Betz, Andrea

[["dc.bibliographiccitation.firstpage","123"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","136"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Betz, Andrea"],["dc.contributor.author","Ashery, Uri"],["dc.contributor.author","Rickmann, Michael"],["dc.contributor.author","Augustin, Iris"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Südhof, Thomas C."],["dc.contributor.author","Rettig, Jens"],["dc.contributor.author","Brose, Nils"],["dc.date.accessioned","2017-09-07T11:48:09Z"],["dc.date.available","2017-09-07T11:48:09Z"],["dc.date.issued","1998"],["dc.description.abstract","Munc13-1, a mammalian homolog of C. elegans unc-13p, is thought to be involved in the regulation of synaptic transmission. We now demonstrate that Munc13-1 is a presynaptic high-affinity phorbol ester and diacylglycerol receptor with ligand affinities similar to those of protein kinase C. Munc13-1 associates with the plasma membrane in response to phorbol ester binding and acts as a phorbol ester-dependent enhancer of transmitter release when overexpressed presynaptically in the Xenopus neuromuscular junction. These observations establish Munc13-1 as a novel presynaptic target of the diacylglycerol second messenger pathway that acts in parallel with protein kinase C to regulate neurotransmitter secretion."],["dc.identifier.doi","10.1016/S0896-6273(00)80520-6"],["dc.identifier.gro","3144539"],["dc.identifier.isi","000075061900012"],["dc.identifier.pmid","9697857"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2174"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Cell Press"],["dc.relation.eissn","1097-4199"],["dc.relation.issn","0896-6273"],["dc.title","Munc13-1 is a presynaptic phorbol ester receptor that enhances neurotransmitter release"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3586"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","EMBO Journal"],["dc.bibliographiccitation.lastpage","3596"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Ashery, Uri"],["dc.contributor.author","Varoqueaux, Frederique"],["dc.contributor.author","Voets, T."],["dc.contributor.author","Betz, Andrea"],["dc.contributor.author","Koch, H."],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Rettig, Jens"],["dc.date.accessioned","2017-09-07T11:46:47Z"],["dc.date.available","2017-09-07T11:46:47Z"],["dc.date.issued","2000"],["dc.description.abstract","In chromaffin cells the number of large dense-core vesicles (LDCVs) which can be released by brief, intense stimuli represents only a small fraction of the 'morphologically docked' vesicles at the plasma membrane. Recently, it was shown that Munc13-1 is essential for a post-docking step of synaptic vesicle fusion, To investigate the role of Munc13-1 in LDCV exocytosis, we overexpressed Munc13-1 in chromaffin cells and stimulated secretion by flash photolysis of caged calcium. Both components of the exocytotic burst, which represent the fusion of release-competent vesicles, were increased by a factor of three. The sustained component, which represents vesicle maturation and subsequent fusion, was increased by the same factor. The response to a second flash, however, was greatly reduced, indicating a depletion of release-competent vesicles. Since there was no apparent change in the number of docked vesicles, we conclude that Munc13-1 acts as a priming factor by accelerating the rate constant of vesicle transfer from a pool of docked, but unprimed vesicles to a pool of release-competent, primed vesicles."],["dc.identifier.doi","10.1093/emboj/19.14.3586"],["dc.identifier.gro","3144373"],["dc.identifier.isi","000088447000008"],["dc.identifier.pmid","10899113"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1990"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0261-4189"],["dc.title","Munc13-1 acts as a priming factor for large dense-core vesicles in bovine chromaffin cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","525"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","532"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Ashery, Uri"],["dc.contributor.author","Betz, Andrea"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Rettig, Jens"],["dc.contributor.author","Xu, Tao"],["dc.date.accessioned","2017-09-07T11:47:29Z"],["dc.date.available","2017-09-07T11:47:29Z"],["dc.date.issued","1999"],["dc.description.abstract","We have expanded the use of the Semliki Forest virus (SFV) by infecting chromaffin tells with synaptic proteins at high efficiency. Using the SFV gene expression system, up to 40 % of cultured bovine chromaffin cells express the protein of interest within 12-48 h after infection. In order to learn about the basic physiological properties of infected cells, we performed membrane capacitance measurements using the whole-cell patch-clamp technique and monitored catecholamine release with amperometry. We found that chromaffin cells infected with green fluorescent protein (GFP) were comparable to control cells in intracellular calcium concentrations ([Ca2+](i)), leak currents and cell sizes, In response to depolarization, calcium currents were elicited and the cells secreted catecholamine, Comparison of the calcium current amplitude and the size of the readily releasable pool of vesicles revealed a small decrease in these parameters compared to control cells, The refilling kinetics after pool depletion, however, were not altered, Overexpressed munc13-1 translocates to the plasma membrane in response to phorbol esters, an effect that is also observed in fibroblasts transfected with conventional methods. Thus, the use of the SFV gene expression system to infect chromaffin cells represents a major improvement in infection efficiency compared to other methods. It opens up new opportunities to introduce synaptic proteins into chromaffin cells and study their role in secretion."],["dc.identifier.doi","10.1016/s0171-9335(99)80017-x"],["dc.identifier.gro","3144450"],["dc.identifier.isi","000082460200001"],["dc.identifier.pmid","10494858"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2075"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","0171-9335"],["dc.relation.issn","0171-9335"],["dc.title","An efficient method for infection of adrenal chromaffin cells using the Semliki Forest virus gene expression system"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","525"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","European Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","532"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Ashery, Uri"],["dc.contributor.author","Betz, Andrea"],["dc.contributor.author","Xu, Tao"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Rettig, Jens"],["dc.date.accessioned","2022-03-01T11:45:32Z"],["dc.date.available","2022-03-01T11:45:32Z"],["dc.date.issued","1999"],["dc.identifier.doi","10.1016/S0171-9335(99)80017-X"],["dc.identifier.pii","S017193359980017X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103364"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0171-9335"],["dc.title","An efficient method for infection of adrenal chromaffin cells using the Semliki Forest virus gene express1on system"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","183"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","196"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Betz, Andrea"],["dc.contributor.author","Thakur, P"],["dc.contributor.author","Junge, Harald J."],["dc.contributor.author","Ashery, Uri"],["dc.contributor.author","Rhee, Jeong-Seop"],["dc.contributor.author","Scheuss, V."],["dc.contributor.author","Rosenmund, C."],["dc.contributor.author","Rettig, Jens"],["dc.contributor.author","Brose, Nils"],["dc.date.accessioned","2017-09-07T11:46:08Z"],["dc.date.available","2017-09-07T11:46:08Z"],["dc.date.issued","2001"],["dc.description.abstract","Synaptic neurotransmitter release is restricted to active zones, where the processes of synaptic vesicle tethering, priming to fusion competence, and Ca2+-triggered fusion are taking place in a highly coordinated manner, We show that the active zone components Munc13-1, an essential vesicle priming protein, and RIM1, a Rab3 effector with a putative role in vesicle tethering, interact functionally. Disruption of this interaction causes a loss of fusion-competent synaptic vesicles, creating a phenocopy of Munc13-1-deficient neurons. RIM1 binding and Vesicle priming are mediated by two distinct structural modules of Munc13-1. The Munc13-1/RIM1 interaction may create a functional link between synaptic vesicle tethering and priming, or it may regulate the priming reaction itself, thereby determining the number of fusion-competent vesicles."],["dc.identifier.doi","10.1016/S0896-6273(01)00272-0"],["dc.identifier.gro","3144292"],["dc.identifier.isi","000168412900020"],["dc.identifier.pmid","11343654"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1899"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0896-6273"],["dc.title","Functional interaction of the active zone proteins Munc13-1 and RIM1 in synaptic vesicle priming"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]