Bock, Hannes

[["dc.bibliographiccitation.firstpage","3285"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","3290"],["dc.bibliographiccitation.volume","93"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","von Middendorff, Claas"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Wenzel, Dirk"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:23Z"],["dc.date.available","2017-09-07T11:49:23Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm."],["dc.identifier.doi","10.1529/biophysj.107.112201"],["dc.identifier.gro","3143415"],["dc.identifier.isi","000250199300033"],["dc.identifier.pmid","17660318"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/927"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","161"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Applied Physics B Lasers and Optics"],["dc.bibliographiccitation.lastpage","165"],["dc.bibliographiccitation.volume","88"],["dc.contributor.author","Bock, H."],["dc.contributor.author","Geisler, C."],["dc.contributor.author","Wurm, C. A."],["dc.contributor.author","von Middendorff, C."],["dc.contributor.author","Jakobs, S."],["dc.contributor.author","Schönle, A."],["dc.contributor.author","Egner, A."],["dc.contributor.author","Hell, S. W."],["dc.contributor.author","Eggeling, C."],["dc.date.accessioned","2017-09-07T11:49:27Z"],["dc.date.available","2017-09-07T11:49:27Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate two-color far-field fluorescence microscopy with nanoscale spatial resolution based on the photoswitching of individual fluorescent markers. By enabling, recording, and disabling the emission of the reversibly switchable fluorescent protein rsFastLime and of the organic fluorophore cyanine5, we recorded two-color nanoscale images inside whole cells. The position of individual emitters was determined with a typical accuracy of 20 nm, which largely constitutes the lateral resolution of the system. Photoswitching in two-color colocalization experiments represents a major step towards the application of far-field fluorescence nanoscopy to the study of (biological) samples on the macromolecular level."],["dc.identifier.doi","10.1007/s00340-007-2729-0"],["dc.identifier.gro","3143472"],["dc.identifier.isi","000248054900001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/989"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0946-2171"],["dc.title","Two-color far-field fluorescence nanoscopy based on photoswitchable emitters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Applied Physics A: Materials Science & Processing"],["dc.bibliographiccitation.lastpage","226"],["dc.bibliographiccitation.volume","88"],["dc.contributor.author","Geisler, Claudia"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Middendorff, Claas von"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:26Z"],["dc.date.available","2017-09-07T11:49:26Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate nanoscale resolution in far-field optical microscopy based on photo-switching of molecules. By enabling, recording and disabling fluorescence from individual labels sequentially, the detection volume is reduced to the size of a single molecule and the diffraction limit is broken. Images of nanostructures milled into a coverslip and tagged by fluorescent proteins could be recorded at 50 nm resolution. Due to the fast and asynchronous image acquisition protocol used in these experiments, we were able to reduce acquisition times to similar to 2.5 min, which is two orders of magnitude lower than in previous implementations."],["dc.identifier.doi","10.1007/s00339-007-4144-0"],["dc.identifier.gro","3143454"],["dc.identifier.isi","000247255800001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/970"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0947-8396"],["dc.title","Resolution of λ /10 in fluorescence microscopy using fast single molecule photo-switching"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1003"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Microscopy research and technique"],["dc.bibliographiccitation.lastpage","1009"],["dc.bibliographiccitation.volume","70"],["dc.contributor.author","Eggeling, C."],["dc.contributor.author","Hilbert, M."],["dc.contributor.author","Bock, H."],["dc.contributor.author","Ringemann, C."],["dc.contributor.author","Hofmann, M."],["dc.contributor.author","Stiel, A. C."],["dc.contributor.author","Andresen, M."],["dc.contributor.author","Jakobs, S."],["dc.contributor.author","Egner, A."],["dc.contributor.author","Schönle, A."],["dc.contributor.author","Hell, S. W."],["dc.date.accessioned","2017-09-07T11:49:23Z"],["dc.date.available","2017-09-07T11:49:23Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate that photoswitchable markers enable fluorescence fluctuation spectroscopy at high molecular concentration. Reversible photoswitching allows precise control of the density of fluorescing entities, because the equilibrium between the fluorescent ON- and the dark OFF-state can be shifted through optical irradiation at a specific wavelength. Depending on the irradiation intensity, the concentration of the ON-state markers can be up to 1,000 times lower than the actual concentration of the labeled molecular entity. Photoswitching expands the range of single-molecule detection based experiments such as fluorescence fluctuation spectroscopy to large entity concentrations in the micromolar range."],["dc.identifier.doi","10.1002/jemt.20505"],["dc.identifier.gro","3143405"],["dc.identifier.isi","000251868200001"],["dc.identifier.pmid","17661359"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/916"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1059-910X"],["dc.title","Reversible photoswitching enables single-molecule fluorescence fluctuation Spectroscopy at high molecular concentration"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2989"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","2997"],["dc.bibliographiccitation.volume","95"],["dc.contributor.author","Stiel, Andre C."],["dc.contributor.author","Andresen, Martin"],["dc.contributor.author","Bock, Hannes"],["dc.contributor.author","Hilbert, Michael"],["dc.contributor.author","Schilde, Jessica"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Egner, Alexander"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Jakobs, Stefan"],["dc.date.accessioned","2017-09-07T11:48:12Z"],["dc.date.available","2017-09-07T11:48:12Z"],["dc.date.issued","2008"],["dc.description.abstract","Reversibly switchable fluorescent proteins (RSFPs) are GFP-like proteins that may be repeatedly switched by irradiation with light from a fluorescent to a nonfluorescent state, and vice versa. They can be utilized as genetically encodable probes and bear large potential for a wide array of applications, in particular for new protein tracking schemes and subdiffraction resolution microscopy. However, the currently described monomeric RSFPs emit only blue-green or green fluorescence; the spectral window for their use is thus rather limited. Using a semirational engineering approach based on the crystal structure of the monomeric nonswitchable red fluorescent protein mCherry, we generated rsCherry and rsCherryRev. These two novel red fluorescent RSFPs exhibit fluorescence emission maxima at similar to 610 nm. They display antagonistic switching modes, i.e., in rsCherry irradiation with yellow light induces the off-to-on transition and blue light the on-to-off transition, whereas in rsCherryRev the effects of the switching wavelengths are reversed. We demonstrate time-lapse live-cell subdiffraction microscopy by imaging rsCherryRev targeted to the endoplasmic reticulum utilizing the switching and localization of single molecules."],["dc.identifier.doi","10.1529/biophysj.108.130146"],["dc.identifier.gro","3143236"],["dc.identifier.isi","000258826900034"],["dc.identifier.pmid","18658221"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/728"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Generation of monomeric reversibly switchable red fluorescent proteins for far-field fluorescence nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]