christian"],["dc.contributor.author","Pierre Hermann, Raphaël"],["dc.contributor.author","Luckas, Jennifer"],["dc.contributor.author","Rausch, Pascal"],["dc.contributor.author","Krebs, Daniel"],["dc.contributor.author","Zalden, Peter"],["dc.contributor.author","Boltz, Janika"],["dc.contributor.author","Raty, Jean-Yves"],["dc.contributor.author","Salinga, Martin"],["dc.contributor.author","Longeaud, Christophe"],["dc.contributor.author","Wuttig, Matthias"],["dc.contributor.author","Kim, Haeri"],["dc.contributor.author","Kim, Dong-Wook"],["dc.contributor.author","Phark, Soo-Hyon"],["dc.contributor.author","Hong, Seungbum"],["dc.contributor.author","Park, C."],["dc.contributor.author","Herpers, A."],["dc.contributor.author","Bruchhaus, R."],["dc.contributor.author","Verbeeck, J."],["dc.contributor.author","Egoavil, R."],["dc.contributor.author","Borgatti, F."],["dc.contributor.author","Panaccione, G."],["dc.contributor.author","Offi, F."],["dc.contributor.author","Dittmann, R."],["dc.contributor.author","Clima, Sergiu"],["dc.contributor.author","Sankaran, Kiroubanand"],["dc.contributor.author","Mees, Maarten"],["dc.contributor.author","Yin Chen, Yang"],["dc.contributor.author","Goux, Ludovic"],["dc.contributor.author","Govoreanu, Bogdan"],["dc.contributor.author","Wouters, Dirk J."],["dc.contributor.author","Kittl, Jorge"],["dc.contributor.author","Jurczak, Malgorzata"],["dc.contributor.author","Pourtois, Geoffrey"],["dc.contributor.author","Calka, P."],["dc.contributor.author","Martinez, E."],["dc.contributor.author","Delaye, V."],["dc.contributor.author","Lafond, D."],["dc.contributor.author","Audoit, G."],["dc.contributor.author","Mariolle, D."],["dc.contributor.author","Chevalier, N."],["dc.contributor.author","Grampeix, H."],["dc.contributor.author","Cagli, C."],["dc.contributor.author","Jousseaume, V."],["dc.contributor.author","Guedj, C."],["dc.contributor.author","Shrestha, Pragya"],["dc.contributor.author","Ochia, Adaku"],["dc.contributor.author","Cheung, Kin. P."],["dc.contributor.author","Campbell, Jason"],["dc.contributor.author","Baumgart, Helmut"],["dc.contributor.author","Harris, Gary"],["dc.contributor.author","Scherff, Malte"],["dc.contributor.author","Meyer, Bjoern"],["dc.contributor.author","Scholz, Julius"],["dc.contributor.author","Hoffmann, Joerg"],["dc.contributor.author","Jooss, Christian"],["dc.contributor.author","Xiao, Bo"],["dc.contributor.author","Tada, Tomofumi"],["dc.contributor.author","Gu, Tingkun"],["dc.contributor.author","Tawara, Arihiro"],["dc.contributor.author","Watanabe, Satoshi"],["dc.contributor.author","Young, Tai-Fa"],["dc.contributor.author","Yang, Ya-Liang"],["dc.contributor.author","Chang, Ting-Chang"],["dc.contributor.author","Hsu, Kuang-Ting"],["dc.contributor.author","Chen, Chao-Yu"],["dc.contributor.author","Burkert, A"],["dc.contributor.author","Valov, I."],["dc.contributor.author","Staikov, G."],["dc.contributor.author","Waser, R."],["dc.contributor.author","van den Hurk, Jan"],["dc.contributor.author","Valov, Ilia"],["dc.contributor.author","Waser, Rainer"],["dc.contributor.author","Valov, Ilia"],["dc.contributor.author","Tappertzhofen, Stefan"],["dc.contributor.author","van der Hurk, Jan"],["dc.contributor.author","Waser, Rainer"],["dc.contributor.author","Adam, G."],["dc.contributor.author","Alibart, F."],["dc.contributor.author","Gao, L."],["dc.contributor.author","Hoskins, B."],["dc.contributor.author","Strukov, D. B."],["dc.contributor.author","Jean Yoon, Kyung"],["dc.contributor.author","Ji Song, Seul"],["dc.contributor.author","Kim, Gun Hwan"],["dc.contributor.author","Seok, Jun Yeong"],["dc.contributor.author","Ho Yoon, Jeong"],["dc.contributor.author","Seong Hwang, Cheol"],["dc.contributor.author","Yoon, Jung Ho"],["dc.contributor.author","Yoon, Kyung Jin"],["dc.contributor.author","Shuai, Yao"],["dc.contributor.author","Wu, Chuangui"],["dc.contributor.author","Zhang, Wanli"],["dc.contributor.author","Zhou, Shengqiang"],["dc.contributor.author","Bürger, Danilo"],["dc.contributor.author","Slesazeck, Stefan"],["dc.contributor.author","Mikolajick, Thomas"],["dc.contributor.author","Helm, Manfred"],["dc.contributor.author","Schmidt, Heidemarie"],["dc.contributor.author","Gale, Ella"],["dc.contributor.author","Pearson, David"],["dc.contributor.author","Kitson, Stephen"],["dc.contributor.author","Adamatzky, Andrew"],["dc.contributor.author","Costello, Ben de Lacy"],["dc.contributor.author","Lehtonen, Eero"],["dc.contributor.author","Poikonen, Jussi"],["dc.contributor.author","Laiho, Mika"],["dc.contributor.author","Kanerva, Pentti"],["dc.contributor.author","Lim, Hyungkwang"],["dc.contributor.author","Jang, Ho-won"],["dc.contributor.author","Jeong, Doo Seok"],["dc.contributor.author","Cao, Xun"],["dc.contributor.author","Jiang, Meng"],["dc.contributor.author","Zhang, Feng"],["dc.contributor.author","Liu, Xinjun"],["dc.contributor.author","Jin, Ping"],["dc.contributor.author","Zhang, Kai"],["dc.contributor.author","Tangirala, Madhavi"],["dc.contributor.author","Shrestha, Pragya"],["dc.contributor.author","Baumgart, Helmut"],["dc.contributor.author","Kittiwatanakul, Salinporn"],["dc.contributor.author","Lu, Jiwei"],["dc.contributor.author","Wolf, Stuart"],["dc.contributor.author","Pallem, Venkateswara"],["dc.contributor.author","Dussarrat, Christian"],["dc.contributor.author","Pinto, S."],["dc.contributor.author","Krishna, R."],["dc.contributor.author","Dias, C."],["dc.contributor.author","Pimentel, G."],["dc.contributor.author","Oliveira, G. N. P."],["dc.contributor.author","Teixeira, J. M."],["dc.contributor.author","Aguiar, P."],["dc.contributor.author","Titus, E."],["dc.contributor.author","Gracio, J."],["dc.contributor.author","Ventura, J."],["dc.contributor.author","Araujo, J. P."],["dc.contributor.editor","Heber, Jörg"],["dc.contributor.editor","Schlom, Darrell"],["dc.contributor.editor","Tokura, Yoshinori"],["dc.contributor.editor","Waser, Rainer"],["dc.contributor.editor","Wuttig, Matthias"],["dc.date.accessioned","2021-12-08T12:29:05Z"],["dc.date.available","2021-12-08T12:29:05Z"],["dc.date.issued","2013"],["dc.identifier.doi","10.1002/9783527667703.ch67"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/95954"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-476"],["dc.publisher","Wiley-VCH Verlag GmbH & Co. KGaA"],["dc.publisher.place","Weinheim, Germany"],["dc.relation.eisbn","9783527667703"],["dc.relation.isbn","9783527411917"],["dc.relation.ispartof","Frontiers in Electronic Materials : \n A Collection of Extended Abstracts of the Nature Conference Frontiers in Electronic Materials, June 17\n th\n to 20\n th\n 2012, Aachen, Germany"],["dc.title","Poster: Memristive Systems"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]Details DOI2000Journal Article [["dc.bibliographiccitation.firstpage","1279"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","The EMBO Journal"],["dc.bibliographiccitation.lastpage","1289"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Wei, S."],["dc.date.accessioned","2022-03-01T11:46:44Z"],["dc.date.available","2022-03-01T11:46:44Z"],["dc.date.issued","2000"],["dc.identifier.doi","10.1093/emboj/19.6.1279"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103778"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1460-2075"],["dc.title","Exocytotic mechanism studied by truncated and zero layer mutants of the C-terminus of SNAP-25"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]Details DOI2016Journal Article [["dc.bibliographiccitation.firstpage","593"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Molecular Cell"],["dc.bibliographiccitation.lastpage","607"],["dc.bibliographiccitation.volume","63"],["dc.contributor.author","Yamaguchi, Masaya"],["dc.contributor.author","VanderLinden, Ryan"],["dc.contributor.author","Weissmann, Florian"],["dc.contributor.author","Qiao, Renping"],["dc.contributor.author","Dube, Prakash"],["dc.contributor.author","Brown, Nicholas G."],["dc.contributor.author","Haselbach, David"],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Sidhu, Sachdev S."],["dc.contributor.author","Peters, Jan-Michael"],["dc.contributor.author","Schulman, Brenda A."],["dc.date.accessioned","2022-03-01T11:45:17Z"],["dc.date.available","2022-03-01T11:45:17Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1016/j.molcel.2016.07.003"],["dc.identifier.pii","S1097276516303306"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103275"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","1097-2765"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]Details DOI2018Journal Article [["dc.bibliographiccitation.firstpage","527"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Computer-Aided Civil and Infrastructure Engineering"],["dc.bibliographiccitation.lastpage","544"],["dc.bibliographiccitation.volume","33"],["dc.contributor.author","Li, Ruoxing"],["dc.contributor.author","Yuan, Yachao"],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Yuan, Yali"],["dc.date.accessioned","2018-09-06T10:46:16Z"],["dc.date.available","2018-09-06T10:46:16Z"],["dc.date.issued","2018"],["dc.description.abstract","Vision‐based autonomous inspection of concrete surface defects is crucial for efficient maintenance and rehabilitation of infrastructures and has become a research hot spot. However, most existing vision‐based inspection methods mainly focus on detecting one kind of defect in nearly uniform testing background where defects are relatively large and easily recognizable. But in the real‐world scenarios, multiple types of defects often occur simultaneously. And most of them occupy only small fractions of inspection images and are swamped in cluttered background, which easily leads to missed and false detections. In addition, the majority of the previous researches only focus on detecting defects but few of them pay attention to the geolocalization problem, which is indispensable for timely performing repair, protection, or reinforcement works. And most of them rely heavily on GPS for tracking the locations of the defects. However, this method is sometimes unreliable within infrastructures where the GPS signals are easily blocked, which causes a dramatic increase in searching costs. To address these limitations, we present a unified and purely vision‐based method denoted as defects detection and localization network, which can detect and classify various typical types of defects under challenging conditions while simultaneously geolocating the defects without requiring external localization sensors. We design a supervised deep convolutional neural network and propose novel training methods to optimize its performance on specific tasks. Extensive experiments show that the proposed method is effective with a detection accuracy of 80.7% and a localization accuracy of 86% at 0.41 s per image (at a scale of 1,200 pixels in the field test experiment), which is ideal for integration within intelligent autonomous inspection systems to provide support for practical applications."],["dc.identifier.doi","10.1111/mice.12351"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15669"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Unified Vision-Based Methodology for Simultaneous Concrete Defect Detection and Geolocalization"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]Details DOI2020Journal Article [["dc.bibliographiccitation.firstpage","639"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","IEEE ACM Transactions on Networking"],["dc.bibliographiccitation.lastpage","652"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Kulkarni, Sameer G."],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Hwang, Jinho"],["dc.contributor.author","Rajagopalan, Shriram"],["dc.contributor.author","Ramakrishnan, K. K."],["dc.contributor.author","Wood, Timothy"],["dc.contributor.author","Arumaithurai, Mayutan"],["dc.contributor.author","Fu, Xiaoming"],["dc.date.accessioned","2021-04-14T08:27:07Z"],["dc.date.available","2021-04-14T08:27:07Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1109/TNET.2020.2969971"],["dc.identifier.eissn","1558-2566"],["dc.identifier.issn","1063-6692"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82174"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1558-2566"],["dc.relation.issn","1063-6692"],["dc.title","NFVnice: Dynamic Backpressure and Scheduling for NFV Service Chains"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]Details DOI2019Journal Article [["dc.bibliographiccitation.firstpage","17280"],["dc.bibliographiccitation.issue","35"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","17289"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Watson, Edmond R."],["dc.contributor.author","Grace, Christy R. R."],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Miller, Darcie J."],["dc.contributor.author","Davidson, Iain F."],["dc.contributor.author","Prabu, J. Rajan"],["dc.contributor.author","Yu, Shanshan"],["dc.contributor.author","Bolhuis, Derek L."],["dc.contributor.author","Kulko, Elizaveta T."],["dc.contributor.author","Vollrath, Ronnald"],["dc.contributor.author","Schulman, Brenda A."],["dc.date.accessioned","2022-03-01T11:46:25Z"],["dc.date.available","2022-03-01T11:46:25Z"],["dc.date.issued","2019"],["dc.description.abstract","Ubiquitin (Ub)-mediated proteolysis is a fundamental mechanism used by eukaryotic cells to maintain homeostasis and protein quality, and to control timing in biological processes. Two essential aspects of Ub regulation are conjugation through E1-E2-E3 enzymatic cascades and recognition by Ub-binding domains. An emerging theme in the Ub field is that these 2 properties are often amalgamated in conjugation enzymes. In addition to covalent thioester linkage to Ub’s C terminus for Ub transfer reactions, conjugation enzymes often bind noncovalently and weakly to Ub at “exosites.” However, identification of such sites is typically empirical and particularly challenging in large molecular machines. Here, studying the 1.2-MDa E3 ligase anaphase-promoting complex/cyclosome (APC/C), which controls cell division and many aspects of neurobiology, we discover a method for identifying unexpected Ub-binding sites. Using a panel of Ub variants (UbVs), we identify a protein-based inhibitor that blocks Ub ligation to APC/C substrates in vitro and ex vivo. Biochemistry, NMR, and cryo-electron microscopy (cryo-EM) structurally define the UbV interaction, explain its inhibitory activity through binding the surface on the APC2 subunit that recruits the E2 enzyme UBE2C, and ultimately reveal that this APC2 surface is also a Ub-binding exosite with preference for K48-linked chains. The results provide a tool for probing APC/C activity, have implications for the coordination of K48-linked Ub chain binding by APC/C with the multistep process of substrate polyubiquitylation, and demonstrate the power of UbV technology for identifying cryptic Ub-binding sites within large multiprotein complexes."],["dc.description.abstract","Ubiquitin (Ub)-mediated proteolysis is a fundamental mechanism used by eukaryotic cells to maintain homeostasis and protein quality, and to control timing in biological processes. Two essential aspects of Ub regulation are conjugation through E1-E2-E3 enzymatic cascades and recognition by Ub-binding domains. An emerging theme in the Ub field is that these 2 properties are often amalgamated in conjugation enzymes. In addition to covalent thioester linkage to Ub’s C terminus for Ub transfer reactions, conjugation enzymes often bind noncovalently and weakly to Ub at “exosites.” However, identification of such sites is typically empirical and particularly challenging in large molecular machines. Here, studying the 1.2-MDa E3 ligase anaphase-promoting complex/cyclosome (APC/C), which controls cell division and many aspects of neurobiology, we discover a method for identifying unexpected Ub-binding sites. Using a panel of Ub variants (UbVs), we identify a protein-based inhibitor that blocks Ub ligation to APC/C substrates in vitro and ex vivo. Biochemistry, NMR, and cryo-electron microscopy (cryo-EM) structurally define the UbV interaction, explain its inhibitory activity through binding the surface on the APC2 subunit that recruits the E2 enzyme UBE2C, and ultimately reveal that this APC2 surface is also a Ub-binding exosite with preference for K48-linked chains. The results provide a tool for probing APC/C activity, have implications for the coordination of K48-linked Ub chain binding by APC/C with the multistep process of substrate polyubiquitylation, and demonstrate the power of UbV technology for identifying cryptic Ub-binding sites within large multiprotein complexes."],["dc.identifier.doi","10.1073/pnas.1902889116"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103661"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.rights.uri","https://www.pnas.org/site/aboutpnas/licenses.xhtml"],["dc.title","Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]Details DOI2021Journal Article Research Paper [["dc.bibliographiccitation.firstpage","323"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Trends in Neurosciences"],["dc.bibliographiccitation.lastpage","335"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Hehlert, Philip"],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Göpfert, Martin C."],["dc.date.accessioned","2021-04-14T08:26:49Z"],["dc.date.available","2021-04-14T08:26:49Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.tins.2020.11.001"],["dc.identifier.pmid","33257000"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82084"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/139"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","0166-2236"],["dc.relation.workinggroup","RG Göpfert"],["dc.title","Drosophila Mechanosensory Transduction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]Details DOI PMID PMC2015Journal Article [["dc.bibliographiccitation.firstpage","2969"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","The Journal of Physical Chemistry Letters"],["dc.bibliographiccitation.lastpage","2975"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Zheng, Kaibo"],["dc.contributor.author","Zhu, Qiushi"],["dc.contributor.author","Abdellah, Mohamed"],["dc.contributor.author","Messing, Maria E."],["dc.contributor.author","Zhang, Wei"],["dc.contributor.author","Generalov, Alexander"],["dc.contributor.author","Niu, Yuran"],["dc.contributor.author","Ribaud, Lynn"],["dc.contributor.author","Canton, Sophie E."],["dc.contributor.author","Pullerits, Tõnu"],["dc.date.accessioned","2021-06-01T10:50:28Z"],["dc.date.available","2021-06-01T10:50:28Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1021/acs.jpclett.5b01252"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86669"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","1948-7185"],["dc.relation.workinggroup","RG Techert (Structural Dynamics in Chemical Systems)"],["dc.title","Exciton Binding Energy and the Nature of Emissive States in Organometal Halide Perovskites"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]Details DOI