Hoppert, Michael

[["dc.bibliographiccitation.firstpage","1402"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Microorganisms"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Arif, Sania"],["dc.contributor.author","Willenberg, Corinna"],["dc.contributor.author","Dreyer, Annika"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Hoppert, Michael"],["dc.date.accessioned","2021-09-01T06:43:04Z"],["dc.date.available","2021-09-01T06:43:04Z"],["dc.date.issued","2021"],["dc.description.abstract","The hydrothermal steam environment of Sasso Pisano (Italy) was selected to investigate the associated microbial community and its metabolic potential. In this context, 16S and 18S rRNA gene partial sequences of thermophilic prokaryotes and eukaryotes inhabiting hot springs and fumaroles as well as mesophilic microbes colonising soil and water were analysed by high-throughput amplicon sequencing. The eukaryotic and prokaryotic communities from hot environments clearly differ from reference microbial communities of colder soil sites, though Ktedonobacteria showed high abundances in various hot spring samples and a few soil samples. This indicates that the hydrothermal steam environments of Sasso Pisano represent not only a vast reservoir of thermophilic but also mesophilic members of this Chloroflexi class. Metabolic functional profiling revealed that the hot spring microbiome exhibits a higher capability to utilise methane and aromatic compounds and is more diverse in its sulphur and nitrogen metabolism than the mesophilic soil microbial consortium. In addition, heavy metal resistance-conferring genes were significantly more abundant in the hot spring microbiome. The eukaryotic diversity at a fumarole indicated high abundances of primary producers (unicellular red algae: Cyanidiales), consumers (Arthropoda: Collembola sp.), and endoparasite Apicomplexa (Gregarina sp.), which helps to hypothesise a simplified food web at this hot and extremely nutrient-deprived acidic environment."],["dc.description.abstract","The hydrothermal steam environment of Sasso Pisano (Italy) was selected to investigate the associated microbial community and its metabolic potential. In this context, 16S and 18S rRNA gene partial sequences of thermophilic prokaryotes and eukaryotes inhabiting hot springs and fumaroles as well as mesophilic microbes colonising soil and water were analysed by high-throughput amplicon sequencing. The eukaryotic and prokaryotic communities from hot environments clearly differ from reference microbial communities of colder soil sites, though Ktedonobacteria showed high abundances in various hot spring samples and a few soil samples. This indicates that the hydrothermal steam environments of Sasso Pisano represent not only a vast reservoir of thermophilic but also mesophilic members of this Chloroflexi class. Metabolic functional profiling revealed that the hot spring microbiome exhibits a higher capability to utilise methane and aromatic compounds and is more diverse in its sulphur and nitrogen metabolism than the mesophilic soil microbial consortium. In addition, heavy metal resistance-conferring genes were significantly more abundant in the hot spring microbiome. The eukaryotic diversity at a fumarole indicated high abundances of primary producers (unicellular red algae: Cyanidiales), consumers (Arthropoda: Collembola sp.), and endoparasite Apicomplexa (Gregarina sp.), which helps to hypothesise a simplified food web at this hot and extremely nutrient-deprived acidic environment."],["dc.description.sponsorship","DAAD Doctoral Scholarship"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/microorganisms9071402"],["dc.identifier.pii","microorganisms9071402"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89213"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.eissn","2076-2607"],["dc.relation.orgunit","Institut für Mikrobiologie und Genetik"],["dc.rights","CC BY 4.0"],["dc.title","Sasso Pisano Geothermal Field Environment Harbours Diverse Ktedonobacteria Representatives and Illustrates Habitat-Specific Adaptations"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4883"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","Biogeosciences"],["dc.bibliographiccitation.lastpage","4902"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Arif, Sania"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Schliekmann, Elias"],["dc.contributor.author","Reimer, Andreas"],["dc.contributor.author","Arp, Gernot"],["dc.contributor.author","Hoppert, Michael"],["dc.date.accessioned","2022-11-01T10:17:31Z"],["dc.date.available","2022-11-01T10:17:31Z"],["dc.date.issued","2022"],["dc.description.abstract","Abstract. The Kilianstollen Marsberg (Rhenish Massif, Germany) has\r\nbeen extensively mined for copper ores, dating from early medieval period\r\nuntil 1945. The exposed organic-rich alum shale rocks influenced by the\r\ndiverse mine drainages at an ambient temperature of 10 ∘C could\r\nnaturally enrich biogeochemically distinct heavy metal resistant microbiota.\r\nThis amplicon-sequence-based study evaluates the microbially colonized\r\nsubterranean rocks of the abandoned copper mine Kilianstollen to\r\ncharacterize the colonization patterns and biogeochemical pathways of\r\nindividual microbial groups. Under the selective pressure of the heavy metal\r\ncontaminated environment at illuminated sites, Chloroflexi (Ktedonobacteria) and Cyanobacteria (Oxyphotobacteria) build up\r\nwhitish–greenish biofilms. In contrast, Proteobacteria, Firmicutes and Actinobacteria dominate rocks around the\r\nuncontaminated spring water streams. The additional metagenomic analysis\r\nrevealed that the heavy metal resistant microbiome was evidently involved in\r\nredox cycling of transition metals (Cu, Zn, Co, Ni, Mn, Fe, Cd, Hg). No\r\ndeposition of metals or minerals, though, was observed by transmission\r\nelectron microscopy in Ktedonobacteria biofilms which may be indicative for the presence of\r\ndifferent detoxification pathways. The underlying heavy metal resistance\r\nmechanisms, as revealed by analysis of metagenome-assembled genomes, were\r\nmainly attributed to transition metal efflux pumps, redox enzymes,\r\nvolatilization of Hg, methylated intermediates of As3+, and reactive\r\noxygen species detoxification pathways."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2022"],["dc.identifier.doi","10.5194/bg-19-4883-2022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116831"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","1726-4189"],["dc.rights","CC BY 4.0"],["dc.title","Composition and niche-specific characteristics of microbial consortia colonizing Marsberg copper mine in the Rhenish Massif"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","unpublished"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Microbiology Resource Announcements"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Arif, Sania"],["dc.contributor.author","Nacke, Heiko"],["dc.contributor.author","Hoppert, Michael"],["dc.date.accessioned","2021-06-01T10:47:35Z"],["dc.date.available","2021-06-01T10:47:35Z"],["dc.date.issued","2021"],["dc.description.abstract","We sequenced the metagenome of a biofilm collected near a leachate stream of the Marsberg copper mine (Germany) and reconstructed eight metagenome-assembled genomes. These genomes yield copper resistance through Cu(I) oxidation via multiple copper oxidases and extrusion through copper-exporting P-type ATPases."],["dc.description.abstract","ABSTRACT We sequenced the metagenome of a biofilm collected near a leachate stream of the Marsberg copper mine (Germany) and reconstructed eight metagenome-assembled genomes. These genomes yield copper resistance through Cu(I) oxidation via multiple copper oxidases and extrusion through copper-exporting P-type ATPases."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1128/MRA.01253-20"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85653"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2576-098X"],["dc.relation.orgunit","Abteilung Allgemeine Mikrobiologie"],["dc.relation.orgunit","Institut für Mikrobiologie und Genetik"],["dc.rights","CC BY 4.0"],["dc.title","Metagenome-Assembled Genome Sequences of a Biofilm Derived from Marsberg Copper Mine"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e00819-20"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","40"],["dc.bibliographiccitation.journal","Microbiology Resource Announcements"],["dc.bibliographiccitation.lastpage","3"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Winkler, Lucia"],["dc.contributor.author","Münker, Marc F."],["dc.contributor.author","Brunotte, Susanne"],["dc.contributor.author","Rohlmann, Lina"],["dc.contributor.author","Diez Alfageme, Alvaro"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Hoppert, Michael"],["dc.contributor.author","Reitner, Joachim"],["dc.contributor.author","Nacke, Heiko"],["dc.date.accessioned","2020-10-08T11:07:42Z"],["dc.date.accessioned","2021-10-27T13:14:04Z"],["dc.date.available","2020-10-08T11:07:42Z"],["dc.date.available","2021-10-27T13:14:04Z"],["dc.date.issued","2020"],["dc.description.abstract","We sequenced the metagenome of an anoxygenic photosynthetic consortium originating from pond water and reconstructed four metagenome-assembled genomes. These genomes include Desulfocapsa, Paludibacter, Lamprocystis, and Rhodocyclaceae representatives and indicate the presence of genes for dissimilatory sulfate reduction and oxidation of reduced sulfur compounds."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2020"],["dc.identifier.doi","10.1128/MRA.00819-20"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17589"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91829"],["dc.language.iso","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.eissn","2576-098X"],["dc.relation.orgunit","Fakultät für Geowissenschaften und Geographie"],["dc.rights","CC BY 4.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Metagenome-Assembled Genome Sequences from an Anoxygenic Photosynthetic Consortium Involved in Sulfur Cycling"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]