Gregor, Carola

[["dc.bibliographiccitation.artnumber","11781"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific reports"],["dc.bibliographiccitation.lastpage","10"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Wegner, Waja"],["dc.contributor.author","Ilgen, Peter"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","van Dort, Joris"],["dc.contributor.author","Mott, Alexander C."],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Willig, Katrin I."],["dc.date.accessioned","2019-07-09T11:44:29Z"],["dc.date.available","2019-07-09T11:44:29Z"],["dc.date.issued","2017-09-18"],["dc.description.abstract","The study of proteins in dendritic processes within the living brain is mainly hampered by the diffraction limit of light. STED microscopy is so far the only far-field light microscopy technique to overcome the diffraction limit and resolve dendritic spine plasticity at superresolution (nanoscopy) in the living mouse. After having tested several far-red fluorescent proteins in cell culture we report here STED microscopy of the far-red fluorescent protein mNeptune2, which showed best results for our application to superresolve actin filaments at a resolution of ~80 nm, and to observe morphological changes of actin in the cortex of a living mouse. We illustrate in vivo far-red neuronal actin imaging in the living mouse brain with superresolution for time periods of up to one hour. Actin was visualized by fusing mNeptune2 to the actin labels Lifeact or Actin-Chromobody. We evaluated the concentration dependent influence of both actin labels on the appearance of dendritic spines; spine number was significantly reduced at high expression levels whereas spine morphology was normal at low expression."],["dc.identifier.doi","10.1038/s41598-017-11827-4"],["dc.identifier.pmid","28924236"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14798"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59023"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","573"],["dc.subject.ddc","612"],["dc.title","In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L01"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","L03"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Herholt, Alexander"],["dc.contributor.author","Rossner, Moritz J."],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:46:54Z"],["dc.date.available","2017-09-07T11:46:54Z"],["dc.date.issued","2014"],["dc.description.abstract","We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 mu m in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology."],["dc.identifier.doi","10.1016/j.bpj.2013.11.1119"],["dc.identifier.gro","3142199"],["dc.identifier.isi","000329407700001"],["dc.identifier.pmid","24411266"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11365"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/5632"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.eissn","1542-0086"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Nanoscopy of Filamentous Actin in Cortical Dendrites of a Living Mouse"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Wegner, Waja"],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Wolf, Fred"],["dc.contributor.author","Willig, Katrin I."],["dc.date.accessioned","2022-04-01T10:00:22Z"],["dc.date.available","2022-04-01T10:00:22Z"],["dc.date.issued","2022"],["dc.description.abstract","Synaptic plasticity underlies long-lasting structural and functional changes to brain circuitry and its experience-dependent remodeling can be fundamentally enhanced by environmental enrichment. It is however unknown, whether and how the environmental enrichment alters the morphology and dynamics of individual synapses. Here, we present a virtually crosstalk-free two-color in vivo stimulated emission depletion (STED) microscope to simultaneously superresolve the dynamics of endogenous PSD95 of the post-synaptic density and spine geometry in the mouse cortex. In general, the spine head geometry and PSD95 assemblies were highly dynamic, their changes depended linearly on their original size but correlated only mildly. With environmental enrichment, the size distributions of PSD95 and spine head sizes were sharper than in controls, indicating that synaptic strength is set more uniformly. The topography of the PSD95 nanoorganization was more dynamic after environmental enrichment; changes in size were smaller but more correlated than in mice housed in standard cages. Thus, two-color in vivo time-lapse imaging of synaptic nanoorganization uncovers a unique synaptic nanoplasticity associated with the enhanced learning capabilities under environmental enrichment."],["dc.identifier.doi","10.7554/eLife.73603"],["dc.identifier.pmid","35195066"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/105414"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/451"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-530"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2050-084X"],["dc.relation.workinggroup","RG Willig (Optical Nanoscopy in Neuroscience)"],["dc.relation.workinggroup","RG Wolf"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.title","Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","577"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Nature communications"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Richardson, Douglas S."],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Winter, Franziska R."],["dc.contributor.author","Urban, Nicolai T."],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:31:10Z"],["dc.date.available","2018-01-17T13:31:10Z"],["dc.date.issued","2017"],["dc.description.abstract","Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the most widely used families of biosensors in microscopy. One key application of pH probes has been to monitor the acidification of vesicles during endocytosis, an essential function that aids in cargo sorting and degradation. Prior to the development of super-resolution fluorescence microscopy (nanoscopy), investigation of endosomal dynamics in live cells remained difficult as these structures lie at or below the ~250 nm diffraction limit of light microscopy. Therefore, to aid in investigations of pH dynamics during endocytosis at the nanoscale, we have specifically designed a family of ratiometric endosomal pH probes for use in live-cell STED nanoscopy.Ratiometric fluorescent pH probes are useful tools to monitor acidification of vesicles during endocytosis, but the size of vesicles is below the diffraction limit. Here the authors develop a family of ratiometric pH sensors for use in STED super-resolution microscopy, and optimize their delivery to endosomes."],["dc.identifier.doi","10.1038/s41467-017-00606-4"],["dc.identifier.pmid","28924139"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16496"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11717"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","SRpHi ratiometric pH biosensors for super-resolution microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Acta Physiologica / Supplement"],["dc.bibliographiccitation.volume","213"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Willig, Katrin I."],["dc.contributor.author","Steffens, Heinz"],["dc.contributor.author","Wegner, W."],["dc.contributor.author","Gregor, Carola"],["dc.date.accessioned","2017-09-07T11:45:51Z"],["dc.date.available","2017-09-07T11:45:51Z"],["dc.date.issued","2015"],["dc.format.extent","18"],["dc.identifier.doi","10.1111/apha.12481"],["dc.identifier.gro","3145538"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/3247"],["dc.notes.intern","lifescience"],["dc.notes.status","public"],["dc.notes.submitter","oschaef1"],["dc.publisher","Wiley-Blackwell"],["dc.relation.conference","94th Annual Meeting of the German Physiological Society"],["dc.relation.eissn","1748-1716"],["dc.relation.eventend","2015-03-07"],["dc.relation.eventlocation","Magdeburg"],["dc.relation.eventstart","2015-03-05"],["dc.relation.issn","1748-1708"],["dc.title","In vivo STED microscopy of the visual cortex of adult mice"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]