Hülsmann, Swen

[["dc.bibliographiccitation.firstpage","1229"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","1241"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Rahman, Jamilur"],["dc.contributor.author","Latal, A. Tobias"],["dc.contributor.author","Besser, Stefanie"],["dc.contributor.author","Hirrlinger, Johannes"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T09:26:26Z"],["dc.date.available","2018-11-07T09:26:26Z"],["dc.date.issued","2013"],["dc.description.abstract","Inhibitory neurons are involved in the generation and patterning of the respiratory rhythm in the adult animal. However, the role of glycinergic neurons in the respiratory rhythm in the developing network is still not understood. Although the complete loss of glycinergic transmission in vivo is lethal, the blockade of glycinergic transmission in slices of the medulla has little effect on pre-Botzinger complex network activity. As 50% of the respiratory rhythmic neurons in this slice preparation are glycinergic, they have to be considered as integrated parts of the network. We aimed to investigate whether glycinergic neurons receive mixed miniature inhibitory postsynaptic currents (mIPSCs) that result from co-release of GABA and glycine. Quantification of mixed mIPSCs by the use of different objective detection methods resulted in a wide range of results. Therefore, we generated traces of mIPSCs with a known distribution of mixed mIPSCs and mono-transmitter-induced mIPSCs, and tested the detection methods on the simulated data. We found that analysis paradigms, which are based on fitting the sum of two mIPSC templates, to be most acceptable. On the basis of these protocols, 2040% of all mIPSCs recorded from respiratory glycinergic neurons are mixed mIPSCs that result from co-release of GABA and glycine. Furthermore, single-cell reverse transcriptase polymerase chain reaction revealed that 46% of glycinergic neurons co-express mRNA of glycine transporter 2 together with at least one marker protein of GABAergic neurons. Our data suggest that significant co-transmission occurs in the pre-Botzinger complex that might be involved in the shaping of synaptic inhibition of respiratory glycinergic neurons."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft (DFG) [HI1414/2-1, HU797/7-1]; CMPB"],["dc.identifier.doi","10.1111/ejn.12136"],["dc.identifier.isi","000317850800003"],["dc.identifier.pmid","23347272"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30298"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0953-816X"],["dc.title","Mixed miniature postsynaptic currents resulting from co-release of glycine and GABA recorded from glycinergic neurons in the neonatal respiratory network"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Stem Cell Research"],["dc.bibliographiccitation.lastpage","154"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Streckfuss-Boemeke, Katrin"],["dc.contributor.author","Vlasov, Alla"],["dc.contributor.author","Huelsmann, Swen"],["dc.contributor.author","Yin, Dongjiao"],["dc.contributor.author","Nayernia, Karim"],["dc.contributor.author","Engel, Wolfgang"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Guan, Kaomei"],["dc.date.accessioned","2017-09-07T11:47:33Z"],["dc.date.available","2017-09-07T11:47:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Recently, we reported the successful establishment of multipotent adult germ-Line stem cells (maGSCs) from cultured adult mouse spermatogonial stem cells. Similar to embryonic stem cells, maGSCs are able to self-renew and differentiate into derivatives of all three germ Layers. These properties make maGSCs a potential cell source for the treatment of neural degenerative diseases. In this study, we describe the generation of maGSC-derived proliferating neural precursor cells using growth factor-mediated neural Lineage induction. The neural precursors were positive for nestin and Sox1 and could be continuously expanded. Upon further differentiation, they formed functional neurons and glial cells, as demonstrated by expression of lineage-restricted genes and proteins and by electrophysiological properties. Characterization of maGSC-derived neurons revealed the generation of specific subtypes, including GABAergic, glutamatergic, serotonergic, and dopaminergic neurons. Electrophysiological analysis revealed passive and active membrane properties and postsynaptic currents, indicating their functional maturation. Functional networks formed at later stages of differentiation, as evidenced by synaptic transmission of spontaneous neuronal activity. In conclusion, our data demonstrate that maGSCs may be used as a new stem cell source for basic research and biomedical. applications. (C) 2008 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.scr.2008.09.001"],["dc.identifier.gro","3143148"],["dc.identifier.isi","000272224500006"],["dc.identifier.pmid","19383419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/630"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1873-5061"],["dc.title","Generation of functional neurons and glia from multipotent adult mouse germ-line stem cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","111"],["dc.bibliographiccitation.journal","Neuroscience"],["dc.bibliographiccitation.lastpage","122"],["dc.bibliographiccitation.volume","347"],["dc.contributor.author","Oku, Yoshitaka"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T10:25:04Z"],["dc.date.available","2018-11-07T10:25:04Z"],["dc.date.issued","2017"],["dc.description.abstract","The topology of the respiratory network in the brainstem has been addressed using different computational models, which help to understand the functional properties of the system. We tested a neural mass model by comparing the result of activation and inhibition of inhibitory neurons in silico with recently published results of optogenetic manipulation of glycinergic neurons [Sherman, et al. (2015) Nat Neurosci 18:408]. The comparison revealed that a five-cell type model consisting of three classes of inhibitory neurons [I-DEC, E-AUG, E-DEC (PI)] and two excitatory populations (pre-I/1) and (I-AUG) neurons can be applied to explain experimental observations made by stimulating or inhibiting inhibitory neurons by light sensitive ion channels. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved."],["dc.identifier.doi","10.1016/j.neuroscience.2017.01.041"],["dc.identifier.isi","000398010100011"],["dc.identifier.pmid","28215988"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/42776"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","1873-7544"],["dc.relation.issn","0306-4522"],["dc.title","A COMPUTATIONAL MODEL OF THE RESPIRATORY NETWORK CHALLENGED AND OPTIMIZED BY DATA FROM OPTOGENETIC MANIPULATION OF GLYCINERGIC NEURONS"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","The Journal of Physiological Sciences"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Lal, Amit"],["dc.contributor.author","Oku, Yoshitaka"],["dc.contributor.author","Hulsmann, Swen"],["dc.contributor.author","Okada, Yasumasa"],["dc.contributor.author","Miwakeichi, Fumikazu"],["dc.contributor.author","Kawai, Shigeharu"],["dc.contributor.author","Tamura, Yoshiyasu"],["dc.contributor.author","Ishiguro, Makio"],["dc.date.accessioned","2018-11-07T08:34:57Z"],["dc.date.available","2018-11-07T08:34:57Z"],["dc.date.issued","2009"],["dc.format.extent","260"],["dc.identifier.isi","000271023101634"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17944"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Tokyo"],["dc.relation.issn","1880-6546"],["dc.title","NON-DETERMINISTIC BREAKDOWN OF THE PREBOTZINGER COMPLEX NEURONAL SYNCHRONICITY MAY LEAD TO QUANTAL SLOWING OF RESPIRATORY RHYTHM"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","108"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Respiratory Physiology & Neurobiology"],["dc.bibliographiccitation.lastpage","114"],["dc.bibliographiccitation.volume","159"],["dc.contributor.author","Winter, Stefan M."],["dc.contributor.author","Hirdinger, Johannes"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T10:57:47Z"],["dc.date.available","2018-11-07T10:57:47Z"],["dc.date.issued","2007"],["dc.description.abstract","We screened transgenic mouse lines with Thy 1.2 promoter-induced expression of fluorescent proteins (FPs) for targeting of respiratory neuronal populations in the medulla oblongata. Respiratory neurons were found to be tagged by FPs within the ventral respiratory column (VRC), the pre-Botzinger complex (preBotC) and the rostral ventral respiratory group (rVRG) interneurons. A subset of neurons in the preBotC, labeled with the enhanced yellow fluorescent protein (EYFP), showed inspiratory activity during whole cell recordings from rhythmic slice preparations. Additionally, a subpopulation of EYFP-labeled preBotC neurons expressed both NK1 - and mu-opioid receptors. Furthermore, the spinal tri.-eminal nucleus, the lateral reticular nucleus (LRT) and the hypoglossal nucleus demonstrated intense EYFP expression whereas other regions of the medulla were devoid of neuronal EYFP labeling (e.g. the nucleus ambiguous). In conclusion, Thy 1.2-FP transgenic mice will facilitate the functional analysis of respiratory-related neurons in the medulla and improve the three dimensional analysis of cells contributing to this important neuronal circuit. (c) 2007 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.resp.2007.05.009"],["dc.identifier.isi","000250604200013"],["dc.identifier.pmid","17616445"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50333"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1569-9048"],["dc.title","Transgenic expression of fluorescent proteins in respiratory neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","193"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Brain Structure and Function"],["dc.bibliographiccitation.lastpage","203"],["dc.bibliographiccitation.volume","220"],["dc.contributor.author","Schnell, Christian"],["dc.contributor.author","Shahmoradi, Ali"],["dc.contributor.author","Wichert, Sven P."],["dc.contributor.author","Mayerl, Steffen"],["dc.contributor.author","Hagos, Yohannes"],["dc.contributor.author","Heuer, Heike"],["dc.contributor.author","Rossner, Moritz J."],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T10:03:38Z"],["dc.date.available","2018-11-07T10:03:38Z"],["dc.date.issued","2015"],["dc.description.abstract","Sulforhodamine 101 (SR101) is widely used for astrocyte identification, though the labeling mechanism remains unknown and the efficacy of labeling in different brain regions is heterogeneous. By combining region-specific isolation of astrocytes followed by transcriptome analysis, two-photon excitation microscopy, and mouse genetics, we identified the thyroid hormone transporter OATP1C1 as the SR101-uptake transporter in hippocampus and cortex."],["dc.identifier.doi","10.1007/s00429-013-0645-0"],["dc.identifier.isi","000348980800014"],["dc.identifier.pmid","24129767"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38516"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Heidelberg"],["dc.relation.issn","1863-2661"],["dc.relation.issn","1863-2653"],["dc.title","The multispecific thyroid hormone transporter OATP1C1 mediates cell-specific sulforhodamine 101-labeling of hippocampal astrocytes"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","202"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Neuroscience Methods"],["dc.bibliographiccitation.lastpage","212"],["dc.bibliographiccitation.volume","183"],["dc.contributor.author","Heck, Christian"],["dc.contributor.author","Kunst, Michael"],["dc.contributor.author","Haertel, Kai"],["dc.contributor.author","Huelsmann, Swen"],["dc.contributor.author","Heinrich, Ralf"],["dc.date.accessioned","2018-11-07T11:23:11Z"],["dc.date.available","2018-11-07T11:23:11Z"],["dc.date.issued","2009"],["dc.description.abstract","Injection of muscarine into the central complex of the grasshopper brain can stimulate species-specific sound production through activation of the phospholipase C-initiated transduction pathway. We introduce a strategy, to label central complex interneurons that are directly stimulated by the injected muscarine and to study their physiology in dissociated primary cell culture. Fluorescent dextranes, co-injected to brain sites where muscarine stimulates sound production, are incorporated from the extracellular space by 3-14 central complex neurons. Most labeled neurons are columnar neurons that express muscarinic acetylcholine receptors. An average of 3-4 dextrame-labeled central complex neurons per brain can be recognised by their fluorescence in dissociated cell cultures. Their function as potential direct targets of previous in vivo pharmacological stimulation of the intact brain was supported by expression of muscarinic receptors in cytomembranes of isolated neuronal cell bodies and muscarine-stimulated calcium responses in vitro. Pharmacological inhibition of phospholipase C function and removal of extracellular calcium indicated that release from inositolphosphate-regulated internal stores mediates the increase of cytosolic calcium concentrations. The experimental procedures described in this study can be applied to any preparation in which focal drug application elicits, terminates or modulates behavior in order to label and physiologically analyse those interneurons within the circuit that serve as direct targets of the injected drug. (C) 2009 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jneumeth.2009.06.032"],["dc.identifier.isi","000270479500014"],["dc.identifier.pmid","19583981"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56143"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0165-0270"],["dc.title","In vivo labeling and in vitro characterisation of central complex neurons involved in the control of sound production"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Anesthesia & Analgesia"],["dc.bibliographiccitation.volume","118"],["dc.contributor.author","Fortuna, Michal G."],["dc.contributor.author","Kugler, Sebastian"],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T09:41:05Z"],["dc.date.available","2018-11-07T09:41:05Z"],["dc.date.issued","2014"],["dc.format.extent","S178"],["dc.identifier.isi","000209827600153"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33649"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.issn","0003-2999"],["dc.title","OPTOGENETIC DISSECTION OF THE NEURONAL CIRCUITS OF THE MOUSE RESPIRATORY NETWORK"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Acta Physiologica"],["dc.bibliographiccitation.volume","216"],["dc.contributor.author","Kono, Y."],["dc.contributor.author","Huelsmann, Swen"],["dc.date.accessioned","2018-11-07T10:17:28Z"],["dc.date.available","2018-11-07T10:17:28Z"],["dc.date.issued","2016"],["dc.identifier.isi","000372285400185"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41231"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.issn","1748-1716"],["dc.relation.issn","1748-1708"],["dc.title","A potential presynaptic role of Glra3 in regulation of glycinergic synaptic inhibition to hypoglossal motoneurons"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","NEURON GLIA BIOLOGY"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Huelsmann, Swen"],["dc.contributor.author","Fresemann, Jens"],["dc.contributor.author","Winter, Stefan M."],["dc.contributor.author","Haertel, Kai"],["dc.date.accessioned","2018-11-07T11:07:12Z"],["dc.date.available","2018-11-07T11:07:12Z"],["dc.date.issued","2007"],["dc.format.extent","S23"],["dc.identifier.isi","000251708800069"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52498"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cambridge Univ Press"],["dc.publisher.place","New york"],["dc.relation.issn","1740-925X"],["dc.title","Modulation of synaptic transmission by astrocytes: astrocytic calcium signals in the respiratory network"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]