Becker, Tamara

[["dc.bibliographiccitation.firstpage","505"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Vogt, Edgar-John"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Roshani, Berit"],["dc.contributor.author","Becker, Tamara"],["dc.contributor.author","Mätz-Rensing, Kerstin"],["dc.contributor.author","Kues, Wilfried A."],["dc.contributor.author","Kügler, Sebastian"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2021-06-01T09:42:32Z"],["dc.date.available","2021-06-01T09:42:32Z"],["dc.date.issued","2021"],["dc.description.abstract","Genetic modification of non-human primates (NHP) paves the way for realistic disease models. The common marmoset is a NHP species increasingly used in biomedical research. Despite the invention of RNA-guided nucleases, one strategy for protein overexpression in NHP is still lentiviral transduction. We generated three male and one female enhanced green fluorescent protein (EGFP)-transgenic founder marmosets via lentiviral transduction of natural preimplantation embryos. All founders accomplished germline transmission of the transgene by natural mating, yielding 20 transgenic offspring together (in total, 45 pups; 44% transgenic). This demonstrates that the transgenic gametes are capable of natural fertilization even when in competition with wildtype gametes. Importantly, 90% of the transgenic offspring showed transgene silencing, which is in sharp contrast to rodents, where the identical transgene facilitated robust EGFP expression. Furthermore, we consistently discovered somatic, but so far, no germ cell chimerism in mixed wildtype/transgenic litters. Somatic cell chimerism resulted in false-positive genotyping of the respective wildtype littermates. For the discrimination of transgenic from transgene-chimeric animals by polymerase chain reaction on skin samples, a chimeric cell depletion protocol was established. In summary, it is possible to establish a cohort of genetically modified marmosets by natural mating, but specific requirements including careful promoter selection are essential."],["dc.identifier.doi","10.3390/cells10030505"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85277"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2073-4409"],["dc.title","Generation and Breeding of EGFP-Transgenic Marmoset Monkeys: Cell Chimerism and Implications for Disease Modeling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2414"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Animals"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Drummer, Charis"],["dc.contributor.author","Münzker, Julia"],["dc.contributor.author","Heistermann, Michael"],["dc.contributor.author","Becker, Tamara"],["dc.contributor.author","Mißbach, Sophie"],["dc.contributor.author","Behr, Rüdiger"],["dc.date.accessioned","2022-10-04T10:21:49Z"],["dc.date.available","2022-10-04T10:21:49Z"],["dc.date.issued","2022"],["dc.description.abstract","Non-human primates (NHPs) serve as embryo donors for embryo collection in order to mimic genetic diseases in humans by genetic modification. Reproductive health of the embryo donors is crucial, and chronic distress needs to be avoided. Embryo retrieval rates (ERR), anti-Müllerian hormone (AMH) concentrations, cortisol levels, and body weight fluctuations were assessed as markers for fertility and distress. With regard to successful embryo retrievals (total n = 667), the animals were either used for extended periods (long-term group; LTG) or only for short periods (short-term group; STG). Retrospective evaluation expectedly showed that animals in the LTG had a higher ERR than animals in the STG (p < 0.0001). Importantly, ERR in the LTG remained stable throughout the experimental period, and high embryo rates were already encountered during the first year of experimental use (p = 0.0002). High ERR were associated with high AMH and low cortisol levels, and minimal body weight fluctuations following anesthesia, indicating a superior ability of the LTG animals to handle distress. We conclude that the long-term experimental use of marmosets does not impair their fertility or health status per se, supporting the view that animal reuse can be in accordance with the 3R-principle, implying reduction, replacement, and refinement in animal experimentation."],["dc.identifier.doi","10.3390/ani12182414"],["dc.identifier.pii","ani12182414"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/114507"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2076-2615"],["dc.relation.orgunit","Deutsches Primatenzentrum"],["dc.title","Performance of Marmoset Monkeys as Embryo Donors Is Reflected by Different Stress-Related Parameters"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]