Stephan, Till

[["dc.bibliographiccitation.firstpage","199"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Nature computational science"],["dc.bibliographiccitation.lastpage","211"],["dc.bibliographiccitation.volume","1"],["dc.contributor.author","Tameling, Carla"],["dc.contributor.author","Stoldt, Stefan"],["dc.contributor.author","Stephan, Till"],["dc.contributor.author","Naas, Julia"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Munk, Axel"],["dc.date.accessioned","2021-12-01T10:55:26Z"],["dc.date.available","2021-12-01T10:55:26Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1038/s43588-021-00050-x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94899"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/242"],["dc.relation","SFB 1456: Mathematik des Experiments: Die Herausforderung indirekter Messungen in den Naturwissenschaften"],["dc.relation","SFB 1456 | Cluster C | C06: Optimal transport based colocalization"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","2662-8457"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.relation.workinggroup","RG Munk"],["dc.title","Colocalization for super-resolution microscopy via optimal transport"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","6"],["dc.contributor.author","Faoro, Raffaele"],["dc.contributor.author","Bassu, Margherita"],["dc.contributor.author","Mejia, Yara X."],["dc.contributor.author","Stephan, Till"],["dc.contributor.author","Dudani, Nikunj"],["dc.contributor.author","Boeker, Christian"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Burg, Thomas P."],["dc.date.accessioned","2018-01-30T08:10:07Z"],["dc.date.available","2018-01-30T08:10:07Z"],["dc.date.issued","2018"],["dc.description.abstract","Cryogenic fluorescent light microscopy of flash-frozen cells stands out by artifact-free fixation and very little photobleaching of the fluorophores used. To attain the highest level of resolution, aberration-free immersion objectives with accurately matched immersion media are required, but both do not exist for imaging below the glass-transition temperature of water. Here, we resolve this challenge by combining a cryoimmersion medium, HFE-7200, which matches the refractive index of room-temperature water, with a technological concept in which the body of the objective and the front lens are not in thermal equilibrium. We implemented this concept by replacing the metallic front-lens mount of a standard bioimaging water immersion objective with an insulating ceramic mount heated around its perimeter. In this way, the objective metal housing can be maintained at room temperature, while creating a thermally shielded cold microenvironment around the sample and front lens. To demonstrate the range of potential applications, we show that our method can provide superior contrast in Escherichia coli and yeast cells expressing fluorescent proteins and resolve submicrometer structures in multicolor immunolabeled human bone osteosarcoma epithelial (U2OS) cells at [Formula: see text]C."],["dc.identifier.doi","10.1073/pnas.1717282115"],["dc.identifier.pmid","29358380"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11902"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","1091-6490"],["dc.title","Aberration-corrected cryoimmersion light microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","289"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Annual Review of Biophysics"],["dc.bibliographiccitation.lastpage","308"],["dc.bibliographiccitation.volume","49"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Stephan, Till"],["dc.contributor.author","Ilgen, Peter"],["dc.contributor.author","Brüser, Christian"],["dc.date.accessioned","2021-04-14T08:27:46Z"],["dc.date.available","2021-04-14T08:27:46Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1146/annurev-biophys-121219-081550"],["dc.identifier.pmid","32092283"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82396"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/14"],["dc.identifier.url","https://sfb1190.med.uni-goettingen.de/production/literature/publications/105"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/50"],["dc.identifier.url","https://for2848.gwdguser.de/literature/publications/24"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente"],["dc.relation","SFB 1190 | P01: Untersuchung der Unterschiede in der Zusammensetzung, Funktion und Position von individuellen MICOS Komplexen in einzelnen Säugerzellen"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A05: Mitochondriale Heterogenität in Synapsen"],["dc.relation","FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala"],["dc.relation","FOR 2848 | P04: Analyse der räumlichen Organisation der OXPHOS Assemblierung in Säugerzellen"],["dc.relation.eissn","1936-1238"],["dc.relation.issn","1936-122X"],["dc.relation.workinggroup","RG Jakobs (Structure and Dynamics of Mitochondria)"],["dc.title","Light Microscopy of Mitochondria at the Nanoscale"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","overview_ja"],["dspace.entity.type","Publication"]]